버섯으로부터 새로운 암전이 억제물질 개발을 위한 혈관신생 억제물질의 탐색

정승찬,김재호,박정식,이종수 한국버섯학회 2003 한국버섯학회지 Vol.1 No.1

Clinical Characteristics and Treatment of Cryptorchidism in Adults: A Single Center Experience

정승찬,이승수,구자윤,이상돈 대한남성과학회 2014 The World Journal of Men's Health Vol.32 No.2

Purpose: We evaluated the clinical and therapeutic characteristics of adult cryptorchidism, which have been difficult to establish, given the small number of patients presenting to outpatient clinics.Materials and Methods: A retrospective study of 20 adult patients with cryptorchid testis was performed. We analyzed their palpation of testis, location, surgical method, marital status, number of children, histologic findings, and semen analysis.Results: In 17 cases with unilateral cryptorchidism, the mean age at surgery was 31.1±12.5 years. Fourteen patients had a palpable, small cryptorchid testis. The location of the testis, as verified, was inguinal in 14, prepubic in 2, and intra-abdominal in 1, respectively. We performed orchiopexy in 14 out of 15 patients who sought to have this procedure. Four among 6 married patients had children. Testis biopsy was performed in 12 patients, and all showed abnormal histologic findings. Three among 4 patients, performed semen analysis, showed abnormal findings. In three cases of bilateral cryptorchidism, the mean age at surgery was 35.7±12.5 years. All of these patients had palpated cryptorchid testes in the inguinal area and sought to have a bilateral orchiopexy. Two patients have been married but had no children. All showed abnormal findings in both testis biopsy and semen analysis. No testicular neoplasm was detected during the duration of follow-up.Conclusions: Most adult patients with cryptorchidism preferred orchiopexy to orchiectomy. However, most of patients showed abnormal histology of the testis and semen analysis. Therefore, orchiopexy with regular scrotal examination may be considered a suitable treatment options for adult cryptorchidism.

전통주 제조용 발효제의 특성 및 진균류의 분리

정승찬,유미정,조윤경,이종수,Jeong, Seung-Chan,Yu, Mi-Jung,Cho, Yun-Kyoung,Lee, Jong-Soo 배재대학교 자연과학연구소 2003 自然科學論文集 Vol.13 No.1

고부가가치를 지닌 전통 민속주를 개발하기 위하여 먼저 시중에서 구입한 전통주 발효제의 미생물 분포를 조사한 결과, 세균이 가장 많았고($1.3\times10^7$ CFU/g-Koji), 효모와 곰팡이는 비슷하게 분포하였다. 또한 발효제의 amylase 활성은 120.0 Unit/g 이었고, protease 활성은 36.6 Unit/g 이었다. 전통주 발효제로부터 최종 6균주의 효모들이 분리되었고 Hansenula alni (No 1), Hansenula canadensis (No 2), Hansenula silvicola (No.3), Hansenula califrnica (No 4), Hansenula beijerinckii (No 9), Hansenula saturnus var. sturnus (No11) 등으로 동정되었으며 Saccharomyces 속균은 분리되지 않았다. 전통주 발효제로부터 14균주의 곰팡이를 순수분리하여 동정한 결과 1-41번 균주들은 Rhizopus sp.으로 추정되었고, 나머지 46번, 53번과 64번 균주들은 모두 Aspergillus sp.로 추정되었다. 이 균주들 가운데 Rhizopus sp. 균들은 a-amylase 활성이 없었고 오직 Aspergillus sp. (46번 균주)만이 a-amylase 활성이 5 Unit 이었으며 Rhizopus sp.인 8번 균주의 protease 활성이 45.2 Unit으로 가장 높았다. Microflora and enzyme activity of traditional wine-koji were investigated. Bacteria was contained the greatest of $1.3\times10^7$ CFU/g-Koji, and its amylase and protease activities were 120.0 u/g and 36.6 u/g, respectively. 6 Kinds of yeast were isolated from the koji and identified as Hansenula alni (No 1), Hansenula canadensis (No 2), Hansenula silvicola (No.3), Hansenula califrnica (No 4), Hansenula beijerinckii (No 9) and Hansenula saturnus var. sturnus (No11). Furthermore, 14 kinds of mold were also isolated from the koji and identified as Rhizopus sp(No 1-41, 11 species) and Aspergillus sp.(No. 46, 53, 64, 3 species). Only Aspergillus sp. No 46 was showed a-amylase activity of 5.5 Unit and protease activity of Rhizopus sp. No 8 was the highest of 45.0 Unit.

Characteristics of Patients Who Visited the Emergency Room after Prostate Biopsy: Single Center Retrospective Study

