DDC,MEA,WR-2721의 放射線 防護效果에 關한 硏究

柳星烈,陳秀一,鄭鉉佑,鄭引溶,高柱煥,고경환 대한방사선 방어학회 1986 방사선방어학회지 Vol.11 No.2

放射線障害 豫防藥劑의 人體適用은 副作用으로 臨床的 利用이 되지 못한 實情으로서 生理學的 및 藥理學的 毒性, 物理學的 諸性質, 藥劑의 作用機轉究明 및 臨床的 技術開發이 未洽하여 放射線 障害者에 對한 治療는 對症療法에 不過한 實情이다. 著者들은 放射線에 依한 人體障害豫防에 必要한 基礎資料를 얻기 위하여 現在까지 比較的 毒性이 적고 化學的으로 安定하며 藥效가 優秀하다고 알려진 DDC, MEA, WR-2721을 選定하여 實驗動物 非近交系 Swiss Webster(NIH-GP)마우스에 利用한 放射線 防護作用에 關한 實驗結果는 다음과 같다. 1) 各種 放射線 防護劑(DDC, MEA, WR-2721)의 마우스에 대한 適定 投與量은 各各 DDC; 1,000, MEA; 200, WR-2721; 400mg/kg이었다. 2) 實驗動物에 放射線 反致死線量 LD50/10에 對한 藥劑의 防護效果을SMS DDC;1.2, MEA; 1.4, WR-2721; 1.9이었고 LD50/30에 對 한 DMF는 DDC; 1.7, MEA; 1.8, WR-2721; 2.5이었다. 3) 空腹 小?腺(Jejunum crypt)의 放射線平均致死線量(Do)에 對한 防護效果 DMF는 DCC; 1.07, MEA; 1.21, WR-2721; 1.76이었고, 小?腺細胞의 致死線 平均致死線量에 對한 DMF는 DDC; 1.04, MEA; 1.08, WR-2721; 1.38이었다. 4) 以上으로 上記 3種의 放射線 防護劑中 WR-2721의 放射線 防護效果가 가장 優秀하였고 이 結果는 向後 臨床的 適用에 關한 硏究의 基礎資料가 될 것으로 思料된다. At present, the treatments of the radiation-induced diseases are only performing by the palliative treatment technique. Moreover, radiation protective agents are a little toxic for human being and this seriously limits their applicability with various complications in clinical uses. Accordingly, as a part of the aim of gain of the basic data for protective roles of some radioprotectors, the present investingation was carried out to evaluate the comparative radioprotective effects by the administration of DDC, MEA, WR-2721. Results are shown for statistically significant analysis and correlation with each group as follows; 1. The proper doses of the radioprotectors were DDC; 1,550mg/kg, MEA; 450mg/kg, WR-2721; 780mg/kg of the mouse body weight. 2. DMF(Dose modification factor) of LD50/10 and LD50/30 for whole body irradiation was DDC; 1.2, MEA; 1.4, WR-2721; 1.9 and DDC; 1.7, MEA; 1.8 and WR-2721; 2.5 respectively. 3. DMF for radiation reaction of jejunal crypt was DDC; 1.07, MEA; 1.21 and WR-2721; 1.76 and that of jejunal crypt cell was DDC; 1.04, MEA; 1.08 and WR-2721; 1.38 respectively. 4. Conclusively, WR-2721 was the most effective drung among the three radioprotectors and this result must be a supportive data for further study for clinical application.

한국에서 분리된 Helicobacter pylori 균주에서의 병리 인자 분포 및 이에 대한 숙주 방어 기전의 분자생물학적 연구

김정목(Jung Mogg Kim),정현채(Hyun Chae Jung),이주영(Joo Young Lee),이경미(Kyung Mee Lee),진영주(Young Joo Chin),김영전(Young Jeon Kim),송인성(In Sung Song),김정룡(Chung Yong Kim) 대한내과학회 1998 대한내과학회지 Vol.54 No.1

