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L-ascorbic acid가 포집된 BGsome의 특성 및 안정화 효과
황수연 ( Sue Yun Hwang ),진병석 ( Byung Suk Jin ) 한국유화학회 2011 한국응용과학기술학회지 Vol.28 No.3
Encapsulation of L-ascorbic acid(AA) into BGsome was attempted to improve its stability. BGsome is a bio-compatible vesicular system prepared by dispersion of hydrated liquid crystalline phase formed through hydration of 1,3-butylene glycol(BG)-dissolved lecithin with an aqueous solution containing hydrophilic component. The characteristics of AA encapsulated BGsome, such as droplet size, surface charge, and solution appearance, was investigated. The concentration of AA solution had considerable effect on droplet size and surface charge of BGsome. Several tens nanometer droplet made by sonication treatment did not showed any change of size with storage time. Stability of AA was improved by encapsulation into BGsome, which was verified through DPPH test and HPLC assay.
콜라겐으로 경구 관용을 유도한 관절염 동물 모델의 세포 특이적 면역 반응 조사
민소연,황수연,이재선,김주영,이강은,김경운,김영훈,도주호,김호연,Min, So-Youn,Hwang, Sue-Yun,Lee, Jae-sun,Kim, Ju-Young,Lee, Kang-Eun,Kim, Kyung-Wun,Kim, Young-Hun,Do, Ju-Ho,Kim, Ho-Youn 대한면역학회 2003 Immune Network Vol.3 No.2
Oral administration of antigen has long been considered as a promising alternative for the treatment of chronic autoimmune diseases including rheumatoid arthritis (RA), and oral application of type II collagen (CII) has been proven to improve pathogenic symptoms in RA patients without problematic side effects. To further current understandings about the immune suppression mechanisms mediated by orally administered antigens, we examined the changes in IgG subtypes, T-cell proliferative response, and proportion of interleukin (IL)-10 producing Th subsets in a time course study of collagen induced arthritis (CIA) animal models. We found that joint inflammation in CIA mouse peaked at 5 weeks after first immunization with CII, which was significantly subdued in mice pre-treated by repeated oral administration of CII. Orally tolerized mice also showed increase in their serum level of IgG1, while the level of IgG2a was decreased. T-cell proliferation upon CII stimulation was also suppressed in lymph nodes of mice given oral administration of CII compared to non-tolerized controls. When cultured in vitro in the presence of CII, T-cells isolated from orally tolerized mice presented higher proportion of $CD4^+IL-10^+$ subsets compared to non-tolerized controls. Interestingly, such increase in IL-10 producing cells were obvious first in Peyer's patch, then by 5 weeks after immunization, in mesenteric lymph node and spleen instead. This result indicates that a particular subset of T-cells with immune suppressive functions might have migrated from the original contact site with CII to inflamed joints via peripheral blood after 5 weeks post immunization.
류마티스 관절염 환자에서 Conserved T 세포 수용체의 CDR3 motif를 표현하는 제2형 콜라겐 특이 T세포주의 형성과 유지
김승훈,조미라,윤지희,박성환,조철수,황수연,김호연,Kim, Seung-Hoon,Cho, Mi-La,Youn, Jeehee,Park, Sung-Hwan,Hwang, Sue-Yun,Cho, Chul-Soo,Kim, Ho-Youn 대한면역학회 2001 Immune Network Vol.1 No.1
Background: To determine the molecular structure of type II collagen-specific T-cell receptors associated with rheumatoid arthritis (RA). Methods: We generated CII-specific T-cell lines of 8 RA patients by prolonged in vitro culture with bovine CII (bCII) and the immunogenic peptide (256-270) of human CII. The proliferation response towards CII stimulation was measured from the uptake of 3H-thymidine. Changes in the secretion of Th 1 and Th2 cytokines in the culture supernatent were measured by ELISA. The TCR clonotypes of these T-cells were examined by RT-PCR/SSCP analyses of all 22 $V_{\beta}$ chains. Results: T-cells from patients' tissue exhibited strong proliferation index upon CII stimulation, which was maintained up to 6 months in the culture. The secretion of INF-$\gamma$from these T-cells increased along with the duration of culture time, while the amount of IL-4 production did not show significant changes. The SSCP band patterns of patients' T-cells appear as discrete bands unlike the smeary streak produced from normal samples. Some SSCP bands, each representing selected expansion of a TCR containing certain subtype of $V_{\beta}$ peptides, appeared to be identical in more than one patients. Among these, the expansion of SSCP band representing the $V_{\beta}$ 14 CDR3 region persisted after switching the antigen to the immunogenic human peptide (256-270). Conclusion: CII-reactive T-cells expressing distinct CDR3 motifs are selectively expanded in the peripheral blood and synovial fluid of RA patients, and their persistent proliferation upon CII stimulation, as well as the production Th 1-type cytokines, may play pivotal roles in RA pathogenesis.
Amoeba proteus 의 공생주와 비공생주간의 동위효소 양상의 비교
안태인,황수연 한국유전학회 1986 Genes & Genomics Vol.8 No.2
Cytosol proteins of tD and xD strain of A. proteus were electrophoresed in polyacrylamide gel, and the patterns of the 11 enzymes were compared between the mother strain and the variant strain established by bacterial infection. Of the 14 enzymes tested in this study, 5 oxidoreductases, 3 hydrolases, 2 transferases, and 1 lysase were detected in Amoeba. Among these, malic enzyme, isocitrate dehydrogenase, hexokinase and adenylate kinase were monomorphic, while alcohol dehydrogenase, lactate dehydrogenase, esterase and aldolase were dimorphic enzymes. Malate dehydrogenase, alkaline phosphatase and acid phosphatase were polymorphic enzymes. Apparent strain specificity was shown in xD strain as an additional isozyme of malate dehydrogenase.
Diversification of the Biochemical Strain Specificity in a Symbiotic Strain of Amoeba proteus
안태인,최의열,황수연 한국유전학회 1986 Genes & Genomics Vol.8 No.1
Diversification of the biochemical strain specificity between tD and symbiotic xD strain of A. proteus was studied by the quantitative analysis of purine and nitrogen catabolites, and also by the analysis of 10 different isozymes. Among the purine catabolites, the amount of carbonyl diurea-crystals in xD strain was reduced to 60% compared with that in tD strain. The level of ammonia and biuret reactant in xD cytoplasm decreased to 46% and 21 % of those in tD cytoplasm, respectively. In the analysis of isozymes 4 monomorphic, 3 dimorphic and 3 polymorphic enzymes were detected in amoebae. Among them the patterns of polymorphic malate dehydrogenase and alkaline phosphatase of xD strain were different from tD strain. Those different isozyme bands showed partial coincidence with the isozyme bands of bacterial endosymbiont. From these results above, the symbiotic bacteria in xD strain appears to utilize the metabolic wastes of nitrogen and purine catabolism of the host. The levels of cytoplasmic free ammonia, biuret, and carbonyl diurea and the isozyme patterns of alkaline phosphatase and malate dehydrogenase could be the possible genetic markers for diversification of biochemical strain specificity of the two strains that had been diverged recently.