http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Zhi-gang Tai,Yi-ren Zhu,Yi-bo Yuan,Jin Liu,Zhen-jie Li,Zhi-hua Liu,Kun-miao Wang 대한화학회 2020 Bulletin of the Korean Chemical Society Vol.41 No.3
In this work, a highly sensitive method using a colorimetric probe coupled to dispersive liquid?liquid microextraction (DLLME) was developed for the quantitative determination of dopamine (DA) in serum. The DA in serum was concentrated by DLLME to increase the detection sensitivity and reduce the matrix effects. After the DLLME process, a colorimetric probe of silver triangular nanoparticles (AgTNPs) was used to detect DA, which was based on the plasma transformation of AgTNPs caused by strong interactions with melamine (MA). The results showed that DA could inhibit the aggregation of AgTNPs induced by MA, resulting in the recovery of the surface plasmon resonance (SPR) peak of AgTNPs. Thus, the DLLME method followed by colorimetric probe detection of DA can be achieved. The parameters affecting the proposed method were optimized, under the optimal conditions, a linear calibration curve was obtained over a concentration range of 5 to 250?nM with a recovery from 94.4 to 101.3%. The detection limit was 1.6 nM (at an S/N ratio of 3). The present method was successfully applied to determine DA in human serum.
Zhi-hao Li,Shang Chu,Ping Wang,Sihai Gao,Shu-gang Li,Xiongwei Yu 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.9
This study examined the effects of differentdoses of irradiation treatments on protein structure anddigestion characteristic of seed-watermelon seed kernelprotein. The results showed that, the molecular structure ofseed-watermelon kernel protein was unfolded after theirradiation treatment, the content of b-sheet structure in thesecondary structure was decreased, while the content ofrandom coil structure increased. The average particle sizeof the protein increased, and the hydrophobic group buriedin the b-sheet structure was exposed hence the surfacehydrophobicity increased. Besides, the surface morphologyof seed-watermelon protein changed from smooth and flatto coarse and concave, the specific surface area in contactwith the aqueous medium increased and its solubilityincreased, the distribution of peptides in the digestabecame wider, and the small molecular weight peptidesgradually increased.
Zhi-hao Li,Bin Zhou,Xiu-ting Li,Shu-gang Li 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.1
In this current study, comparative study betweenthe effect of electrolyzed water and ultrapure water on theextraction of apricot protein was conducted. The resultsrevealed that under the condition of same pH (pH = 9.5),the extraction efficiency of electrolyzed water on apricotprotein was superior to that of ultrapure water. Moreover,apricot protein (EAP) extracted by electrolyzed water displayedpreferable foaming capacity and emulsion stability. The foaming capacity and emulsion stability of EAP were11.17% and 36.33 min, for UAP, only 4.75% and23.88 min, respectively. Meanwhile, compared to UAP,the secondary structure of EAP was more orderly, in whichthe orderly structures of a-helix and b-sheet were 7.5 and60.2%, while the disorderly structures of b-turn and randomcoil were 8.4 and 23.8%. This work provided a novelextraction strategy, which could improve the extractionrate and minimize the destruction of the structure andfunctional properties of apricot protein.
Molecular cloning of two novel stearoyl-acyl desaturase genes from winterness wheat
Zhi-Fu Guo,Feng-Zhen Li,Xiao-Gang Ma,Feng Lin,Hui Ma,Li-Jing Chen,Ming Zhong,Li-Ping Bai,Ying Yi 한국유전학회 2011 Genes & Genomics Vol.33 No.5
Using RT-PCR and rapid amplification of cDNA ends, two new full-length cDNAs of SAD (TaSAD1 and TaSAD2) were obtained from a hardiest winter wheat cultivar (Mironovskaya808). Sequence comparison analysis showed that the deduced amino acid sequences of TaSAD1 and TaSAD2 had high similarity to those of other reported SAD proteins. They were also different each other by some substitutions, insertions and/or deletions involving single amino acid residues or motifs. Based on evolution analysis, it was clear that all SAD genes from Poaceae were closer than those from other genus such as Arabidopsis,Glycine, Triadica, Brassica, Sesamum and Bassia. All SAD genes clustered into two major groups in Poaceae. Meanwhile,TaSAD1 and TaSAD2 were clustered into different groups. The tertiary structure prediction indicated that both TaSAD1 and TaSAD2 proteins were a compact globular protein and their model structures almost were the same.
Adiponectin Receptor 1 (ADIPOR1) rs1342387 Polymorphism and Risk of Cancer: a Meta-analysis
Yu, Li-Xiang,Zhou, Nan-Nan,Liu, Li-Yuan,Wang, Fei,Ma, Zhong-Bing,Li, Jie,Yu, Zhi-Gang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18
Many studies have indicated possible associations between a polymorphism of adiponectin receptor 1 (ADIPOR1) rs1342387 and risk of cancer, but contradictory results have been reported. The main aim of this study was to draw a reliable conclusion about the relationship between the rs1342387 polymorphism and cancer incidence, by conducting a literature search of Pubmed, Embase, Wanfang and Cochrane libraries. Eleven studies including 3, 738 cases and 4, 748 controls were identified in this meta-analysis. The ADIPOR1 rs1342387 polymorphism was associated with risk of colorectal cancer for all genetic comparison models (GG vs AA, OR: 1.44, 95%CI: 1.21-1.70; G carriers vs A carriers, OR: 1.23, 95%CI: 1.11-1.36; dominant model, OR: 1.28, 95%CI: 1.10-1.49 and recessive model, OR: 1.31, 95%CI: 1.12-1.55). Stratified by ethnicity, the rs1342387 polymorphism was significantly associated with risk of colorectal cancer in Asian ancestry for all genetic comparison models (GG vs AA, OR: 1.56, 95%CI: 1.26-1.92; G carriers vs. A carriers OR: 1.30, 95%CI: 1.18-1.43; dominant model OR: 1.31, 95%CI: 1.08-1.60 and recessive model OR: 1.44, 95%CI: 1.26-1.64), but not in Caucasian or mixed (Caucasian mainly) groups. In summary, the ADIPOR1 rs1342387 polymorphism is significantly associated with risk of colorectal cancer among individuals of Asian ancestry.