Objectives: CagA or cytotoxin-positive H. pylori may be associated with gastroduodenal diseases. However, controversies about this association also exist. Moreover, there could be geographic differences in the prevalence of virulence factors such as cagA or cytotoxin. In H. pylori infection, the gastric mucosa shows acute and chronic inflammation. However, the pathogenesis of such as an inflammation by H. pylori is not well elucidated. We performed this study 1) to determine prevalence of the genes of virulence factor such as cagA and cytotoxin in H. pylori, 2) to assess the correlation of their presence with clinical findings, and 3) to test whether the vacuolating cytotoxin of H. pylori could evoke proinflammatory cytokine gene expression in gastric epithelial cells. Methods: 1) The prevalence of the cagA, vacA and adhesin genes in H. pylori strains isolated from Koreans was determined by PCR analysis. 2) H. pylori was cultured in Brucella broth containing 10% fetal bovine serum for 3 days using a shaker in a microaerophilic condition. Cytotoxin assay was performed by determining whether addition of the concentrated culture supernatants is able to cause vacuolization of HeLa cells. 3) After human gastric epithelial cells, Hs746T and AGS were incubated with the culture supernatants containing vacuolating cytotoxin, each RNAs were extracted from the gastric epithelial cells. And then various cytokine gene expression were assessed using RT-PCR. The expressed cytokine transcripts were quantified by RT-PCR and standard synthetic RNA. Among cytokines, IL-8 proteins were also measured by ELISA. Results: 1) More than 95% of H. pylori isolates from Korean adults possessed cagA, vacA and adhesin genes. And 80.6% of H. pylori strains have expressed vacuolating cytotoxicity against HeLa cells within 24 hours. 2) There was no correlation between the virulence factors of H. pylori strains and clinical findings. 3) Cytotoxin-positive culture supernatants also caused vacuolization in gastric epithelial cells, both Hs746T and AGS. 4) Expression of mRNA for proinflammatory cytokines such as IL-1 α, IL-8, MCP-1 and GM-CSF was much more upregulated by vacuolating cytotoxin-positive culture supernatants than cytotoxin-negative ones in both Hs746T and AGS cells. Number of molecules of the expressed IL-8 transcripts was parallel to the amounts of IL-8 protein secreted from gastric epithelial cells. Conclusion : These results suggest that virulence factors of H. pylori may not be factors determining disease entitiy in Korean patients infected with H. pylori. In addition, vacuolating cytotoxin secreted from H. pylori could give rise to vacuolization in gastric epithelial cells as well as induce proinflammatory cytokines from the cells.

췌관협착을 동반한 만성 췌장염에서 췌관 스텐트 배액술의 임상적 효과

노임환,진영주,송일한,임창영,김정원,김정택 大韓消化器病學會 1998 大韓消化器病學會誌 Vol.32 No.2

궤양성 대장염 환자에서 치료 경과에 따른 대장점막에서의 CXC와 CC Chemokine 유전자의 발현양상

송인성,정현채,이경미,장동경,정운태,진영주,김점룡 대한소화기학회 1998 대한소화기학회지 Vol.31 No.2

Background/Aims: Cytokines play an important role in the recruitment of neutrophil and monocyte. Expression of mRNA for chemokines such as interleukin-8 (IL-8) and monocyte chernoattractant protein-1 (MCP-1) are known to be increased in the colonic mucosa of ulcerative colitis. However, profiles of other recently defined chemokines such as epithelial cell-derived neutrophilactivating peptide-78 (ENA-78) and RANTES (regulated on activation, normal T expressed and secreted) remain to be extensive]y studied. Methods: Total tissue RNA was extracted from colonoscopic biopsies from normal controls (NC, n=10) and patients with active ulcerative colitis (n=17)[from lesion (UCal, n=17) and normal looking mucosa (UCan, n=14)areas] and remissioned ulcerative colitis (n=17)[from lesion (UCrl, n=17) and normal looking mucosa (UCrn, n=12) areas]. The chemokines for the present study were IL-8, MCP-1, ENA-78 and RANTES. Detection of chemokine gene expression was performed by reverse transcriptional PCR. Results: The expression of IL-8 mRNA was increased in UCal than in others (UCan, UCrl, UCrn and NC, p$lt;0.05). The expressions of ENA-78 mRNA were significantly increased in all these 4 groups (UCal, UCrl, UCan and UCrn) than NC (p$lt;0.001) The expression of MCP-1 mRNA was significant]y increased in both UCal and UCrl than its normal looking mucosa respectively and NC (p$lt;0.05). The high expression of RANTES mRNA was noted in all groups (NC, UCal, UCan, UCrl, UCrn)(82%-100%), but there was no statistical significance arnong them. Conclusions: These results suggest that the role of IL-8 confines to only acute inflammed lesion, MCP-1 and ENA-78 play an important role in both acute and remissioned inflammed areas, but the area of expression of ENA-78 was broader than MCP-1. RANTES seems to play an important role in the basic immune system.

Changes in Cecal Microbiota and Short-chain Fatty Acid During Lifespan of the Rat

( Soo In Choi ),( Joo Hee Son ),( Nayoung Kim ),( Yong Sung Kim ),( Ryoung Hee Nam ),( Ji Hyun Park ),( Chin-hee Song ),( Jeong Eun Yu ),( Dong Ho Lee ),( Kichul Yoon ),( Huitae Min ),( Yeon-ran Kim ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2021 Journal of Neurogastroenterology and Motility (JNM Vol.27 No.1

