Inhibition of oxidative stress induced‑cytotoxicity by coptisine in V79‑4 Chinese hamster lung fibroblasts through the induction of Nrf‑2 mediated HO‑1 expression

Hyeon‑Gyun Jo,Cheol Park,이혜숙,Gi‑Young Kim,Young‑Sam Keum,Jin Won Hyun,Taeg Kyu Kwon,Yung Hyun Choi,Su Hyun Hong 한국유전학회 2021 Genes & Genomics Vol.43 No.1

Background Coptisine is a natural alkaloid compound and is known to have multiple benefcial efects including antioxidant activity. However, whether it can protect lung fbroblasts from oxidative damage has not been studied yet. Objectives To investigate the potential inhibitory efect of coptisine against oxidative stress in V79-4 lung fbroblast cells. Methods V79-4 cells were treated with H2O2 (1 mM) in the presence or absence of coptisine (50 µg/ml), N-acetyl cysteine (NAC, 10 mM) or zinc protoporphyrin IX (ZnPP, 10 µM) for the indicated times. The alleviating efects of coptisine on cytotoxicity, cell cycle arrest, apoptosis, reactive oxygen species (ROS) production, DNA damage, mitochondrial dynamics, and inhibition of ATP production against H2O2 were investigated. Western blot analysis was used to analyze the expression levels of specifc proteins. Results Coptisine inhibited H2O2-induced cytotoxicity and DNA damage by blocking abnormal ROS generation. H2O2 treatment caused cell cycle arrest at the G2/M phase accompanied by increased expression of cyclin-dependent kinase (Cdk) inhibitor p21WAF1/CIP1 and decreased expression of cyclin B1 and cyclin A. However, these efects were attenuated in the presence of coptisine or NAC. Coptisine also prevented apoptosis by decreasing the rate of Bax/Bcl-2 expression in H2O2-stimulated cells and suppressing the loss of mitochondrial membrane potential and the cytosolic release of cytochrome c. In addition, the activation of nuclear factor-erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) was markedly promoted by coptisine in the presence of H2O2. However, zinc protoporphyrin IX, a potent inhibitor of HO-1, attenuated the ROS scavenging and anti-apoptotic efects of coptisine. Conclusions Based on current data, we suggest that coptisine can be used as a potential treatment for oxidative stress-related lung disease.

갑상선질환에서 혈청 Ferritin치의 의의

노흥규,한현석 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.2

In order to know the effect of thyroid hormone on the iron metabolism, serum ferritin levels obtained by the radioimmunoassay technique were analysed in 12 normal subjects(male : 5, female : 7), 15 hyperthyroid Graves' disease (male: 7, female: 8), 13 euthyroid Graves' disease (male : 4, female: 9) and 9 hypothyroid patients(female: 9) The results were as follows; 1. The serum ferritin levels of hyperthyroid patients were 96±88.2ng/ml in male and 68.6±41.2ng/ml in female and there was no significant difference compared with those of normal subjects. 2. The serum ferritin levels of euthyroid female patients and hypothyroid female patients were 27.5±14.9ng/ml and 23.5±17.5ng/ml respectively, with were significantly lower than that of hyperthyroid female patients (p<0.01) 3. There was significant correlation between serum T_4 and serum ferritin levels in all female patients(r=0.55, p<0.01).

助詞 "곳, 곰, 아"硏究

이현규 한국사회사업대학 1977 대학논문집 Vol.7 No.-

名詞形語尾「-(으)ㅁ, -기」의 史的 考察

李鉉奎 韓社大學 1975 대학논문집 Vol.5 No.-

In this paper, 1 studied historical changing of Ending 「-(?)m, -ki」from 15C to 18C. There are the same functions of grammar between {-(?)m} and {-ki}. Separating from the basic word, these morphemes have nonsense, but, combinating with a word, it has some meaning. Speaking exactly, they different in the sense. But we are used to thinking as the same sense each other. And then, taking historical survey, there are interesting phenomena in the usage of them, i. e they are able to replace each other. It means that they have the same function in grammar and meaning. In 15C, {-(?)m} is much superior to {-ki} as distribution in number. On the other hand, {-ki} is much superior to -(?)m in 18C. It is remarkable that distribution of two morphemes is similar to grammatical function, and it has changed differently according to a period. ① looking into the data of records, distribution of these Ending {-(?)m} and {-ki} has contrastly appeared. -(?)m is superior to {-ki} in 15C, while {-ki} is superior to {-(?)m} in 18C. ② In 15C, it has inserted prefinal Ending {-o/-u} front the -(?)m, it is to harmonize of vowel in phonology. It alterates, in several shaps, according to vowel to harmonize with the following vowel of the base word. Those alterations are {o}/o∼u∼jo∼ju∼a∼ø/. It is considered with phonological theory which had many alterations itself, and then, it has functions of insert, distinctive feature and stem-formative except function in harmony of vowel. ③ In 15C, the more distribution of the -(?) are caused by phonological theory according with way of writting, and by suitable forms in prose style. ④ Since 17C, Ending {-(?)m} has declined on the records. There are the reasons why vowel harmony declined, and 「-?/-?」 are confronted with 「-o/-u」according to losting 「-?」. It makes non-phonological usage that 「-o/-u」has disappeared. ⑤ Ending {-ki} was seldom distribution of records in 15C. It was superior to Ending -(?)m in 17C. There are some reasons why it is superior to {-(?)m} Ending {-ki} was more profitable that -(?)m in economical saving of labour and effectiveness of accoustics. On the other hand, it was that development of spoken language style was profitable to increase the usage of Ending {-ki}.

