보문산공원 야간이용 활성화 방안

이시영, 이재만, 양뢰, 천현우 배재대학교 자연과학연구소 2013 自然科學論文集 Vol.24 No.1

This study is aimed to find ways to analyze the current nighttime use of mountainside Mt.Bomunsan Park and thereby, to increase the efficiency of mountainside parks situated in urban areas, by case-studying other similar parks whose nighttime use has been facilitated. Also, the study focused on identifying diverse leisure activities for urban citizens to enjoy at night at such parks. First, the Mt.Boumunsan Park area was analyzed in its current nighttime use, space by space, to learn how the park is used by the public at night. Second, other parks whose physical environment is similar to that of Mt.Boumunsan Park were case-studied so that the result could be reflected on the operation of Mt.Boumunsan Park. Third, revitalization suggestions were made for the visit to Mt.Boumunsan Park during nighttime so that they can be referred to as basic data when planning and designing a park in the future for more efficient spacing. It is anticipated that when planning and designing is under consideration to improve Mt.Boumunsan Park, it is better to devise, after taking into account the local government's financial status and the public opinions, a short-and mid-term plan will better serve citizens than a non-feasible long-term master plan.

중국 흑룡강성 조선족 생활문화의 지속과 변동 : 의례와 축제를 중심으로 Focusing on rites and festivals

강위원,김시덕,안화춘 한국사진학회 2005 AURA Vol.0 No.12

Farmers and patriots became impoverished by natural disasters and a decline of governmental power at the end of Joseon dynasty, when they were forced to migrate to China for the purpose of either their living or national independence movement. With their descendants, and then, they continued to reside in China even after national independence. They are just called the Joseon tribe in China. In fact, they were subjected to a lot of oppression and exploitation in starting to reside in China, a foreign area. Their living culture is based on the originality of Korean tribe, and they adapted themselves to a variety of new political, cultural and social environments, as well as to local climate and life style. While contacting a different kind of tribes, they also acquired their own knowledge of life. Therefore, their living culture is a version of Korean tribe' s culture. With the recent trend of industrialization with so-called "Korean Dream," their living culture is changing radically and even disappearing gradually. Once a minority race' s culture or life style disappears in the Chinese people-centered society, it can never be recovered. Therefore, it is a very urgent matter to photograph and keep a record of their current culture. It is also our duty to guide the change of their living culture in such a manner that their racial characteristics can be retained in China where a Korean boon is now on.

High Survivin and Low Zinc Finger of the Cerebellum 1 Expression Indicates Poor Prognosis in Triple-negative Breast Carcinoma

Chun-Tao Shi,Jun Ma,Qi-Feng Shi,Ye Zhang,Hao-Nan Wang 한국유방암학회 2019 Journal of breast cancer Vol.22 No.2

Purpose: Triple-negative breast carcinoma (TNBC) is accompanied with high risk of metastasis and recurrence. The present study aimed to explore the clinicopathological and prognostic roles of putative tumor-related genes in patients with TNBC. Methods: Thirty pairs of frozen-thawed tumors were used to select reliable indicators via real-time quantitative polymerase chain reaction (RT-qPCR). Then, 150 pathology specimens were used to evaluate the expression of proteins in TNBC through immunohistochemistry. In addition, Kaplan-Meier curves and Cox regression analysis were also performed to analyze the overall survival and disease-free survival. Results: RT-qPCR results indicated that among all the proteins analyzed using fresh-frozen TNBC samples, the expression levels of only Survivin and zinc finger of the cerebellum 1 (ZIC1) were obviously different from those in the corresponding normal tissues. Survivin and ZIC1 expression had opposite effects on the clinicopathological diagnosis and prognostic assessment in TNBC patients. Further, there was a negative correlation between Survivin and ZIC1 expression. In addition, the “Survivin-positive ZIC1-negative group” was associated with histologic grade, lymph node metastasis, and TNM staging (p < 0.001) and this was also an independent factor for evaluating the prognosis of TNBC in patients. Conclusion: In summary, the expression levels of Survivin and ZIC1 in TNBC are different from those in normal tissues and are negatively correlated mutually. The combined detection of Survivin and ZIC1 expression levels could allow better comprehensive diagnosis and prognostic evaluation for TNBC patients.

