Intralipidos에 대한 변이원성시험

정지윤,이원우,임종희,남정석,제정환,이광훈,강병철,이병희,박재학,이영순,Jung, Ji-Youn,Lee, Won-Woo,Ihm, Jong-Hee,Nam, Jeong-Seok,Che, Jeong-Hwan,Li, Guang-Xun,Kang, Byeong-Cheol,Yi, Beoung-Hi,Park, Jae-Hak,Lee, Yong-Soon 한국독성학회 1998 Toxicological Research Vol.14 No.3

In order to evaluate the mutagenic potential of Intralipidos produced by Greenmate cooperation. We performed Salmonella typhimurium reversion assay, chromosomal aberration test on chinese hamster ovarian cells and in vivo micronucleus assay using mouse bone marrow cells. In the reverse mutation test using Salmonella typhimurium TA98 and TA100, Intralipidos did not increase the number of revertant at any of the concentration tested in this study. Intralipidos did not increase the number of cells having structural or numberical chromosome aberration in cytogenetic test. In mouse micronucleus test, no significant increase were observed in the occurrence of micornucleated polychromatic erythrocytes in ICR male mice intraperitoneally administered with Intralipidos. These results indicate that Intralipidos has no genetic toxicity under these experimental conditions.

실험동물에서의 환경호르몬 물질의 생체내 영향 및 검색법 정립에 대한 연구

정지윤,이영순,Jung, Ji-Youn,Lee, Yong-Soon 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.4

The major protocol features of the rodent uterotrophic assay have been evaluated using a range of reference chemicals. The protocol variables considered include the selection of the test species and route of chemical administration, the age of the test animals, the maintenance diet used, and the specificity of the assay for estrogens. The rodents were ovariectomized under general anesthesia via bilateral flank incisions and randomly assigned to groups of 5 animals. Chemicals were DEHP, DBP, BPA and NP, were injected sc once daily with combinations of chemicals treatments for 3 days. In the results, the reported estrogenic chemicals DEHP and DBP were both negative in the single dose treatments. But, in the combinations of chemicals treatments, DEHP and DBP increased in bud number of mammary gland. Treatment of ovariectomized mice with combinations of other chemicals resulted in uterine and vaginal hyperplasia. The additive estrogenic effects were seen with the combinations of $17{\beta}$-Bestradiol and DBP treatment. the competitive estrogenic effects were seen with the combinations of $17{\beta}$-Bestradiol and nonylphenol, $17{\beta}$-Bestradiol and bisphenol-A treatments. These results offers a sysmatic and mechanistically informative approach to assessing estrogenicity. it provides a useful profile of activity using a reasonable amount of resources and is compatible with the study of individual chemicals as well as the investigation of interactions among combinations of chemicals. The results described illustrate the intrinsic complexity of evaluating chemicals for estrogenic activities and conform the need for rigorous attention to experimental design and criteria for assessing estrogenic activity.

시금치종자의 ${\alpha}-glucosidase$에 의한 L-ascorbic acid로부터 ascorbic acid-2-glucoside의 생산

정지윤,송희상,방원기,Chung, Ji-Youn,Song, Hee-Sang,Bang, Won-Gi 한국응용생명화학회 2004 Applied Biological Chemistry (Appl Biol Chem) Vol.47 No.2

