간척지 양파 생산 시 생육 및 기능성 성분 함량 비교

한지원,최민선,김지강 경상국립대학교 농업생명과학연구원 2023 농업생명과학연구 Vol.57 No.2

This study was carried out to evaluate the growth and bioactive compounds of different onion varieties in reclaimed land (Saemangeum)and Muan. Mid-maturing variety ‘Mapsihwang’, and mid-late maturing varieties, ‘Sunpower’, and ‘Healthy Q’ were used. Onions weretransplanted after seeding preparation in early November in Muan, Jeollanam-do, which is the main production region of onion andreclaimed land, respectively, and harvested in early June of the following year. The growth of mid-late maturing varieties ‘Sunpower’and ‘Healthy Q’ was good in reclaimed land with a bulb weight 336.5g, 248.3g, respectively. Pyruvic acid, when compared amongvarieties, ‘Healthy Q’, ‘Mapsihwang’ and ‘Sunpower’ were in the order, and depending on the region, the contents of ‘Healthy Q’ and‘Mapsihwang’showed 48.3μmol·g-1, 41.1μmol·g-1, respectively, when cultivated in reclaimed land. In the case of total quercetin, whencompared among varieties, ‘Healthy Q’was the highest with 219.1μg·g-1, followed by ‘Sunpower’ and ‘Mapsihwang’, but there was nosignificant difference in region. In addition, total phenol showed the similar trend as total quercetin, and the total phenol content of‘Healthy Q’ was the highest at 853.7μg·mL-1, and ‘Sunpower’ significantly high when cultivated in Muan. As a result, the content ofpyruvic acid, total quercetin, total phenol was found to have an effect depending on the variety than the cultivated region.

A Cross-cultural Study of Responses to Offensive Situations

한지원 서울대학교 언어교육원 2014 語學硏究 Vol.50 No.2

Although Korean and English speech act studies have increased inquantity, a closer look revealed some limitations from qualitativeperspectives. Many speech act studies employed similar data collectionthat draw on similar coding systems and investigated speech acts betweenKoreans as non-native speakers and native speakers of English. For this reason, similar findings were consistently reported from theliterature. This study examines two data sets collected from Koreansand Americans as native speakers. The study also examines diverse responsesoccurring in six different scenarios and considers cultural-socialfactors in the linguistic realization of apology. Results indicatecross-cultural differences are found in the use of non-apologies andnon-verbal behavior which are missing from the previous studies. Secondly, contextual factors like social relationships and offense typesare likely to affect the choice of language between Korean and Englishspeech apologies. The study concludes a substantial comparison needsto expand kinds of speech acts in order to understand interculturalcommunication.

Politeness in English : Based on chats communication

한지원 한국언어과학회 2011 한국언어과학회 학술대회 Vol.2011 No.08

This study analyzes chats conversation to understand how politeness is achieved in English conversation. Within Brown and Levinson’s model (1987), politeness is introduced as the concept based on the social notion of face and interpreted as the effort to avoid Politeness is interpreted as the effort to avoid a threatening act or to mitigate the threat if it is inevitable. This research is motivated by the author’s experiences with working through a native speaker of English and being aware of politeness. Academic interaction with her co-worker, an American speaker of English provides an opportunity to enhance the author’s awareness of communication as social interaction and face-involved. This study focuses on the native use of English and identifies features and strategies in order to see how politeness is achieved when face-threat has to be committed. Records of chats have been documented and analyzed to investigate the use of English in order to see what kinds of strategies are used and enhance the author’s awareness of politeness. The data selected for the current study come from chats conversation occurring for three months in 2007. Analysis of the data show that it is difficult to avoid threatening acts in verbal interaction. A set of face-threatening acts (FTAs) are identified such as disagreement, interruption, requests, and correction of English. The use of linguistic means shows how politeness can be achieved from a native speaker’s perspective.

