DBOP specifies palea development by suppressing the expansion of the margin of palea in rice

Chun-Hai Shi,Dong-Dong Zeng,Ran Qin,Md. Alamin,Rong Liang,Cheng-Cong Yang,Xiao-Li Jin 한국유전학회 2016 Genes & Genomics Vol.38 No.11

Palea and lemma are grass specific floral organs, and their identities to the typical floral organs in eudicots remains unclear. In the present study, we identified and characterized a defective body of palea (dbop) from ethyl methyl sulfonate mutagenesis lines of Oryza sativa ssp. japonica cv. Nipponbare. The dbop mutant exhibited abnormal palea without the body of palea. By map-based cloning, the DBOP was narrowed down to a 68.9 kb interval on the long arm of chromosome 9. Sequencing result showed that there was G to A substitution and C to A substitution at the 245th and 383rd nucleotide of LOC_Os09g24480 coding sequence region, respectively. DBOP was found allelic to the RETARDED PALEA1 (REP1) gene which encoded a TCP family transcription factor. DBOP/REP1 might specify palea development by suppressing the expansion of the margin of palea.

Surface-Displayed Porcine IFN-λ3 in Lactobacillus plantarum Inhibits Porcine Enteric Coronavirus Infection of Porcine Intestinal Epithelial Cells

Yong-Shi Liu,Qiong Liu,Yanlong Jiang,Wentao Yang,Hai-Bin Huang,Chun-Wei Shi,Gui-Lian Yang,Chun-Feng Wang 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.4

Interferon (IFN)-λ plays an essential role in mucosal cells which exhibit strong antiviral activity. Lactobacillus plantarum (L. plantarum) has substantial application potential in the food and medical industries because of its probiotic properties. Alphacoronaviruses, especially porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV), cause high morbidity and mortality in piglets resulting in economic loss. Co-infection by these two viruses is becoming increasingly frequent. Therefore, it is particularly important to develop a new drug to prevent diarrhea infected with mixed viruses in piglets. In this study, we first constructed an anchored expression vector with CWA (C-terminal cell wall anchor) on L. plantarum. Second, we constructed two recombinant L. plantarum strains that anchored IFN-λ3 via pgsA (N-terminal transmembrane anchor) and CWA. Third, we demonstrated that both recombinant strains possess strong antiviral effects against coronavirus infection in the intestinal porcine epithelial cell line J2 (IPEC-J2). However, recombinant L. plantarum with the CWA anchor exhibited a more powerful antiviral effect than recombinant L. plantarum with pgsA. Consistent with this finding, Lb.plantarum-pSIP-409-IFN- λ3-CWA enhanced the expression levels of IFN-stimulated genes (ISGs) (ISG15, OASL, and Mx1) in IPEC-J2 cells more than did recombinant Lb.plantarum-pSIP-409-pgsA'-IFN-λ3. Our study verifies that recombinant L. plantarum inhibits PEDV and TGEV infection in IPEC-J2 cells, which may offer great potential for use as a novel oral antiviral agent in therapeutic applications for combating porcine epidemic diarrhea and transmissible gastroenteritis. This study is the first to show that recombinant L. plantarum suppresses PEDV and TGEV infection of IPEC-J2 cells.

구조화 조명 영상에 Dynamic Programming 을 사용한 신뢰도 높은 거리 측정 방법

왕실 ( Shi Wang ),김형석 ( Hyong-suk Kim ),런천신 ( Chun-shin Lin ),진홍신 ( Hong-xin Chen ),임해평 ( Hai-ping Lin ) 한국인터넷정보학회 2008 인터넷정보학회논문지 Vol.9 No.3

