Antibiotic susceptibility and resistance of Streptococcus iniae and Streptococcus parauberis isolated from olive flounder (Paralichthys olivaceus)

Park, Y.K.,Nho, S.W.,Shin, G.W.,Park, S.B.,Jang, H.B.,Cha, I.S.,Ha, M.A.,Kim, Y.R.,Dalvi, R.S.,Kang, B.J.,Jung, T.S. Elsevier Scientific Pub. Co 2009 Veterinary microbiology Vol.136 No.1

The rates of antibiotic susceptibility and resistance were investigated in Streptococcus iniae and Streptococcus parauberis isolates obtained from diseased olive flounders (Paralichthys olivaceus) collected from fish farms in Jeju Island, Korea. Isolates of S. iniae (n=65) were susceptible to cefotaxime, erythromycin, ofloxacin, penicillin, tetracycline and vancomycin, as demonstrated by the minimum inhibitory concentration (MIC) test. Isolates of S. parauberis (n=86) were highly resistant to erythromycin (58% of the 86 isolates tested) and tetracycline (63% of the 86 isolates tested). Fifty-four isolates of tetracycline-resistant S. parauberis contained the tet(M/O/S) genes, of which 39 and 12 isolates contained the tet(M) and tet(S) genes, respectively, whereas 3 isolates contained both the tet(M) and tet(S) genes. Among the erythromycin-resistant isolates of S. parauberis (n=50) only 14 contained the erm(B) gene. These results suggest that the tet(S) and erm(B) genes of S. parauberis are involved in the acquisition of high-level resistance to erythromycin and tetracycline. Our findings reveal a high rate of antibiotic resistance among strains of S. parauberis and emphasize the need to develop an appropriate vaccine to reduce the use of antibiotics.

인두편도염 환자와 보균자에서 분리한 Streptococcus pyogenes의 T-protein serotyping 및 항균제 감수성

이영희,황규잠,주영란,박강수,이광준,엄영길,배송미,차성호 국립보건연구원 1998 국립보건원보 Vol.35 No.-

대학생의 아이돌 애착 경험

강민지,구영민,박소진,박수빈,박혜원,원유영,차민희,김석선,이미선 이화여자대학교 간호학회 2018 이화간호학회지 Vol.- No.52

Purpose: The purpose of this study was to obtain comprehensive understanding of Korean university students’; experience having attachment to Idol. The findings of this study will help find nursing method to improve quality of mental health of university students. Method: Max van Manen’s hermeneutical phenomenology was adopted for the study. 8 participants were recruited using Random Sampling method: aged from 17 to 24 years old. Data came from in-depth, semi-structured interviews. Max van Manen’s 4 steps method for data analyzing were adopted. Result: The analysis produced five themes: 'Fall into Idol for the first time', 'Affection that infinitely penetrates one's heart', 'Secure attachment towards Idol leads to the route of happiness', 'Exhausted lives due to Insecure attachment towards Idol', 'Idol, who is actually in the other world' Conclusion: These results presented that university students could gain psychological stability through fan activity, and consider it as a leisure activity. Meanwhile, they spent a lot of money and time due to an obsession with Idol, and felt negative emotions like skepticism or isolation because of the one-way relationship with Idol. Thus, Mental health professionals need to encourage university students who are attached to Idol to build relationships based on reality, and provide mental health counseling to help them handle emotional stress.

In vitro and in vivo antifungal activities of decursin and decursinol angelate isolated from Angelica gigas against Magnaporthe oryzae, the causal agent of rice blast

Yoon, M.Y.,Kim, Y.S.,Ryu, S.Y.,Choi, G.J.,Choi, Y.H.,Jang, K.S.,Cha, B.,Han, S.S.,Kim, J.C. Academic Press 2011 Pesticide biochemistry and physiology Vol.101 No.2

