황해산 대하(Penaeus chinensis)의 계군분석을 위한 미토콘드리아 DNA 분석

황규린,이영철,장정순,HWANG Gyu-Lin,LEE Yong-Chul,CHANG Chung-Soon 한국수산과학회 1997 한국수산과학회지 Vol.30 No.1

황해 서식 대하(P. chinenesis) 각 개체군의 유전 구조를 파악하기 위하여 미토콘드리아 DNA(mtDNA)를 추출한 후 BamHI등 15종의 제한효소를 이용한 제한효소 절편 다형 현상 (Restriction Fragment Length Polymer-phisms; RFLPs)을 분석하였으며 보리새우 (P. japonicus)와의 염기 치환 정도를 비교하기 위하여 일본 나가사키 1 집단의 절편 양상도 함께 분석하였다. 한국의 대천, 진도, 나로도 집단과 중국의 발해, 청도 집단 등 5개 집단을 분석한 결과, 대하에서는 3종의 haplotype이 발견되었으나 5개 집단 모두가 하나의 haplotype에 의해 지배되고 있는 것으로 나타났다. 나타난 변이는 2종류로 Cla I과 Pvu II 인식부위가 부가된 변이가 청도와 나로도 집단에서 출현하였을 뿐 전 집단에 대한 나머지 13종의 제한 효소 절편 양상은 모두 동일한 것으로 나타났다. 이와 같은 결과에서 보면 황해산 대하의 각 집단 사이에는 활발한 유전자 교류가 있는 것으로 판단되며, 3개 계군에 대한 지금까지의 구분은 재검토되어야 할 것으로 판단된다. 대하와 보리새우의 mtDNA 공통절편을 이용한 p 값에 의한 염기치환은 $13.7\%$ 정도인 것으로 나타났으며, 제한 효소별 절편의 크기를 비교한 결과 대하의 mtDNA의 크기는 평균 16.44kb였고 보리새우는 약 16.31kb였다. The mitochondrial DNA (mtDNA) restriction fragment length polymorphi는 (RFLPs) of five populations were analyzed to delineate the stocks of Penaeus chinensis (Osbeck) in the Yellow Sea. Comparison of P. chinensis with P. japonicus to clarify the nucleotide divergence between two species was also carried out. Based on the fragment patterns, three composite haplotypes were analyzed in P. chinensis mtDNA as four naplotypes were in P. japonicus. Most individuals of each P. chinensis population are shared by one dominant haplotype. Another two haplotypes haying variations at the C/a I and hull sites were also distributed evenly in the Korean and Chinese populations. It is suggested that the gene exchange occurring between populations in the Yellow Sea is frequent. Average length of the mtDNA molecule was estimated to be about 16.44 kb in P. chinensis and 16.31 kb in P. japonicus, Sequence divergence (p) of mtDNA between two species estimated by using Upholt's (1977) fomula was $13.7\%$.

Feasibility of Revision Cochlear Implant Surgery for Better Speech Comprehension

황규린,이재용,오현석,이병돈,정진세,최재영 대한청각학회 2019 Journal of Audiology & Otology Vol.23 No.2

Background and Objectives: The purpose of this study was to evaluate the efficacy of revision cochlear implant (CI) surgery for better speech comprehension targeting patients with low satisfaction after first CI surgery. Subjects and Methods: Eight patients who could not upgrade speech processors because of an too early CI model and who wanted to change the whole system were included. After revision CI surgery, we compared speech comprehension before and after revision CI surgery. Categoies of Auditory Performance (CAP) score, vowel and consonant confusion test, Ling 6 sounds, word and sentence identification test were done. Results: The interval between surgeries ranged from eight years to 19 years. Same manufacturer’s latest product was used for revision surgery in six cases of eight cases. Full insertion of electrode was possible in most of cases (seven of eight). CAP score (p-value= 0.01), vowel confusion test (p-value=0.041), one syllable word identification test (p-value= 0.026), two syllable identification test (p-value=0.028), sentence identification test (pvalue= 0.028) had significant improvement. Consonant confusion test (p-value=0.063), Ling 6 sound test (p-value=0.066) had improvement but it is not significant. Conclusions: Although there are some limitations of our study design, we could identify the effect of revision (upgrade) CI surgery indirectly. So we concluded that if patient complain low functional gain or low satisfaction after first CI surgery, revision (device upgrade) CI surgery is meaningful even if there is no device failure

A Cervical Vagal Schwannoma Mimicking a Parathyroid Cyst

황규린,김재욱,김희경,이승원 대한이비인후과학회 2014 Clinical and Experimental Otorhinolaryngology Vol.7 No.2

Cervical vagal schwannomas with cystic degeneration changes are extremely rare. These tumors are typically benign and slow growing. A 44-year-old woman underwent complete resection of a tumor measuring 4×3.5 cm in the right neck using the endoscopic approach, instead of the conventional transcervical approach. We applied the new scarless neck surgery technique with an endoscopic unilateral axillo-breast approach. The tumor originated from the right vagus nerve, and was confirmed to be a vagal schwannoma pathologically. The patient has been followed up for 18 months postoperatively with no evidence of tumor recurrence or neurological deficit. We report an extremely rare vagal schwannoma with cystic degen- eration that was removed by an endoscopic approach, along with a review of the relevant literature.

