젤여과법을 이용한 토끼 심근 미오신의 분리 및 부분정제

정성탁,김대중,김호덕,라봉진 중앙대학교 의과대학 의과학연구소 1992 中央醫大誌 Vol.17 No.2

Myosin, the major protein required for the production of force and shortening in muscle, is a hexamer consisting two heavy chains (molecular mass∼200kDa) and pairs of light chains (molecular mass∼16 and 20kDa). Myosin and other contractile apparates from the left ventricular myocardium of rabbit were extracted using pyrophospate buffer system and was purified by using the gel filtration. And partially purified each protein component was confirmed by the electrophoresis in non-dissociation and dissociating conditions respectively. On Disc-PAGE of the crude extract, 4 bands clearly appeared and 3 protein components were separated by chromatography in the 5 fractions. In dissociating electrophoresis, each bands were separated into subunit in the pyrophosphate buffer. The first fraction contained the heavy chain of myosin, tropomyosin and myosin light chain 1 and 2. The second and third fraction were composed of tropomyosin and actin and the proteins that had molecular mass smaller than 15kDa were in the fourth and fifith fractions.

실과 교육과정 평가의 기본 모형과 평가 영역에 관한 연구

정성봉,김종우 韓國實科敎育硏究學會 1999 實科敎育硏究 Vol.5 No.2

The purpose of this study was to suggest evaluation basic model and evaluation area of the Practical Arts curriculum evaluation. The study was carried out through review of literature on curriculum, curriculum development and curriculum evaluation. The curriculum evaluation basic model for Practical Arts were established three demensions; aspects of curriculum, levels of curriculum and evaluation methods of the curriculum. The aspects of curriculum include three major areas of curriculum--development, implementation and outcomes. The levels of curriculum include three layers of curriculum in Korea--national, regional and school curriculum. The third dimension represents evaluation methods by which the values of the curriculum are determined. The two evaluation methods include quantitative approach. The curriculum evaluation areas for Practical Arts were established three categories; curriculum development, implementation and outcomes.

실과 수행 평가의 이론적 기초와 연구 동향

정성봉 韓國實科敎育硏究學會 1999 實科敎育硏究 Vol.5 No.3

The purpose of this study was to review the research trends of performance assessment of practical arts education. The study was carried out through review of literature on evaluation theory, performance assessment theory, and practical arts education. The findings of this study were as follows: 1. Through reviewing literatures, the performance assessment approach such as qualitative approach, alternative assessment, process-oriented assessment were discussed in recent years. 2. For performance assessments to be useful, the following issues need to be kept in forefront: time and space restrict, difficulty of large scale enforcement, evaluator's error possibility, internal consistency insufficient, perception shortage and cost-feasibility given current high monetary and human resource costs for development and use. Based on this conclusions, the further researches for applying of performance assessment in practical arts education should be continually.

자연 치유된 원발성 피부 Alternariosis 2예

정예리,장성남,한승경,조흔정,서순봉,이광훈 대한의진균학회 1999 대한의진균학회지 Vol.4 No.2

The treatment of cutaneous altemariosis consists of surgical excision and systemic antifungal agent. However, only reduction or cessation of corticosteroids and immunosuppressive therapy, if it is possible, can be sufficient to treat the lesion in some cases. We present two cases of spontaneously healed primary cutaneous altemariosis. Case 1 was a 73-year-old female patient with diabetes mellitus, heart failure, chronic renal failure and chronic hepatitis. Case 2 was a 64-year-old male patient with iatrogenic Cushing's syndrome and diabetes mellitus. The correction of the underlying diseases resulted in improvement of the primary cutaneous altemariosis. [Kor J Med Mycol 4(2): 137-142]

엔진회전계의 전달함수 산정을 위한 개루우프(Open loop) 시험

鄭泰相,鄭鳳守,金明奎 진주산업대학교 농업기술연구소 1993 農業技術硏究所報 Vol.6 No.-

농용엔진이 부하변동에도 일정한 회전수를 유지할 수 있는 제어기법을 개발코자, 개루우프 시험에 의해 회전계의 동특성을 이용하여, 통계적으로 산출하여 엔진회전계 방정식을 정리하였으며, 그 결과는 아래와 같다. 1. 엔진회전계의 전달함수와 이산시계열 방정식을 이론과 실험결과를 이용하여 정립하였다. 2. 실제 엔진회전수와 전달함수를 이용한 추정회전수가 일치하였다. This research was carried out to find out a revolution equation of the engine system using regression analysis. The dynamic characteristics of revolution part of an engine were measured by open loop test. Thus, a transfer function and a discrete-time equation relating the engine speed as ouput of the control system to the torque equivalant to throttle opening angle as imput of the control system were derived from the results of the open loop test. 1. The transfer function and the discrete time equation of the revolution system of the engine were derived from the control theory and experimental results. The calculated and measured engine speed were in good agreement.

