Dynamics of oppositely charged carriers in a metal/coupled poly(p-phenylene vinylene) chains/metal structure

Dong-Mei Li,Yuan Li,Hai-Hong Li,Desheng Liu 한국물리학회 2011 Current Applied Physics Vol.11 No.2

By using the extended version of the Su-Schrieffer-Heeger (SSH) tight-binding model and a non-adiabatic dynamic evolution method, we present a study of the dynamic process of oppositely charged carriers in a metal/two coupled poly(p-phenylene vinylene) chains/metal structure. Electrons and holes can be injected into the polymer chains from metal electrodes by applying voltage biases. The behavior of the injected charged carriers depends closely on the electric field and the interchain coupling. The moderate intensity of electric field is in favor of forming stable wave packets, and the charge carriers localized in wave packets occur to transport, hopping, collide and recombine. With increasing the electric field, part of the carriers can be ejected into the metal electrodes.

Intermedins A and B; New Metabolites from Schisandra propinqua var. intermedia

Li, Hong-Mei,Lei, Chun,Luo, Yong-Ming,Li, Xiao-Nian,Li, Xiao-Lei,Pu, Jian-Xin,Zhou, San-Yun,Li, Rong-Tao,Sun, Han-Dong 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.6

A new dibenzocyclooctadiene lignan, intermedin A (1), and a new natural bisabolane sesquiterpenoid, intermedin B (2), were isolated from the aerial parts of Schisandra propinqua var. intermedia. Their structures were elucidated on the basis of extensive spectroscopical analysis.

KLF4 suppresses the tumor activity of cutaneous squamous cell carcinoma (SCC) cells via the regulation of SMAD signaling and SOX2 expression

Li, Xue Mei,Kim, Soo Jung,Hong, Dong-Kyun,Jung, Kyoung Eun,Choi, Chong Won,Seo, Young-Joon,Lee, Jeung-Hoon,Lee, Young,Kim, Chang-Deok Elsevier 2019 Biochemical and biophysical research communication Vol.516 No.4

<P><B>Abstract</B></P> <P>Kruppel-like factor 4 (KLF4) is a zinc-finger transcription factor that plays a role in terminal differentiation of epidermal keratinocytes. There are conflicting reports regarding the role of KLF4 in tumor development, with both the tumor suppressive and/or oncogenic properties depending on different conditions and cell types. In this study, we investigated the functional importance of KLF4 in cutaneous squamous cell carcinoma (SCC). Immunohistochemistry showed that KLF4 expression was relatively low in SCC lesion compared to normal epidermis. To examine the effects of KFL4, we transduced SCC lines (SCC12 and SCC13 cells) with the KLF4-expressing recombinant adenovirus. Overexpression of KLF4 significantly decreased cell proliferation and colony forming activity. In addition, overexpression of KLF4 markedly reduced invasive potential, along with the downregulation of epithelial-mesenchymal transition (EMT)-related molecules. In a mechanistic study, KLF4 inhibited SOX2, of which expression is critical for tumor initiation and growth of SCC. Further investigations indicated that SOX2 expression is induced by TGF-β/SMAD signaling, and that overexpression of KLF4 inhibited SMAD signaling via upregulation of SMAD7, an important inhibitory SMAD molecule. Based on these data, KLF4 plays a tumor suppressive role in cutaneous SCC cells.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Overexpression of KLF4 decreased the cell proliferation, colony forming activity, invasion and EMT potential in cutaneous SCC cells. </LI> <LI> Overexpression of KLF4 decreased SOX2 level via inhibition of SMAD2 phosphorylation. </LI> <LI> Overexpression of KLF4 inhibited SMAD signaling via upregualtion of SMAD7, an inhibitory SMAD molecule. </LI> </UL> </P>

Prospective comparison of hybrid capture 2 and SPF10-LiPA for carcinogenic human papillomavirus detection and risk prediction of cervical cancer: a population-based cohort study in China

Li Dong,Rui-Mei Feng,Li Zhang,Xiao-qian Xu,Xue-Lian Zhao,Margaret Zhuoer Wang,You-Lin Qiao,Fang-Hui Zhao 대한부인종양학회 2017 Journal of Gynecologic Oncology Vol.28 No.5