Effects of Nutritional Input and Diesel Contamination on Soil Enzyme Activities and Microbial Communities in Antarctic Soils

한지원,정재준,현승훈,박현,박우준 한국미생물학회 2012 The journal of microbiology Vol.50 No.6

Pollution of Antarctic soils may be attributable to increased nutritional input and diesel contamination via anthropogenic activities. To investigate the effect of these environmental changes on the Antarctic terrestrial ecosystem, soil enzyme activities and microbial communities in 3 types of Antarctic soils were evaluated. The activities of alkaline phosphomonoesterase and dehydrogenase were dramatically increased, whereas the activities of β-glucosidase, urease, arylsulfatase, and fluorescein diacetate hydrolysis were negligible. Alkaline phosphomonoesterase and dehydrogenase activities in the 3 types of soils increased 3- to 10-fold in response to nutritional input, but did not increase in the presence of diesel contamination. Consistent with the enzymatic activity data, increased copy numbers of the phoA gene, encoding an alkaline phosphomonoesterase, and the 16S rRNA gene were verified using quantitative real-time polymerase chain reaction. Interestingly, dehydrogenase activity and 16S rRNA gene copy number increased slightly after 30 days, even under diesel contamination, probably because of adaptation of the bacterial population. Intact Antarctic soils showed a predominance of Actinobacteria phylum (mostly Pseudonorcarida species) and other phyla such as Proteobacteria, Chloroflexi, Planctomycetes, Firmicutes, and Verrucomicrobia were present in successively lower proportions. Nutrient addition might act as a selective pressure on the bacterial community, resulting in the prevalence of Actinobacteria phylum (mostly Arthrobacter species). Soils contaminated by diesel showed a predominance of Proteobacteria phylum (mostly Phyllobacterium species), and other phyla such as Actinobacteria, Bacteroidetes, Planctomycetes, and Gemmatimonadetes were present in successively lower proportions. Our data reveal that nutritional input has a dramatic impact on bacterial communities in Antarctic soils and that diesel contamination is likely toxic to enzymes in this population.

Perception of (im)politeness in Korean apology

한지원 사단법인 한국언어학회 2012 언어학 Vol.0 No.63

Jee-Won Hahn. 2012. Perception of (im)politeness in Korean apology. The purpose of the study was to identify factors that affect the perception of politeness in the act of apologizing. A perception task has been employed for Korean apologies. The potential apologies are presented to native speakers of Korean in order to see how such utterances are perceived by native speakers. Responses to apology events are analyzed in two ways: quantitatively and qualitatively. The choice of apologies over non-apologies is calculated for their perception of politeness. This study analyzed three aspects: apology strategies, use of performatives, and situational variables. The stimuli used for the perception task were selected based on data from a previous study. A total of ten items were used in order to elicit responses from Korean native speakers. The degree of politeness was calculated based on how the given apology item had been interpreted. If the items were perceived to be sincere and appropriate for the context, participants were likely to choose apologies over non-apologies and vice versa. Such choices are presented in percentages for politeness. This study demonstrates the role of linguistic strategies and performatives in apologies. The choice of linguistic strategies is found to affect the degree of politeness that people perceive. Among others, different strategies are classified as other-oriented politeness. Furthermore, the degree of politeness also varies according to the presence of performatives. Additional findings come from metalinguistic views on apologetic utterances. .Native interpretations of apologetic utterances provide comments that reflect views on situational variation.

EXPRESSING DISPLEASURE IN KOREAN: ON MITIGATION AS A SOCIO-CULTURAL FACTOR

한지원 사단법인 한국언어학회 2009 언어학 Vol.0 No.54

As a sequence on an offensive situation, this study analyzes the act of expressing displeasure while the author’s previous research was apologizing. It examines how the universal theory of politeness accounts for Korean and what implications are involved with theories. According to this study, displeasure is expressed through several forms such as mentioning the offense, stating obligation, or giving a directive. The choice of linguistic strategies shows similarity with other languages while a cross-cultural difference is found in the way of mitigating a face-threatening act. A common strategy of indirectly stating offense or obligation is the use of interrogative sentence in Korean. Being indirect is claimed as a universal feature for threatening acts in politeness theory (Brown and Levinson 1987; Goffman 1971). However, universal claims from a theoretical perspective may not apply cross-culturally. A theory needs to be complemented by socio-cultural interpretation. For methodology, the data was collected from sequential research on verbal behavior occurring in offensive situations. To understand politeness in Korean, it takes into consideration linguistic behavior in the discourse level beyond the sentence level. The discourse-level analysis comes from observation-based speech. Controlled data collected through written questionnaires or role-plays tend to be limited to a single turn. Naturally-occurring data make it possible to look at contextual factors and social relationships between two parties.