구름 摩擦에 관한 硏究 : 對數減衰率과 接觸幅에 對하여 About the logarithmic decrement and the contact width

鄭寅聖,홍동표,文奉浩 全北大學校 1986 論文集 Vol.28 No.-

In order to make the mechanism of rolling friction clear, a test apparatus by which the rolling friction between a roller and a plate could be measured accurately was arranged. In this paper, contact behaviors and decrement characteristics of the roller bearing are studied between the pair of roller and plate, which is the model of a linear way bearing. The following results are obtained by using STB 2 samples. 1. The rolling friction force f_p varies with the normal load P and the diameter D of the roller; f_p∝P^(5/4) D-(4/5) at normal loads from 10kgf to 50kgf. 2. The rolling friction force f_p increases with the decrement of surface roughness R_a and the hardness H_v of a test specimen. 3. From curves of the logarithmic decrement λ vs. the dimensionless constant μ, λ gets a maximum value at μ>1 and decreases with the increment of μ. The maximum point of λ approximates the value μ=1. 4. Experimental value of the contact width (a) has a a. ∝(D/H_v)^(oㆍs)ㆍ(P/H_v)^n (n=0.3∼0.4), but the contact width of Hertz theory has ∝(DㆍP)^(1/2). The difference between experimental and theoretical value is due to the difference between static contact width of Hertz theory and dynamic one of experiment.

智異山 면양목장 조성에 관한 연구 : Ⅷ報. 智異山 山野草가 緬羊의 繁殖率에 미치는 영향과 羊毛의 加工利用方法, 販路方案, 貯藏法 및 緬羊團地造成時의 經濟性 分析 Ⅷ. Effect of the native herbages in Mt. Chiri on the breeding percentage of sheep and the processing method, way of marketing and storage of wool and economical analysis in the collective feeding of sheep

鄭鉉丞,姜奉泰,李炳五 진주산업대학교 1972 論文集 Vol.9 No.-

This is the report for 1971 of the third year project among the three years plan on the development of sheep range in Mt. Chiri. To investigate the effect of native grasses in Mt. Chiri on the breeding percentage of sheep this experiment was carried out from March 22, 1971 to March 21, 1972. Numbers of experimental animal used in this experiment were 12. Furthermore, the processing method of wool, marketing, economical analysis in the collective feeding and storage were investigated in cooperation with this experiment. The results obtained here were as follows; 1. For the processing method of wool, there are two ways; one of the processing method of wool is the self-processing method which follows the easy method of wool processing and another of the processing method of wool exchange with the good of wool texture company which follows the charge processing method of wool. 2. For the marketing route of wool, because the wool texture company can buy limitlessly wool, there are two ways to sell wool. The one of the way is the direct sell method of wool to the wool texture company and the other of the way is the sell method of wool via the route of circulation mechanism. 3. In the case of economical analysis for the collective feeding of sheep, if farms could raise 3,000 of sheep in the method of collective feeding it could get net income of 150,000 to 300,000 won for 52 days per farm per year. 4. For the storage of wool, to attach of wool fat must be needed and in the case of short period storage, wool must be placed in the arid place and in the case of long period storage, wool should be prevented from the attack of insect and rat, attachment of spurious article such as dust, decrease of weight and denaturation of wool fat. 5. The breeding percentage of sheep fed only native herbages plant was 60%.

지리산 면양목장 조성에 관한 연구 : Ⅰ보. 면양목장 조성 및 면양사육 단지화 조성을 위한 기초조사 Ⅰ. Fundamental Survey of Resources for the Development of Sheep Range and Sheep Farm Grouping

