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Toluene, Xylene, Trichloroethylene 투여가 흰쥐의 시상하부와 뇌하수체의 GnRH, GnRH Receptor, Pit-1 유전자 발현에 미치는 영향
기노석,이종태,강대성,김대환,이채관,이헌,전진호,김정호,이채언 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.2
The workplace exposure of chemicals has steadily increased, therefore the concern for subsequent effect on reproductive outcome has been an important issue in occupational medicine. In previous studies, higher rates of spontaneous abortion, reduced fertility and menstrual disorder among women, and an impairement of sperm quantity and quality among men have been associated with a wide variety of chemical agents. This study was conducted to evaluate the effects of toluene, xylene and trichloroethylene(TCE) injection on the mRNA levels of GnRH, GnRH receptor and Pit-1 genes in male rats hypothalamus and pituitary and the effects on the plasma levels of FSH, LH, prolactin and testosterone. Sprague-Dawley male rats were divided into five groups of five each according to concentration of toluene, xylene and TCE. The rats were injected subcutaneously to 0, 50, 100, 200, 400 mg/kg body weight/day of toluene, xylene and TCE, respectively for 6 days. Rat brains were excised and hypothalamus and pituitary were separated. Reverse transcription-polymerase chain reaction (RT-PCR) and RNase protection assay(RPA) were used to evaluate the GnRH, GnRH receptor and Pit-1 mRNA levels. Plasma concentrations of FSH, LH, prolactin and testosterone were assayed by radioimmunoassay(RIA). The results were as follows; 1. GnRH, GnRH receptor and Pit-1 mRNA levels in toluene and xylene injected groups, and GnRH receptor mRNA levels in TCE injected group were lowered dose-dependently. Especially, GnRH receptor and Pit-1 mRNA levels in 200mg/kg of toluene injected group, and GnRH, GnRH receptor and Pit-1 mRNA levels in 400mg/kg of toluene injected group were significantly lower than control group (p<0.05). GnRH receptor and Pit-1 mRNA levels in 400mg/kg of xylene injected group, and GnRH receptor mRNA levels in 400mg/kg of TCE injected group were significantly lower than control group(p<0.05). 2. The plasma levels of prolactin and testosterone in 400mg/kg of toluene injected group, and LH in 100, 200 and 400mg/kg of xylene injected group, and testosterone in 400 mg/kg of TCE injected group were significantly lower than control group(p<0.05). In conclusion, we speculated that toluene and xylene affected reproductive system secondarily through hypothalamus-pituitary axis, and TCE affected directly through steroidogenesis. And we recommended that further study for assessment of the reproductive toxicity of mixed organic solvent exposures should be conducted.
Lee, Seung Hun,Jeong, Myung Ho,Kim, Ju Han,Kim, Min Chul,Sim, Doo Sun,Hong, Young Joon,Ahn, Youngkeun,Chae, Shung Chull,Seong, In Whan,Park, Jong Sun,Chae, Jei Keon,Hur, Seung Ho,Cha, Kwang-Soo,Kim, H Elsevier 2018 Journal of cardiology Vol.72 No.5
<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Ventricular tachycardia or fibrillation (VT/VF) is a major cause of sudden cardiac death after acute myocardial infarction (AMI). This study aims to investigate the clinical characteristics and outcomes of VT/VF, to identify the variables associated with VT/VF, and to construct a new scoring system.</P> <P><B>Methods</B></P> <P>Patients with relatively preserved left ventricular ejection fraction (LVEF) (≥40%) included in the Korea Acute Myocardial Infarction Registry-National Institutes of Health registry were enrolled in this study. Among 13,109 patients in the registry, a total of 10,334 (78.8%) had relatively preserved LVEF after AMI. Patients were divided into two groups based on whether they experienced life-threatening VT/VF during hospitalization or not. The predictors for VT/VF during hospitalization were assessed. In-hospital mortality and complications were recorded.</P> <P><B>Results</B></P> <P>A total of 358 (3.5%) experienced life-threatening VT/VF. The VT/VF group was at an increased risk of in-hospital mortality (odds ratio 2.99) and cardiac death (odds ratio 3.40). Variables of diagnosis, Killip class, smoking, initial rhythm, left bundle branch block, and LVEF were significant indicators of VT/VF. A new risk score system yielded acceptable discrimination function (<I>c</I>-statistics=0.773).</P> <P><B>Conclusions</B></P> <P>Relatively preserved LVEF patients could still be at risk of life-threatening VT/VF, which is related to a poor prognosis during the admission period. This new scoring system can be adopted to stratify the risk of VT/VF.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 3.5% of acute myocardial infarction patients experienced ventricular tachycardia or fibrillation (VT/VF) even if their left ventricular ejection fraction was preserved. </LI> <LI> Early VT/VF during hospitalization was associated with a poor prognosis. </LI> <LI> The VAMIR score could predict VT/VF, and it yielded acceptable discriminant function. </LI> <LI> The VAMIR score supported the strategies of earlier discharge after revascularization. </LI> </UL> </P>
