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Dong-Hao Zhang,Ya-Qiong Li,Li-Juan Peng,Na Chen 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.5
Poly(styrene-acrylic acid) magnetic microsphereswith an average diameter of 2 μm were successfullyprepared and used as carriers to immobilize lipase. Lipaseimmobilized on microspheres with no spacer arm exhibitedlow activities, which were attributed to steric hindranceon the lipase conformation. To avoid steric effects,ethylenediamine and poly(ethylene glycol) (PEG) 400/800/4000 were utilized as spacer arms to bind the lipase tothe microspheres. The immobilized lipase activities wereimproved using PEG 800/4000 as a spacer arm. Furthermore,the influence of enzyme loading on lipase activity wasinvestigated, and the results indicated that enzymeoverloading could exert steric effect on lipase activity. Thedegree of PEG modification was demonstrated to affectlipase activity because excess PEG on the surface ofmicrospheres could interact with lipase due to its mobility,consequently reducing lipase activity.
Hao Dong,Hao-Qin Zhang,Gang Liang,Bing-Xing Ren,Xiao-Long Zhao 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.4
Twin rotors concentric face-gear power-split transmission system (TRCFGPSTS) is a new and more advanced main reducer configuration of the high-speed helicopter. Its loadsharing performance between different branches has a great impact on the service life performance. Its load distribution is complex, and the research on the influence law of error on loadsharing performance is not clear. Therefore, to study the influence of system error and pinions floating on the mechanical properties of the system, the static load-sharing mathematical model of the system was established. The load-sharing model comprehensively considers the factors of manufacturing error (ME) and installation error (IE), meshing stiffness, torsional stiffness, support stiffness and member floating. The coordination conditions of torsional angle elastic deformation of the system with central displacement offset and the mechanical balance conditions under the action of elastic support and floating support are derived, which reflects the change law of load distribution from the perspective of the system as a whole. The load-sharing model is solved by the least square method. The load-sharing coefficient (LSC) of the system is obtained. The influence of ME, IE and floating pinions on load-sharing performance is analyzed. The results show that the LSC shows irregular periodic fluctuation under the combined action of error, and the LSC is 1.212. Under the single influence of ME, the LSC also shows irregular periodic fluctuation, and the LSC is 1.610. Under the single influence of IE, the LSC presents a constant influence law, and the LSC is 1.326. The error of each gear in the III-stage has the most obvious influence on the load-sharing characteristics. The LSC of I-stage and II-stage floating pinion under the single influence are 1.113 and 1.120, respectively. The floating gear can significantly improve the load-sharing performance, and meet the load-sharing at the ±5 % service requirement.
Kinetics of Enzymatic Synthesis of L-ascorbyl Acetate by Lipozyme TLIM and Novozym 435
Dong-Hao Zhang,Ya-Qiong Li,Chao Li,Yv-Qin Lv,Yang-L 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.1
L-ascorbyl acetate was synthesized through lipase-catalyzed esterification using Lipozyme TLIM and Novozym 435. Four solvents, including methanol, ethanol,acetonitrile, and acetone were investigated for the reaction,and acetone and acetonitrile were found to be suitable reaction media. The influences of several parameters such as water activity (aw), substrate molar ratio, enzyme loading,and reaction temperature on esterification of Lascorbic acid were systematically and quantitatively analyzed. Through optimizing the reaction, lipase-catalyzed esterification of L-ascorbic acid gave a maximum conversion of 99%. The results from using Lipozyme TLIM and Novozym 435 as biocatalysts both showed that aw was an important factor for the conversion of L-ascorbic acid. The effect of pH value on lipase-catalyzed L-ascorbic acid esterification in acetone was also investigated. Furthermore,results from a kinetic characterization of Lipozyme TLIM were compared with those for Novozym 435, and suggested that the maximum reaction rate for Lipozyme TLIM was greater than that for Novozym 435, while the enzyme affinity for substrate was greater for Novozym 436.
