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金永權 中央醫學社 1968 中央醫學 Vol.14 No.6
The bacteriological characteristics and the pathogenicity of enteropathogenic halophilic vibrios isolated from sea-fishes and shell-fishes collected in the port of Pohang, Kyungpook during July and August of 1966 and 1967 were studied, and the following results were obtained. 1. Vibrios were classified into three species, i.e. Vibrio parahaemolitycus, V. alginolyticus, and V. anguillarum. The criteria of classification were based on the growth on nutrient agar containing 7 and 10 per cent of sodium chloride, Voges-Proskauer reaction, swarming growth on nutrient agar, and fermentation of sucrose and cellobiose. 2. The biological characteristics of vibrios were, in general, uniform among strains in each. species, and three species of vibrios showed their typical characteristics in each species.. 3. Most of test organisms can grow well in trypticase soy broth of pH 4.5-5.0, and there were a few strains which can grow in media of pH 6. 0 4. Most strains were resistant to penicillin, methicillin, and cloxacillin, and grow well in the presence of 200-100u or mcg/ml of these antibiotics. The resistance to streptomycin and erythromycin was moderate and the minimum inhibitory concentration (M.I.C.) was 50 mcg/ml or more in most strains. However, the sensitivity to chloramphenicol, aureomycin, terramycin, and tetracyline was very high with the M.I.C. of 1.6mcg/ml, and there were some strains with the M.I.C. of 3.1-6. 2mcg/ml including a few strains with the M.I.C. of 6.2mcg/ml. 5. One-half to one-fourth ml of broth culture containing 3.8-8.3X108/ml of viable cells were injected into the peritoneal cavity of mouse, and the death was observed.
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金榮權,柳逢夏,朴東源,柳基遠 대한한방종양학회 1998 대한한방종양학회지 Vol.4 No.1
Yookmijihwangtang has been widely used oriental herb prescriptions, which is healing some discuss that come from insufficiency of innate essence and deficiency of kidey Ki. the meaning of healing discusses tonification of insufficient innate essence and insufficient kidney Ki can be regarded as reinforcement of wholely power of keeping homeostasis, that is correlated with immuno-responsibility which protects subject from outer antigen to keep normal vital condition. This study was aimed to investigate correlative effects of Yookmijihw angtang water abstract on the RBC, WBC, blood CD4+ Theler cell count, blood testosterone, blood cAMP and blood cortisol. 40 Sprague-Dawley male rats were divided into 5 groups(Normal, Control, SampleⅠ, SampleⅡ, SampleⅢ), 6 animals in every group. Normal group was not treated anything, control group was administrated normal saline in the same desage of sample Ⅰ. 3 Sample groups were received some of Yookmijihwangrang water abstract at one time per 24 hours during 5 days in different dosage. Sample Ⅰ (1/3 10pack/ml), SampleⅡ (1/62pack/ml), SampleⅢ(1/2.4pack/ml). After finishing treatment, all experimental svbjects were killed for blood sample on RBC, WBC, blood CD4+ T helper cell count, spleen CD4+ T helper cell count, axillary lymph node CD4+ T helper cell count. .blood cAMP, blood testosterone and blood cortisol. The results were as follows: RBC and WBC were incrased in all sample groups. Blood CD4+ Thelper cell count(CD4+ T cell count in the blood/whole lymphocyte count in the blood ×100%) was Mormal 46.17±5.88. Control 44.50±4.37, Sample Ⅰ 53.00±2.28. Sample Ⅱ 53.83±3.87, SampleⅢ 52.17±2.93. By the 95% Duncan ANOVA all experimental groups(sampleⅠ, sampleⅡ, sampleⅢ) showed slight significant difference from Nornal and Control. Blood cAMP(nmd/l) were Normal 1.12±0.17, Control 1.16±0.32, SampleⅠ 0.46±0.07, SampleⅡ 0.44±0.04, SampleⅢ 0.54±0.04 All cxperimental groups were signigicantly different from both Normal and Control groups(p<0.05). Blood cortisol(m l/ml) were Normal 100.00±2.00, Control 90.00±4.00, Samplc Ⅰ 440.00±5.00, SampleⅡ 520.00±40.00, SampleⅢ 470 00±7.00, Blood cortisol of all experimental groups were signifieantly increased(p<0.05). The results suggest that Yookmijihwangiang water abstract could be administrated to patienrts who have some diseases insufficient essence.
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