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정병룡,박의호 대구대학교 과학기술연구소 1996 科學技術硏究 Vol.3 No.1
Leucaena is the common name for Leucaena leucocephala which is a tropical leguminous tree with a potential value as a source of wood production, high quality forage, and revegetation of hillslopes. In spite of the plant's value, both the differences of pod setting ratio among Leucaena species and the differences of flowering period limited breeding of high quality species of Leucaena plant. To overcome these problems this study was conducted to find the possibility of long term storage of Leucaena pollen by using vacuum and low temperatures. Pollen viability decreased after 1.5 days after storage at room temperature. The same trend was observed for the pollen stored at refrigerator (91℃). Pollen viability, however, was maintained longer with decreasing storage temperature to that of freezer (-17℃). Vacuum treatment also helped to maintain pollen viability either at room or refrigerator temperatures. The ratio of exploded pollen showed the same pattern as the percentage of normally germinated pollen stored under different conditions except the pollen stored at refrigerator. Average tube lengths of germinated pollens were 4.3 and 3.6 mm for the pollen stored at freezer and refrigerator with vacuum, respectively. In conclusion, the viability of Leucaena pollen could be extended by storing under vacuum at freezing temperatures.
신태용,염정열,김원,김현미,박해정,장진이,조성룡,채병숙 우석대학교 생명과학연구소 1998 생명과학연구소 논문집 Vol.2 No.-
We investigated the effect of aqueous extract of Ammomum xanthioides(AXAE) on immediate hypersensitivity. AXAE inhibited systemic anaphylaxis induced by compound 48/80 in mice. AXAE inhibited serum histamine levels induced by compound 48/80 in mice. Moreover, AXAE dose-dependently inhibited histamine release in peritoneal mast cells activated by compound 48/80. These results indicate that the AXAE may be benificial in the regulation of immediate type allergic reaction.
Development of a minipig physical phantom from CT data
Park, Sooyeun,Lee, Pilsoo,Ha, Wi-Ho,Kim, Han Sung,Park, Byeong Ryong,Kim, Jae ,Seok,Shim, Sehwan,Park, Sunhoo,Kim, Young-su,Kim, Chan Hyeong,Jin, Young-Woo JAPAN RADIATION RESEACH SOCIETY 2017 JOURNAL OF RADIATION RESEARCH Vol.58 No.5
<P><B>ABSTRACT</B></P><P>Quantification of pathological progression of radiation-induced injury is essential in development of treatment methods, and a proper animal model is necessary for relevant radiological and medical studies. A minipig is a current animal model selected because of its similarities to humans in anatomy and pathology. In the present study, a minipig physical phantom was developed using computed tomography (CT) data. For dosimetry purposes, the minipig physical phantom was constructed on a slice-by-slice basis, with an array of holes to accommodate dosimeters. The phantom is constituted of three major organs, i.e. bone, lung, and remaining soft tissue, and the organs are clearly distinguishable on each 20-mm-thick axial slice. The quality of the tissue-equivalent (TE) substitutes was analyzed in terms of the atomic compositions and Hounsfield units (HUs). The density (in g/cm<SUP>3</SUP>) and effective atomic number of TE substitutes for the bone, lung, and soft tissue are 1.4 and 7.9, 0.5 and 10.0, and 1.0 and 5.9, respectively. Although the TE substitutes have slightly different physical properties, we think the phantom is acceptable because the HU values of the TE substitutes lie in the HU range of real tissues.</P>
Antimicribial and Antioxidant Activities of Ethanol Extracts of Medicinal Plants
Jeong-Ryong Do,Ki-Ju Kim,Seung-Yong Park,Ok-Hwan Lee,Byeong-Sam Kim,Suk-Nam Kang 한국식품영양과학회 2005 Preventive Nutrition and Food Science Vol.10 No.1
The objective of this study was to determine the radical scavenging activity, total phenolic content, antimicrobial activity, minimum inhibitory concentration (MIC) of ethanol extracts of 32 medical plant species that have been commonly used in medicinal plants. Total phenolic index of T. chebula exhibited the highest value (498.01 mg/g), followed by R. coreanus miquel (400.33 mg/g), Sanguisorba officinalis (368.25 mg/g), P. thumbergiana (259.74 mg/g) and Eugenia aromaticum (229.38 mg/g). Radical scavenging activity for the DPPH radical was highest in T. chebula (40.91%, p<0.01), followed by C. sappan (36.50%), S. officinalis (32.92%), R. coreanus miquel (26.54%) and P. thumbergiana (24.50%). The extracts from T. chebula, R. coreanus muquel, C. sappan, E. aromaticum, S. officinalis and C. japonica possessed outstanding antimicrobial activity against Escherichia coli, Salmonella Typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and Lactobacillus plantarum. MIC was determined on those extracts that showed high efficacy against the test organisms. The most potent MIC values were seen for T. chebula extract against P. aeruginosa, S. aurusa, E. coli, B. subtilis, L. plantarum and S. Typhimurium at 7.8, 7.8, 15.6, 7.8, 125 and 31.2 ㎍/mL, respectivley. Furthermore, the total phenolic content and radical scavenging activity were very closely correlated for all samples (r=0.78). The coefficient correlations between total phenolic index and antimicrobial activity were 0.91 (E. coli), 0.91 (B. subtillis), 0.79 (P. aeruginosa), 0.79 (S. Typhimurium) and 0.70 (L. plantarum).