황해산 참조기 (Pseudosciaena polyactis Bleeker)의 mitochondrial DNA 분석

황규린,이영철,장정순,허회권,HWANG Gyu-Lin,LEE Yong-Chul,CHANG Chung-Soon,HUE Hoi-Kwon 한국수산과학회 1994 한국수산과학회지 Vol.27 No.5

황해에 서식하는 참조기(Pseudosciaena polyactis Bleeker) 각 계군의 유전적 차이점을 분석하기 위하여 중국에서 3지역(Zhoushan, Shanghai, Qingdao), 한국 2지역(목포, 인천)에서 채집된 참조기로부터 mitochondrial DNA(mtDNA)의 RFLP(제한효소 절편 다형현상)를 분석하였다. 총 18종의 제한효소를 이용하여 처리한 결과 5개 집단 모두 동일한 크기인 $16.9{\pm}0.6kb$의 mtDNA를 소유한 것으로 나타났으며 이는 다른 어류군들과 유사한 크기였다. 참조기 mtDNA에 대한 RFLP 분석을 행한 결과 각 집단 마다 대략 40여개의 절편이 관찰되었고 5개 집단 모두 동일한 mtDNA 절편양상을 보였으나 사용된 제한효소 중 좌ApaI, EcoRI, PstI, SstII 및 SmalI에서 중국과 한국 집단내 또는 집단간 절편 양상의 차이도 관찰할 수 있었다. To investigate the population differences of small yellow croaker (Pseudosciaena polyactis BLEEKER) in the Yellow Sea, five catching sites (three from China; Zoushan, Shanghai and Qingdao, two from Korea; Inchon and Mokpo) were selected for sampling. The populations of small yellow croaker from all five catching sites were investigated to analyze their mtDNA's restriction fragment length polymorphism (RFLP) using 18 kinds of restriction enzymes. The average molecular size of the entire mtDNA was estimated at $16.9{\pm}0.6\;kb$. According to the results of RFLP analysis, a total of 40 restriction sites were identified in every population surveyed and the overall cleavage patterns of mtDNA, based on the RFLP, showed similar tendencies. However, the five restriction enzymes such as ApaI, EcoRI, PstI, SmaI and SstII showed slightly different cleavage patterns which could have resulted from individual variations between the populations of Korea and China.

폐쇄성수면무호흡을 유발한 후인두 지방육종 1예

황규린,강은구,이용만,오천환 대한이비인후과학회 2008 대한이비인후과학회지 두경부외과학 Vol.51 No.6

Although liposarcomas are second most frequently encountered sarcomas that occur in soft tissue, liposarcomas of the head and neck are very uncommon. Liposarcomas of the retropharyngeal space are rare and do not cause symptoms until they reach a large size to cause mass effect. Depending on the location of the pharyngeal tumor, patients may present with foreign body sensation on throat, swallowing difficulty, respiratory difficulty, a painless neck mass, or obstructive sleep apnea. Recently we have experienced a case of retropharyngeal liposarcoma causing obstructive sleep apnea, so we report our clinical experiences with a brief review of literature. (Korean J Otorhinolaryngol-Head Neck Surg 2008;51:577-80)

Preliminary Analysis of Molecular Biological Methods for Stock Identification of Small Yellow Croaker(Pseudosciaena polyactis) in the Yellow Sea

허회권,황규린,인영철,장정순,HUE Hoi-Kwon,HWANG Gyu-Lin,LEE Yong-Chul,CHANG Chung-Soon The Korean Society of Fisheries and Aquatic Scienc 1992 한국수산과학회지 Vol.25 No.6

황해서식 참조기(Pseudosciaena polyactis)에 대한 계군을 분석하기 위한 연구의 일환으로 우선 황해의 목포 연근해에서 채집된 참조기의 난자와 근육으로부터 mt-DNA와 근육 actin을 추출하였다. 난자의 경우 mt-DNA를 추출한 뒤 제한효소로 처리하여 절편다형현상을 관찰하였으며 근육 actin의 경우 단백질 분해효소(Staphylococcus aureus $V_8$ protease)로 처리하여 N-terminal 절편의 다형현상을 각각 관찰하였다. Mt-DNA의 genome 크기는 약 $\16\pm0.2$ Kb였고 전체 절편수는 37개였으며 사용된 20개의 제한효소 중 8개의 제한효소만이 3개 정도의 절편을 보이므로써 유의성을 관찰할 수 있었다 한편 근육 actin을 Staphylococcus aureus $V_8$단백질 분해효소로 처리하였을 때 N-terminal 부위에서 26 KDa 및 16 KDa의 분자량을 갖는 특이절편을 관찰할 수 있었다. The stock identification of small yellow croaker. Pseudosciaena Polyactis from Mokpo area was carried out using molecular biological methods such as mt-DNA restriction fragment length polymorphism(RFLP) and the N-terminal fragment polymorphism of muscle actin obtained after protease digestion. The entire mt-DNA genomic size from the small yellow croaker at Mokpo area was estimated to be about $16\pm0.2$ Kb. Furthermore, fourteen restriction endonucleases revealed a total of 37 restriction sites to the mt-DNA molecule, however, eight of the fourteen enzymes showed a significant restriction site variation. Six of the enzymes examined produced a single restriction profile for all individuals surveyed, indicating that they don't react on the same mt-DNA obtained from small yellow croaker. The Staphylococcus aureus $V_8$ protease is able to cleave the muscle actin of small yellow croaker and to yield a N-terminal peptide of 26 and 16 KDa, respectively.