한-아프리카 농식품 기술협력협의체(KAFACI) ‘토마토 및 양파 생산성 향상 기술개발’ 과제 수행현황 및 에티오피아 농업현장 소개

한지원,정효봉,조명철,권영석,이정란 한국종자연구회 2023 종자과학과 산업 Vol.19 No.2

Inhibition of HIF1α and PDK Induces Cell Death of Glioblastoma Multiforme

한지원,임평원,나철민,최유식,이주영,김요나,박형우,문효은,허만승,박혜란,김동규,백선하 한국뇌신경과학회 2017 Experimental Neurobiology Vol.26 No.5

Glioblastoma multiforme (GBM) is the most common and aggressive form of brain tumors. GBMs, like other tumors, rely relatively less on mitochondrial oxidative phosphorylation (OXPHOS) and utilize more aerobic glycolysis, and this metabolic shift becomes augmented under hypoxia. In the present study, we investigated the physiological significance of altered glucose metabolism and hypoxic adaptation in the GBM cell line U251 and two newly established primary GBMs (GBM28 and GBM37). We found that these three GBMs exhibited differential growth rates under hypoxia compared to those under normoxia. Under normoxia, the basal expressions of HIF1α and the glycolysis-associated genes, PDK1, PDK3, and GLUT1, were relatively low in U251 and GBM28, while their basal expressions were high in GBM37. Under hypoxia, the expressions of these genes were enhanced further in all three GBMs. Treatment with dichloroacetate (DCA), an inhibitor of pyruvate dehydrogenase kinase (PDK), induced cell death in GBM28 and GBM37 maintained under normoxia, whereas DCA effects disappeared under hypoxia, suggesting that hypoxic adaptation dominated DCA effects in these GBMs. In contrast, the inhibition of HIF1α with chrysin suppressed the expression of PDK1, PDK3, and GLUT1 and markedly promoted cell death of all GBMs under both normoxia and hypoxia. Interestingly, however, GBMs treated with chrysin under hypoxia still sustained higher viability than those under normoxia, and chrysin and DCA co-treatment was unable to eliminate this hypoxia-dependent resistance. Together, these results suggest that hypoxic adaptation is critical for maintaining viability of GBMs, and targeting hypoxic adaptation can be an important treatment option for GBMs.

메타분석을 활용한 한국어 교육에서의 구성주의 기반 교수․학습 전략의 효과 연구

한지원 언어과학회 2019 언어과학연구 Vol.0 No.91

The student-centered learning environment based on constructivism has been around since the early 2000s. However, it has been frequently reported that these principles, especially in the context of Korean Language Education (KLE), are rarely implemented in practice due to the burden of various exams and tasks. In this study, it is first claimed that the student-centered learning environment based on constructivism is indeed the major factor for a successful learning in the context of KLE. To support this claim, meta-analysis is introduced to calculate ‘effective size’ which allows for a quantitative comparison of the constructivism-based and the traditional teaching methodologies for KLE. As a result, it is shown that the constructivism-based teaching methodology is indeed more effective in the practical domain of KLE except speaking, i.e. grammar, listening, writing, vocabulary, and reading, than the traditional one.

코딩놀이 과정에서 나타난 유아의 메타인지 경험

한지원 한국어린이미디어학회 2022 한국어린이미디어학회 학술대회 자료집 Vol.2022 No.6

본 연구에서는 메타인지의 교육적 가치를 모색하기 위해 코딩놀이에서 유아가 겪은 메타인지 경험을 분석하였다. 만 5세 유아를 대상으로 2021년 4월 5일부터 8월 27일까지 21주 동안 비스킷을 활용한 코딩놀이가 이루어졌다. 연구 수행과정에서 유아의 반응과 상호작용을 기록한 참여관찰, 면담, 연구일지 등의 자료를 수집하였으며, 분석에 활용하였다. 코딩놀이 과정에서 유아가 겪은 메타인지 경험은 다음과 같이 분류할 수 있었다. 첫째, 나의 행동을 인식하는 경험, 둘째, 사고과정을 점검하는 경험, 셋째, 놀이에 대해 평가하는 경험 등이다. 본 연구는 유아에게도 메타인지 능력이 존재한다는 점과 메타인지가 유아의 자발적인 동기에 의해 활동을 선택하고 그 속에서 더 깊은 배움이 일어나게 하는 계기가 될 수 있다는 점을 심도 있게 보여주었다는데 의의가 있다. 본 연구의 결과는 코딩놀이를 실행하는 과정에서 유아에게 의미 있는 메타인지 경험을 제공하기 위한 자료로 활용될 수 있을 것이라 기대한다.