Rocaglamide-A Potentiates Osteoblast Differentiation by Inhibiting NF-κB Signaling

Aiguo Li,Libin Yang,Xiaolin Geng,Xingmei Peng,Tan Lu,Yanjun Deng,Yuzheng Dong 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.11

Rheumatoid arthritis is a chronic inflammatory disease that leads to bone and cartilage erosion. The inhibition of osteoblast differentiation by the inflammatory factor TNF-α is critical for the pathogenesis of rheumatoid arthritis. To modulate TNF-α mediated inhibition of osteoblast differentiation is required to improve therapeutic efficacy of rheumatoid arthritis. Here, we explored the potential role of rocaglamide-A, a component of Aglaia plant, in osteoblast differentiation. Rocaglamide-A prevented TNF-α mediated inhibition of osteoblast differentiation,and promoted osteoblast differentiation directly,in both C2C12 and primary mesenchymal stromal cells. Mechanistically, Rocaglamide- A inhibited the phosphorylation of NF-κB component p65 protein and the accumulation of p65 in nucleus, which resulted in the diminished NF-κB responsible transcriptional activity. Oppositely, overexpression of p65 reversed rocaglamide-A’s protective effects on osteoblast differentiation. Collectively, rocaglamide-A protected and stimulated osteoblast differentiation via blocking NF-κB pathway. It suggests that rocaglamide-A may be a good candidate to develop as therapeutic drug for rheumatoid arthritis associated bone loss diseases.

Effect of Channel Interaction and Presentation Level on Speech Recognitionin Simulated Bilateral Cochlear Implants

Yang-soo Yoon,Aiguo Liu,You-Ree Shin,Jae-sook Gho,Candace Hicks,Qian-Jie Fu,Allison Coltisor 한국언어재활사협회 2016 Clinical Archives of Communication Disorders Vol.1 No.1

Purpose: The present study used the acoustic simulation of bilateral cochlear implants to investigate the effect of presentation level (EXP 1) and channel interaction (EXP 2) on binaural summation benefit in speech recognition. Methods: The acoustic 6-channel processors were constructed, and the envelope of each band was used to modulate a sinusoid (EXP 1) or band-pass filtered white noise (EXP 2) with a frequency matching the center frequency of the carrier band. Presentation level was fixed for one ear and varied for the other. Channel interaction was simulated by altering filter slopes while keeping interaural spectral peaks matched in the carrier bands. Two separate groups of ten adult subjects with normal hearing participated in EXP 1 and EXP 2. Sentence recognition was measured with left ear alone, right ear alone, and both ears in quiet and in noise at +5 dB signal-to-noise ratio. Results: A significant binaural summation benefit occurs only in noise, regardless of interaural mismatch in channel interactions and presentation levels. Conclusions: Results suggest that factors other than channel interaction are important and that matching interaural loudness is an unimportant factor in binaural summation benefit in noise. For both EXP 1 and EXP 2, the data trend is indicative of speech information perceived in quiet being fully coded by the better performing ear alone, which leads to no binaural summation benefit. The results of current and future studies could have implications in programming of bilateral cochlear implant users.

Polygonumnolides A1–B3, minor dianthrone derivatives from the roots of Polygonum multiflorum Thunb

Jianbo Yang,Zheng Yan,Jin Ren,Zhong Dai,Shuangcheng Ma,Aiguo Wang,Yalun Su 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.6

Seven new dianthrone glycosides, named polygonumnolidesA1–B3 (1–7), were isolated from the 70 %EtOH extract of the dried roots of Polygonum multiflorumThunb. using column chromatography and preparativehigh-performance liquid chromatography. Their structureswere determined by 1D and 2D NMR and mass spectroscopy. The isolated compounds were evaluated for theircytotoxic effects against KB tumor cell lines and compounds1–4 showed moderate cytotoxicity.

Challenges in neuro-machine interaction based active robotic rehabilitation of stroke patients

Song, Aiguo,Yang, Renhuan,Xu, Baoguo,Pan, Lizheng,Li, Huijun Techno-Press 2014 Advances in robotics research Vol.1 No.2

Study results in the last decades show that amount and quality of physical exercises, then the active participation, and now the cognitive involvement of patient in rehabilitation training are known of crux to enhance recovery outcome of motor dysfunction patients after stroke. Rehabilitation robots mainly have been developing along this direction to satisfy requirements of recovery therapy, or focusing on one or more of the above three points. Therefore, neuro-machine interaction based active rehabilitation robot has been proposed for assisting paralyzed limb performing designed tasks, which utilizes motor related EEG, UCSDI (Ultrasound Current Source Density Imaging), EMG for rehabilitation robot control and feeds back the multi-sensory interaction information such as visual, auditory, force, haptic sensation to the patient simultaneously. This neuro-controlled and perceptual rehabilitation robot will bring great benefits to post-stroke patients. In order to develop such kind of robot, some key technologies such as noninvasive precise detection of neural signal and realistic sensation feedback need to be solved. There are still some grand challenges in solving the fundamental questions to develop and optimize such kind of neuro-machine interaction based active rehabilitation robot.