Objective: To investigate the extent of the cross-reactivity of hybrid capture 2 (HC2) assay andevaluate the potential effect of cross-reactivity on the long-term risk for cervical cancer andprecancers. Methods: Based on the Shanxi Province Cervical Cancer Screening Study-I (SPOCCS-I)cohort from 2005 to 2014 in Shanxi, China, SPF10-line probe assay (LiPA) was performedin all 598 HC2 positive and 300 random-selected HC2 negative cervical specimens. Tenyearcumulative incidence rate (CIR) of cervical intraepithelial neoplasia grade 2 or worse(CIN2+) of these two tests was evaluated using Kaplan-Meier methods. Possible humanpapillomavirus (HPV) types to be cross-reacted by HC2 were also analyzed. Results: The overall agreement between HC2 and SPF10-LiPA for detecting carcinogenic HPVwas 73.27%. The highest 10-year cumulative risk of CIN2+ was observed in both HC2 positiveand LiPA-carcinogenic HPV positive women (25.70%; 95% confidence interval [CI]=23.55%–27.91%), followed by HC2 positive but LiPA-non-carcinogenic HPV positive women (9.97%;95% CI=8.57%–11.50%), HC2 negative but LiPA-carcinogenic HPV positive (2.56%; 95%CI=2.44%–2.70%) and HC2 positive but LiPA-HPV negative (1.85%; 95% CI=1.78%–1.92%)women. The proportion of cross-reactivity of HC2 with untargeted carcinogenic types was8.9%, most of which were attributable to HPV26, 73, 82, 69, 71, 53, 11, 43, and 54. Conclusion: The noticeable high risk of CIN2+ in women infected with cross-reacted noncarcinogenicHPV and low risk in those with miss-to-detective carcinogenic HPV supportedan overall good clinical performance of HC2 for a general cervical cancer screening.

Antiviral activity of Herba Patrinea (a Chinese medicinal herb) against respiratory syncytial virus in vitro

Li, Hong-Yuan,Li, Shan-Shan,Liu, Dian-Li,Dong, Yan-Mei,Tian, Wen-Jing Kyung Hee Oriental Medicine Research Center 2003 Oriental pharmacy and experimental medicine Vol.3 No.2

Respiratory syncytial virus (RSV) has long been considered an important cause of severe lower respiratory tract infection in infants and young children throughout the world. Unfortunately, no effective treatment of RSV exists. Therefore, New agents are needed to reduce the impact of RSV. We have studied the anti-viral effect of traditional Chinese midicinal herbs for over ten years and find Herba Patrinea (a Chinese medicinal herb) has the anti-RSV effect in vitro. In this study, the Herba Patrinea was extracted with hot water, condensed and sterilized. The cytotoxicity of the aqueous extract was tested by adding the diluted extract directly to HeLa cells and its effect on anti-RSV was estimated by the CPEI assay. As a result, the median cytotoxic concentration $(CC_{50})$ of Herba Patrinea was 32 mg/ ml by morphological observation, the median effective concentration (50% effective concentration, $EC_{50}$) of the Herba Patrinea against replication of the Long strain of RSV in HeLa cells were 1.25 mg/ml. The selectivity index $(SI=CC_{50}/EC{50})$ is 25.6. Moreover, Herba Patrinea gave a dose-dependent response in inhibiting RSV. In time of addition experiment, Herba Patrinea inhibited replication of RSV in HeLa cells when it was added at 0h, 2h, and 4h after virus infection. In summary, the results of this study suggest Herba Patrinea may be a novel anti-RSV drug and it is worthy of further studying.

ent-Abietane Diterpenoids from Isodon xerophilus

Li Mei Li,Jian Xin Pu,Wei Lie Xiao,Han-Dong Sun 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.6

Three new ent-abietanoids, named xerophilusins XIV–XVI, and four known analogues, as well as four known chemical constituents were isolated from the leaves of Isodon xerophilus. Their structures were elucidated by extensive spectroscopic studies, and comparison with literature data. In addition, the cytotoxic activity of the ent-abietanoids against chronic myelogenous leukemia (K562), stomach adenocarcinoma (MKN45), and hepatocellular carcinoma (HepG2) human cell lines was investigated and no activities were observed.

Purification and Characterization of Cathepsin B from the Gut of the Sea Cucumber (Stichopus japonicas)

Li-Ming Sun,Bei-Wei Zhu,Hai-tao Wu,Lei Yu,Da-Yong Zhou,Xiuping Dong,Jing-Feng Yang,Dong-Mei Li,Wen-Xiu Ye,Yoshiyuki Murata 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.4

Cathepsin B from the gut of sea cucumber (Stichopus japonicas) was purified 81-fold with a 3%recovery by ammonium sulfate fractionation and a series chromatography on DEAE Sepharose CL-6B, Sephadex G-75, and TSK-Gel 3000 SWxl. The purified protein appeared as a single band on Native-PAGE but showed 2bands of 23 and 26 kDa on SDS-PAGE. The optimum activity was found at pH 5.5 and 45°C. The enzyme was stable at pH 4.5-6.0 and the thermal stability was up to 50oC. The enzyme was strongly inhibited by E-64, iodoacetic acid, and antipain, demonstrating it is a cysteine protease containing sulfhydryl groups. Cu^2+, Ni^2+, and Zn^2+ could strongly inhibit the enzyme activity. The amino acid sequences of the purified enzyme were acquired by mass spectrometer, which did not show any homology with previously described cathepsins, suggesting it may be a novel member.

