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      • KCI등재
      • KCI등재

        Development of Enzymatic Recombinase Amplification Assays for the Rapid Visual Detection of HPV16/18

        Ding Ning,Qi Wanwan,Wu Zihan,Zhang Yaqin,Xu Ruowei,Lin Qiannan,Zhu Jin,Zhang Huilin 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.8

        Human papillomavirus (HPV) types 16 and 18 are the major causes of cervical lesions and are associated with 71% of cervical cancer cases globally. However, public health infrastructures to support cervical cancer screening may be unavailable to women in low-resource areas. Therefore, sensitive, convenient, and cost-efficient diagnostic methods are required for the detection of HPV16/18. Here, we designed two novel methods, real-time ERA and ERA-LFD, based on enzymatic recombinase amplification (ERA) for quick point-of-care identification of the HPV E6/E7 genes. The entire detection process could be completed within 25 min at a constant low temperature (35–43°C), and the results of the combined methods could be present as the amplification curves or the bands presented on dipsticks and directly interpreted with the naked eye. The ERA assays evaluated using standard plasmids carrying the E6/E7 genes and clinical samples exhibited excellent specificity, as no cross-reaction with other common HPV types was observed. The detection limits of our ERA assays were 100 and 101 copies/μl for HPV16 and 18 respectively, which were comparable to those of the real-time PCR assay. Assessment of the clinical performance of the ERA assays using 114 cervical tissue samples demonstrated that they are highly consistent with real-time PCR, the gold standard for HPV detection. This study demonstrated that ERA-based assays possess excellent sensitivity, specificity, and repeatability for HPV16 and HPV18 detection with great potential to become robust diagnostic tools in local hospitals and field studies.

      • KCI등재

        무설탕 차음료의 포장 디자인 요소와 지각된 가치가 소비자의 구매행동에 미치는 영향에 관한 연구

        NING DING,진무위,윤경준 한국국제상학회 2024 국제상학 Vol.39 No.2

        본 연구는 중국 음료 시장에서 열풍을 일으키고 있는 무설탕 차음료를 대상으로 포장 다자인 요소와 지각된 가치가 소비자의 구매행동에 미치는 영향을 분석하고자 한다. 이를 위해 본 연구는 2024년 5월 5일부터 14일까지 중국 본토 소비자를 대상으로 온라인 설문조사를 실시하여 412개의 유효표본을 수집하였으며, 구조방정식 모형을 활용하였다. 분석결과, 무설탕 차음료의 포장 디자인 요소와 지각된 가치 중 정보 명확성과 문화적 가치가 큰 영향(+)을 미치는 반면에 시각적 미학과 건강 가치는 비교적으로 영향력이 낮은 것으로 분석되었다. 또한, 포장 디자인 요소와 지각된 가치가 구매행동에 영향을 미치는 관계에서 만족도는 매개 효과가 있는 것으로 나타났다. 본 연구의 결과를 통해 무설탕 차음료 포장 디자인의 시각적 요소보다 정보 전달의 정확성과 효율성, 문화적 요소, 건강 개념, 정서적 요소를 통합한 친환경 포장은 소비자에게 더욱 중요한 것으로 확인되었다. 본 연구는 향후 중국기업뿐만 아니라 중국 시장에 진출하고자 하는 한국기업도 참고자료로 활용될 것으로 기대된다. The purpose of this study is to help brands more accurately understand market trends and consumer expectations, thereby guiding targeted adjustments and innovations in packaging design for Sugar-Free Tea. This paper conducted an online survey of mainland Chinese consumers from May 5 to 14, 2024, collecting 412 valid samples. SPSS 26.0 and AMOS 23.0 were used for data analysis and structural equation modeling. According to the study, among the packaging design elements and perceived value dimensions of sugar-free tea, information clarity and cultural value are most influential, while visual aesthetics and health value have least impact. Additionally, satisfaction mediates the relationship between packaging design, perceived value, and purchasing behavior. Compared to elegant and sophisticated designs, eco-friendly packaging that is clear in information and incorporates cultural elements, health concepts, and emotional factors significantly enhances consumer satisfaction, promoting purchasing behavior.