구조화 조명을 사용한 거리측정에 동적계획법을 적용함으로써 거리 측정의 정확성이 대폭 향상된 방법을 제안하였다. 구조화 조명을 사용한 거리 측정방법은 거리정보가 조명에 해당하는 화소의 위치에 의해 계산될 수 있다는 점을 이용한 것이다. 그러나, 이 구조화 조명 빛이 물체의 표면에서 흡수되거나 반사됨으로서 흐리거나 잘 보이지 않는 경우가 많다. 이 문제를 해결하기 위해서 본 연구에서는 동적계획법을 사용하였다. 동적 계획법을 위한 셀간 비용(cost)값은 화소 밝기 값의 역수를 사용하였으며, 상단과 하단에 각각 시작선 혹은 목표선을 설정하였다. 이 알고리즘의 장점은 동적 계획 법의 최적화 능력을 사용하므로, 구조화 조명선의 약한 흔적이나 부분적으로 절단된 조명선 위치도 잘 찾아낼 수 있다. 이 알고리즘을 사용하여 다양한 3 차원 물체를 복원한 실험 결과를 제시하였다. An algorithm for tracking the trace of structured light is proposed to obtain depth information accurately. The technique is based on the fact that the pixel location of light in an image has a unique association with the object depth. However, sometimes the projected light is dim or invisible due to the absorption and reflection on the surface of the object. A dynamic programming approach is proposed to solve such a problem. In this paper, necessary mathematics for implementing the algorithm is presented and the projected laser light is tracked utilizing a dynamic programming technique. Advantage is that the trace remains integrity while many parts of the laser beam are dim or invisible. Experimental results as well as the 3-D restoration are reported.

Effects of Oxygen Content on the Simultaneous Microbial Removal of SO2 and NOx in Biotrickling Towers

Xing Chun Wang,Xiao Yi Bi,Pei Shi Sun,Jin Quan Chen,Ping Zou,Xiao Ming Ma,Jing Zhang,Hai Yu Wang,Xiao Yi Xu 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.5

This study uses microbial methods to research the influence of oxygen (O2) content on the removal efficiency of sulfur dioxide (SO2) and nitrogen oxides (NOx) in a tandem twin-towers desulfurization and denitrification process system. Oxygen can play a significant role in biotrickling towers. Other important factors had already been optimized prior to the study, including inlet concentration, gas flow rate, and temperature. SO2 and NOx were prepared by a chemical method. A gas flow meter was used to regulate nitrogen (N2) that had been stored in steel cylinders. In this way, the O2 content was adjusted in the biotrickling towers by controlling the N2 flow rate. Five gradients of O2 content were selected for investigation, namely 4, 8, 12, 16, and 20%. Results indicated that the SO2 removal efficiency from mixed gas (SO2 and NOx) can reach 100%, from all of the five O2 gradients, in biotrickling towers. In a tandem twin-towers desulfurization and denitrification process system, the NOx removal efficiency and the inlet concentration of nitrogen dioxide (NO2) gradually increased as the O2 content increased. Specifically, the average removal efficiency of NOx increased from 49.28 to 80.85% as the O2 content changed from 4 to 20%. The oxygen levels influenced the removal of NOx but the SO2 removal efficiency in mixed gas was always stable.

Preparation of AgCl/Ag₃PO₄/Diatomite Composite by Microemulsion Method for Rapid Photo-Degradation of Rhodamine B with Stability under Visible Light

Zhu, Hai-Tao,Ren, Qi-Fang,Jin, Zhen,Ding, Yi,Liu, Xin-Yu,Ni, Xi-Hui,Han, Meng-Li,Ma, Shi-Yu,Ye, Qing,Oh, Won-Chun Materials Research Society of Korea 2020 한국재료학회지 Vol.30 No.8

In this paper, AgCl/Ag₃PO₄/diatomite photocatalyst is successfully synthesized by microemulsion method and anion in situ substitution method. X-ray diffraction (XRD), photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), transmission electron microscopy (TEM), and ultraviolet-visible spectroscopy (UV-Vis) are used to study the structural and physicochemical characteristics of the AgCl/Ag₃PO₄/diatomite composite. Using rhodamine B (RhB) as a simulated pollutant, the photocatalytic activity and stability of the AgCl/Ag₃PO₄/diatomite composite under visible light are evaluated. In the AgCl/Ag₃PO₄/diatomite visible light system, RhB is nearly 100 % degraded within 15 minutes. And, after five cycles of operation, the photocatalytic activity of AgCl/Ag₃PO₄/diatomite remains at 95 % of the original level, much higher than that of pure Ag₃PO₄ (40 %). In addition, the mechanism of enhanced catalytic performance is discussed. The high photocatalytic performance of AgCl/Ag₃PO₄/diatomite composites can be attributed to the synergistic effect of Ag₃PO₄, diatomite and AgCl nanoparticles. Free radical trapping experiments are used to show that holes and oxygen are the main active species. This material can quickly react with dye molecules adsorbed on the surface of diatomite to degrade RhB dye to CO₂ and H₂O. Even more remarkably, AgCl/Ag₃PO₄/diatomite can maintain above 95 % photo-degradation activity after five cycles.

