콘택트렌즈 보존 용기 유래 Acanthamoeba lugdunensis KA/L5주의 내공생세균

정동일,공현희,김태호,황미열,유학선,윤호철,설성용 경북대학교 의학연구소 1999 경북대학교병원의학연구소논문집 Vol.3 No.1

콘택트렌즈 보존 용기 유래 가시아메바 KA/L5주의 세포질 내에 존재하는 bacterial endosymbiont(내공생세균)를 투과전자현미경으로 관찰하여 확인하였다. 숙주인 가시아메바 KA/L5주는 형태학적으로 제2군에 속하였고, rDNA PCR-RFLP 결과 A. lugdunensis로 동정되었다. 미토콘드리아 DNA RFLP와 동위효소 분석상 이 충주는 국내 콘택트렌즈 보존용기에서 가장흔히 분리되는 type인 KA/Ll주, 국내 임상 분리주 중 하나인 KA/E2주, 내공생세균을 가지는 것으로 보고된 병원 냉각수 유래 KA/W4주 및 L3a주와 동일하거나 매우 유사한 성적을 보였다. 내공생세균은 약 1.38× 0.507㎛의 크기였고, 아메바 세포질 내에 불규칙하게 분포하고 있었으며. 그 표면에 아메바의 ribosome이 부착되어 있었다. 내공생세균을 둘러싼 lacunae나 막과 같은 구조는 관찰되지 않았다. Legionella 특이 primer를 이용한 효소중합반응(PCR)에서 내공생세균의 염색체 DNA는 증폭되지 않았다. A. lugdunensis의 우리말 이름을 담수가시아메바로 제안한다. Transmission electron microscopy of an Acanthamieba isolate (KA/L5) from a contact lens case revealed bacterial endosymbionts within cytoplasm of the amoebae, The Acanthamoeba isolate belonged to the morphological group II. Based on the polymerase chain reaction (PCR) - restriction fragment length Polymorphism (RFLP) of 18S ribosomal RNA coding DNA (rDNA), the Isolate was identified as A. lugdunensis. Strain typing by isoenzyme analysis using isoelectric focusing (IEF) and mitochondrial (Mt) DNA RFLP revealed that the isolate was closely related with KA/Ll , the most predominant type of isolates from contact lens storage cases, KA/E2, a clinical isolate, KA/W4, preciously reported to host endosymbionts, and L3a strains of A. lugdunensis. The endosymbionts were similar to those of KA/W4 In aspects that they were randomly distributed in both trophozoites and cysts, and were rod-shaped bacteria measuring approximately 1.38x 0.50㎛. But the number of endosymbionts per amoeba was significantly lower than that of KA/W4. They were neither limited by phagosomal membranes nor included In lacunae- like structure.

Reconsidering the Hierarchy and Translation of “Physical Education/Sports” Related Terms: Taiwan Perspective

Jeffrey Yu,Jun Lian,Ruei-Hong Li,Chen-Sin Hung,Dong-Tai Chen,Yu-Kai Chang 대한운동학회 2024 아시아 운동학 학술지 Vol.26 No.1

Objectives In the past centuries, the concept of “physical education” has changed from a more general concepts including physical activities and sports, to specifically inferring “instruction of physical activity”. Instead, “sports” has become the comprehensive concept of sports, exercise, and physical education. Given to the inconsistent terminologies and English translations of “體育 (ti-yu)” (physical education) and “運 動 (yun-dong)” (sports or exercise) in Mandarin, the purpose of this study was to investigate in the current terminology usage, hierarchy, and the English translation of “體育 (ti-yu)” and “運動 (yun-dong)” in Taiwan. Met hods Three government authorities, two sport related universities, eight top sport and exercise journals, and nighty-six college departments in Taiwan were analyzed. Results “運動 (yun-dong)” was the most widely used terminology as “體育 (ti-yu)” occupied the majority usage among government authorities, sport related universities, and title of top sport and exercise journals. Regarding English translation, despite of “department of physical education”, “sports” remained the most common terminology. Moreover, “體育 (ti-yu)” and “sports” are the highest hierarchy among government authorities, and sport related university; “體育 (ti-yu)” and “physical education” are the highest hierarchy among traditional sport and exercise journals; “運動 (yun-dong)” and “sports” are the highest hierarchy among college departments also the mainstream of current translation and hierarchy. Conclusions “體育 (ti-yu)” was the highest hierarchy in the past. However, “運動 (yun-dong)” has been the mainstream of the highest hierarchy in Mandarin according to college departments. In English, “sport(s)” is the main term in Taiwan when translating “體育 (ti-yu)” and 運動 (yun-dong)”, also being the highest hierarchy. On the other hand, “體育 (ti-yu)” and “physical education” are utilized regarding those departments focusing on educating PE teachers. This study expects the terminology, English translations, and hierarchy being align with the mainstream of current translation and hierarchy in the future.