전병선(Byeong-Seon Jeon),양재원(Jai-Won Yang),박채규(Chae-Kyu Park),고성룡(Sung-Ryong Ko),掘野俊郞(Toshiro Horino),손종록(Jong-Rok Son),박원종(Won-Jong Park) 한국식품영양과학회 1993 한국식품영양과학회지 Vol.22 No.5
인삼의 재배년근별, 국내산지별 및 국가별 무기물의 분포를 조사하기 위하여 시험한 결과 Mn은 2년근에 비하여 5년근에서 4배 가까운 증가추세를 나타내었다. 재배산지별로 볼 때 P은 각 지역별로 큰 차이를 나타내지 않았다. 한편 각국 인삼별로는 중국 전칠삼에서 N의 함량이 적게 나타났으며 P은 한국산 정상흥삼에서 높게 나타났다. Mineral compositions in various ginseng, which was grown in different region, different age, were analyzed by atomic absorption spectrophotometer. Mn^(++) content in ginseng of five years old was four times higher than that of two years old, suggesting that the amount of Mn^(++) would be increased as they are cultivated. The content of phosphate in the various ginseng did not show much difference, whereas that of Ca^(++), Mn^(++) did. Korean red ginseng revealed much higher in Mn^(++) content than that of others investigated. When the amount of N was compared, San Chi, Chinese ginseng was significantly lowered. Korean red ginseng of 100g contains highest phosphate ion of 328.3㎎.
정병룡 ( Byeong-ryong Jeong ),이태이 ( Tae-yi Lee ),박민정 ( Min-jung Park ),하다희 ( Da-hee Ha ),정종배 ( Jong-bae Chung ),이용세 ( Yong-se Lee ) 한국환경농학회 2018 한국환경농학회지 Vol.37 No.1
탄저병이 발생한 자두에서 분리된 C. acutatum 6개와 C.gloeosporioides 5개 균주에 대한 benoomyl, prochloraz manganese complex, azoxystrobin, pyraclostrobin, tebuconazole 및 dithianon의 균사생장억제효과와 포자발아억제효과를 invitro에서 검정하였다. 포장 권장사용농도를 기준으로 비교하면, prochloraz manganese가 탄저병 균주의 균사생장을 가장 효과적으로 억제하였다. 포자발아억제에서는 dithianon과 pyraclostrobin의 효과가 가장 높았다. Benomyl, prochloraz manganese complex, azoxystrobin 및 tebuconazole은 포자의 발아를 억제하지는 않았으나, 발아 후 비정상적인 발아관의 신장을 유기하여 균사생장 억제하였다. 본 연구의 결과를 통해 자두 탄저병에 대하여 dithianon은 예방효과, prochloraz manganese, azoxystrobin, tebuconazole 등은 치료효과, pyraclostrobin은 예방 및 치료효과가 높은 약제로 판단되며, 포장 적용시험을 통해 자두의 탄저병을 효과적으로 방제할 수 있는 약제를 선발해야 할 것이다. BACKGROUND: In order to select a fungicide that can effectively control anthracnose disease in Japanese plum fruit, mycelial growth inhibition effect and spore germination inhibition effect of six fungicides were tested in vitro against six isolates of Colletotrichum acutatum and five isolates of C. gloeosporioides that were isolated from diseased Japanese plum fruit. METHODS AND RESULTS: Inhibitory effects of fungicides on mycelial growth were investigated after inoculating each isolate on potato dextrose agar amended with four discriminatory concentrations of each fungicide for 7 days at 25℃. For spore germination inhibitory effect, each isolate of the Colletotrichum spp. was cultured in potato dextrose agar for 7-14 days at 25°C. After adjusting the concentration of spores of each isolate to 1×10<sup>6</sup> mL<sup>-1</sup> by diluting with 0.025% PDB, the spore suspension was mixed with each fungicide (1:4, v/v), and 60 μL aliquots were dispensed to sterile hole slide glass. Hole slide glasses were placed in a humidified box and incubated for 15 hours at 25°C. Then, spore germination was observed under an optical microscope. At recommended concentration of fungicide prochloraz manganese showed the highest mycelial growth inhibitory effect and dithianon showed the lowest mycelial growth inhibition. The EC50 values for the inhibition of spore germination by dithianon and pyraclostrobin were 0.069-0.126 μg/mL and 0.37-1.59 μ g/mL, respectively. Although benomyl, prochloraz manganese, azoxystrobin, and tebuconazole did not inhibit the spore germination, they appeared to restrain mycelial growth by abnormal growth of germ tube and mycelium after germination. CONCLUSION: Dithianon seemed to have preventive effect. Prochloraz manganese, azoxystrobin, and tebuconazole were likely to have control effect. Pyraclostrobin is considered to have both preventive and control effect against anthracnose disease of Japanese plum fruit.