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      • KCI등재

        Presentation of a Novel E-Core Transverse-Flux Permanent Magnet Linear Motor and Its Magnetic Field Analysis Based on Schwarz-Christoffel Mapping Method

        Dong-Shan Fu,Yan-Liang Xu 대한전기학회 2017 Journal of Electrical Engineering & Technology Vol.12 No.5

        In order to overcome the manufacturing difficulty of the transverse-flux permanent magnet linear motor (TFPMLM) and enhance its performance much better, a novel TFPMLM with Ecore and 3 dimension (3D) magnetic structures is proposed in this paper. Firstly, its basic structure and operating principle are presented. Then the equivalent 2D configuration of the TFPMLM is transformed, so that the Schwarz-Christoffel (SC) mapping method can be used to analyze the motor. Furthermore, the air gap flux density distribution is solved by SC mapping method, based on which, the EMF waveform, no-load cogging force waveform and load force waveform are obtained. Finally, the prototyped TLPMLM is manufactured and the results are obtained from the experiment and 3D FEM, respectively, which are used to compare with those from SC mapping method.

      • KCI등재

        Baekduia soli gen. nov., sp. nov., a novel bacterium isolated from the soil of Baekdu Mountain and proposal of a novel family name, Baekduiaceae fam. nov.

        Dong-Shan An,Muhammad Zubair Siddiqi,김경호,Hong Shan Yu,임완택 한국미생물학회 2018 The journal of microbiology Vol.56 No.1

        A taxonomic study was conducted on BR7-21T, a bacterial strain isolated from the soil of a ginseng field in Baekdu Mountain. Comparative studies of the 16S rRNA gene sequence showed that the isolate was most closely related to Conexibacter woesei DSM 14684T, Solirubrobacter pauli ATCC BAA-492T, Patulibacter minatonensis JCM 12834T, with 93.8%, 92.4%, and 91.5% sequence similarity, respectively; each genus represented a family in the order Solirubrobacterales. Strain BR7-21T was Gram-reaction positive, non-spore forming, aerobic, non-motile, and short rod-shaped. It grew well on half-strength R2A medium. The G + C content of the genomic DNA was 73.9%. It contained meso-diaminopimelic acid in the cell wall and the major menaquinones were MK-7(H4) and MK-8(H4). The major fatty acids were summarized as (C16:1 ω7c/iso-C15:0 2-OH), iso-C16:0, and C17:0 cyclo. On the basis of polyphasic evidence, it was proposed that strain BR7- 21T should be placed in a new genus and species, for which the name Baekduia soli gen. nov., sp. nov. was proposed with the type strain BR7-21T (= KCTC 22257T = LMG 24797T). The family Baekduiaceae fam. nov. is proposed to encompass the genus Baekduia gen. nov.

      • KCI등재

        Analysis on Response Prediction of a Single Pile and Pile Groups Based on the Runge-Kutta Method

        Dong-dong Pan,Qian-qing Zhang,Shan-wei Liu,Shi-min Zhang 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.1

        The conventional load-transfer approach can not consider the interaction of piles, and can not be directly used in the analysis of the response of pile groups. The interactive effects among piles can be taken into account in the shear displacement method based on the principle of superposition. In the present analysis, the shaft displacement of a single pile at a given depth is assumed to be composed of the pile-soil relative displacement developed at the disturbed soil around pile and the elastic vertical soil displacement developed in the soil mass. A new load-transfer function which is very similar to a hyperbolic model is then established to describe the relationship between unit skin friction and shaft displacement at a given depth. Furthermore, a hyperbolic model is used to simulate the relationship between unit end resistance and pile end displacement. As to the analysis of the response of pile groups, the interactive effects among piles are considered by using the shear displacement method. For an individual pile in pile groups, new load-transfer functions are established to capture the relationship between unit skin friction and shaft displacement and the relationship between end resistance and pile end displacement. Based on the fourth order Runge-Kutta method, a modified loadtransfer method is proposed to analyze the response of pile groups considering the interactive effects among piles. The reliability of the present method is checked by using comparisons of the present calculated results, the measured results and the computed values derived from other methods.