Effect of inorganic mesoporous carriers on 1-palmitoyl-2-linoleoyl-3-acetyl-<i>rac</i>-glycerol-loaded solid self-emulsifying drug delivery system: Physicochemical characterization and bioavailability in rats

Kim, Dong Shik,Yang, Eun Su,Yong, Chul Soon,Youn, Yu Seok,Oh, Kyung Taek,Li, Dong Xun,Kim, Jong Oh,Jin, Sung Giu,Choi, Han-Gon Elsevier 2017 Colloids and Surfaces B Vol.160 No.-

<P><B>Abstract</B></P> <P>The purpose of this study was to assess the impact of inorganic mesoporous carriers on the physicochemical properties and oral bioavailability of 1-palmitoyl-2-linoleoyl-3-acetyl-<I>rac</I>-glycerol (PLAG)-loaded solid self-emulsifying drug delivery system (solid SEDDS). Numerous PLAG-loaded solid SEDDS formulations were prepared by spray drying technique with sodium laurylsulfate (SLS), butylated hydroxyanisole (BHA) and inorganic mesoporous materials as a surfactant, antioxidant and solid carrier, respectively. The mesoporous materials, such as calcium silicate, silicon dioxide and magnesium aluminosilicate were used as the solid carriers. Their physicochemical properties, solubility, dissolution and pharmacokinetic studies in rats were performed compared with drug alone. Three solid SEDDSs composed of PLAG/BHA/SLS/mesopous carrier at the weight ratio of 1:0.0002:0.25:0.5 resulted in a small emulsion droplet and excellent drug loading efficiency. The solid SEDDS formulations prepared with calcium silicate and silicon dioxide showed a rough-surfaced irregular shape and rough-surfaced spheres, respectively. Magnesium aluminosilicate generated a sticky powder, due to its relatively low specific surface area, resulting in insufficient adsorption of PLAG. These solid SEDDSs improved the solubility, dissolution and oral bioavailability of PLAG. Ultimately, the solid SEDDS prepared with silicon dioxide resulted in the best drug loading efficiency, shape, solubility, dissolution and oral bioavailability due to its great specific surface area. Therefore, mesoporous carriers with different specific surface areas markedly influenced the physicochemical properties, solubility, dissolution and oral bioavailability of PLAG-loaded solid SEDDS.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The effect of mesoporous carriers on PLGA-loaded solid SEDDS were assessed. </LI> <LI> Numerous PLGA-loaded solid SNEDDS were prepared using spray drying technique. </LI> <LI> Calcium silicate, silicon dioxide and magnesium aluminosilicate were used as the mesoporous carriers. </LI> <LI> The solid SEDDS prepared with silicon dioxide gave most excellent loading efficiency and bioavailability. </LI> <LI> Mesoporous carriers markedly influenced the physicochemical properties and bioavailability of solid SEDDS. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

SERS study of surface plasmon resonance induced carrier movement in Au@Cu₂O core-shell nanoparticles

Chen, Lei,Zhang, Fan,Deng, Xin-Yu,Xue, Xiangxin,Wang, Li,Sun, Yantao,Feng, Jing-Dong,Zhang, Yongjun,Wang, Yaxin,Jung, Young Mee Elsevier 2018 Spectrochimica acta. Part A, Molecular and biomole Vol.189 No.-