      • KCI등재

        Photography as Key Reference and Artwork

        Ding Ning(딩닝) 현대미술사학회 2016 현대미술사연구 Vol.0 No.39

        본 논문은 1950년대부터 21세기 초반에 이르기까지의 중국 현대 회화의 역사에서 참조자료의 역할을 해 온 사진에 대해 다룬다. 그리고 이러한 관점에서 가장 영향력 있는 중국 역사화인 동 쉔(Dong Xiwen)의 〈중화인민공화국 건국 기념식(The Founding Ceremony of the People’s Republic)〉과 첸 지엔(Chen Jian)의 〈1945년 9월 9일 난징(Nanjing at 9, 9 September 1945 AD)〉 두 작품을 분석하고자 한다. 중국 현대미술사에서 사진은 회화의 참조자료로서의 역할을 충실히 수행했을 뿐 아니라 1970년대 후반 이후에는 독자적인 예술 작품으로서 전시되고 수집되기에 이르렀다. 그리고 몇몇 훌륭한 사진 전시들이 대중적으로 큰 인기를 끌고 국제 미술계에서 호평을 받기도 하였다. The present paper deals with the role of photography as the key reference for contemporary oil painting in China from the 1950s through the early 21st century. Two most influential and representative oil paintings of historical subjects are discussed in detail: Dong Xiwen’s The Founding Ceremony of the People’s Republic and Chen Jian’s Nanjing at 9, 9 September 1945 AD. After the late 1970s, photography has been displayed and collected as artworks in Chinese museums and galleries. This paper explores the ways in which Some special photography exhibitions not only attracted big audience domestically but were also welcomed internationally.

      • KCI등재

        Physalin D inhibits RANKL-induced osteoclastogenesis and bone loss via regulating calcium signaling

        ( Ning Ding ),( Yanzhu Lu ),( Hanmin Cui ),( Qinyu Ma ),( Dongxia Qiu ),( Xueting Wei ),( Ce Dou ),( Ning Cao ) 생화학분자생물학회(구 한국생화학분자생물학회) 2020 BMB Reports Vol.53 No.3

        We investigated the effects of physalin A, B, D, and F on osteoclastogenesis induced by receptor activator of nuclear factor кB ligand (RANKL). The biological functions of different physalins were first predicted using an in silico bioinformatic tool (BATMAN-TCM). Afterwards, we tested cell viability and cell apoptosis rate to analyze the cytotoxicity of different physalins. We analyzed the inhibitory effects of physalins on RANKL-induced osteoclastogenesis from mouse bone-marrow macrophages (BMMs) using a tartrate-resistant acid phosphatase (TRAP) stain. We found that physalin D has the best selectivity index (SI) among all analyzed physalins. We then confirmed the inhibitory effects of physalin D on osteoclast maturation and function by immunostaining of F-actin and a pit-formation assay. On the molecular level, physalin D attenuated RANKLevoked intracellular calcium ([Ca(2+)](i)) oscillation by inhibiting phosphorylation of phospholipase Cγ2 (PLCγ2) and thus blocked the downstream activation of Ca2+/calmodulindependent protein kinases (CaMK)IV and cAMP-responsive element-binding protein (CREB). An animal study showed that physalin D treatment rescues bone microarchitecture, prevents bone loss, and restores bone strength in a model of rapid bone loss induced by soluble RANKL. Taken together, these results suggest that physalin D inhibits RANKL-induced osteoclastogenesis and bone loss via suppressing the PLCγ2-CaMK-CREB pathway. [BMB Reports 2020; 53(3): 154-159]

      • KCI등재

        Identifying genes for resistant starch, slowly digestible starch, and rapidly digestible starch in rice using genome-wide association studies