      • KCI등재

        Cough Inhibition Activity of Schisandra chinensis in Guinea Pigs

        Shan Zhong,Li-Ping Bai,Xiao-Dong Liu,Die-Yi Cai,Lee-Fong Yau,Chu-Qin Huang,Jia-Qi Zhang,Ke-Fang Lai,Nan-Shan Zhong 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.4

        Chronic cough is very common in respiratory clinics, and no effective drugs are available. Schisandra chinensis (Turcz.) Baill. (S. chinensis), an important traditional Chinese medicine, has been extensively prescribed for patients with a persistent cough. Preliminary research indicated that 95% ethanol extracts (EE) of S. chinensis showed remarkable antitussive activity in guinea pigs exposed to cigarette smoke (CS). To find out the antitussive ingredients of S. chinensis, EE was divided into four fractions according to the polarity: petroleum ether extract (PEE), ethyl acetate extract (ECE), n-butyl alcohol extract, and residue extract. The antitussive, antioxidant, and anti-inflammatory effects of the four fractions were evaluated in a guinea pig model of cough hypersensitivity induced by CS exposure. Eighteen main constituents of the two effective fractions, PEE and ECE, were identified using ultra-high-pressure liquid chromatography electronic spray ion time-of-flight mass spectrometry. The cough inhibition activities of compound 1, 3, 9, 10, 17 were evaluated on citric acid induced acute cough guinea pigs. The results showed that the antitussive activity of EE was almost all contained in PEE and ECE. The 16 major peaks in PEE were identified as 15 lignans (1–12 and 14–16) and 1 triterpene (compound 13), and 3 major peaks (1, 17, and 18) in ECE were also identified as lignans. Three doses of five compounds brought about a significant decrease in number of cough efforts (P < .01), and the cough inhibition rates were between 40.9% and 85.1%. Therefore, lignans are the antitussive ingredients of S. chinensis.

      • KCI등재

        Gram-Scale Production of Ginsenoside F1 Using a Recombinant Bacterial β-Glucosidase

        ( Dong-shan An ),( Chang-hao Cui ),( Muhammad Zubair Siddiqi ),( Hong Shan Yu ),( Feng-xie Jin ),( Song-gun Kim ),( Wan-taek Im ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.9

        Naturally occurring ginsenoside F1 (20-O-β-D-glucopyranosyl-20(S)-protopanaxatriol) is rare. Here, we produced gram-scale quantities of ginsenoside F1 from a crude protopanaxatriol saponin mixture comprised mainly of Re and Rg1 through enzyme-mediated biotransformation using recombinant β-glucosidase (BgpA) cloned from a soil bacterium, Terrabacter ginsenosidimutans Gsoil 3082<sup>T</sup>. In a systematic step-by-step process, the concentrations of substrate, enzyme, and NaCl were determined for maximal production of F1. At an optimized NaCl concentration of 200 mM, the protopanaxatriol saponin mixture (25 mg/ml) was incubated with recombinant BgpA (20 mg/ml) for 3 days in a 2.4 L reaction. Following octadecylsilyl silica gel column chromatography, 9.6 g of F1 was obtained from 60 g of substrate mixture at 95% purity, as assessed by chromatography. These results represent the first report of gram-scale F1 production via recombinant enzyme-mediated biotransformation.

      • SCIESCOPUSKCI등재
      • SCIEKCI등재

        Recombinant expression of a novel antimicrobial peptide consisting of human α-defensin 5 and Mytilus coruscus mytilin-1 in Escherichia coli

        Shan, Ying,Dong, Ying,Jiang, Dong 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.6

        Antibiotic peptides are a battery of broad-spectrum antibacterial cationic polypeptides widely distributed in the plant and animal kingdoms. Among them, the human defensins are the first line of defense against pathogens and Mytilin, which is isolated from mussel serum, plays a key role in the mussel defense system. The antibacterial activity of these two peptides is generally ascribed to their overall positive charge, which enables them to disrupt bacterial membrane integrity and function. The aim of this study was to develop an effective method for the biosynthesis of a fusion peptide containing human a-defensin 5 (HD5) and Mytilin-1 in Escherichia coli to improve the antimicrobial activity. The individual HD5 and Mytilin-1 peptides were also synthesized for comparison with the fusion peptide. All the peptides, expressed as soluble fusions with the peptide thioredoxin, were isolated using a three-step purification strategy involving nickel-Sepharose chromatography, enterokinase cleavage, and cationic exchange chromatography. The identity of the peptides was confirmed by SDS-PAGE. Antimicrobial activity assays demonstrated that all the recombinant peptides had strong bactericidal properties and that the HD5 and Mytilin-1 fusion protein displayed higher activity against E. coli and Staphylococcus aureus. The results of this study provide a platform for the development of novel cationic peptides for biological studies.