Ascorbic acid로부터 $2-O-{\alpha}-D-glucopyranosyl-L-ascorbic$ acid(ascorbic acid-2-glucoside, AA-2G)를 생산하기 위하여, transglucosylation 활성을 가지는 시금치 종자의 ${\alpha}-glucosidase$를 효소원으로 이용하였다. 시금치 종자로 사용한 Spinachia oleracea L. WooSung의 조효소액의 ${\alpha}-glucosidase$ 활성은 발아 후 3일 째에 가장 높게 나타났으며, AA-2G의 생산은 2일 키운 시금치의 조효소액을 이용하였을 때, 생산량이 1.053 mM로 가장 높았다. 조효소액을 이용한 AA-2G 생산에 있어서 glucose 공여체로는 maltose가 가장 좋았으며, maltose와 ascorbic acid의 최적 농도는 각각 225 mM과 175 mM이었다. ${\alpha}-glucosidase$는 60 unit를 사용했을 때 생산량이 가장 좋았다. 효과적인 반응완충용액은 sodium acetate 완충액이었으며, 최적 농도는 175 mM이었다. 최적 pH및 반응온도는 각각 5.0과 $65^{\circ}C$였고, 최적 반응조건 하에서 50분 반응 후에 ascorbic acid로부터 2.30 mM의 AA-2G가 생산되었다. For the production of $2-O-{\alpha}-D-glucopyranosyl-L-ascorbic$ acid (ascorbic acid-2-g1ucoside, AA-2G) from ascorbic acid, the usability of spinach seed as the source of ${\alpha}-glucosidase$ having transglucosylation activity was studied. The optimum conditions for the production of AA-2G from ascorbic acid and glucose donor were investigated by using crude extract of Spinachia oleracea L. Woosung, the selected source of enzyme. The production of AA-2G was the highest with 1.053 mM when spinach seeds were grown for 2 days after germination. Maltose was the most effective glucose donor, and the optimum concentration of ascorbic acid and maltose were 175 mM and 225 mM, respectively. The optimum concentration of ${\alpha}-glucosidase$ was 60 units. The most effective buffer was sodium acetate and its optimum concentration was 175 mM. The optimum pH and temperature were 5.0 and $65^{\circ}C$, respectively. Under the optimum condition, 2.14 mM of AA-2G was produced from ascorbic acid after 50 minutes of reaction.

면역기능 증강 신물질에 대한 마우스의 면역학적 및 혈액학적 변화

정지윤,Jung, Ji-Youn 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.4

Immu-Forte composed of chitosan, ${\beta}-glucan$, manno-oligosaccharide and pangamic acid was evaluated for its effectiveness as a nonspecific immunostimulator in mice. The effects of Immu-Forte were determined by analysis of cytokines using ELISA and phenotype of leukocyte subpopulations using monoclonal antibodies specific to mouse leukocyte differentiation antigens and flow cytometry. All T cells, all B cells, CD4 T cells, CD8 T cells, macrophages, IL-2, IL-4, IL-12 and IFN-r in Immu-Forte A-treated group increased in 1 months posttreatment and were significantly higher (p < 0.05) than that of control at 1 months posttreatment. All T cells, all B cells, CD4 T cells, CD8 T cells, macrophages and IL-2 in Immu-Forte EX-treated low and middle dose groups increased in 1 months posttreatment and were significantly higher (p < 0.05) than that of control at 1 months posttreatment. In the Immu-Forte soybean-treated group, NK cells and IL-4 were significantly higher in middle dose-treated group, and IL-2, IL-4 and IFN-r were significantly higher in low dose-treated group. In the Immu-Forte F-treated group, all T cells, all B cells, CD4 T cells, CD8 T cells, macrophages, NK cells, IL-2, IL-4, IL-12 and IFN-r in high dose-treated group and all T cells, all B cells, CD4 T cells, CD8 T cells, macrophages, IL-2, IL-4, IL-12 and IFN-r in middle dose-treated group and NK cells, IL-2, IL-4, IL-12 and IFN-r in low dose-treated group were significantly higher (p < 0.05) than that of control at 1 months posttreatment. In conclusion, this study has demonstrated that Immu-Forte had an immunostimulatory effect on mice through proliferation and activation of mouse immune cells.

기획특집 : 구제역

정지윤 ( Ji Youn Jung ) 한국식품위생안전성학회 2011 Safe Food Vol.6 No.1

임신 중기 모체 혈청내 α - FP 과 free β - hCG 농도의 임상적 유용성

정지윤(Ji Youn Chung),김소라(So Ra Kim),원혜성(Hye Sung Won),이필량(Pil Ryang Lee),김암(Ahm Kim) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.7

