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Ultrasonic Vibration Assisted Cutting of Nomex Honeycomb Core Materials
Dao‑Hui Xiang,Bang‑Fu Wu,Yun‑Long Yao,Bo Zhao,Jin‑Yuan Tang 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.20 No.1
Nomex honeycomb core materials have been widely used in the aviation industry due to their special structure and performance. Conventional high-speed machining have resulted in the poor machinability of the honeycomb core so that the ultrasonic machining technology was applied. The kinematic characteristics in the ultrasonic vibration assisted cutting process were analyzed according to the movement of the sharp tool. Based on slide effect, a cutting force model was proposed to study the relationship between cutting parameters and cutting force. Ultrasonic vibration assisted cutting and ordinary cutting tests of Nomex honeycomb core material were conducted by considering feed rate, the inclined angle and the deflected angle. Besides, the effects of cutting parameters on machined surface quality of honeycomb core wall were studied. The test results show that slide effect caused by ultrasonic vibrations can reduce cutting resistance compared with ordinary cutting. The developed cutting force model can be applied to evaluated the cutting force in the ultrasonic vibration assisted cutting of Nomex honeycomb core material. The inclined angle has a great influence on the cutting force during ultrasonic vibration assisted cutting. High-speed reciprocating sliding action can effectively cut aramid fibers so that burrs and tearing defects of the incision have been greatly improved under condition of ultrasonic vibration assisted cutting.
Human Liver Specific Transcriptional Factor TCP10L Binds to MAD4
( Dao Jun Jiang ),( Hong Xiu Yu ),( Sa Yin Hexige ),( Ze Kun Guo ),( Xiang Wang ),( Li Jie Ma ),( Zheng Chen ),( Shou Yuan Zhao ),( Long Yu ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.4
A human gene T-complex protein 10 like (TCP10L) was cloned in our lab. A previous study showed that it expressed specifically in the liver and testis. A transcription experiment revealed that TCP10L was a transcription factor with transcription inhibition activity. In this study, the human MAD4 was identified to interact with TCP10L by a yeast two-hybrid screen. This finding was confirmed by immunoprecipitation and subcellular localization experiments. As MAD4 is a member of the MAD family, which antagonizes the functions of MYC and promotes cell differentiation, the biological function of the interaction between TCP10L and MAD4 may be to maintain the differentiation state in liver cells. Also, we propose that the up-regulation of Myc is caused by the down-regulation of TCPIOL in human hepatocarcinomas.
Human Liver Specific Transcriptional Factor TCP10L Binds to MAD4
Jiang, Dao-Jun,Yu, Hong-Xiu,Hexige, Sa-Yin,Guo, Ze-Kun,Wang, Xiang,Ma, Li-Jie,Chen, Zheng,Zhao, Shou-Yuan,Yu, Long Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.4
A human gene T-complex protein 10 like (TCP10L) was cloned in our lab. A previous study showed that it expressed specifically in the liver and testis. A transcription experiment revealed that TCP10L was a transcription factor with transcription inhibition activity. In this study, the human MAD4 was identified to interact with TCP10L by a yeast two-hybrid screen. This finding was confirmed by immunoprecipitation and subcellular localization experiments. As MAD4 is a member of the MAD family, which antagonizes the functions of MYC and promotes cell differentiation, the biological function of the interaction between TCP10L and MAD4 may be to maintain the differentiation state in liver cells. Also, we propose that the up-regulation of Myc is caused by the down-regulation of TCP10L in human hepatocarcinomas.
Qian, Li-Yuan,Li, Ping,Li, Xiao-Rong,Chen, Dao-Jin,Zhu, Shai-Hong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8
Aims: To explore the relationship between various molecular makers and liver metastasis of colorectal cancer (CRC). Method: Using immunohistochemistry, protein expression of CEA, nm23, c-met, MMP2, COX-2, VEGF, EGFR, and CD44 was assessed in 80 CRC cases. The Chi-square test and logistic regression were performed to analyze the relationship between these indicators and CRC liver metastasis. Results: There were significant differences in expression of CEA, MMP2, CD44, VEGF and EGFR between the liver metastasis and non metastasis groups (P < 0.05); no significant differences were noted for nm23, c-met, and COX-2 expression. Logistic regression analysis showed that only CEA, VEGF, and EGFR entered into the regression equation, and had significant correlations with CRC liver metastasis (${\alpha}$ inclusion= 0.10, ${\alpha}$ elimination = 0.15, R2 = 0.718). Conclusions: Combination detection of CEA, VEGF, and EGFR may be an effective means to predict CRC liver metastasis. Nm23, c-met, MMP2, COX-2, and CD44, in contrast, are not suitable as prognostic markers.