Background/Aims The gut microbiota regulates intestinal immune homeostasis through host-microbiota interactions. Multiple factors affect the gut microbiota, including age, sex, diet, and use of drugs. In addition, information on gut microbiota differs depending on the samples. The aim of this study is to investigate whether changes in cecal microbiota depend on aging. Methods Gut microbiota in cecal contents of 6-, 31-, and 74-week-old and 2-year-old male Fischer-344 rats (corresponding to 5-, 30-, 60-, and 80-year-old humans in terms of age) were analyzed using 16S ribosomal RNA metagenome sequencing and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) based on the Kyoto Encyclopedia of Genes and Genomes orthology. Moreover, short-chain fatty acid (SCFA) level in cecum and inflammation related factors were measured using real-time quantitative polymerase chain reaction and enzyme linked immunosorbent assay. Results Alpha and beta diversity did not change significantly with age. At the family level, Lachnospiraceae and Ruminococcaceae, which produce SCFAs, showed significant change in 31-week-old rats: Lachnospiraceae significantly increased at 31 weeks of age, compared to other age groups, while Ruminococcaceae decreased. Butyrate levels in cecum were significantly increased in 31-week-old rats, and the expression of inflammation related genes was increased followed aging. Especially, EU622775_s and EU622773_s, which were highly abundance species in 31-week-old rats, showed significant relationship with butyrate concentration. Enzymes required for producing butyrate―acetyl-CoA transferase, butyryl-CoA dehydrogenase, and butyrate kinase―were not predicted by PICRUSt. Conclusions Major bacterial taxa in the cecal lumen, such as Lachnospiraceae, well-known SCFAs-producing family, changed in 31-week-old rats. Moreover, unknown species EU622775_s and EU622773_s showed strong association with cecal butyrate level at 31 weeks of age. (J Neurogastroenterol Motil 2021;27:134-146)

궤양성 대장염 환자에서 치료 경과에 따른 대장점막에서의 CXC와 CC Chemokine 유전자의 발현양상

이경미,진영주,장동경,정운태,정현채,송인성,김정룡 大韓消化器病學會 1998 大韓消化器病學會誌 Vol.31 No.2

이유기 흰쥐 소장 점막 유당분해효소에 대한 갑상선호르몬의 영향

김태호,이동호,송인성,정현채,이국래,김정용,장동경,진영주 대한소화기학회 1998 대한소화기학회지 Vol.31 No.6

Background/Aims: The decrease in intestinal lactase activity goes inversely with the increase in thyroid hormone level during weaning period of rat. The aim of this study is to identify whether increase in the concentration of thyroid hormone in blood before weaning period can accelerate the dechne of lactase activity and to explore its genetic regulation. Methods: We supplied triiodothyronin (30 ㎍/100 g body weight/day in 1 ml of NaOH) far 36 of 8-day-old (day 8) Sprague-Dawley rats intraperitoneally for 2 weeks (T₃ group) and 1 ml of 0.01 M NaOH for the 42 controls. We quantified setum T₃, mucosal lactase and sucrase activity, and lactase mRNA using cDNA probe pRLU6 on the days 8, 9, 12, 15, 18, 21 and 26 which was 5 days after the end of the injection. Results: Intestinal lactase activities were 101, 83, 84, 72, 75, 52, and 17 mu/mg protein on the days 8, 9, 12, 15, 18, 21, and 26 in the control group, whereas 72, 58, 50, 28, 21, and 19 mu/mg protein on the days 9, 12, 15, 18, 21, and 26 in the T₃ group. Decline of lactase activities in the T₃ group was earlier and more steady than that in the control group. The lactase activities were not correlated with lactase mRNA levels. Sucrase activities in the T3 group were 6, 17, 32, 47, 60, and 52 mu/mg protein on the days 9, 12, 15, 18, 21, and 26, whereas those in control group did not approach 9 mu/mg protein until the day 18. Conclusions: Early increased thyroid hormone can accelerate the change of lactase and sucrase activities during weaning petiod of rats. In addition, the decrease in lactase activities was not accompanied by the decrease in mRNA levels. These results suggest that the genetic regulation of lactase activities occur at the post-transcriptional level.

이유기 흰쥐 소장 점막 유당분해효소에 대한 갑상선호르몬의 영향

장동경,진영주,이국래,김태호,이동호,정현채,송인성,김정룡 大韓消化器病學會 1998 大韓消化器病學會誌 Vol.31 No.6

결핵성 대장염 환자의 대장병변에서 치료경과에 따른 Chemokine 유전자의 발현양상

정운태,장동경,이경미,진영주,김태호,이동호,정현채,송인성,김정룡 大韓消化器病學會 1998 大韓消化器病學會誌 Vol.31 No.6

문맥압항진증에 의한 식도정맥류와 울혈성 위병변에서 Inducible Nitric Oxide Synthase mRNA 발현양상

송일한,최정,진영주,임창영,김정원,노임환 大韓消化器病學會 1998 大韓消化器病學會誌 Vol.32 No.2