The H <small>i</small> environment of counter‐rotating gas hosts: gas accretion from cold gas blobs

Chung, Aeree,Bureau, Martin,van Gorkom, J. H.,Koribalski, Bä,rbel Blackwell Publishing Ltd 2012 Monthly notices of the Royal Astronomical Society Vol.422 No.2

<P><B>ABSTRACT</B></P><P>We probe the H <SMALL>i</SMALL> properties and the gas environments of three early‐type barred galaxies harbouring counter‐rotating ionized gas: NGC 128, NGC 3203 and NGC 7332. Each system has one or more optically identified galaxy at a similar or as yet unknown redshift within a 50‐kpc projected radius. Using H <SMALL>i</SMALL> synthesis imaging data, we investigate the hypothesis that the counter‐rotating gas in these galaxies has been accreted from their neighbours. In NGC 128 and NGC 3203, we find 9.6 × 10<SUP>7</SUP> and 2.3 × 10<SUP>8</SUP> M<SUB>⊙</SUB> of H <SMALL>i</SMALL>, respectively, covering almost the entire stellar bodies of dwarf companions that appear physically connected. Both the H <SMALL>i</SMALL> morphology and kinematics are suggestive of tidal interactions. In NGC 7332, we do not find any directly associated H <SMALL>i</SMALL>. Instead, NGC 7339, a neighbour of a comparable size at about 10 kpc, is found with 8.9 × 10<SUP>8</SUP> M<SUB>⊙</SUB> of H <SMALL>i</SMALL> gas. More recently in a single dish observation, however, another group discovered a large H <SMALL>i</SMALL> structure which seems to be an extension of NGC 7339’s H <SMALL>i</SMALL> disc and also covers NGC 7332. All these observations thus suggest that H <SMALL>i</SMALL> gas is being accreted in these three galaxies from their companions, which is likely responsible for the kinematically decoupled gas component present in their central region. In particular, the dynamical friction time‐scales of the nearest neighbours with H <SMALL>i</SMALL> gas of NGC 128 and NGC 3203 are comparable to their orbital time‐scales around the counter‐rotators, several ∼10<SUP>8</SUP> yr, implying that those neighbours will likely soon merge with the primary galaxies, fuelling them with gas. NGC 7332 also appears to be in the merging process with its neighbour through the common H <SMALL>i</SMALL> envelope. Besides, we find some other potential gas donors around NGC 128 and NGC 7332: two H <SMALL>i</SMALL>‐rich galaxies with <IMG src='/wiley-blackwell_img/equation/MNR_20679_mu1.gif' alt ='inline image'/> and 2.5 × 10<SUP>9</SUP> M<SUB>⊙</SUB> at a distance of ≈67 kpc from NGC 128 and two dwarf systems with <I>M</I><SUB>HI</SUB>= 3.9 × 10<SUP>7</SUP> and 7.4 × 10<SUP>7</SUP> M<SUB>⊙</SUB> at ≲100 kpc from NGC 7332. Among the seven H <SMALL>i</SMALL> features identified in this study, three of them are associated with dwarf galaxies, two of which have only been recently identified in a blind survey, while the third one is still not catalogued at optical wavelengths. Considering the incompleteness of existing studies of the faint dwarf galaxy population both in the optical and in H <SMALL>i</SMALL>, accretion from cold gas blobs, presumably gas‐rich dwarfs, is expected to occur even more frequently than what is inferred from such cases that have been observed to date.</P>

H-Y 에 대한 단일클론 항체의 생산 및 그 이용에 관한 연구 1 . H-Y 에 대한 단일클론항체의 생산

심호섭(H . S . Shim),김재화(J . H . Kim),이병철(B . C . Lee),김종배(J . B . Kim),박홍양(H . Y . Park),정길생(K . S . Chung) 한국축산학회 1988 한국축산학회지 Vol.30 No.7

Testis supernatant, a source of H-Y, obtained from BALB/c mice was used to immunize females of same strain. B lymphocytes of mouse producing antibodies to H-Y were fused with SP2/0-Ag 14 myeloma cells and distributed to 384 wells of 96-well microtiter plates. Eighty hybridoma colonies were formed, resulting in 20.8 percent of fusion efficiency. Three strong positive wells from hybridoma colonies were selected for cloning by ELISA and two of them were also found to be positive by indirect immunofluorescence test. Twelve wells of ELISA-positive were selected after cloning and 2D45D4 clones from them were confirmed to produce monoclonal antibodies to H-Y by indirect immunofluorescence test.