Yan-Ping Li,Dao-Bang Tang,Xiao-Qiang Wang,Meng Wang,Qing-Feng Zhang,Yuan Liu,Bei-Yun Shen,Jiguang Chen,Zhongping Yin 한국생물공학회 2021 Biotechnology and Bioprocess Engineering Vol.26 No.3
Three types of calli were induced from Origanum vulgare (O. vulgare) aseptic seedlings, and the friable calli with white appearance and high growth rate were further screened and used to develop cell suspension culture to produce polyphenols. Murashige and Skoog (MS) medium with 3.0 mg/L Kinetin (KT) and 0.5 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) was suitable for both O. vulgare cells growth and polyphenols accumulation. To further enhance the polyphenols accumulation, O. vulgare cells were treated by phenylalanine (Phe) feeding and salicylic acid (SA) elicitation. Compared with the individual Phe feeding and SA elicitation, SA elicitation combined with Phe feeding showed a much better promotion effect on the polyphenols synthesis in O. vulgare cells, especially rosmarinic acid (RosA) accumulation. With the combined treatment of 200 μM SA and 100 μM Phe, total polyphenols content and yield were 41.36 mg/g and 752.93 mg/L, respectively. RosA content and yield reached 31.25 mg/g and 570.37 mg/L, which were 5.44 and 5.47 times that of the control. Furthermore, the total polyphenols extracted from the cultured cells treated by SA elicitation combined with Phe feeding displayed a much higher antioxidant capacity than that of untreated cells, meanwhile its 1,1-diphenyl-2- trinitrophenyl hydrazine (DPPH) and superoxide anion radical-scavenging activity were much stronger than that of vitamin C. What’s more, our results also showed that RosA was the principal contributor to the fine antioxidant capacity of the total polyphenols extracted from the SA and Phe treated cells. Our research indicated that SA elicitation combined with Phe feeding significantly improved the polyphenols yield and antioxidant capacity of the cultured O. vulgare cells, and therefore has a promising application prospect in natural polyphenols production.
Liu, Bei Hui,Dao Hai Zhang,Lee, Yuan Kun 한국미생물 · 생명공학회 2000 Journal of microbiology and biotechnology Vol.10 No.2
The freshwater green alga Chlorococcum sp. grew on NH_4^+, NO_3^-, urea, yeast extract, and peptone as the nitrogen source showing similar patterns of growth and secondary carotenoid (SC) production. However, the most suitable nitrogen source for the induction of SC was urea. The effects of nutrient levels (urea, phosphate, sulfate, ferrous iron, and salt) on growth and SC production were studied by varying the concentration of each nutrient in batch cultures. High biomass production was achieved in cultures containing 20- 28mM urea, 4.8-10mM phosphate, 1.6mM sulfate, 70mM NaCl, and 20-100μM iron. The highest SC content in biomass was obtained when cultured at 5-10mM urea, 12mM phosphate, 1.6mM sulfate, 170mM NaCl, and 50μM iron. The optimum concentrations of nutrients for biomass and for the SC accumulation in biomass were evaluated and the two media for achieving high biomass production and SC production were thus developed. The extent to which each parameter to stimulate the formation of SC in the alga were varied and the potentially improved SC production by manipulating the nutrient levels in the modified media were discussed.
Flexural performance of fire damaged and rehabilitated two span reinforced concrete slabs and beams
Jiang-Tao Yu,Yuan Liu,Zhou-Dao Lu,Kai Xiang 국제구조공학회 2012 Structural Engineering and Mechanics, An Int'l Jou Vol.42 No.6
Five two-span reinforced concrete (RC) slabs and seven two-span RC beams were tested under the ISO 834 standard fire with different durations. CFRP strengthening was then applied to some of the specimens after the damaged concrete was removed from the specimens and replaced with polymer mortar. All the specimens were loaded to failure to investigate the influence of fire-damage and the effectiveness of strengthening methods. Test results indicated that the flexural capacities of specimens decrease with the fire duration increases. Moreover, fire exposure had more significant effect on the flexural rigidity than on the bearing capacity of the specimens. After rehabilitation, the bearing capacities of specimens reached or even exceeded that of the reference RC specimen, and the strengthening methods seemed to have limited effect on flexural rigidity recovery. From the analysis of moment redistribution of tested beams, elevated temperature is found having different impacts on sagging moment region and hogging moment region. The damage of RC continuous member is definitely a comprehensive response of different regions.
Flexural performance of fire damaged and rehabilitated two span reinforced concrete slabs and beams
Yu, Jiang-Tao,Liu, Yuan,Lu, Zhou-Dao,Xiang, Kai Techno-Press 2012 Structural Engineering and Mechanics, An Int'l Jou Vol.42 No.6
Five two-span reinforced concrete (RC) slabs and seven two-span RC beams were tested under the ISO 834 standard fire with different durations. CFRP strengthening was then applied to some of the specimens after the damaged concrete was removed from the specimens and replaced with polymer mortar. All the specimens were loaded to failure to investigate the influence of fire-damage and the effectiveness of strengthening methods. Test results indicated that the flexural capacities of specimens decrease with the fire duration increases. Moreover, fire exposure had more significant effect on the flexural rigidity than on the bearing capacity of the specimens. After rehabilitation, the bearing capacities of specimens reached or even exceeded that of the reference RC specimen, and the strengthening methods seemed to have limited effect on flexural rigidity recovery. From the analysis of moment redistribution of tested beams, elevated temperature is found having different impacts on sagging moment region and hogging moment region. The damage of RC continuous member is definitely a comprehensive response of different regions.