Chen Hao,Zhang Dong-Ming,Zhang Zhi-Ping,Li Ming-Zhang,Wu Hai-Feng 한국유전학회 2021 Genes & Genomics Vol.43 No.12
Background Mitochondrial unfolded protein response plays an important role in the occurrence and development of breast cancer. However, the role of mitochondrial unfolded protein response (UPRmt) in the sensitivity of breast cancer to cisplatin chemotherapy has not yet been cleared. Objectives The purpose of this study is to explore the role of mitochondrial unfolded protein response in breast cancer sensitivity to cisplatin. Methods In this study, qRT-PCR, Western blotting, Immunofuorescence, CCK-8, Colony formation, Transwell assay and TUNEL staining assay were used to confrm the role of UPRmt in breast cancer cells treated with cisplatin. Results Cisplatin increased the levels of UPRmt including CLPP, HSP60, LONP1 in MCF7 and MDA-MB-231 cells. UPRmt inducer Nicotinamide ribose (NR) could promote the proliferation and invasion of breast cancer cells treated with cisplatin. Importantly, SIRT3 was discovered to increase UPRmt in breast cancer cells and silencing of SIRT3 could inhibit the efect of NR in breast cancer. Conclusions UPRmt regulated by SIRT3 could protect breast cancer cell from cisplatin. Controlling SIRT3-induced UPR may be a potential therapeutic target to increase the sensitivity of breast cancer chemotherapy.
Ying Yang,Dong Wang,Lei Cui,Hong-Hao Ma,Li Zhang,Hong-Yun Lian,Qing Zhang,Xiao-Xi Zhao,Li-Ping Zhang,Yun-Ze Zhao,Na Li,Tian-You Wang,Zhi-Gang Li,Rui Zhang 대한암학회 2021 Cancer Research and Treatment Vol.53 No.1
Purpose We sought to investigate the effectiveness and safety of dabrafenib in children with BRAFV600E-mutated Langerhans cell histiocytosis (LCH). Materials and Methods A retrospective analysis was performed on 20 children with BRAFV600E-mutated LCH who were treated with dabrafenib. Results The median age at which the patients started taking dabrafenib was 2.3 years old (range, 0.6 to 6.5 years). The ratio of boys to girls was 2.3:1. The median follow-up time was 30.8 months (range, 18.9 to 43.6 months). There were 14 patients (70%) in the risk organ (RO)+ group and six patients (30%) in the RO– group. All patients were initially treated with traditional chemotherapy and then shifted to targeted therapy due to poor control of LCH or intolerance to chemotherapy. The overall objective response rate and the overall disease control rate were 65% and 75%, respectively. During treatment, circulating levels of cell-free BRAFV600E (cfBRAFV600E) became negative in 60% of the patients within a median period of 3.0 months (range, 1.0 to 9.0 months). Grade 2 or 3 adverse effects occurred in five patients. Conclusion Some children with BRAFV600E-mutated LCH may benefit from monotherapy with dabrafenib, especially high-risk patients with concomitant hemophagocytic lymphohistiocytosis and intolerance to chemotherapy. The safety of dabrafenib is notable. A prospective study with a larger sample size is required to determine the optimal dosage and treatment duration.
Li Qin Zhang,Xiao Peng Zhang,Yu Ying Hao,Bao Li Zhang,Xin Dong Guo 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.88 No.-
When overexposed to ultraviolet B (UVB) irradiation, skin can cause a series of abnormal reactions, suchas excessive pigmentation and solar elastosis. In order to better treat the skin after overexposed to UVB,we designed a dissolving microneedles (MNs) loading hydrophilic ascorbic acid and hydrophobic vitaminA palmitate to achieve codelivery. Here, we confirmed that MNs had enough mechanical strength toinsert the skin and two drugs were delivered subcutaneously in sequence in vitro. In white guinea pigs,surface observation and histological observation results confirmed that two drugs by MNs transdermaldelivery were better than topical administration in the treatment of UVB-damaged skin. The MNs providea method of loading different drugs into the needles and advance the development of transdermal drugdelivery.