Formation of the internal transport barrier in KSTAR

Chung, J.,Kim, H.S.,Jeon, Y.M.,Kim, J.,Choi, M.J.,Ko, J.,Lee, K.D.,Lee, H.H.,Yi, S.,Kwon, J.M.,Hahn, S.-H.,Ko, W.H.,Lee, J.H.,Yoon, S.W. International Atomic Energy Agency 2018 Nuclear fusion Vol.58 No.1

<P>One of key objectives of tokamak experiments is the exploration of enhanced confinement regimes, and the access of the internal transport barrier (ITB) formation is dealt with an important physics issue in the most of major tokamaks. Also, the advanced tokamak scenario with ITB is expected to lead to a continuous reactor with high fusion power density. From that point of view, the formation of the ITB in KSTAR which is designed for long pulse operation capability is very important although its heating and current drive systems are not fully equipped yet. We have therefore assumed that an early injection of the full NBI power (∼5.5 MW) during the current ramp-up would give a chance to form an internal barrier if the plasma could stay in the L-mode. To avoid the H-mode transition, we have produced inboard limited plasmas with detaching from the both upper and lower divertors. Using this approach, an ITB formation during L-mode has been observed which shows improved core confinement. Ion and electron temperature profiles show the barrier clearly in the temperature, and it was sustained for about 7 s in the dedicated experiment. This is the first stationary ITB observed in a full superconducting tokamak. This operation scenario with the ITB could be an alternative way to achieve a high performance regime in KSTAR, and the length of the ITB discharge could be extended even longer. In this paper, we present the formation of the ITB using measured and simulated characteristic profiles.</P>

Electric Probe Measurements at Edge Region During H‐Mode Discharges in KSTAR

Bak, J.G.,Oh, Y.S.,Kim, H.S.,Hahn, S.H.,Yoon, S.W.,Jeon, Y.M.,Xiao, W.W.,Ko, W.H.,Kim, W.C.,Kwak, J.G.,Woo, H.J.,Chung, K.S.,the KSTAR project team, WILEY‐VCH Verlag 2013 Contributions to plasma physics Vol.53 No.1

<P><B>Abstract</B></P><P>Electrical probe measurements are carried out at the scrape‐off‐layer (SOL) and divertor regions in order to investigate the characteristics of edge plasmas during H‐mode discharges in the Korea Superconducting Tokamak Advanced Research (KSTAR) device. Radial profiles of plasma parameters such as electron temperature T<SUB><I>e</I></SUB>, plasma density n<SUB><I>e</I></SUB>, and parallel flow velocity v<I><SUB>‖</SUB></I> are measured by using a fast reciprocating Langmuir probe assembly (FRLPA) at the SOL region. From the FRLPA measurements, it is found that the decay length of temperature λ<I><SUB>Te</SUB></I> and that of density λ<I><SUB>ne</SUB></I> are 2 <I>∼</I> 4 cm and 1 <I>∼</I> 3 cm, respectively. The magnitude of v<I><SUB>‖</SUB></I> near the last closed flux surface (LCFS) is 4 <I>∼</I> 15 km/s. The radial flux due to edge turbulence at the SOL region is investigated by using spectra analysis on electrostatic fluctuation levels such as ion saturation current Ĩ<I><SUB>is</SUB></I> and floating potential <TEX>$ \tilde{\rm V}_f$</TEX> obtained from the FRLPA measurement. The value of the flux is estimated as <I>∼</I> 10<SUP>20</SUP> particle m<SUP>−2</SUP> s <SUP>−1</SUP> near the LCFS. The poloidal distribution of the ion saturation current density j<I><SUB>is</SUB></I> is measured by fixed edge Langmuir probe array (ELPA) at the divertor region, and it is found that the positions of strike points from the ELPA measurement agree well with those reconstructed from the EFIT result using magnetic diagnostic data. From the spectrum analysis on the ELPA measurements at the divertor region during edge localized modes (ELMs) control in the H‐mode discharges, it is observed that the magnitude of <TEX>$ \tilde{\rm j}_{is}$</TEX>(<I>ω</I>) near strike point decreases when the ELMs are suppressed or mitigated (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)</P>