<P><B>Abstract</B></P> <P>A plasmon induced carrier movement enhanced mechanism of surface-enhanced Raman scattering (SERS) was investigated using a charge-transfer (CT) enhancement mechanism. Here, we designed a strategy to study SERS in Au@Cu<SUB>2</SUB>O nanoshell nanoparticles with different shell thicknesses. Among the plasmonically coupled nanostructures, Au spheres with Cu<SUB>2</SUB>O shells have been of special interest due to their ultrastrong electromagnetic fields and controllable carrier transfer properties, which are useful for SERS. Au@Cu<SUB>2</SUB>O nanoshell nanoparticles (NPs) with shell thicknesses of 48–56nm are synthesized that exhibit high SERS activity. This high activity originates from plasmonic-induced carrier transfer from Au@Cu<SUB>2</SUB>O to 4-mercaptobenzoic acid (MBA). The CT transition from the valence band (VB) of Cu<SUB>2</SUB>O to the second excited π-π* transition of MBA, and is of b<SUB>2</SUB> electronic symmetry, which was enhanced significantly. The Herzberg-Teller selection rules were employed to predict the observed enhanced b<SUB>2</SUB> symmetry modes. The system constructed in this study combines the long-range electromagnetic effect of Au NPs, localized surface plasmon resonance (LSPR) of the Au@Cu<SUB>2</SUB>O nanoshell, and the CT contribution to assist in understanding the SERS mechanism based on LSPR-induced carrier movement in metal/semiconductor nanocomposites.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We designed a shell-dependent Au@Cu<SUB>2</SUB>O nanoshell for SERS study. </LI> <LI> SERS contribution enables us to understand the possible enhancement of hybrid nanostructures. </LI> <LI> LSPR-induced carrier movement in Au@Cu<SUB>2</SUB>O nanocomposites. </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>We designed the shell-dependent Au@Cu<SUB>2</SUB>O core-shell nanoparticles (NPs) for SERS study. For the electron-hole pairs in the Cu<SUB>2</SUB>O, the plasmon induced resonant energy transfer from Au to the Cu<SUB>2</SUB>O and the direct electron transfer simultaneously which can be observed from the SERS intensity.</P> <P>[DISPLAY OMISSION]</P>

Purification and Characterization of Cathepsin B from the Gut of the Sea Cucumber (Stichopus japonicas)

Li-Ming Sun,Bei-Wei Zhu,Hai-tao Wu,Lei Yu,Da-Yong Zhou,Xiuping Dong,Jing-Feng Yang,Dong-Mei Li,Wen-Xiu Ye,Yoshiyuki Murata 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.4

Cathepsin B from the gut of sea cucumber (Stichopus japonicas) was purified 81-fold with a 3%recovery by ammonium sulfate fractionation and a series chromatography on DEAE Sepharose CL-6B, Sephadex G-75, and TSK-Gel 3000 SWxl. The purified protein appeared as a single band on Native-PAGE but showed 2bands of 23 and 26 kDa on SDS-PAGE. The optimum activity was found at pH 5.5 and 45°C. The enzyme was stable at pH 4.5-6.0 and the thermal stability was up to 50oC. The enzyme was strongly inhibited by E-64, iodoacetic acid, and antipain, demonstrating it is a cysteine protease containing sulfhydryl groups. Cu^2+, Ni^2+, and Zn^2+ could strongly inhibit the enzyme activity. The amino acid sequences of the purified enzyme were acquired by mass spectrometer, which did not show any homology with previously described cathepsins, suggesting it may be a novel member.

