The whole wheat effect and refined with E-selectin polymorphism on breast cancer

Bilal Fadıl Zakariya,Asmaa M. Salih Almohaidi,Seçil Akilli Şimşek,Areege Mustafa Kamal,Wijdan H. Al-Dabbagh,Safaa A. Al-Waysi 한국분석과학회 2022 분석과학 Vol.35 No.4

Wheat is rich in sources of fiber, oligosaccharides, and resistant starch, simple carbohydrates which may have a protective role against carcinoma. Additionally, Whole wheat/bran as well includes contains phytochemicals such as flavonoids, lignans, folate, phytosterols, phenolic acids, and tocols. The above phytochemicals suitable forms antioxidant and cholesterol-reducing activities. Phytoestrogens are regarded as especially essential in the preventative measures of hormonally dependent malignancies such as breast cancer (BC). In this study lowered BC risk has been associated with whole grain/bran consumption with an odds ratio (OR=0.24 and 95 %CI=0.10-0.56). Wheat/bran appears to have a reliable protective impact against BC. While intake of white bread has been associated with a high risk of BC (OR=2.63 and CI 95 %=1.07-6.48). Also, the E-Selectin (SELE) Single nucleotide polymorphisms (SNPs) rs5353 A/G and rs932307 C/T were investigated using the sanger sequences approach. There was a positive association between genotypes (rs5353 GG+AG) and (rs932307 TT+CT) with rate consumption of wheat-white/bread and these genotypes were more frequent in patients had BC in comparison with a significant difference (P=0.03) (P=0.01), respectively. The genotypes (GG+AG) frequency of the rs5353 polymorphism and (TT+CT) genotypes of the rs932307 polymorphism in the present study had a high risk of cancer with (OR=3.05), (OR=4.17) respectively. While these genotypes showed no significant association with the rate of whole grain consumption in patients and control. Therefore, the type of wheat may associate with increased incidence of disease-related with type of Polymorphism because some present genotypes of SNPs showed high (OR) which may refer to their positive associated with disease, the white wheat consumption may active the risky association between SNPs and BC.

Application of conventional and modified cloud point extraction for simultaneous enrichment of cadmium, lead and copper in lake water and fish muscles

Muhammad Bilal,Tasneem G. Kazi,Hassan I. Afridi,Mohammad Balal Arain,Jameel A. Baig,Mustafa Khan,Naeemullah Khan 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.40 No.-

A new method based on modification of cloud point extraction for simultaneous enrichment ofcadmium, lead and copper in water and fish muscle samples was proposed. The extraction efficiency bymodified cloud point extraction (m-CPE) was compared with conventional cloud point extraction (c-CPE)method. The procedure for both CPE methods was comprised of formation of metal complexes with ahydrophobic chelating agent, dithizone, followed by entrapment of the chelates in a nonionic surfactant,Triton X-114. For c-CPE, the surfactant rich phase was treated with ethanolic solution of nitric acid andanalyzed by flame atomic absorption spectrometer (FAAS). Whereas for m-CPE, aqueous nitric acid wasused to back extract the metal ions from the surfactant rich phase and finally determined. The efficiencyof the methods was tested by analyzing certified reference material and standard addition to a realsample. All the experimental parameters were optimized. At optimized experimental conditions,preconcentration and enhancement factors were 62.5 and 78.0 to 83.0 respectively for m-CPE, 25.0 and56.0% higher than that of c-CPE. This improvement might be due to elimination of the effects ofsurfactant on the signal of analytes by FAAS. The developed method of m-CPE was applied successfullyfor analysis of the selected heavy metals in water and muscle tissues samples of fish of different lakes inSindh, Pakistan.

Associations between single-nucleotide polymorphisms of the interleukin-18 gene and breast cancer in Iraqi women

Zakariya, Bilal Fadil,Almohaidi, Asmaa M. Salih,Simsek, Secil Akilli,Kamal, Areege Mustafa,Al-Dabbagh, Wijdan H.,Al-Waysi, Safaa A. Korea Genome Organization 2022 Genomics & informatics Vol.20 No.2

According to long-term projections, by 2030, the world's population is predicted to reach 7.5 billion individuals, and there will be roughly 27 million new cancer cases diagnosed. The global burden of breast cancer (BC) is expected to rise. According to the Ministry of Health-Iraqi Cancer Registry, cancer is the second largest cause of death after cardiovascular disease. This study investigated the interleukin-18 (IL18) single-nucleotide polymorphisms (SNPs) -607C/A rs1946518 and -137G/C rs187238 using the sequence-specific amplification-polymerase chain reaction approach. Regarding the position -607C/A, there was a highly significant difference between the observed and expected frequencies in patients and controls (χ² = 3.16 and χ² = 16.5), respectively. The AA and CA genotypes were associated with significantly increased BC risk (odds ratio [OR], 3.68; p = 0.004 and OR, 2.83; p = 0.04, respectively). Women with the A allele had a 5.03-fold increased susceptibility to BC. The C allele may be a protective allele against BC (OR, 0.19). Although position -137G/C showed no significant differences in the CC genotype distribution (p = 0.18), the frequency of the CC genotype was significantly higher in patients than in controls. In contrast, patients had a significantly higher frequency of GC genotypes than controls (p = 0.04), which was associated with an increased risk of developing BC (OR, 2.63). The G allele frequency was significantly lower in patients than in controls (55.0% vs. 76.2%, respectively). This SNP may be considered a common genotype in the Iraqi population, with the wild-type G allele having a protective function (OR, 0.19) and the mutant C allele having an environmental effect (OR, 2.63).