VP2 capsid domain of the H-1 parvovirus determines susceptibility of human cancer cells to H-1 viral infection

Cho, I-R,Kaowinn, S,Song, J,Kim, S,Koh, S S,Kang, H-Y,Ha, N-C,Lee, K H,Jun, H-S,Chung, Y-H Nature America, Inc. 2015 Cancer gene therapy Vol.22 No.5

Although H-1 parvovirus is used as an antitumor agent, not much is known about the relationship between its specific tropism and oncolytic activity. We hypothesize that VP2, a major capsid protein of H-1 virus, determines H-1-specific tropism. To assess this, we constructed chimeric H-1 viruses expressing Kilham rat virus (KRV) capsid proteins, in their complete or partial forms. Chimeric H-1 viruses (CH1, CH2 and CH3) containing the whole KRV VP2 domain could not induce cytolysis in HeLa, A549 and Panc-1 cells. However, the other chimeric H-1 viruses (CH4 and CH5) expressing a partial KRV VP2 domain induced cytolysis. Additionally, the significant cytopathic effect caused by CH4 and CH5 infection in HeLa cells resulted from preferential viral amplification via DNA replication, RNA transcription and protein synthesis. Modeling of VP2 capsid protein showed that two variable regions (VRs) (VR0 and VR2) of H-1 VP2 protein protrude outward, because of the insertion of extra amino-acid residues, as compared with those of KRV VP2 protein. This might explain the precedence of H-1 VP2 protein over KRV in determining oncolytic activity in human cancer cells. Taking these results together, we propose that the VP2 protein of oncolytic H-1 parvovirus determines its specific tropism in human cancer cells.

南韓의 日本腦炎病毒의 疫學的調査 : 第一編:流行前後期에 있어서 人體, 動物體, 鳥類體內의 免疫體調査 Report 1, Serological survey of Japanese Encephalitis Virus in Korea

金慶浩,白承福,崔憲,金正熙,朴鴻直,白永漢,魚瀅順,鄭浩采,양승조 大韓微生物學會 1961 大韓微生物學會誌 Vol.2 No.1

Inhibition of the multidrug and toxin extrusion (MATE) transporter by pyrimethamine increases the plasma concentration of metformin but does not increase antihyperglycaemic activity in humans

Oh, J.,Chung, H.,Park, S.‐,I.,Yi, S. J.,Jang, K.,Kim, A. H.,Yoon, J.,Cho, J.‐,Y.,Yoon, S. H.,Jang, I.‐,J.,Yu, K.‐,S.,Chung, J.‐,Y. Blackwell Publishing Ltd 2016 DIABETES OBESITY AND METABOLISM Vol.18 No.1

<P>We hypothesized that the pharmacodynamic (PD) characteristics of metformin would change with inhibition of the multidrug and toxin extrusion (MATE) transporter, which mediates renal elimination of metformin. Twenty healthy male subjects received two doses (750/500 mg) of metformin, with and without 50 mg of pyrimethamine (a potent MATE inhibitor), with 1 week of washout in between each dose. The PD characteristics of metformin were assessed using oral glucose tolerance tests (OGTTs) before and after the metformin dose. Metformin concentrations in plasma and urine were determined using liquid chromatography‐electrospray ionization‐tandem mass spectrometry. When metformin was co‐administered with pyrimethamine, its area under the concentration–time curve from 0 to 12 h was 2.58‐fold greater (p < 0.05), whereas the antihyperglycaemic effects of metformin were decreased. The mean differences (90% confidence interval) in mean and maximum serum glucose concentrations and in 2‐h‐post‐OGTT serum glucose concentration were −0.6 (−1, −0.2), −0.9 (−1.6, −0.3) and −0.5 (−1.1, 0.1) mmol/l, respectively. These findings indicate that the response to metformin is not only related to the plasma exposure of metformin but is also related to other factors, such as inhibition of uptake transporters and the gastrointestinal‐based pharmacology of metformin.</P>

알파 방사선에 의한 국민위해평가(Ⅳ)