Ran An,Shuai Wen,Dong-Li Li,Qiu-Hua Li,Xing-Fei Lai,Wen-Ji Zhang,Ruo-Hong Chen,Jun-Xi Cao,Zhi-Gang Li,Qiu-Sheng Huang,Ling-Li Sun,Shi-Li Sun 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.9
Tea and citrus maxima are natural, medicinal homologous plants, typically used for making beverages, which have anticancer, antiobesity, and antioxidation properties. Green tea, yellow tea, and black tea were combined with citrus maxima to obtain green tea and Citrus maxima (GTCM), yellow tea and Citrus maxima (YTCM), and black tea and Citrus maxima (BTCM). The biochemical components of these mixtures were analyzed, and their possible effects and mechanisms on relieving liver lipid deposition were explored. The tea polyphenols, free amino acids, phenolamine ratio, and caffeine were comparable in YTCM and GTCM, being significantly higher than those in BTCM. In addition, the content of esterified catechins, nonesterified catechins, and total catechins in YTCM was significantly higher than those in GTCM and BTCM. All three mixtures of Citrus maxima tea significantly reduced lipid deposition in HepG2 cells, with GTCM and YTCM being slightly more effective than BTCM. Regarding the possible mechanism, Western blot analysis revealed that the three Citrus maxima tea mixtures could activate the AMPK/ACC signaling pathway, upregulate the expression of p-AMPK, p-ACC, and CPT-1 proteins, and downregulate the expression of SREBP1c and fatty acid synthase proteins to inhibit fat synthesis, thereby relieving lipid deposition in liver cells. In conclusion, as a novel and healthy beverage, Citrus maxima tea has the potential to alleviate liver lipid deposition, and further could be responsible for obesity treatment.
Ying Yang,Dong Wang,Lei Cui,Hong-Hao Ma,Li Zhang,Hong-Yun Lian,Qing Zhang,Xiao-Xi Zhao,Li-Ping Zhang,Yun-Ze Zhao,Na Li,Tian-You Wang,Zhi-Gang Li,Rui Zhang 대한암학회 2021 Cancer Research and Treatment Vol.53 No.1
Purpose We sought to investigate the effectiveness and safety of dabrafenib in children with BRAFV600E-mutated Langerhans cell histiocytosis (LCH). Materials and Methods A retrospective analysis was performed on 20 children with BRAFV600E-mutated LCH who were treated with dabrafenib. Results The median age at which the patients started taking dabrafenib was 2.3 years old (range, 0.6 to 6.5 years). The ratio of boys to girls was 2.3:1. The median follow-up time was 30.8 months (range, 18.9 to 43.6 months). There were 14 patients (70%) in the risk organ (RO)+ group and six patients (30%) in the RO– group. All patients were initially treated with traditional chemotherapy and then shifted to targeted therapy due to poor control of LCH or intolerance to chemotherapy. The overall objective response rate and the overall disease control rate were 65% and 75%, respectively. During treatment, circulating levels of cell-free BRAFV600E (cfBRAFV600E) became negative in 60% of the patients within a median period of 3.0 months (range, 1.0 to 9.0 months). Grade 2 or 3 adverse effects occurred in five patients. Conclusion Some children with BRAFV600E-mutated LCH may benefit from monotherapy with dabrafenib, especially high-risk patients with concomitant hemophagocytic lymphohistiocytosis and intolerance to chemotherapy. The safety of dabrafenib is notable. A prospective study with a larger sample size is required to determine the optimal dosage and treatment duration.
A guided bone regeneration membrane composed of hydroxyapatite and polyurethane
Zhi-Hong Dong,Li Zhang,Gang Zhou,이수완,Yu-Bao Li 한양대학교 세라믹연구소 2008 Journal of Ceramic Processing Research Vol.9 No.5
A hydroxyapatite (HA,10 wt%) and polyurethane (PU) composite as a guided bone regeneration (GBR)membrane was obtained from the polycondensation or polyaddition of diisocyanates and hydroxyl groups by a solvent evaporation method. The structure and properties of the membrane were investigated by XRD, IR, SEM, water absorption, wettability and a cell culture test in vitro. The results show that hydroxyapatite particles were dispersed uniformly in the polyurethane matrix and interfacial bonding between hydroxyapatite and polyurethane was formed. The hydroxyapatite/polyurethane membrane has good hydrophilicity and the surface pores can promote cell adhesion and growth. MTT (3-{4,5-dimethylthiazol-2yl}-2,5-diphenyl-2H-tetrazolium bromide) assay and light microscopy observation indicate that the hydroxyapatite/polyurethane membrane demonstrates excellent biocompatibility. The hydroxyapatite/polyurethane membrane will hopefully be selected as a guided bone regeneration and tissue engineering.