Nosocomial oral myiasis by Sarcophaga sp. In Turkey

Suleyman Yazar,Bilal Dik,Saban Yalcin,Funda Demirtas,Ozan Yaman,Mustafa Ozturk,Izzet Sahin 연세대학교의과대학 2005 Yonsei medical journal Vol.46 No.3

We present a case of oral myiasis in a 15-year-old boy with tuberculosis meningitis. The diagnosis was based on the visual presence of wriggling larvae about 1 cm in size and on the microscopic features of the maggots, especially those relating to stigmatic structures. The larvae were identified as thirdstage larvae of Sarcophaga sp.

Chemical Compositional Characterization on Five Samples for Development of Artificial Bee Feed

김현지,이명렬,Bilal Mustafa,한기윤,이수진,권형욱 한국양봉학회 2020 韓國養蜂學會誌 Vol.35 No.4

The western honey bee, Apis mellifera L. is an essential pollinator for high yield in agriculture and provides products of economic values. Recently, the sudden decline of the bee population by numerous causes has occurred, which is called colony collapse disorder (CCD). One of the reasons in CCD, the limited nutrition in colonies declines brood rearing and shortens the lifespan of adult workers. Beekeepers need to provide artificial bee feed regularly to maintain health colony and continuity of bee-related products on apiculture. This study focuses on development of artificial bee feed, through investigation of different contents in nutritional components on five samples, namely canola pollen, mixed pollen, bee bread, MegaBee, and Test A. Among them, Test A was developed as artificial bee feed and was compared with other samples. The five samples were analyzed on free sugar, organic acid and amino acid. Sucrose content showed the largest amounts with 46.15% and 59.60% on Megabee and Test A, respectively. The Test A showed the largest account for citric acid with 92.33%. A total of 21 amino acids were detected on five samples. Among the detected 21 amino acids, proline was accounted the highest content in canola pollen, mixed pollen and bee bread with 1159.21 mg/L, 998.90 mg/L and 783.73 mg/L, respectively. Among the five samples, Test A showed the highest amount of lysine with 471.30 mg/L. Nutritional content, balance and efficiency needs to be considered for the development of artificial bee feed. This study will contribute to provide future directions on development of artificial bee feed.

The effect of the umami receptor (AmGr10) expression levels on growth and transcriptome in the hypopharyngeal glands of the western honey bees, Apis mellifera

Giyoun Han,Bilal Mustafa,Sujin Lee,Hyung Wook Kwon 한국응용곤충학회 2021 한국응용곤충학회 학술대회논문집 Vol.2021 No.04

Comparison of physicochemical properties of pollen substitute diet for honey bee (Apis mellifera)

Jee Kim Hyun,Hwang Jinseok,Ullah Zakir,Mustafa Bilal,Hyung Wook Kwon 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.4

Apis mellifera L., a globally important pollinator, is crucial in the maintenance of its colonies due to a variety of stresses. Healthy nutrition is believed to help grow their population and relieve the stressors. We used ourprepared and commercial diets, and the nutrition contents were then analyzed for free sugar, organic acids, free amino acids, inorganic ions, total polyphenols, vitamin C, and the pH. The total free sugar, organic contents, free amino acids, inorganic ions, and total polyphenols were in the range of 228.59 to 661.00, 0.91 to 20.60, 6.52 to 24.36, 10.25 to 27.61, and 1.65 to 10.17 mg/g, respectively. The highest contents of free sugar, organic acids, free amino acids, inorganic ions, and total polyphenols were found in YBNH (661 mg/g), Megabee (20.60 mg/g), Beebread (24.36 mg/g), AIGT + Soytide (27.61 mg/g), and Beebread (10.17 mg/g), respectively. The pH values were maintained at 4.45 to 5.70 among all ten samples. Group I showed the same level of total free sugars (Group II and Group IV), organic acids (Group II), inorganic ions (Group II, Group III, and Group IV), polyphenols (Group II), and pH (Group IV). For the total free amino acids, Group I showed a significantly higher (P < 0.05) level than that of Group II and Group III. Group I had a comparable nutrition content to the commercial bee diet but had a higher total free amino acid content. High levels of inorganic ions, free amino acids, and organic acids were found in the AIGT + Soytide.

Nutritional Compositional Characterization on Five Diets for Development of Pollen Substitute Diet

Hyunjee Kim,Myeong-lyeol Lee,Bilal Mustafa,Giyoun Han,Sujin Lee,Jinseok Hwang,Hyung Wook Kwon 한국양봉학회 2021 韓國養蜂學會誌 Vol.36 No.2

Application of modified cloud point extraction method for the chromium speciation in artificial saliva extracts of different snuff products

Asma Akhtar,Tasneem Gul Kazi,Hassan Imran Afridi,Mustafa Khan,Muhammad Bilal,Noman Khan 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.59 No.-

A modified cloud point extraction method (m-CPE) was developed for the speciation of chromium species (Cr3+ and Cr6+) in artificial saliva extracts (ASE) of snuff products. In this method, Cr3+ was complexed with 8-hydroxyquinoline, which was trapped in nonionic surfactant (Triton X-114), prior to analyzed by electrothermal atomic absorption spectrometer (ETAAS). Whereas, the total extractable Cr was determined by reducing Cr6+ to Cr3+ using Na2SO3 as a reducing reagent. Several parameters were optimized for the developed m-CPE. Under the most favorable conditions, enrichment factor, enhancement factor and limit of detection for the proposed method were 60, 134 and 0.04 μg L−1, respectively having relative standard deviation <5%. Health risks associated with the intake of total Cr in snuff products was also assessed. Estimated daily intake of Cr via sniffing 10 g/day of dry and moist snuff products was found below the maximum tolerable daily intake whereas the calculated risk for cancer due to Cr was observed in the acceptable range of 10 E−6–E−4.