국산 폴리카보네이트 브라켓 윙의 파절 강도에 관한 연구

선민규,이기헌,황현식 대한치과교정학회 2007 대한치과교정학회지 Vol.37 No.3

秦始皇陵 출토 병용의 服飾 硏究 : 胡服과의 관련성을 중심으로

김소현,조규화 한국의류학회 1993 한국의류학회지 Vol.17 No.1

This study intends to find out the correspondence of the costumes of the terracotta warriors at the tomb of Qin Shi Huang to the dress of nomadic people. The Chinese took the dress of nomadic people into their costumes so that they might practise shooting arrows from horse back in the Age Of Wars. The terracotta warriors at the tomb of Qin Sin Huang provide us with substantial evidence that Qin Dynasty inherited the system of wearing the dress of nomadic people from Zhao. Figures of calvarys wear the jackets of nomadic people, but figures of other warriors wear Han jackets. This means that the aim of taking the dress of nomadic people is to be convenient in practising shooting arrows from horse back. Nomadic people adjusted their jackets to left, because they road on a horse from left for being the tunga only on the left. But calvarys adjusted their jackets to right. In those days, China didn't have a tunga. So there was no necessity for riding from left. And Chinese were accustomed to adjusted their jackets to right. This is the reason why calvarys adjusted their jackets to right, though they wear the dress of nomadic people. Soldiers wear trousers. Some have puttee, some have their leg bounds. All the soldiers tightened their coats or jackets with belts which were inhereted from nomadic people. They wear either shoes or short boots. The figures are classified according to rank, espicially in their hats and armours. General wears a cap adorned with pheasant' feathers, officer wears an unadorned cap, and men are hatless or in turbans. Calvary's headgear looks like a p'ing-chin-tse which was correspondence to the headgear of nomadic people. Soldiers wear armours according to duty, and armours are differentiated by rank. From the state of terracotta warriors, I think that the warriors were made from BC 246 to BC 210 which is equal to the date of making the tomb of Qin Shi Huang. Then the date of making the terracotta warriors was between 60 and 100 years since the king Wu Ling of the state of Zhao took the dress of nomadic people.

3차원 콘빔CT 영상에서 형성한 2차원 정모두부방사선사진영상의 정확도 증진을 위한 두부자세재현기의 효과

선민규,엄기수,조진형,황현식 대한치과교정학회 2009 대한치과교정학회지 Vol.39 No.5

Objective: The purpose of the present study was to evaluate the effectiveness of the use of Head Posture Aligner (HPA) during cone-beam computed tomography (CBCT) scan in generation of frontal cephalograms using 3D CBCT images. Methods: CBCT scans and frontal cephalograms were made in 30 adult individuals. While a couple of CBCT scan was made for one subject, one was made with conventional method, without use of HPA, the other was acquired with the use of HPA. After creation of virtual frontal cephalogram from each 3D CBCT image, it was traced and compared with the tracing of real frontal cephalogram. Results: In the comparison of the measurements, the virtual cephalograms with the use of HPA did not show statistically significant differences with the real cephalograms whereas the virtual cephalograms without the use of HPA presented significant differences with real cephalograms in many measurements. In the correlation analysis with the measurements of the real cephalograms, the virtual cephalograms with the use of HPA showed higher correlations in all measurements than the virtual cephalograms without the use of HPA. Conclusions: Measurements from CBCT-generated cephalograms become similar to those from real cephalograms with the use of HPA during CBCT scan. Thus, the use of HPA is suggested during the CBCT scan in order to construct accurate virtual frontal cephalograms using 3D CBCT images. 본 연구는 3차원 콘빔CT 영상을 이용하여 2차원 정모두부방사선사진영상 형성 시 두부자세재현기(Head Posture Aligner, HPA)를 이용한 경우와 이용하지 않은 경우를 비교분석함으로써 HPA의 유용성을 평가하기 위하여 시행되었다. 성인 30명을 대상으로 먼저 일반적인 방법으로 콘빔CT 영상을 채득한 후 HPA를 이용하여 일정한 두부자세를 재현한 상태에서 또 하나의 콘빔CT 영상 및 정모두부방사선사진을 촬영하였다. 3차원 영상프로그램을 이용하여 HPA를 이용하지 않은 경우와 HPA를 이용한 경우 각각에서 2차원 정모두부방사선사진영상을 형성한 다음 각각의 투사도를 작성하고 실제로 HPA를 이용하여 촬영된 정모두부방사선사진 투사도와의 차이를 비교하였다. 거리 및 각도를 나타내는 여러 계측치를 비교분석한 결과 HPA를 이용하지 않은 경우에는 다수의 계측항목에서 실제 정모두부방사선사진과 유의한 차이를 보인 반면 HPA를 이용한 경우에는 모든 항목에서 통계적 유의차를 보이지 않았다. 아울러 실제 정모두부방사선사진과의 상관관계를 알아보기 위해 상관분석을 시행한 결과 HPA를 이용한 경우가 그렇지 않은 경우보다 모든 항목에서 높은 상관성을 보였다. 본 연구 결과는 3차원 콘빔CT 영상을 이용하여 2차원 정모두부방사선사진영상 형성 시 정확한 영상 획득을 위해서는 콘빔CT 촬영 시 HPA의 사용이 필요함을 시사하였다.