Objective : The aim of this study was to evaluate the detection rate of the chromosomal abnormalities by screening of maternal serum α-FP, free β-hCG in midtrimester and to evaluate the association of elevated free β-hCG with neonatal & pregnancy outcome. Methods : From October 1995 to December 1999, We studied 8,304 pregnant women who delivered in Asan Medical Center. We measured the levels of serum α-FP and free β-hCG in the midtrimester. We reviewed high Down risk group, high NTD risk group and numerous cases of chromosomal abnormalities, retrospectively. To evaluate the association of elevated free β-hCG with neonatal & pregnancy outcome, we reviewed birth weight, apgar score, gestational age at delivery, presence of PIH or fetal growth restriction. Results : Two hundred ninety eight (3.6%) women were screened as high Down risk pregnancy, 61 (0.7%) women were screened as high NTD risk pregnancy. In the high risk group of chromosomal abnormalities, there were 13 cases of various chromosomal abnormalites, including of six Down syndrome baby. In the low risk group, there were two (0.02%) Down syndrome baby and 8 cases of other chromosomal abnormality. By using maternal serum α-FP, free β-hCG in mid-trimester, 68% of the detection rate of chromosomal abnormalites and 4.1% of false positive rate was reported. The detection rate of Down syndrome is 75% (6/8) and 3.5% of false positive rate, 0.02% of false negative rate were reported. In the aspect of pregnancy outcome, when the free β-hCG more than 2.5MOM, there were more numerous preterm delivery (8.2% vs 3.8%), IUGR (4.6% vs 1.7%), lower 1 min apgar score than 7 (6.4% vs 2.7%). Conclusion Maternal serum α-FP, free β-hCG is at least useful method as Double markers screening method for fetal chromosomal abnormalites and maybe elevated free β-hCG level can be useful marker of poor pregnancy outcome such as PIH, low birth weight or preterm delivery.

질출혈을 동반한 자궁내 초기임신에서 혈중 CA125의 임신예후 예측 효용성

정지윤 ( Ji Youn Chung ),문종수 ( Chong Soo Moon ) 대한산부인과학회 2010 Obstetrics & Gynecology Science Vol.53 No.6

Objective: To investigate whether sudden rise or already increased level of maternal serum CA125 predicts spontaneous abortion in early pregnancy with vaginal bleeding. And if so, to decide the CA125 cut-off level for poor pregnancy outcomes. Methods: The prospective study was performed in forty women presenting with any vaginal bleeding at early pregnancy. Maternal serum CA125 levels were evaluated on the first visit and followed up during the first trimester, or until the time to diagnose abortion. Serial maternal serum β-hCG and ultrasonographic evaluation were also performed at the same time. Results: There were eventually fourteen abortions and twenty-six ongoing pregnancies. Using receiver-operating characteristic curve, 150 U/mL of serum CA125 was the most useful value for predicting spontaneous abortion. In most of abortion women, the serum CA125 level was increased over than 150 U/mL immediately before the abortion. The twelve of 17 women who were 150 U/mL or more resulted in abortion, while there were just two cases of abortion among 23 women who were less than 150 U/mL. Its positive predictive value was 70.6% and negative predictive value was 91.3% respectively (P<0.01). Conclusion: There was a strong correlation between high level of serum CA125 and subsequent spontaneous abortion in early pregnancy. It should be valuable to check maternal serum CA125 added to β-hCG and ultrasound for predicting pregnancy outcome at the early pregnancy, especially in the cases with vaginal bleeding.

산전 초음파에 의해 진단된 후복막강내 Fetus in Fetu 1 예

정지윤(Ji Youn Chung),김소라(So Ra Kim),원혜성(Hye Sung Won),이필량(Pil Ryang Lee),최재성(Jae Sung Choi),구본상(Bon Sang Koo),이향숙(Hyang Suk Lee),김암(Am Kim) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.7

Fetus in fetu is a very rare pathologic feature consisting of a parasitic twin included within the body of the other twin. Fetus in fetu within about 7×6×5 ㎝ sized cystic mass in the fetal retroperitoneum was detected and diagnosed during antenatal ultrasound and confirmed by computed tomography scan postnatally. The mass was removed after birth. The cyst contain yellowish serous fluid with two solid masses. Two solid masses were confirmed fetus in fetu by gross and microscopic pathologic examination. The larger one showed well-developed and organized internal organ.

Comparative Study on Picryl Chloride (PCL)-induced Contact Dermatitis in Female IQI/Jic and BALB/c Mice

Ji-Youn Jung(정지윤) 한국실험동물학회 2013 한국실험동물학회 학술발표대회 논문집 Vol.2013 No.8

생화학,분자생물학 : 시금치종자의 α- glucosidase에 의한 L-ascorbic acid로부터 ascorbic acid-2-glucoside의 생산

정지윤 ( Ji Youn Chung ),송희상 ( Hee Sang Song ),방원기 ( Won Gi Bang ) 한국응용생명화학회(구 한국농화학회) 2004 Applied Biological Chemistry (Appl Biol Chem) Vol.47 No.2