      • KCI등재

        Recombinant expression of a novel antimicrobial peptide consisting of human a-defensin 5 and Mytilus coruscus mytilin-1 in Escherichia coli

        Ying Shan,Ying Dong,Dong Jiang 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.6

        Antibiotic peptides are a battery of broadspectrum antibacterial cationic polypeptides widely distributed in the plant and animal kingdoms. Among them, the human defensins are the first line of defense against pathogens and Mytilin, which is isolated from mussel serum, plays a key role in the mussel defense system. The antibacterial activity of these two peptides is generally ascribed to their overall positive charge, which enables them to disrupt bacterial membrane integrity and function. The aim of this study was to develop an effective method for the biosynthesis of a fusion peptide containing human a-defensin 5 (HD5) and Mytilin-1 in Escherichia coli to improve the antimicrobial activity. The individual HD5 and Mytilin-1 peptides were also synthesized for comparison with the fusion peptide. All the peptides, expressed as soluble fusions with the peptide thioredoxin, were isolated using a three-step purification strategy involving nickel- Sepharose chromatography, enterokinase cleavage, and cationic exchange chromatography. The identity of the peptides was confirmed by SDS-PAGE. Antimicrobial activity assays demonstrated that all the recombinant peptides had strong bactericidal properties and that the HD5 and Mytilin-1 fusion protein displayed higher activity against E. coli and Staphylococcus aureus. The results of this study provide a platform for the development of novel cationic peptides for biological studies.

      • SCIESCOPUSKCI등재

        Presentation of a Novel E-Core Transverse-Flux Permanent Magnet Linear Motor and Its Magnetic Field Analysis Based on Schwarz-Christoffel Mapping Method

        Fu, Dong-Shan,Xu, Yan-Liang The Korean Institute of Electrical Engineers 2017 Journal of Electrical Engineering & Technology Vol.12 No.5

        In order to overcome the manufacturing difficulty of the transverse-flux permanent magnet linear motor (TFPMLM) and enhance its performance much better, a novel TFPMLM with E-core and 3 dimension (3D) magnetic structures is proposed in this paper. Firstly, its basic structure and operating principle are presented. Then the equivalent 2D configuration of the TFPMLM is transformed, so that the Schwarz-Christoffel (SC) mapping method can be used to analyze the motor. Furthermore, the air gap flux density distribution is solved by SC mapping method, based on which, the EMF waveform, no-load cogging force waveform and load force waveform are obtained. Finally, the prototyped TLPMLM is manufactured and the results are obtained from the experiment and 3D FEM, respectively, which are used to compare with those from SC mapping method.

      • KCI등재

        종교와 사고 경향(분석적ᐨ종합적 사고 : Analytic-Holistic Thinking)이 노스탤지어 광고에 미치는 영향

        이동환(Dong Hwan Lee),유산산(Shan Shan Liu),전종우(Jong Woo Jun) SBS 2018 미디어경제와 문화 Vol.16 No.1

        본 연구는 수용자의 종교와 사고 경향이 노스탤지어 광고에 미치는 영향이 어떠한지 알아보았다. 결과적으로 수용자의 종교는 사고 경향에, 사고 경향은 노스탤지어 광고의 인식에 영향을 미쳤다. 그리고 노스탤지어 인식은 종교와 사고 경향과의 상호작용하에 광고 태도, 브랜드 태도, 구매 의도에 영향을 미쳤다. 이러한 연구 결과로 기존 사고 경향에 관한 연구들의 선행변인으로 작용할 수 있는 종교의 영향력을 알 수 있다. 다시 말해 본문에 제시한 니스벳 등의 연구에서 동서양의 사고 경향의 차이를 동양인은 종합적 사고 경향이 있으며 서양인은 분석적 사고 경향이 있다고 제시하였으며 다수의 국내 연구에서도 이에 동의하였는데, 그 사고 경향의 원인으로서 종교가 기능할 수 있다는 것이다. 이러한 결과는 다른 연구나 혹은 실무에 있어서 소비자의 세분화와 관련된 단서를 제공할 수 있다. The purpose of this study is to investigate the effect of audience’s religion and tendency of thinking on nostalgic advertising. As a result, the religion of audiences influenced the tendency of thinking, and the tendency of thinking influenced the perception of nostalgic advertising. In addition, nostalgia perception interacted with religion and tendency of thinking, and affected attitudes toward advertising, attitudes toward the brand, and purchase intentions. In sum, we found that religion act as an antecedent variable of the tendency of thinking. Nisbett proposed that oriental people have tendency of holistic thinking and western people have analytic thinking tendency. In this study, we found that holistic thingking could function as the cause of the nostalgic advertising. We also found the interaction effects between tendency of thingking and religion. These results can provide clues related to consumer segmentation in other studies and practices.

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