Surface-Displayed Porcine IFN-λ3 in Lactobacillus plantarum Inhibits Porcine Enteric Coronavirus Infection of Porcine Intestinal Epithelial Cells

Yong-Shi Liu,Qiong Liu,Yanlong Jiang,Wentao Yang,Hai-Bin Huang,Chun-Wei Shi,Gui-Lian Yang,Chun-Feng Wang 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.4

Interferon (IFN)-λ plays an essential role in mucosal cells which exhibit strong antiviral activity. Lactobacillus plantarum (L. plantarum) has substantial application potential in the food and medical industries because of its probiotic properties. Alphacoronaviruses, especially porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV), cause high morbidity and mortality in piglets resulting in economic loss. Co-infection by these two viruses is becoming increasingly frequent. Therefore, it is particularly important to develop a new drug to prevent diarrhea infected with mixed viruses in piglets. In this study, we first constructed an anchored expression vector with CWA (C-terminal cell wall anchor) on L. plantarum. Second, we constructed two recombinant L. plantarum strains that anchored IFN-λ3 via pgsA (N-terminal transmembrane anchor) and CWA. Third, we demonstrated that both recombinant strains possess strong antiviral effects against coronavirus infection in the intestinal porcine epithelial cell line J2 (IPEC-J2). However, recombinant L. plantarum with the CWA anchor exhibited a more powerful antiviral effect than recombinant L. plantarum with pgsA. Consistent with this finding, Lb.plantarum-pSIP-409-IFN- λ3-CWA enhanced the expression levels of IFN-stimulated genes (ISGs) (ISG15, OASL, and Mx1) in IPEC-J2 cells more than did recombinant Lb.plantarum-pSIP-409-pgsA'-IFN-λ3. Our study verifies that recombinant L. plantarum inhibits PEDV and TGEV infection in IPEC-J2 cells, which may offer great potential for use as a novel oral antiviral agent in therapeutic applications for combating porcine epidemic diarrhea and transmissible gastroenteritis. This study is the first to show that recombinant L. plantarum suppresses PEDV and TGEV infection of IPEC-J2 cells.

Improved Regioselective Mononitration of o-Xylene over HBEA-500 Zeolite Catalyst

Chun J. Shi,Yan F. Tai,Hong T. Liu 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.11

α-Glucosidase Inhibitiors from Seed Extract of Paeonia Lactiflora

( Chun Whan Choi ),( Yeon Hee Choi ),( Mi Ran Cha ),( Jee Hee Park ),( Young Sup Kim ),( Young Kyoon Kim ),( Sang Un Choi ),( Gyu Hwan Yon ),( Kyung Sik Hong ),( Young Ho Kim ),( Shi Yong Ryu ) 한국응용생명화학회 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.6

Alpha-glucosidase inhibitors are oral antihyperglycemic agents that act on alpha-glucosidase competitively, delaying intestinal carbohydrate absorption and lessening postprandial increases in glucose levels. In the search for new classes of α-glucosidase inhibitors extracted from natural resources, 420 different plant extracts were evaluated for their inhibitory effect on α-glucosidase, in vitro. Amongst the tested extracts, the seed extract of Paeonia lactiflora (Paeoniaceae) demonstrated a significant degree of inhibition on the enzyme, according to dose. Extensive bioassay-guided fractionation of the extract resulted in the isolation of six materials, luteolin (1), resveratrol (2), trans-ε-viniferin (3), gnetin H (4), suffruticosol A (5) and suffruticosol B (6), that are active agents for α-glucosidase inhibition. Resveratrol (2) is a well-known naturally occurring hydroxystilbene, particularly abundant in wine and some related stilbene oligomers. Materials (3-6) exhibited relatively strong inhibition of α-glucosidase and their IC50 values were calculated as: 0.92mM (2), 3.15mM (3), 1.15 mM (4), 2.53mM (5) and 3.15mM (6). In contrast, the (IC50) value of the reference drug, acarbose, was estimated as 8.28mM in vitro.