        Ning Zhang,Maike Wang,Ji Fu,Yi Shen,Yi Ding,Dianxing Wu,Xiaoli Shu,Wenjian Song 한국유전학회 2020 Genes & Genomics Vol.42 No.11

        Background The digestibility of starch is important for the nutritive value of staple food. Although several genes are responsible for resistant starch (RS) and slowly digestible starch (SDS), gaps persist concerning the molecular basis of RS and SDS formation due to the complex genetic mechanisms of starch digestibility. Objectives The objective of this study was to identify new genes for starch digestibility in rice and interprete the genetic mechanisms of RS and SDS by GWAS. Methods Genome-wide association studies were conducted by associating the RS and SDS phenotypes of 104 re-sequenced rice lines to an SNP dataset of 2,288,867 sites using a compressed mixed linear model. Candidate genes were identifed according to the position of the SNPs based on data from the MSU Rice Genome Annotation Project. Results Seven quantitative trait loci (QTLs) were detected to be associated with the RS content, among which the SNP 6 m1765761 was located on Waxy. Starch branching enzymes IIa (BEIIa) close to QTL qRS-I4 was detected and further identifed as a specifc candidate gene for RS in INDICA. Two QTLs were associated with SDS, and the LOC_Os09g09360 encoding lipase was identifed as a causal gene for SDS. Conclusions GWAS is a valid strategy to genetically dissect the formation of starch digestion properties in rice. RS formation in grains is dependent on the rice type; lipid might also contribute to starch digestibility and should be an alternative factor to improve rice starch digestibility.

      • Computational Fluid Dynamics을 이용한 폐쇄유역의 유동해석

        김태영,( Ding Ning ),조성용,민병준,김민영,이민정,박초아,김홍곤 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.0

        물 산업은 급속한 인구 증가와 환경오염에 따른 물 부족심화 등의 이유로 꾸준히 성장하여 왔으며 미래 유망사업으로 부상하고 있다. 그 중 수처리와 관련된 분야는 물 산업가운데서 큰 비중을 차지하며 따라서 수처리 산업에 사용되는 장비에 대한수요도 점차 증가하는 추세이다. 본 연구에서는 웨이브를 이용한 수처리 장치에서 브레이드의 형태에 따른 유동 특성 분석을 통해 다양한 형태의 브레이드 성능 특성파악을 진행하였고, 이를 토대로 브레이드 성능을 결정하는 물리적 원인 등을 연구하였다.

      • KCI등재

        Estimating Modal Shift by Introducing Transit Priority Strategies under Congested Traffic using the Multinomial Logit Model

        Ling Ding,Ning Zhang 대한토목학회 2017 KSCE JOURNAL OF CIVIL ENGINEERING Vol.21 No.6

        Researchers have been seeking approaches to persuade car users to shift to mass transit through implementing transit priority strategies. Widely used transit priority strategies include a Managed Bus Lane (MBL), transit fare discount, and an increase in parking fees. Most previous studies have focused on the implementation of a single priority strategy, which may not be sufficient enough to induce a significant shift in travel mode. This study aims to investigate the effect of multiple transit priority strategies, also known as multi-strategy, on the travel modal shift of car users. For this purpose, the effects of the three single strategies and the multistrategy on travel modal shift were compared. A logit model was employed to estimate the choice in travel mode based on the Revealed Preference/stated Preference (RP/SP) data. The findings in this study were compared with those reported in previous studies as well. The results showed that the increase in parking fees has the most influential impact on the travel modal shift of car users, followed by the MBL. Comparatively, a transit fare discount was found to be unattractive for car users to make a change to mass transit. On the other hand, the multi-strategy showed remarkably higher effects on the travel modal shift of car users, but is not a simple superposition of the three single strategies. Additionally, the estimated probability of the shift in transit sharing rate based on RP/SP data was found to be much higher than the actual sharing rate, indicating that the SP data may overestimate the respondents’ actual choice.

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