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영지의 ${\beta}$-glucan성 다당류에 의해 활성화된 흰쥐 간내 Kupffer 세포의 NO, TNF-${\alpha}$ 및 TGF-${\beta}$ 형성
한만덕,이준우,정훈,김용석,나수정,윤경하,Han, Man-Deuk,Lee, June-Woo,Jeong, Hoon,Kim, Yong-Seok,Ra, Su-Jung,Yoon, Kyung-Ha 한국미생물 · 생명공학회 1999 한국미생물·생명공학회지 Vol.27 No.1
Ganoderan (GAN), an immunomodulating ${\beta}$-glucan from mushroom Ganoderma lucidum, was evaluated for its ability to induce formation of nitric oxide (NO), tumor necrosis factor-${\alpha}$(TNF-${\alpha}$) and transforming growth factor (TGF-${\beta}$) from rat Kupffer cell in vitro. Hepatic macrophages activated by GAN significantly elevated concentration of NO and TNF-${\alpha}$ in cultured medium, but not significantly elevated that of TGF-${\beta}$. GAN-activated Kupffer cells secrete 14.9${\mu}$M (p<0.01) of NO and 2619.5${\rho}$g/ml (p<0.01) of TNF-${\alpha}$after 36hr of incubation at 37$^{\circ}C$. The results revealed that GAN enhanced 4-fold production of NO and 19 fold formation of TNF-${\alpha}$ compared to the control. The proliferation of GAN-activated Kupffer cells was inhibited as compared with its negative control. Comparing the activity among glucans derived from microorganisms, highly branched zymosan, glucomannan from Saccharomyces cerevisiae, significantly increased TNF-${\alpha}$ and NO production. These results indicate that the ${\beta}$-glucan from G. lucidum activates rat Kupffer cell and secretes NO and TNF-${\alpha}$. It also suggest that rat Kupffer cell posses certain receptor for ${\beta}$-anomeric glucan.
영지(Ganoderma lucidum)의 β-Glucan에 의한 Sarcoma-180 육종암 생장 억제
한만덕(Man-Deuk Han),김용현(Yong Hyun Kim),김완종(Wan Jong Kim) 한국생명과학회 2014 생명과학회지 Vol.24 No.7
버섯 다당류 β-glucan의 항암 활성을 확인하기 위하여 영지균사체로부터 단백다당류(GLP)를 분리하고 Sarcoma-180 육종암을 이식시킨 마우스에 복강 투여하여 항암활성을 확인하였다. 육종암이 서혜부에 이식된 마우스에 GLP를 20 mg/kg의 농도로 10일간 복강투여 한 후 30일차에 확인한 결과, Sarcoma-180 육종암은 대조군 대비 71.4% 억제되었으며, 마우스의 혈청, 종양조직 및 간조직 내의 TNF-α의 농도는 대조군보다 높게 나타났다. 따라서 GLP는 생체 내 TNF-α의 양적증가를 유도하며, 종양괴사 또는 에폽토시스와 연관된 육종암의 생장억제가 확인되었다. 이때 생장이 억제된 육종암 세포의 미세구조를 관찰한 결과, 상대적으로 큰 핵과 세포의 에폽토시스에서 전형적으로 보여지는 염색질 응축이 관찰되었으며, 핵막은 특징적으로 뭉쳐져 불규칙한 모양을 나타내었다. 따라서 영지에서 분리된 GLP는 종양 세포의 에폽토시스를 유도하여 종양의 성장을 억제하는 것으로 여겨진다. Mushroom-derived β-glucan, a polysaccharide (GLP) isolated from the mycelium of Ganoderma lucidum, was previously shown to have inhibitory effects against tumor-bearing mice in vivo. We investigated the apoptotic effect of mushroom-derived β-glucan in a sarcoma-180 tumor cell- bearing mice model using an ELISA to determine the levels of tumor necrosis factor-α (TNF-α) in the mice. The morphology of the tumor cells was assessed with transmission electron microscopy (TEM). GLP was injected into the tumor-bearing mice at a dose (i.p.) of 20 mg/kg for 10 days. After 30 days, the tumor mass from the inguinal region was collected, weighed, and assayed using TEM and a TNF-α ELISA kit. The tumors that developed in the mice treated with GLP were 71.4% smaller than those in the control group, showing the ability of GLP to inhibit tumor growth. The levels of TNF-α in the serum of the sarcoma-180 bearing mice were 12 times greater than in the serum of the nonbearing tumor mice. An ultrastructural study demonstrated that the GLP-treated sarcoma-180 tumor cells were condensed, with rearranged chromatin. In addition, the marginated chromatin in nucleus induced the nuclear compartment, and there were many vacuolization in the cell. GLP could be an effective apoptosis-inducing compound in sarcoma-type cancers.
잎새버섯(Grifola frondosa)균사체의 기능성 다당류 최적 추출방법 및 항암효과
한만덕 ( Man Deuk Han ) 한국식품영양학회 2012 韓國食品營養學會誌 Vol.25 No.1
Grifola frondosa has been used as an herbal medicine for the treatment of cancer, diabetes mellitus and high blood pressure. In this study, functional polysaccharide was obtained from Grifola frondosa using four different extraction methods: hot water(HwFP), homogenize(HgFP), acid(AcFP), and alkali(AlFP) extraction methods. The effects of these extracts on KB and HepG2 cell lines were then examined for any anti-cancer activity. Alkaline extraction produced a yield of 0.175% and the total sugar content of the extract was 54.97%. We were able to confirm that the polysaccharide extracts from the mushroom produce an anti-cancer effect. The cytotoxicity of AlFP and AcFP against HepG2 cells were 22.86% and 28.88%, respectively, and the cytotoxicity of AlFP against the KB cell lines was 47.76% at a concentration of 1,000 ㎍/㎖. Therefore, these results suggest that the optimum method for extracting functional polysaccharides from G. frondosa is the alkali extraction method.