Association of FOXJ1 polymorphisms with systemic lupuserythematosus and rheumatoid arthritis in Korean population

Chun-Shi Li,Qinggao Zhang,임미경,신동혁,심승철,Ji-Young Kim,이신석,Ki-Jung Yun,Hyung-Bae Moon,정헌택,채수천 생화학분자생물학회 2007 Experimental and molecular medicine Vol.39 No.6

The forkhead-box J1 (FOXJ1) transcription factor could suppress a spontaneous activation of T cells and κBβ that results in re-pression of NF-κB activity. In Foxj1 deficiency mice, systemic autoimune inflamation is quite comon symptom. Therefore, deregulated Foxj1 is suposed to be associated with autoimmune diseases and/or other inflammatory diseases. Previously, we identified that polymorphisms of human FOXJ1 gene (g.460C>T, g.1805G>T and g.3375G>C) are associated with al-lergic rhinitis in a Korean population. In present study, we compared the genotype and allele frequencies of these SNPs between healthy controls and systemic lu-pus erythematosus (SLE) or rheumatoid arthritis (RA) patients. We also investigated the relationships be-twen each genotype and the expression levels of anti- nuclear antibodies in SLE patients, and rheumatoid patients. The frequencies of haplotypes constructed by these FOXJ1 SNPs were compared between con-trols and SLE (or RA) patients. The results of genotype and allele analysis showed that the prevalence of poly-morphism g.3375G>C was associated with the sus-ceptibility of SLE (P = 0.0072 and 0.0042, respectively). But no significant association was found with RA. In the haplotype analysis, however, the main CGG patients (P = 0.048).

DBOP specifies palea development by suppressing the expansion of the margin of palea in rice

Chun-Hai Shi,Dong-Dong Zeng,Ran Qin,Md. Alamin,Rong Liang,Cheng-Cong Yang,Xiao-Li Jin 한국유전학회 2016 Genes & Genomics Vol.38 No.11

Palea and lemma are grass specific floral organs, and their identities to the typical floral organs in eudicots remains unclear. In the present study, we identified and characterized a defective body of palea (dbop) from ethyl methyl sulfonate mutagenesis lines of Oryza sativa ssp. japonica cv. Nipponbare. The dbop mutant exhibited abnormal palea without the body of palea. By map-based cloning, the DBOP was narrowed down to a 68.9 kb interval on the long arm of chromosome 9. Sequencing result showed that there was G to A substitution and C to A substitution at the 245th and 383rd nucleotide of LOC_Os09g24480 coding sequence region, respectively. DBOP was found allelic to the RETARDED PALEA1 (REP1) gene which encoded a TCP family transcription factor. DBOP/REP1 might specify palea development by suppressing the expansion of the margin of palea.

Correction of Figure. Analysis and Experimental Characterization of Low Speed Direct Drive Fractional Slot Concentrated Winding Surface Permanent Magnet Synchronous Motor with Consequent Pole Rotor

Shi-Uk Chung,Yon-Do Chun,Seok-Hwan Moon 대한전기학회 2016 Journal of Electrical Engineering & Technology Vol.11 No.5

Inhibition of Primary Roots and Stimulation of Lateral Root Development in Arabidopsis thaliana by the Rhizobacterium Serratia marcescens 90-166 Is through Both Auxin-Dependent and -Independent Signaling Pathways

Shi, Chun-Lin,Park, Hyo-Bee,Lee, Jong-Suk,Ryu, Sang-Ryeol,Ryu, Choong-Min Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.29 No.3

The rhizobacterium Serratia marcescens strain 90-166 was previously reported to promote plant growth and induce resistance in Arabidopsis thaliana. In this study, the influence of strain 90-166 on root development was studied in vitro. We observed inhibition of primary root elongation, enhanced lateral root emergence, and early emergence of second order lateral roots after inoculation with strain 90-166 at a certain distance from the root. Using the DR5::GUS transgenic A. thaliana plant and an auxin transport inhibitor, N-1-naphthylphthalamic acid, the altered root development was still elicited by strain 90-166, indicating that this was not a result of changes in plant auxin levels. Intriguingly, indole-3-acetic acid, a major auxin chemical, was only identified just above the detection limit in liquid culture of strain 90-166 using liquid chromatography-mass spectrometry. Focusing on bacterial determinants of the root alterations, we found that primary root elongation was inhibited in seedlings treated with cell supernatant (secreted compounds), while lateral root formation was induced in seedlings treated with lysate supernatant (intracellular compounds). Further study revealed that the alteration of root development elicited by strain 90-166 involved the jasmonate, ethylene, and salicylic acid signaling pathways. Collectively, our results suggest that strain 90-166 can contribute to plant root development via multiple signaling pathways.