Lee, Jinwoo,Na, Hyon Bin,Kim, Byoung Chan,Lee, Jin Hyung,Lee, Byoungsoo,Kwak, Ja Hun,Hwang, Yosun,Park, Je-Geun,Gu, Man Bock,Kim, Jaeyun,Joo, Jin,Shin, Chae-Ho,Grate, Jay W.,Hyeon, Taeghwan,Kim, Jungb Royal Society of Chemistry 2009 Journal of materials chemistry Vol.19 No.42
<P>A magnetically-separable and highly-stable enzyme system was developed by adsorption of enzymes in superparamagnetic hierarchically ordered mesocellular mesoporous silica (M-HMMS) and subsequent enzyme crosslinking. Superparamagnetic nanoparticles were homogeneously incorporated into hierarchically-ordered mesocellular mesoporous silica (HMMS) by the decomposition of a preformed iron propionate complex. The size of the incorporated superparamagnetic nanoparticles was around 5 nm, generating a magnetically separable host with high pore volumes and large pores (M-HMMS). α-chymotrypsin (CT) was adsorbed into M-HMMS with high loading (∼30 wt%) in less than 30 minutes. Glutaraldehyde (GA) treatment of adsorbed CT resulted in nanometer scale crosslinked enzyme aggregates in M-HMMS (CLEA-M). The activity of these CT aggregates in M-HMMS (CLEA-M-CT) was 34 times than that of simply adsorbed CT in M-HMMS, due to an effective prevention of enzyme leaching during washing <I>via</I> a ship-in-a-bottle approach. CLEA-M-CT maintained the initial activity not only under shaking (250 rpm) for 30 days, but also under recycled uses of 35 times. The same approach was employed for the synthesis of CLEA-M of lipase (CLEA-M-LP), and proven to be effective in improving the loading, activity, and stability of enzyme when compared to those of adsorbed LP in M-HMMS.</P> <P>Graphic Abstract</P><P>Crosslinked enzyme aggregates in magnetitie-coated mesoporous silica, fabricated <I>via</I> a two-step procedure of enzyme adsorption and crosslinking, were highly active, highly stable, and easily recyclable using a magnet. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b909109b'> </P>
Intravesical bacillus Calmette–Guérin-induced myopathy presenting as rhabdomyolysis: a case report
Chae Hun Lee,Byeong Joo Choi,Jung Hun Kim,Tae Woong Yang,Gi Jeong Kim,Ha Young Shin,Se Hoon Kim,Seung Woo Kim 영남대학교 의과대학 2023 Yeungnam University Journal of Medicine Vol.40 No.4
Intravesical bacillus Calmette–Guérin (BCG) instillation is an adjuvant treatment for non–muscle-invasive urinary bladder cancer. Although most complications associated with BCG immunotherapy are mild and self-limiting, rare albeit serious complications have been reported. Only a few cases of BCG-related rhabdomyolysis have been reported. In this study, we present the case of a 72-year-old woman who developed severe weakness and hyperCKemia following intravesical BCG instillation. A muscle biopsy was performed, and a diagnosis of drug-induced myopathy was made.
Lee, Seung-Hun,Kim, Ha-Young,Lee, Haeseung,Kim, Jong Wan,Lee, Yu-Ran,Chae, Myeong Ju,Oh, Sang-Ik,Kim, Jong Ho,Rhee, Man Hee,Kwon, Oh-Deog,Goo, Youn-Kyoung,Kim, Tae-Hwan,Geraldino, Paul John L,Kwak, Do BMJ Group Group Ltd 2018 The Veterinary record Vol.183 No.16
<P>This study evaluated the prevalence of <I>Eimeria</I> species, particularly <I>E bovis</I>, <I>E zuernii</I> and <I>E auburnensis</I> that are pathogenic to cattle, in faecal samples collected from cattle with diarrhoea reared in the Republic of Korea by using microscopy and PCR. In addition, the prevalence of <I>Eimeria</I> species was analysed according to age, type of cattle, region, season and nature of diarrhoea. Overall, <I>Eimeria</I> species were identified in 279 of the 1261 (22.1 per cent) faecal samples through microscopy, and statistical analysis revealed a lower prevalence in calves aged than three weeks or less and higher prevalence in cattle with haemorrhagic diarrhoea. Of the 279 microscopy-positive samples, <I>E bovis</I>, <I>E zuernii</I> and <I>E auburnensis</I> were identified in 100 (7.9 per cent), 83 (6.6 per cent) and 27 (2.1 per cent) faecal samples, respectively, by using PCR. To the authors' knowledge, this study is the first to apply PCR for epizootiology of bovine coccidiosis.</P>