Blast is considered the most important fungal disease of rice due to its wide distribution and destructiveness under favorable conditions. Development of new effective and environmentally benign agents against the causal pathogen, Magnaporthe oryzae, is of great interest. In the course of a search for natural antifungal compounds in medicinal plants, we found that the methanol extract of Angelica gigas roots showed a potent control efficacy against rice blast caused by M. oryzae. We isolated antifungal coumarins from the extract, and they were identified as decursin and decursinol angelate. Antifungal activities of these compounds, along with kasugamycin, were tested on M. oryzae in vivo and in vitro. In an in vivo assay, the three compounds effectively suppressed the development of rice blast at concentrations more than 100μg/mL. Coumarins showed relatively weak inhibitory effect on fungal mycelial growth when compared to kasugamycin. However, they strongly inhibited M. oryzae spore germination, which was not observed in kasugamycin treatments. This is the first report demonstrating that decursinol angelate can provide control against rice blast and that the two coumarins inhibit M. oryzae spore germination. In addition, the wettable powder formulation of the crude extract of A. gigas prohibited the development of blast symptoms on rice plants more effectively than liquid concentrate formulation of kasugamin, a commercial fungicide. Based on our study, we propose that coumarin compounds as well as the A. gigas root crude extract can be used as natural, benign fungicides for controlling rice blast.

Inflammatory lipid sphingosine-1-phosphate upregulates C-reactive protein via C/EBPβ and potentiates breast cancer progression

Kim, E-S,Cha, Y,Ham, M,Jung, J,Kim, S G,Hwang, S,Kleemann, R,Moon, A Macmillan Publishers Limited 2014 Oncogene Vol.33 No.27

A crucial role of the inflammatory lipid sphingosine-1-phosphate (S1P) in breast cancer aggressiveness has been reported. Recent clinical studies have suggested that C-reactive protein (CRP) has a role in breast cancer development. However, limited information is available on the molecular basis for the expression of CRP and its functional significance in breast cell invasion. The present study aimed to elucidate the molecular link between S1P and CRP during the invasive process of breast epithelial cells. This is the first report showing that transcription of CRP was markedly activated by S1P in breast cells. Our data suggest that not only S1P treatment but also the endogenously produced S1P may upregulate CRP in breast carcinoma cells. Transcription factors CCAAT/enhancer-binding protein beta and c-fos were required for S1P-induced CRP expression. Coupling of S1P<SUB>3</SUB> to heterotrimeric G<SUB>αq</SUB> triggered the expression of CRP, utilizing signaling pathways involving reactive oxygen species (ROS), Ca<SUP>2+</SUP> and extracellular signal-related kinases (ERKs). S1P-induced CRP expression was crucial for the transcriptional activation of matrix metalloproteinase-9 through ERKs, ROS and c-fos, leading to breast cell invasion. Using a xenograft mice tumor model, we demonstrated that S1P induced CRP expression both in vitro and in vivo. Taken together, our findings have revealed a molecular basis for S1P-induced transcriptional activation of CRP and its functional significance in the acquisition of the invasive phenotype of human breast epithelial cells under inflammatory conditions. Our findings may provide useful information on the identification of useful therapeutic targets for inflammatory breast cancer.

M-ary SS 시스템을 활용한 고속 전력선 통신 성능에 관한 연구

문경환(K.H. Moon),신명철(M.C. Shin),서희석(H.S. Seo),최상열(S.Y. Choi),최인혁(I.H. Choi),김학만(H.M. Kim),허남영(N.Y. Hur),차재상(J.S. Cha) 대한전기학회 2004 대한전기학회 학술대회 논문집 Vol.2004 No.7

Prevalence and antimicrobial susceptibility of Salmonella isolates in Pekin ducks from South Korea

Cha, S.Y.,Kang, M.,Yoon, R.H.,Park, C.K.,Moon, O.K.,Jang, H.K. Pergamon Press 2013 Comparative immunology, microbiology and infectiou Vol.36 No.5

An investigation was carried out to determine the prevalence and antibiotic resistance of Salmonella serotypes at South Korean duck farms. A total of 7119 samples collected from 72 duck farms in five provinces were examined from 2011 to 2012. The overall prevalence of Salmonella serotypes was 43.4% (69/159) in duck flocks from 65.2% (47/72) of the duck farms. Eighty-five strains were isolated from 69 duck flocks. Three serotypes of Salmonella enterica were identified such as S. Typhimurium (39/85), S. Enteritidis (44/85), and S. London (2/85). The prevalence of Salmonella infection decreased significantly in 3-week-old ducks compared to that in 1-week-old ducks (P<0.05). All isolates except one were resistant to at least one antimicrobial and 27% of the isolates were resistant to 5-16 antimicrobials. Our findings provide baseline information on the degree of Salmonella infection and distribution of Salmonella serotypes in ducks and indicate that ducks should be considered an important source of foodborne pathogens.