설측브라켓 부착시 위치오차에 관한 연구

최준규,황현식,김종철 대한치과교정학회 1998 대한치과교정학회지 Vol.28 No.1

본 연구는 설측브라켓 부착시 부착방법에 따른 위치오차를 알아보기 위하여 시행되었다. 교정환자 20명의 초진시 석고모형을 연구대상으로 하여, 초진모형의 각 치면에 긴밀하게 브라켓을 부착하는 방법, 치아재배열을 시행한 후 치면에 긴밀하게 부착하는 방법 그리고 치아재배열을 시행하고 이상호선을 제작한 후 브라켓을 결찰하여 부착하는 passive bracketing의 3가지 방법으로 브라켓을 부착한 후 각 방법으로 부착된 브라켓의 위치오차를 측정 및 비교하여 다음과 같은 결과를 얻었다. 1. 각도오차 분석결과, 치아재배열을 시행하지 않은 경우 상악 제1소구치와 하악견치의 위치오차가 타 방법에 비하여 크게 나타났으며, passive bracketing을 시행하지 않은 경우 상악 중절치, 하악 제1·제2소구치에서 그 위치오차가 크게 나타났다. 2. 토크오차 분석결과, 치아재배열을 시행하지 않은 경우 하악 제2소구치의 위치오차가 타 방법에 비해 크게 나타났으며 passive bracketing을 시행하지 않은 경우 상악 전 치아와 하악 제1·제2소구치에서 그 위치오차가 크게 나타났다. 3. 회전오차 분석결과, passive bracketing을 시행하지 않은 경우 상악 중절치간, 하악 중절치간, 하악 측절치와 중절치사이 그리고 하악 견치와 측절치 사이에서 그 위치오차가 크게 나타났다. 4. 내외오차 분석결과, passive bracketing을 시행하지 않은 경우 상악 견치와 측절치 사이에서 위치오차가 타 방법에 비해 크게 나타났으며, passive bracketing을 시행하지 않은 경우 상악 중절치간, 측절치와 중절치 사이, 제1·제2소구치사이, 하악 측절치와 중절치 사이, 견치와 측절치 사이에서 그 위치오차가 크게 나타났다. 본 연구 결과는 설측교정술의 경우 브라켓 간접부착 술식을 사용하더라도 상당량의 위치오차가 나타나며, 이는 passive bracketing으로 줄일 수 있음을 시사하였다. The purpose of this study was to evaluate the positioning errors according to the method of bonding lingual brackets. Dental models of twenty orthodontic patients with malocclusion were selected for this study. The positioning errors were measured on each model that brackets were bonded to. Three different bonding methods were used. For the first method, the bracket was bonded intimately to the lingual surface of the model. For the second method, the bracket was bonded intimately to the lingual surface after setting up using articulator. The passive bracketing, bonding the bracket ligated first to ideal archwire, was used after setting up as the last method. The results were as follows: 1. The brackets bonded without setting up showed greater angulation errors in the upper 1st premolar and the lower canine than those in other bonding methods. The brackets bonded without passive bracketing showed greater positioning errors in upper central incisor, lower 1st and 2nd premolars. 2. The brackets bonded without setting up showed greater torque error in lower 2nd premolar than those in other bonding methods. The brackets bonded without passive bracketing showed greater torque errors in all upper teeth, lower 1st and 2nd premolars. 3. The brackets bonded without passive bracketing showed greater rotation errors between upper central incisors, lower central incisors, lower lateral and central incisor, lower canine and lateral incisor. 4. The brackets bonded without setting up showed greater in-out errors between upper canine and lateral incisor than those in other bonding methods. The brackets bonded without passive bracketing showed greater in-out errors between upper central incisors upper central and lateral incisors, upper 1st and 2nd premolars, lower lateral and central incisors, lower canine and lateral incisor. These results suggest that there is a large amount of positioning error in lingual brackets even by an indirect bonding technique, and it may be reduced by passive bracketing.

言語와 論理 (Ⅰ)

曺圭太,韓載賢,李基用 全北大學校 語學硏究所 1977 어학 Vol.4 No.-

흰쥐 경동맥 손상 모형에서 체외 방사선조사에 의한 신내막 과증식억제 : Intercellular Adhesion Molecule-1과 Vascular cell Adhesion Molecule-1의 역할 The Possible Role of Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1

강동헌,승기배,장기육,임상현,정해억,임효영,김연실,채장성,김재형,홍순조,최규보 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.9