Rocaglamide-A Potentiates Osteoblast Differentiation by Inhibiting NF-κB Signaling

Li, Aiguo,Yang, Libin,Geng, Xiaolin,Peng, Xingmei,Lu, Tan,Deng, Yanjun,Dong, Yuzheng Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.11

Rheumatoid arthritis is a chronic inflammatory disease that leads to bone and cartilage erosion. The inhibition of osteoblast differentiation by the inflammatory factor TNF-${\alpha}$ is critical for the pathogenesis of rheumatoid arthritis. To modulate TNF-${\alpha}$ mediated inhibition of osteoblast differentiation is required to improve therapeutic efficacy of rheumatoid arthritis. Here, we explored the potential role of rocaglamide-A, a component of Aglaia plant, in osteoblast differentiation. Rocaglamide-A prevented TNF-${\alpha}$ mediated inhibition of osteoblast differentiation, and promoted osteoblast differentiation directly, in both C2C12 and primary mesenchymal stromal cells. Mechanistically, Rocaglamide-A inhibited the phosphorylation of NF-${\kappa}B$ component p65 protein and the accumulation of p65 in nucleus, which resulted in the diminished NF-${\kappa}B$ responsible transcriptional activity. Oppositely, overexpression of p65 reversed rocaglamide-A's protective effects on osteoblast differentiation. Collectively, rocaglamide-A protected and stimulated osteoblast differentiation via blocking NF-${\kappa}B$ pathway. It suggests that rocaglamide-A may be a good candidate to develop as therapeutic drug for rheumatoid arthritis associated bone loss diseases.

Hopf Bifurcation Study of Inductively Coupled Power Transfer Systems Based on SS-type Compensation

Xia, Chenyang,Yang, Ying,Peng, Yuxiang,Hu, Aiguo Patrick The Korean Institute of Power Electronics 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.3

In order to analyze the nonlinear phenomena of the bifurcation and chaos caused by the switching of nonlinear switching devices in inductively coupled power transfer (ICPT) systems, a Jacobian matrix model, based on discrete mapping numerical modeling, is established to judge the system stability of the periodic closed orbit and to study the nonlinear behavior of Hopf bifurcation in a system under full resonance. The general flow of the parameter design, based on the stability principle for ICPT systems, is proposed to avoid the chaos and bifurcation phenomena caused by unreasonable parameter selection. Firstly, based on the state equation of SS-type compensation, a three-dimensional bifurcation diagram with the coupling coefficient as the bifurcation parameter is established with a numerical simulation to observe the nonlinear phenomena in the system. Then Filippov's method based on a Jacobian matrix model is adopted to deduce the boundary of stable operation and to judge the type of the bifurcation in the system. Then the general flow of the parameter design based on the stability principle for ICPT systems is proposed through the above analysis to realize stable operation under the conditions of weak coupling. Finally, an experimental platform is built to confirm the correctness of the numerical simulation and modeling.

Hopf Bifurcation Study of Inductively Coupled Power Transfer Systems Based on SS-type Compensation

Chenyang Xia,Ying Yang,Yuxiang Peng,Aiguo Patrick Hu 전력전자학회 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.3

In order to analyze the nonlinear phenomena of the bifurcation and chaos caused by the switching of nonlinear switching devices in inductively coupled power transfer (ICPT) systems, a Jacobian matrix model, based on discrete mapping numerical modeling, is established to judge the system stability of the periodic closed orbit and to study the nonlinear behavior of Hopf bifurcation in a system under full resonance. The general flow of the parameter design, based on the stability principle for ICPT systems, is proposed to avoid the chaos and bifurcation phenomena caused by unreasonable parameter selection. Firstly, based on the state equation of SS-type compensation, a three-dimensional bifurcation diagram with the coupling coefficient as the bifurcation parameter is established with a numerical simulation to observe the nonlinear phenomena in the system. Then Filippov’s method based on a Jacobian matrix model is adopted to deduce the boundary of stable operation and to judge the type of the bifurcation in the system. Then the general flow of the parameter design based on the stability principle for ICPT systems is proposed through the above analysis to realize stable operation under the conditions of weak coupling. Finally, an experimental platform is built to confirm the correctness of the numerical simulation and modeling.