Staged Improvement in Awareness of Disease for Elderly Cancer Patients in Southern China

Li, Xing,Dong, Min,Wen, Jing-Yun,Wei, Li,Ma, Xiao-Kun,Xing, Yan-Fang,Deng, Yun,Chen, Zhan-Hong,Chen, Jie,Ruan, Dan-Yun,Lin, Ze-Xiao,Wang, Tian-Tian,Wu, Dong-Hao,Liu, Xu,Hu, Hai-Tao,Lin, Jia-Yu,Li, Zhu Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.15

Background: In mainland China, awareness of disease of elderly cancer patients largely relies on the patients' families. We developed a staged procedure to improve their awareness of disease. Materials and Methods: Participants were 224 elderly cancer patients from 9 leading hospitals across Southern China. A questionnaire was given to the oncologists in charge of each patient to evaluate the interaction between family and patients, patient awareness of their disease and participation in medical decision-making. After first cycles of treatment, increased information of disease was given to patients with cooperation of the family. Then patient awareness of their disease and participation in medical decision-making was documented. Results: Among the 224 cancer elderly patients, 26 (11.6%) made decisions by themselves and 125 (55.8%) delegated their rights of decision-making to their family. Subordinate family members tended to play a passive role in decision-making significantly. Patients participating more in medical decision-making tended to know more about their disease. However, in contrast to the awareness of disease, patient awareness of violation of medical recommendations was reversely associated with their participation in medical decision-making. Improvement in awareness of diagnosis, stages and prognosis was achieved in about 20% elderly cancer patients. About 5% participated more actively in medical decision-making. Conclusions: Chinese elderly cancer patient awareness of disease and participation in medical decision-making is limited and relies on their family status. The staged procedure we developed to improve patient awareness of disease proved effective.

A Wearable Pressure Sensor Based on Ionogel/Textile for Human Motion Monitoring

Yu Dong,Qiujin Li,Zhiqi Zhao,Jixian Gong,Zheng Li,Jianfei Zhang 한국섬유공학회 2022 Fibers and polymers Vol.23 No.8

The growth of flexible sensors has always been challenging since the sensing behavior, mechanic performance andfatigue resistance remain to be improved. In this paper, a wearable pressure sensor with fast response and high sensitivitybased on ionogel/textile is fabricated. The ionogel is a double-network (DN) structure composed of polydimethylacrylamide(PDMAAm) skeleton and silica particles, carrying ionic liquids (ILs), which serves as a piezoresistive device. Byimpregnating and polymerization with the ionogel-prepolymer solution, a conventional cotton knitted fabric is converted intoa conductive and piezoresistive composite. The fabric serves as a ductile supporting matrix for the ionogel, supplementing thepiezoresistive contribution of the brittle silica network. This ionogel/textile composite has a high sensitivity (S=86.90 kPa-1)and durability, continuously outputting stable electric signal during monitoring the motion states of different joints in humanbody. Through the impregnation-polymerization approach, the selected areas toward to the specific joints positions inclothing can be fabricated into piezoresistive devices. The incorporation of textile improves the stress loading and sensingbehavior of the ionogel to work steadily under the sole of the foot for gait monitoring. This ionogel/textile device provides apotential formula of wearable sensors, stepping toward the burgeoning diversified-technologies assisted intelligence system.

Enhanced Solubility and Bioavailability of Sibutramine Base by Solid Dispersion System with Aqueous Medium

Li, Dong Xun,Jang, Ki-Young,Kang, Wonku,Bae, Kyoungjin,Lee, Mann Hyung,Oh, Yu-Kyoung,Jee, Jun-Pil,Park, Young-Joon,Oh, Dong Hoon,Seo, Youn Gee,Kim, Young Ran,Kim, Jong Oh,Woo, Jong Soo,Yong, Chul Soon Pharmaceutical Society of Japan 2010 Biological & pharmaceutical bulletin Vol.33 No.2

