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RISS 인기검색어
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- 저자 : D.H. Wei (검색결과 72 건)
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Escherichia coli-based expression of functional novel DNA-binding histone H1 from Carassius auratus
Wei, Q.,Jung, H.J.,Hwang, D.S.,Hwang, B.H.,Gim, Y.,Cha, H.J. IPC Science and Technology Press ; Elsevier Scienc 2007 Enzyme and microbial technology Vol.40 No.6
Histones are DNA-binding proteins that assist in DNA packaging and protection. Here, we, for the first time, cloned a novel histone H1 cDNA (638bp) from the goldfish, Carassius auratus. Sequencing revealed that this histone H1 shared 68.1% amino acid identity and 73.9% similarity with that from the rainbow trout, Salmo gairdneri. We successfully expressed a full-length recombinant version (∼20kDa) and a truncated C-terminal fragment (∼6kDa; 61 amino acids) of this histone H1 as a partially soluble form using a maltose binding protein (MBP) fusion strategy in an Escherichia coli expression system. Our results revealed that both these recombinant histone H1 versions had DNA binding and protection functions, and MBP fusion system was an effective way to produce biological functional recombinant histone proteins in E. coli. This novel recombinant histone H1 protein and/or its C-terminal peptide could be used as potential mediators for efficacious gene delivery.
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China Spallation Neutron Source: Accelerator Design Iterations and R&D Status
J. Wei,C.-D. Deng,C.-H. Wang,C.-T. Shi,H. Sun,H.-F. Ouyang,H.-M. Qu,H.-Y. Dong,J. Li,J. Zhang,J.-S. Cao,J.-Y. Tang,L. Dong,L.-L. Wang,Q. Qin,Q.-B. Wang,S. Wang,S.-N. Fu,S.-X Fang,T. -G. Xu,W. Kang,Y.- 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.50 No.I
The China Spallation Neutron Source (CSNS) is a high-power, accelerator-based project currently under preparation. The accelerator complex consists of an H$^-$ ion source, an H$^-$ linac, a rapid-cycling proton synchrotron, and the transport lines. During the past year, the design of most accelerator systems went through major iterations, and initial research and developments was started on the prototyping of several key components.
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Zeng, Y.Q.,Wang, G.L.,Wang, C.F.,Wei, S.D.,Wu, Y.,Wang, L.Y.,Wang, H.,Yang, H.L. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.1
This study was performed to detect genetic variation of the heart fatty acid-binding protein (H-FABP) gene by PCRRFLPs approach and its association with intramuscular fat (IMF) content. Data from 223 individuals, including one Chinese native pig breed and four western pig breeds, were analyzed. The results showed that for the H-FABP gene, there was one polymorphic HinfI site in the 5'-upstream region, whereas there were one HaeIII and one HinfI (marked as $HinfI^*$) polymorphic site in the second intron, respectively. The three PCR-RFLPs were present in all breeds tested. The allele frequencies, however, revealed significant differences between them (p<0.05). Furthermore, the allele frequency distribution of HinfI in the Laiwu Black and that of $HinfI^*$ in the Hampshire breed were at disequilibrium, which might be the result of selective breeding. Results also indicated that for HinfI, HaeIII and $HinfI^*$ HFABP RFLP, significant (p<0.05) contrasts of 0.78%, -0.69% and 0.72% were detected in the least square means of IMF content between the homozygous genotype HH and hh, DD and dd, BB and bb classes, respectively. It implied that the HHddBB genotype had the highest IMF content in this experimental population and these H-FABP RFLPs could serve, to some extent, as genetic markers for use in improvement of IMF content.
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Wei, D.,Huang, Y.,Kim, S.I.,Yu, Y.M.,Seo, H.J. North-Holland 2013 Materials letters Vol.99 No.-
A series of Eu<SUP>2+</SUP>-activated green-emitting phosphors, barium zinc silicate Ba<SUB>2</SUB>Zn<SUB>3</SUB>Si<SUB>3</SUB>O<SUB>11</SUB>:Eu<SUP>2+</SUP>, was first synthesized by chemical sol-gel method. The photoluminescence excitation and emission spectra, the concentration quenching of the phosphors, the luminescence color chromaticity and absolute quantum efficiency (QE), were measured. The results indicate that Ba<SUB>2</SUB>Zn<SUB>3</SUB>Si<SUB>3</SUB>O<SUB>11</SUB>:Eu<SUP>2+</SUP> phosphors can be efficiently excited by not only UV- but also blue-LED chips to realize novel green-emitting luminescence corresponding to the electric-dipole allowed 4f-5d transition of the Eu<SUP>2+</SUP> ions. Additionally, the luminescence was suggested to originate from two kinds of Eu<SUP>2+</SUP> centers in terms of photoluminescence properties. The energy transfer between the Eu<SUP>2+</SUP> centers was discussed by analyzing the photoluminescence spectra, concentration-dependent luminescence intensity and lifetimes. This is a potential candidate for the application to white LEDs because the tunable luminescence and small thermal quenching.
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