The Mathematic Model of "Pressing Complexion" Differential Coefficient Countermeasure Decision for Collision-avoidance

Cai Feng,Shi Aiguo,Yang Baozhang,Zhou Lixin 한국항해항만학회 2001 한국항해항만학회 학술대회논문집 Vol.3 No.-

In this article, we have done some analysis about the collision-avoidance specialty of "pressing complexion" and "pressing danger" in the meet process of two boats, and offered a mathematic model of differential coefficient countermeasure decision for collision-avoidance. which adapt to the right complexion. The basal idea is, in the right condition whatever do the coming boat do, and our boat will always adopt dynamic, continuous and the best countermeasure. When both the controlling capabilities of two boats have advantage and inferior position, we can working-out with the qualitative differential coefficient countermeasure.

Study on the mixing performance of mixing vane grids and mixing coefficient by CFD and subchannel analysis code in a 5×5 rod bundle

Han Bin,Zhu Xiaoliang,Yang Bao-Wen,Liu Aiguo,Xi Yanyan,Liu Lei,Liu Shenghui,Huang Junlin 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.10

Mixing Vane Grid (MVG) is one of the most important structures in fuel assembly due to its high performance in mixing the coolant and ultimately increasing Critical Heat Flux (CHF), which avoids the temperature rising suddenly of fuel rods. To evaluate the mixing performance of the MVG, a Total Diffusion Coefficient (TDC) mixing coefficient is defined in the subchannel analysis code. Conventionally, the TDC of the spacer grid is obtained from the combination of experiments and subchannel analysis. However, the processing of obtaining and determine a reasonable TDC is much challenging, it is affected by boundary conditions and MVG geometries. In is difficult to perform all the large and costing rod bundle tests. In this paper, the CFD method was applied in TDC analysis. A typical 5 5 MVG was simulated and validated to estimate the mixing performance of the MVG. The subchannel code was used to calculate the TDC. Firstly, the CFD method was validated from the aspect of pressure drop and lateral temperature distribution in the subchannels. Then the effect of boundary conditions including the inlet temperature, inlet velocities, heat flux ratio between hot and cold rods and the arrangement of hot and cold rods on MVG mixing and TDC were studied. The geometric effects on mixing are also carried out in this paper. The effect of vane pattern on mixing was investigated to determine which one is the best to represent the grid's mixing performance.

Development of EST-SSRs from the ark shell (Scapharca broughtonii) transcriptome and their application in genetic analysis of four populations

Wenjing Wang,Biao Wu,Zhihong Liu,Liqing Zhou,Xiujun Sun,Jiteng Tian,Aiguo Yang 한국유전학회 2021 Genes & Genomics Vol.43 No.6

Background The ark shell (Scapharca broughtonii) is one of the most economically important mollusks in the Bohai Sea and Yellow Sea of China. In recent years, ark shells from the Korean population were introduced to China for seed propagation and culture. Objective To explore the impact of the introduction of Korean ark shell on the genetic diversity of native population in China. Methods Simple sequence repeat (SSR) is efective and widely used tool for genetic analysis. In this study, 180 EST-SSRs were selected and verifed by polymerase chain reaction and polyacrylamide gel electrophoresis. Subsequently, fve polymorphic EST-SSRs were screened and their primers were modifed by fuorescein for use in the genetic analysis of four populations. Results Genetic analysis showed that 361 alleles amplifed by fve SSR loci were detected in the four populations. The number of alleles for the fve SSRs ranged from 8 to 30, with a mean of 18.05 (standard deviation, SD=6.492). The efective number of alleles varied from 2.253 to 22.222, with a mean of 10.596 (SD=4.713). Observed heterozygosity and expected heterozygosity were 0.167–0.833 and 0.566–0.971, with average values of 0.520 (SD=0.177) and 0.891 (SD =0.062), respectively. Polymorphic information content ranged from 0.521 to 0.953, with a mean of 0.865 (SD=0.070). The pairwise genetic diferentiation coefcient (FST) of the four populations ranged from 0.0267 to 0.0477, showing low genetic diferentiation. The phylogenetic tree constructed by neighbor-joining method showed that the genetic distance between the Chinese Dalian native population and three Korean populations was relatively more far than that among those Korean populations. Conclusion The results indicated that the genetic structure of the Dalian wild population was less afected by the introduced Korean wild populations.