Jun Li,Liang Zhang,Xiao-Wen Zhang,Hao Zhang,Xue-Yin Jiang,Dong-bin Yu,Wen-Qing Zhu,Zhi-Lin Zhang 한국물리학회 2010 Current Applied Physics Vol.10 No.5
Bottom-contact (BC) copper phthalocyanine (CuPc) thin film transistor with UV/ozone treated Au as a source/drain electrode was fabricated and the contact resistance was estimated from the transmission line method (TLM). Comparing the properties of OTFT with untreated Au electrode, the performance of the BC CuPc-TFT with the UV/ozone treated Au electrodes was significantly improved: saturation mobility increased from 4.69 × 10−3 to 2.37 × 10−2 ㎠/V s, threshold voltage reduced from −29.1 to −6.4 V, and threshold swing varied from 5.08 to 2.25 V/decade. The contact resistance of the device with UV/ozone treated Au electrodes was nearly 20 times smaller than that of the device with untreated Au electrodes at the gate voltage of −20 V. This result indicated that using the UV/ozone treated Au electrode is an effective method to reduce the contact resistance. The present BC configuration with UV/ozone treated Au electrodes could be a significant step towards the commercialization of OTFT technology.
Survival Association and Cell Cycle Effects of B7H3 in Neuroblastoma
Zhang, Haibo,Zhang, Jinsen,Li, Chunjie,Xu, Hao,Dong, Rui,Chen, Clark C.,Hua, Wei The Korean Neurosurgical Society 2020 Journal of Korean neurosurgical society Vol.63 No.6
Objective : The function of B7H3, a member of the B7 family of proteins, in neuroblastoma (NB) remains poorly characterized. Here we examine the expression pattern of B7H3 in clinical NB specimens and characterize the phenotype of B7H3 knock-down in NB cell line. Methods : Immunohistochemical (IHC) staining was carried out to assess the expression of B7H3 in clinical NB specimens. Survival association was analyzed using five Gene Expression Omnibus (GEO) datasets (GSE85047, GSE45480, GSE62564, GSE16476, GSE49710). Clonogenic survival and flow cytometry were performed after B7H3 knockdown to assess the cellular proliferation and cell survival in vitro. Impact of B7H3 silencing on NB growth was examined in vivo using the SH-SY5Y xenograft model. Results : On IHC staining, B7H3 was widely expressed in clinical NB specimens. Analysis of the transcriptional profiles of five GEO datasets clinically annotated NB specimens revealed that decreased B7H3 expression was associated with improved overall survival. B7H3 knockdown suppressed the proliferation of the SH-SY5Y NB model in vitro and in vivo. Cell cycle analysis revealed that B7H3 silencing induced G1/S arrest. This arrest was associated with the suppression of E2F1 expression and induction of Rb expression. Conclusion : Our results demonstrate that B7H3 expression correlate with clinical survival in NB patients. Preliminary studies suggest that B7H3 may mediate the G1/S transition.
Serum Zinc Status and Helicobacter Pylori Infection in Gastric Disease Patients
Zhang, Wen-Hua,Wu, Xiao-Jing,Niu, Jing-Xiu,Yan, Hao,Wang, Xin-Zhuo,Yin, Xiao-Dong,Pang, Yan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10
The role of Helicobacter pylori status and serum zinc value in gastric disease patients and healthy controls were investigated. Cases used in this work were 45 gastric cancer patients, 44 with peptic ulcers, 52 suffering gastritis and 64 healthy controls, all diagnosed histologically with the controls undergoing medical checkups. Helicobacter pylori status and serum levels of Zn were determined by 13C-urea breath test and flame atomic absorption spectrophotometer, respectively. Our study showed that Helicobacter pylori infection has no change in gastritis, peptic ulcer and gastric cancer group, on the contrast, serum levels of Zn were significantly reduced in gastritis, peptic ulcer and gastric cancer group, compared with healthy controls, and the higher the Zn levels are, the more increased risk of gastric cancer. Helicobacter pylori infection is a cause of gastritis, peptic ulcers and even gastric cancer, while serum zinc level is an indicator of protection of gastric membranes against damage.