Balloon dilation of epidermolysis bullosa-related esophageal strictures: A report of two cases

Wei-Zhong Zhou,Jing-Guo Xia,Zheng-Qiang Yang,Chun-Gao Zhou,Lin-Bo Zhao,Sheng Liu,Hai Bin Shi 소화기인터벤션의학회 2018 International journal of gastrointestinal interven Vol.7 No.3

Epidermolysis bullosa (EB) is a very rare inherited disease featured with skin blistering resulting from minor trauma. Sometimes the esophageal mucosa could also be involved, which leads to esophageal strictures. Here we report two cases of EB-related esophageal strictures who were successfully treated with esophageal balloon dilations. The two cases with EB had severe dysphagia. Clinical examination showed signs of malnutrition, skin blisters and loss of toenails due to EB. They underwent careful fluoroscopic balloon dilation with 10- and 16-mm-sized balloon catheters, respectively. They could ingest soft and some solid foods after the procedure and maintained during the 20 months and 16 months follow-up periods.

Balloon dilation of epidermolysis bullosa-related esophageal strictures: A report of two cases

Wei-Zhong Zhou,Jing-Guo Xia,Zheng-Qiang Yang,Chun-Gao Zhou,Lin-Bo Zhao,Sheng Liu,Hai Bin Shi 소화기인터벤션의학회 2018 Gastrointestinal Intervention Vol.7 No.3

Epidermolysis bullosa (EB) is a very rare inherited disease featured with skin blistering resulting from minor trauma. Sometimes the esophageal mucosa could also be involved, which leads to esophageal strictures. Here we report two cases of EB-related esophageal strictures who were successfully treated with esophageal balloon dilations. The two cases with EB had severe dysphagia. Clinical examination showed signs of malnutrition, skin blisters and loss of toenails due to EB. They underwent careful fluoroscopic balloon dilation with 10- and 16-mm-sized balloon catheters, respectively. They could ingest soft and some solid foods after the procedure and maintained during the 20 months and 16 months follow-up periods.

Follow-Up of 58 Traumatic Carotid-Cavernous Fistulas after Endovascular Detachable-Balloon Embolization at a Single Center

Xiao-Quan Xu,Sheng Liu,Qing-Quan Zu,Lin-Bo Zhao,Jin-Guo Xia,Chun-Gao Zhou,Wei-Zhong Zhou,Hai-Bin Shi 대한신경과학회 2013 Journal of Clinical Neurology Vol.9 No.2

Background and Purpose This study evaluated the clinical value of detachable-balloon embolization for traumatic carotid-cavernous fistula (TCCF), focusing on the frequency, risk factors, and retreatment of recurrence. Methods Fifty-eight patients with TCCF underwent transarterial detachable-balloon embolization between October 2004 and March 2011. The clinical follow-up was performed every 3months until up to 3 years postprocedure. Each patient was placed in either the recurrence group or the nonrecurrence group according to whether a recurrence developed after the first procedure. The relevant factors including gender, fistula location, interval between trauma and the interventional procedure, blood flow in the carotid-cavernous fistula, number of balloons, and whether the internal carotid artery (ICA) was sacrificed were evaluated. Results All 58 TCCFs were successfully treated with transarterial balloon embolization, including 7 patients with ICA sacrifice. Recurrent fistulas occurred in seven patients during the follow-up period. Univariate analysis indicated that the interval between trauma and the interventional procedure (p=0.006) might be the main factor related to the recurrence of TCCF. The second treatments involved ICA sacrifice in two patients, fistula embolization with balloons in four patients, and placement of a covered stent in one patient. Conclusions Detachable balloons can still serve as the first-line treatment for TCCFs and recurrent TCCFs despite having a nonnegligible recurrence rate. Shortening the interval between trauma and the interventional procedure may reduce the risk of recurrence.