Efficacy of high-voltage, long-duration pulsed radiofrequency for the treatment of acute herpes zoster-related trigeminal neuralgia in a patient with permanent pacemaker

Li-Mei Li,Xiao-Dong Liu,Yue Liu,Ting-ting Liu,Zhi-Li Zhang 대한통증학회 2024 The Korean Journal of Pain Vol.37 No.2

Intermedins A and B; New Metabolites from Schisandra propinqua var. intermedia

Hong-Mei Li,Chun Lei,Yong-Ming Luo,Xiao-Nian Li,Xiao-Lei Li,Jian-Xin Pu,San-Yun Zhou,Rong-Tao Li,Han-Dong Sun 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.6

A new dibenzocyclooctadiene lignan, intermedin A (1), and a new natural bisabolane sesquiterpenoid, intermedin B (2), were isolated from the aerial parts of Schisandra propinqua var. intermedia. Their structures were elucidated on the basis of extensive spectroscopical analysis.

암살의 유래 동아시아 아나키스트의 테러운동에 대하여

이동매 ( Li Dong-mei ) 민족문학사학회·민족문학사연구소 2018 민족문학사연구 Vol.68 No.-

20세기 초 암살은 아나키즘 운동의 중요한 수단으로 여겨졌다. 그러나 사실상 아나키즘과 암살의 결합은 ‘허무당’이라는 매개에 의한 것이었다. 1881년에 러시아 황제 알렉산더 2세가 ‘인민의 의지당’에 의해 암살당하였는데 이 사건이 동아시아로 전파되는 과정에서 일본 지식인의 오독으로 인하여 테러리스트는 허무당(Nihilist)으로 소개되었다. 러시아의 허무주의(Nihilism)는 1860년대에 성행하였으며 1970년대에는 나로드니키주의로 발전되었다. 나로드니키주의는 바쿠닌으로 대변되는 폭동파, 라브로프로 대표되는 선전파 그리고 트카초프로 대변되는 음모파 등 3개의 경향으로 나누어졌다. 바쿠닌의 폭동파는 전국적인 농민운동을 주장하였고 음모파는 개인적 암살이라는 수단을 선호하였다. 후에 음모파가 ‘인민의 의지당’으로 발전되었고 그들에 의해 알렉산더 2세는 암살당하였다. 바쿠닌의 폭동주장과 트카초프의 암살주장은 서로 달랐다. 즉 ‘인민의 의지당’의 테러운동은 허무주의를 온전히 대변할 수 없었던 것이다. 테러리스트를 허무당으로 소개한 것은 ‘인민의 의지당’의 테러주장을 허무주의로 확장시키는 오독을 야기했다. 1901∼1902년에 『社會主義槪評』과 『近世無政府主義』 두 권의 저작이 출간되었는데 이 저작들은 허무당이 알렉산더 2세를 암살하였다고 서술하면서 허무주의와 아나키즘을 같은 것이라고 주장하였다. 이는 바쿠닌의 이중적 신분으로 인해 발생한 오해라고 볼 수 있다. 바쿠닌은 허무주의자이자 대표적인 아나키스트이다. 때문에 20세기 초 일본 지식인들은 아나키즘과 허무주의를 혼동하였던 것으로 보인다. 그 결과로 아나키즘은 암살을 주장하는 사상으로 인식되게 되었다. Assassination was once considered an important means of anarchist movement in the early 20th century, but anarchism does not appear with assassination, the combination of anarchism and assassination went through a historical process, in which the concept of Nihilists played a very important role. In 1881, Tsar Alexander II was assassinated by Narodnaya Volya and this news was quickly spread to East Asia, but Narodnaya Volya was translated into Nihilists in this process. Additionally, due to the huge impact of the assassination of Alexander II, Nihilists was considered a party specializing in assassination. But Nihilists never appeared in Russia, the so-called Nihilists is a translation of Nihilism. Nihilism prevailed in 1860 in Russia and later became closely integrated with Populism. The Populist movement was divided into three schools : the Riots of Bakunin, the Conspiracy of Tkachev, and the Propaganda of Razrov. Among them, Bakunin advocated social change through nationwide peasant riots; Tkachov advocated overthrowing the feudal rule through personal assassination, which later developed into the creed of Narodnaya Volya and Narodnaya Volya also succeeded in assassinating Alexander II. Therefore, the assassination claim is only a diversion of Nihilism, which was different from Bakunin’s claim. Socialist Review and Modern Anarchism were published in Japan during 1901 to 1902 successively, these works argued that it was Nihilists that assassinated Alexander II and claimed that Nihilists and Anarchist are the same party. There are two problems here : First, it described the assassin of Alexander II thanks to Nihilists, actually it’s stealing the concept ― it extended the assassination of Narodnaya Volya to the whole Nihilism (Populism), Nihilists is synonymous with assassination and Populist Bakunin is also seen as a person who advocates assassination. Second. Anarchism is not the same idea as Nihilism. Bakunin was both a populist and an anarchist, and because of his dual identity, early Japanese intellectuals mistakenly believed that Anarchism is Nihilists. So the assassination referred to by Nihilists was transferred to Anarchism again. Since then, Anarchism has been closely integrated with assassination.