Surgical Results of Endoscopic Dacryocystorhinostomy and Lacrimal Trephination in Distal or Common Canalicular Obstruction

백병준,황규린,정동호,김이석,신재민,이흥만 대한이비인후과학회 2012 Clinical and Experimental Otorhinolaryngology Vol.5 No.2

Objectives. To evaluate the surgical outcomes of endoscopic dacryocystorhinostomy followed by canalicular trephination and silicone stenting in patients with distal or common canalicular obstructions. Methods. The medical records of 29 patients (31 eyes) from January 2001 to December 2009 who underwent endoscopic dacryocystorhinostomy followed by canalicular trephination and silicone tube insertion for the treatment of distal or common canalicular obstructions were retrospectively reviewed. The level of obstruction was confirmed by intraoperative probing. The outcome of the surgery was categorized as a complete success, partial success, or failure according to the functional and anatomic patency. Results. The average age of the patients was 52 years. The duration of silicone intubation ranged from 4 to 11 months with an average of 5.7±1.6 months. The follow-up period after stent removal ranged from 4 to 15 months with an average of 8.2±3.3 months. Complete success was achieved in 25 out of 31 eyes (80.6%), partial success in 4 out of 31eyes (12.9%), and failure in 2 out of 31 eyes (6.5%). Conclusion. Endoscopic dacryocystorhinostomy followed by canalicular trephination and silicone stent intubation may be safe and considered as an initial treatment of patients with distal or common canalicular obstructions

중이이식장치

최재영,황규린 한양대학교 의과대학 2015 Hanyang Medical Reviews Vol.35 No.2

Patients with hearing loss have been increasing according to the extension of lifespan. Recently, new technology which solves the limitation of conventional hearing aids has been developed. For example, the active middle ear implantation which can directly transfer the vibration energy to the ossicles and inner ear fluid can avoid the feedback and occlusion effect, because this device uses the electromagnetic transducer, not a microphone. Active middle ear implants give more clear sounds and more gain in high frequency area. This device can apply to the patients with ski-sloping hearing loss and severe mixed hearing loss. In the paper, we reviewed the benefits and ideal candidates of the middle ear implant.

온도안진검사상 정상 소견을 보이는 선택적 하전정신경염 1예

강은구,황규린,이종대,성기범 대한평형의학회 2009 Research in Vestibular Science Vol.8 No.1

Vestibular neuritis is commonly diagnosed by demonstrating of peripheral vestibular failure as a unilateral loss of the caloric response. It is a sudden, spontaneous, unilateral loss of vestibular function without simultaneous hearing loss or brainstem signs. In most patients with vestibular neuritis, the process is thought to involve the superior vestibular nerve. Very rarely, vestibular neuritis involves only the inferior vestibular nerve. We experienced a 56-year-old male with inferior vestibular neuritis. The patient had vertigo and spontaneous nystagmus, but a normal caloric test. Brain magnetic resonance imaging was normal, while vestibular evoked myogenic potentials had absent amplitudes on the lesion side. The patient was thought to suffer from pure inferior nerve vestibular neuritis. Vestibular neuritis is commonly diagnosed by demonstrating of peripheral vestibular failure as a unilateral loss of the caloric response. It is a sudden, spontaneous, unilateral loss of vestibular function without simultaneous hearing loss or brainstem signs. In most patients with vestibular neuritis, the process is thought to involve the superior vestibular nerve. Very rarely, vestibular neuritis involves only the inferior vestibular nerve. We experienced a 56-year-old male with inferior vestibular neuritis. The patient had vertigo and spontaneous nystagmus, but a normal caloric test. Brain magnetic resonance imaging was normal, while vestibular evoked myogenic potentials had absent amplitudes on the lesion side. The patient was thought to suffer from pure inferior nerve vestibular neuritis.

양측 측두골 골절로 인한 농 환자에서의 인공와우이식

이종대,전수성,황규린,김시찬 대한이비인후과학회 2008 대한이비인후과학회지 두경부외과학 Vol.51 No.10

Transverse temporal bone fracture can make a profound sensorineural hearing loss. This temporal bone fracture usually happens in the single side;however, the bilateral temporal bone fracture can make the postlingual deafness. Therefore, the deafness from bilateral temporal bone fractures can be one of the indications for the cochlear implantation. We report on the experience of the cochlear implantation in a deaf patient after bilateral temporal bone fractures. (Korean J Otorhinolaryngol-Head Neck Surg 2008;51:917-20)