정현승,강봉태 진주산업대학교 1970 論文集 Vol.4 No.-

This survey reports the availability of the various resources such as geography, meteology, traffic condition, soil property, labor resource, pasture distribution and water resource, for the development of sheep range and sheep-farm group, on the first step to the three-year plan in an area around the Forestry Experiment Station of Chinju Agricultural and Forestry Junior Teachnical College in Mt. Chiri. 1. The geographical conditions of the region found to be fit for the establishment of sheep range were as follows; longitude:127˚44'east,latitude:35˚22'north,elevation:600-900m above marine surface level, slope:5-25˚, total area: 280 ha, and administrative district: from high level flats to Yeo-gok and Dusuk village, Yupyung-ri, Samjang-myun, Sanchungkun, Kyung Sang Nam Do, Korea. Especially, eighty seven farm households and Karangip primary school distriduted around this region were found to be the reasonable resources for the development of sheep farm group in this region. 2. The soil of the area was composed of most of sand-loam with PH 5.3∼5.8, 2-5% of grit and 13.3% of humus (4.6-19.3%) among 30∼50㎝ depth of surface soil. These fertile soil properties were found to be fit for the development of pasture. 3. The meteological records of this region were found as follows: frosty period: from October 16 to April 22, yearly mean air temperature: 9.2℃ , ranging from 26.6℃ in August to (-)12 in January, annual rainfall: 2,346mm, yearly mean wind velocity: 6.2m/sec, direction of the wind: north-west in winter and south-east in summer. According to the above results from only one-year observation, the meteological conditions of this region were similar to those of Dae-Kwan Ryung area and were estimated to be favorable for sheep raising. 4. In this region 35 species of wild grasses were prosperous. The green yield of the grasses averaged 1,584㎏ per 10a and 0.87a was required for grazing a sheep. Therefore, it was estimated that about 3,000 heads of sheep would be well raised in this region. The fact that about 30 percent of Mt. Chiri area is available for sheep pasture suggested that 400,000㎏ of wool, or 250,000,000 won in price(equivalent to 1/10 of the total wool imported in 1967)could be gained from grazing about 100,000 heads of sheep in the whole area of Mt. Chiri, 54,000 ha. 5. Sufficient water resources, conclusive of mooring by the farmers around this region, irrigation water, underground water and especially the pond situated in high level flats were found to be available and the quality of water was satisfactory for watering sheep. 6. Concerning the traffic situation of this region, the construction of 1.5㎞ of road to Dusuk village (with 200m of bridge) and three 2㎞ range path from Dusuk village were required. However, such traffic problems are not serious because the sheep farming requires the road only for disposal of wool produced once a year. 7. From the managment survey for 87 farms around this area, it was found that each farm had 1,019.6 hours of unemployed labour per year, which was sufficient for raising 60 heads of sheep. So it could be estimated. that potential labour enough for raising 5,200 heads of sheep was reserved in this area. Moreover, the Karangip primary school boys are available as a good resource of labour for sheep grazing. The great deal of by-products of sericulture and potato-farming, which were the major industries in this region, was available for sheep raising.

實科 敎育 課程 詳細化 硏究

鄭聖鳳,宋賢順 韓國實科敎育硏究學會 1996 實科敎育硏究 Vol.2 No.1

교육과정 상세화는 교수의 질을 향상 시키고 교수 내용을 다양화 시키며, 교수 목표 실현을 위해 필요하다. 이 연구의 목적은 초등학교에서 실과 교육과정을 효과적으로 활용하는데 있다. 이 연구는 크게 세부분으로 이루어져 있다. 실과 교육 목적의 상세화, 실과 교육과정의 단원설정, 실과 교육고정 상세화의 실제가 그것이며, 또한 5, 6학년 실과 교육과정 상세화를 부록에 제시하였다. The curriculum specification be needed to promote the instructional quality, to diversify the teaching contents and to be realized the instruction objectives on practical art education. The purpose of this study was to specified the practical art curriculum for the practical use in elementary school. This study was contained three parts: the specification of practical art curriculum, the contents and units establishment of practical art curriculum, practical specification of practical art curriculum. Also, the specifications of practical art curriculum on 5th and 6th grades were presented in appenices.

유리 전박골피판을 이용한 음경 재건술

정성광,변진석,백봉수,김법완 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.2

Reconstruction of the penis is indicated in a traumatic or surgical amputation, congenital penile absence, micropenis, male pseudohermaproditism, or transsexualism. Initially, penile reconstruction has been performed using a local flap, but often failed because of the multiple procedures, fistula or stricture of the urethra, and no sensation of the phallus. Ideally, penile reconstruction should be a one-stage procedure, creating a phallus with both tactile and aerogenous sensibility, water-tight neourethra allowing for voiding while standing, enough bulk with stiffness, and aesthetically acceptable appearance. Since August 1989 we have constructed a penis in 4 patients. We selected the radial forearm flap as a donor site because it offers a reliable artery, veins, nerves, and stiffner. It also offers thin, malleable skin with adequate width and length. We have followed these patients from 5 months to 10 months. All operations were successful except for one fistula formation.