Plasma Ghrelin Levels and Their Relationship with Gastric Emptying in Patients with Dysmotility-Like Functional Dyspepsia

Lee, K.J.,Cha, D.Y.,Cheon, S.J.,Yeo, M.,Cho, S.W. S. Karger AG 2009 Digestion Vol.80 No.1

<P><I>Background/Aims:</I> Ghrelin affects gastric motility. However, little is known about the role of ghrelin in the pathophysiology of functional dyspepsia (FD). We investigated plasma ghrelin levels and their relationship with gastric emptying time in dysmotility-like FD patients. <I>Methods:</I> 42 patients with dysmotility-like FD and 14 healthy volunteers were enrolled in the study. Gastric half-emptying time was measured using a radiolabeled meal. Plasma total ghrelin levels before and after the meal were determined using a radioimmunoassay kit. <I>Results:</I> Preprandial ghrelin levels were significantly lower in FD patients than in controls. Postprandial ghrelin levels were similar between the two groups. Abnormally low preprandial ghrelin levels were observed in 7 out of 42 patients, in whom significant postprandial decrease of ghrelin levels was absent. Delayed gastric emptying was observed in 5 out of 7 patients with abnormally low ghrelin levels. Pre- or postprandial ghrelin levels were not significantly correlated with gastric half-emptying time, both in the patient group and in the control group. <I>Conclusions:</I> Abnormally low preprandial ghrelin levels and absence of significant postprandial decrease of ghrelin levels are present in a subset of dysmotility-like FD patients. Further investigation on the pathogenetic implication of these alterations in FD is required.</P><P>Copyright © 2009 S. Karger AG, Basel</P>

부산지역 약물남용환자 실태에 관한 조사연구

차영미,김공현,윤용균,진성태 인제대학교 1992 仁濟醫學 Vol.13 No.1

본 조사연구는 1988년 7월 1일부터 1990년 6월 38일까지 2년 간에 걸쳐 지방공사 부산의료원에서 약물남용환자로 입원하여 치료받고 퇴원한 전체 351명(남자 263명, 여자 88명)을 대상으로 이들의 치료 경과를 기록해 놓은 신경정신과의 병록지를 중심으로 부산지역 약물남용환자들의 실태를 파악한 것이다. This study was carried out to identify characteristics of drug abusers who were treated and discharged from 1 July 1988 to 30 June 1990 in the Department of Neuropsychiatry, the Pusan Municipal Medical Centre. The subjects were composed of 351 patients who were discharged during the period of the study. Data for the study were obtained from the subjects medical records of the Centre The results are as follows : 1.40.2% of the subjects were in the 30-39 age group and 77.8% of them were in the 20's and 30's. 2.90.9% of the subjects exposed to narcotics at ages of 19-39. It is observed that there is an increasing tendency of drug abuse in teen-agers. 3.82.3% of the subjects were exposed to natorics by either friends or peers. 4.70.0% of the subjects agreed that the primary motive to take narcotics was inducement or curiosity. 5.76.3% of the subjects took narcotics 1 or 2 times per day, 15.6% 3 times, 4.2% 4 times, 2.4% 5 times. 6.70.0% of the subjects bought narcotics from peddlers, 29.7% from dens of addicts, only 0.3% from pharmacy.

장티프스에서 효소면역측정법의 진단적 가치

차창용,김윤원,황응수,정문현,오명돈,최강원 대한감염학회 1985 감염 Vol.17 No.2

The diagnostic value of ELISA for typhoid fever was tested with 43 sera from patients with enteric fever and 27 sera from patient who had no known history of enteric fever. IgG antibody was most commonly elevated (in 97.6%), followed by IgA (in 38.3%) and IgM antibody (in 65.1%). On the other hand, IgM and IgA antibody were found to be elevated in 40.7% of various infectious (notably due to M, tbc and gram negative enteric bacteria) and noninfectious conditions. On the contrary, IgG antibody was elevated only occasionally. (7.4%). IgG antibody was most sensitive and specific indicator for the S. typhi and S. paratyphi A infection.