<P>To develop a novel sibutramine base-loaded solid dispersion with improved solubility bioavailability, various solid dispersions were prepared with water, hydroxypropylmethyl cellulose (HPMC), poloxamer and citric acid using spray-drying technique. The effect of HPMC, poloxamer and citric acid on the aqueous solubility of sibutramine was investigated. The physicochemical properties of solid dispersion were investigated using scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and X-ray powder diffraction. The dissolution and pharmacokinetics in rats of solid dispersion were evaluated compared to the sibutramine hydrochloride monohydrate-loaded commercial product (Reductil<SUP>®</SUP>). The sibutramine base-loaded solid dispersion gave two type forms. Like conventional solid dispersion system, one type appeared as a spherical shape with smooth surface, as the carriers and drug with relatively low melting point were soluble in water and formed it. The other appeared as an irregular form with relatively rough surface. Unlike conventional solid dispersion system, this type changed no crystalline form of drug. Our results suggested that this type was formed by attaching hydrophilic carriers to the surface of drug without crystal change, resulting from changing the hydrophobic drug to hydrophilic form. The sibutramine-loaded solid dispersion at the weight ratio of sibutramine base/HPMC/poloxamer/citric acid of 5/3/3/0.2 gave the maximum drug solubility of about 3 mg/ml. Furthermore, it showed the similar plasma concentration, area under the curve (<I>AUC</I>) and <I>C</I><SUB>max</SUB> of parent drug, metabolite I and II to the commercial product, indicating that it might give the similar drug efficacy compared to the sibutramine hydrochloride monohydrate-loaded commercial product in rats. Thus, this solid dispersion system would be useful to deliver poorly water-soluble sibutramine base with enhanced bioavailability.</P>

Study on the Effects of Pedestrians on the Aerostatic Response of a Long-Span Pedestrian Suspension Bridge

Yu Li,Zhe Chen,Shi-Jie Dong,Jia-Wu Li 대한토목학회 2021 KSCE Journal of Civil Engineering Vol.25 No.10

According to a long-span pedestrian suspension bridge, the sectional models of the human-footbridge system were proposed, and then the force measurement tests were performed to study the effects of the pedestrian density (ρ), pedestrian permutation, and wind attack angle (α) on the drag force coefficient (CH), lift force coefficient (CV), and pitching moment coefficient (CM), respectively. So, it is found that: (1) with the increase of ρ, both CH and CV with α < -2o increase, while both CV with α ≥ -2o and CM with ρ ≠ 0.0 P/m2 decrease. (2) when ρ> 0.2 P/m2, there is a significant effect of the pedestrian permutations on CH and CM, while the effect of the pedestrian permutations on CV is small. Then, the static wind forces were calculated and applied to the pedestrian suspension bridge, and the effects of ρ and pedestrian permutations on the aerostatic response were studied. So, it is found that: 1) with the increase of ρ, the aerostatic response increases; 2) the effects of the pedestrian permutations with ρ ≤ 0.5 P/m2 on the aerostatic response can be ignored, while there are disadvantageous effects of the pedestrian permutations with ρ > 0.5 P/m2 and larger wind-blocking area on the aerostatic response.

Terpenoid composition and the anticancer activity of Acanthopanax trifoliatus

Dong-Li Li,Xi Zheng,Yu-Chan Chen,Sen Jiang,Wei-Min Zhang,Huaqian Wang,Zhi-Yun Du,Kun Zhang 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.1

The petroleum ether and ethyl acetate fractions of extract from an edible and medicinal plant Acanthopanax trifoliatus were found to show significant inhibitory effects against SF-268, MCF-7, HepG2 and NCIH460 cancer cells. Two new ursane-type triterpenoids, acantrifoic acid C (1) and acantrifoic acid D (2), along with five known triterpenoids (3–7) and eight known diterpenoids (8–15) were obtained from these two fractions. To the best of our knowledge, this is the first report concerning the isolation of compounds (5–12, 14, 15) from A. trifoliatus. Among all the isolated compounds, 3, 5 and 8 from the ethyl acetate fraction showed the strongest inhibitory effects against cancer cells, while 12 and 13 from the petroleum ether fraction showed moderate activities. These terpenoid compounds may be responsible for the anticancer activities of A. trifoliatus. Our study provides the first evidence that terpenoids from A. trifoliatus exert anticancer activities and indicates that A. trifoliatus may be a useful edible plant for further development of anticancer health supplement.