이해룡,오헌진,이광용,김혁주,오현주,김지순,김철현,정진백,전재식,김형국 국립보건연구원 1994 국립보건원보 Vol.31 No.2

PHF2 histone demethylase acts as a tumor suppressor in association with p53 in cancer

Lee, K-H,Park, J-W,Sung, H-S,Choi, Y-J,Kim, W H,Lee, H S,Chung, H-J,Shin, H-W,Cho, C-H,Kim, T-Y,Li, S-H,Youn, H-D,Kim, S J,Chun, Y-S Macmillan Publishers Limited 2015 Oncogene Vol.34 No.22

Plant homeodomain finger 2 (PHF2) has a role in epigenetic regulation of gene expression by demethylating H3K9-Me2. Several genome-wide studies have demonstrated that the chromosomal region including the PHF2 gene is often deleted in some cancers including colorectal cancer, and this finding encouraged us to investigate the tumor suppressive role of PHF2. As p53 is a critical tumor suppressor in colon cancer, we tested the possibility that PHF2 is an epigenetic regulator of p53. PHF2 was associated with p53, and thereby, promoted p53-driven gene expression in cancer cells under genotoxic stress. PHF2 converted the chromatin that is favorable for transcription by demethylating the repressive H3K9-Me2 mark. In an HCT116 xenograft model, PHF2 was found to be required for the anticancer effects of oxaliplatin and doxorubicin. In PHF2-deficient xenografts, p53 expression was profoundly induced by both drugs, but its downstream product p21 was not, suggesting that p53 cannot be activated in the absence of PHF2. To find clinical evidence about the role of PHF2, we analyzed the expressions of PHF2, p53 and p21 in human colon cancer tissues and adjacent normal tissues from patients. PHF2 was downregulated in cancer tissues and PHF2 correlated with p21 in cancers expressing functional p53. Colon and stomach cancer tissue arrays showed a positive correlation between PHF2 and p21 expressions. Informatics analyses using the Oncomine database also supported our notion that PHF2 is downregulated in colon and stomach cancers. On the basis of these findings, we propose that PHF2 acts as a tumor suppressor in association with p53 in cancer development and ensures p53-mediated cell death in response to chemotherapy.

The evolutionary dynamics of highly pathogenic avian influenza H5N1 in south-central Vietnam reveals multiple clades evolving from Chinese and Cambodian viruses

Nguyen, T.H.,Than, V.T.,Thanh, H.D.,Nguyen, V.Q.,Nguyen, K.H.,Nguyen, D.T.,Park, J.H.,Chung, I.S.,Jeong, D.G.,Chang, K.T.,Oh, T.K.,Kim, W. Pergamon Press 2015 Comparative immunology, microbiology and infectiou Vol.42 No.-

In Vietnam, highly pathogenic avian influenza (HPAI), such as that caused by H5N1 viruses, is the most highly contagious infectious disease that has been affecting domestic poultry in recent years. Vietnam might be an evolutionary hotspot and a potential source of globally pandemic strains. However, few studies have reported viruses circulating in the south-central region of Vietnam. In the present study, 47 H5N1-positive samples were collected from both vaccinated and unvaccinated poultry farms in the South Central Coast region of Vietnam during 2013-2014, and their genetic diversity was analyzed. A common sequence motif for HPAI virus was identified at HA-cleavage sites in all samples: either RERRRKR/G (clades 2.3.2.1c and 2.3.2.1a) or REGRRKKR/G (clade 1.1.2). Phylogenetic analysis of HA genes identified three clades of HPAI H5N1: 1.1.2 (n=1), 2.3.2.1a (n=1), and 2.3.2.1c (n=45). The phylogenetic analysis indicated that these Vietnamese clades may have evolved from Chinese and Cambodian virus clades isolated in 2012-2013 but are less closely related to the clades detected from the Tyva Republic, Bulgaria, Mongolia, Japan, and Korea in 2009-2011. Detection of the coexistence of virus clades 2.3.2.1 and the very virulent 1.1.2 in the south-central regions suggests their local importance and highlights concerns regarding their spread, both northwards and southwards, as well as the potential for reassortment. The obtained data highlight the importance of regular identification of viral evolution and the development and use of region-specific vaccines.