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崔大卿,張明雄,朴鼎圭 충남대학교 의과대학 지역사회의학연구소 1982 충남의대잡지 Vol.9 No.1
For the improvement of detection of leprosy bacilli from patient, author's attention of the bacterial detection ratio have been devoted to compared the skin smear method with the nuclepore filter method from 200 patients who were registrated in Chungnam Skin Clinic of Korean Leprosy Association. One hundred patients out of 200 registered were positive in bacterial detection, the others were negative. For the dispersion of bacterial globi in nuclepore filter method, the skin scrapped materials were treated with 0.025% trypsin and 0. 002 % tween 80 for 30 minutes at 37℃ incubator. The efficiency of bacterial detection between the nuclepore 13 mm diameter of the filter and the 25 mm filter were investigated in the broth cultured M. fortuitum. The cultured M. fortuitum was transferedl ml into each 10 tubes, and then was carried out ten-fold dilution, respectively. The specimen for comparative counting of acid fast bacilli were made on 10^-4 dilution in using 25 mm diameter of filter and 10^-5 dilution in using 13 mm diameter of filter and cultural method. The results were summarized as follows: 1. The ratio of bacterial detection by the skin smear and nuclepore filter methods from 100 patients who were registrated with bacterial detection positive cases were not observed any difference, in all cases were detected leprosy bacilli with both methods. 2. In 100 patients who were registrated with bacterial detection negative, 19 patients were detected leprosy bacilli by the skin smear method and 33 patients were detected leprosy bacilli by the nuclepore filter method. The ratio of bacterial detection by two methods were observed statistically significant difference. (0.01<p<O. 05). 3. Between bacterial indices by skin smear and bacterial numbers of nuclepore filter methods were observed perfect positive corelation. 4. There was close corelation between 25 mm filter and the culture method, however, 13 mm filter was more higher than other two methods. From the above results, it was suggested that the nuclepore filter method with well digesting specimens with 0.025% trypsin and 0.002% tween 80 would be more accurate and higher detection ratio of M. leprae than skin smear method. The nuclepore 13mm filter was more efficient to detection of acid fast bacilli than 25 mm filter.
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오리 선위 점액분비세포의 점액질에 대한 조직화학적 연구
류시윤,김홍선,김무강,신태균,이영호 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.2
Histochemical sequential staining techniques, PAS, alcian blue pH 2.5 and pH 0.4, were applied to sections from the proventriculus of ducks to observe the histochemical properties of epithelial mucosubstances in the proventriculus of mallard and white Pekin ducks. The whole proventriculus obtained were divided systemically cross strips and processed routinely for histologic and histochemical study. Base on the histologic characteristics, we designated as upper portion which is the distal region of the esophageal-proventriculus junction, as middle portion, and as lower portion which is the proximal region of the proventricular-isthmus junction. The results obtained are as follows 1. Generally in the surface and foveolar epithelia showed the presence of the mixture of neutral and acid mucosubstances. 2. In the tubular gland showed the acidic nonsulfated and sulfated mucosubstance in addition to neutral mucosubstances with occasional neutral and acidic nonsulfated mucosubstances. 3. In the compound gland neutral mucosubstance with scant amounts of acidic mucosubstances or with lack of it were demonstrated. 4. There were recognized differences between speies in the staining intensity of all three types of mucosubstances. 5. There were recognized topographic difference in the acidity of epithelial mucosubstances according to portion.
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HMG-CoA 환원효소 억제제에 의한 ICAM-1 유전자의 발현조절
김현진,정효균,홍우정,김군순,조영석,김도희,채수흥,구본정,송민호,노흥규,김영건 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1
Background : ICAM-1 act as one of major adhesion molecules in the atherosclerotic lesion. ICAM-1 expression is mainly regulated at the level of transcription and depend on IFN-γ signal transduction pathway in which the STAT1 transcrption factor is a critical intermediate. IFN-γreceptor not only initiates tyrosine 701 phosphorylation of STAT1 by Jak1 and Jak2, but also phosphorylates serine 727 through the activation of Raf-1/MAP kinases. HMG-CoA reductase inhibitors have anti-atherosclertic effects, beyond normalization of hypercholesterolemia, by directly acting on endothelial cells, macrophages and vascular smooth muscle cells. HMG-CoA reductase inhibitors suppress the synthesis of isoprenoid intermediates such as geranylgeranyl-pyrophosphate or farnesylpyrophosphate. These effects results inhibitors suppress the synthesis of isoprenoid intermediates such as geranylgeranyl-pyrophosphate or farnesylpyrophosphate. These effects results inhibition posttranslational farnesylation and geranyl-geranylation processing of small GTP-binding preoteins and inhibition of normal signaling activities. Method : We made several 5'-deletion constructs of rat ICAM-1 promoter and analyzed the promoter activities by measuring the luciferase activity after transfection into ECV304 cells and smooth muscle cells. We checked the level of total and phosphorylated STAT1 protein by immunoblot analysis using specific antibodies. Results : Lovastatin inhibits IFN-γ-induced ICAM-1 gene expression in the ECV304cell. The cells pretreated with PD98059, MEKK inhibitor showed significantly low ICAM-1 RNA induction with IFN-γ stimulatio. IFN-γ induced phosphorylation of tyrosine 701 was not significantly changed by the pretreatment of lovastatin. But lovastatin suppresses IFN-γ-induced phosphorylation of ERK1/ERK2 which are responsible for the seine 727 phosphorylation in STAT1. Conclusion : We showed that HMG-CoA reductase inhibitors, lovastatin, suppresses IFN-γ mediated ICAM-1 gene expression through the inhibition of transcription. HMG-CoA reductase inhibitor suppresses IFN-γ-induced phosphorylation of serine 727 in STAT1 through the modulation of MAP kinases.
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