정승찬,이승수,정재민,이상돈 대한요로생식기감염학회 2015 Urogenital Tract Infection Vol.10 No.2

Purpose: To educate patients and prevent biopsy-related complications, it is helpful to understand the causes for visiting the emergency room (ER). Therefore, we want to analyze the causes and factors of complications that cause patients to visit the ER after prostate biopsy.Materials and Methods: We conducted a study of in-patients who visited the ER of Pusan National University Yangsan Hospital after prostate biopsy from December 2008 to July 2015. Age, postoperative interval before visiting the ER, Charlson comorbidity index (CCI) score, symptoms in ER, prostate size, pathologic result, and number of biopsy cores were analyzed retrospectively.Results: Among all 1,694 cases of patients who had undergone prostate biopsies during a 7-year period, only 37 patients (2.2%) visited the ER. Diabetes mellitus (DM) is the most common underlying disease among patients with accompanying infection-related symptoms compared to patients with accompanying non-infection-related symptoms (p<0.001). In univariate analysis, DM (p=0.004) and CCI score (p=0.030) were statistically significant risk factors for infection, but only DM was significant in multivariate analysis (p=0.004). Prostate size (p=0.044) was a significant risk factor for acute urinary retention (AUR) in univariate analysis, but not statistically significant in multivariate analysis. CCI score was a statistically significant risk factor for bleeding (p=0.005 [univariate], 0.035 [multivariate]).Conclusions: AUR after transrectal ultrasound-biopsy is the most common reason for visiting the ER. CCI score showed correlation with bleeding and DM showed correlation with infection. Consideration of risk factors of complications after prostate biopsy will be helpful to the patients in the treatment and prevention of complication.

버섯으로부터 새로운 암전이 억제물질 개발을 위한 혈관신생 억제물질의 탐색

정승찬 외 한국버섯학회 2003 한국버섯학회지 Vol.1 No.1

버섯으로부터 새로운 암전이 억제물질을 개발하기 위하여 먼저 7종 52균주의 자실체 (또는 균사체)에 대한 다양한 추출물들을 제조한 후 이들의 암전이 억제에 관련된 혈관신생 억제활성을 수정란을 이용하여 Choriollantoic membrane(CAM) assay로 조사하였다. 7종 52균주의 버섯에 대한 물, 에탄올, 메탄올 추출물 중에서 장수버섯(Fomitella fraxinea) ASI 17003과 17009의 물 추출물과 비늘버섯(Pholiota sp.) ASI 24008과 잎새버섯(Grifola frondosa) ASI 9017의 에탄올 추출물, 그리고 차가버섯(Inonotus obliquus) ASI 74012의 메탄올 추출물에서 10㎍/egg농도일 때 62.5%~68.8%의 높은 혈관신생 억제활성을 보였다. 그러나 추출 수율과 안전성 등을 고려하여 비늘버섯 ASI 24008을 혈관신생 억제효과 우수버섯으로 최종 선정하여 현재 이들의 암전이 억제 기작 규명을 위한 정제 실험을 실시하고 있다. This study was carried out to obtain new cancer metastasis inhibitor from mushrooms. Extracts from 52 isolates belong to 7 species of mushrooms were prepared by water, ethanol and methanol extractions and its antiangiogenic activity were investigated by choriollantoic membrane(CAM) assay. Water extracts of Fomitella fraxinea ASI 17003 and ASI 17009 fruiting bodies, ethanol extract of Pholiota sp. ASI 24008 and Grifola frondosa ASI 9017 fruiting bodies and methanol extract of Inonotus obliquus ASI 74012 mycelia had the potential anti-angiogenic activity of 62.5%~68.8%. Finally, Pholiota sp. ASI 24008 was selected as a producer of cancer metastasis inhibitor on the basis of their solid yield and anti-angiogenic activity, etc.

내염성 효모의 분리 및 세포외 Protease의 생산

정승찬(Seung-Chan Jeong),현광욱(Kwang-Wook Hyun),김재호(Jae-Ho Kim),이종수(Jong-Soo Lee) 한국생물공학회 2001 KSBB Journal Vol.16 No.2

Secondary surgery for vesicoureteral reflux after failed endoscopic injection: Comparison to primary surgery

이승수,정승찬,정재민,이상돈 대한비뇨의학회 2016 Investigative and Clinical Urology Vol.57 No.1

Purpose: As endoscopic treatment for vesicoureteral reflux (VUR) has increased, secondary ureteral reimplantation (UR) after failure of endoscopic treatment has increased. We studied the clinical feature and efficacy of secondary UR after failure of endoscopic treatment compared with primary UR. Materials and Methods: Eighty-one children who had UR for VUR were enrolled. Charts were reviewed retrospectively for age, sex, grade of VUR before surgery, operative time, hospitalization period, postoperative complication, and success rate. Primary UR (group A, n=64) was compared with secondary UR after failed endoscopic treatment (group B, n=17). In group B, telephone survey for the satisfaction of endoscopic treatment and surgery was done. Results: Mean age of each group was 49.6±37.1 and 56.6±22.5 months (p=0.236). There was no significant difference between each group in sex, mean operative time, postoperative transfusion, complication rate, and success rate. As telephone survey in group B, eleven responders preferred endoscopic treatment as primary treatment of VUR because it was a simple method and no hospitalization. Conclusions: Secondary UR after failure of endoscopic treatment was similar to primary UR. Parents preferred endoscopic treatment as first line treatment for VUR in spite of the need for secondary UR after failure of endoscopic treatment.

Characterization of Antihypertensive Angiotensin I-Converting Enzyme Inhibitor from Recombinant E. coli

Kim, Jae-Ho,Jeong, Seung-Chan,Lee, Dae-Hyoung,Lee, Jong-Soo 배재대학교 자연과학연구소 2005 自然科學論文集 Vol.16 No.1

안지오텐신(ACE) 저해제는 항고혈 효과를 갖고 있으므로 오랫동안 고혈압의 예방이나 치료에 이용되어 왔다. 본 연구는 재조합 대장균으로부터 새로운 ACE 저해제를 생산하고 정제하며 나아가 이들이 구조-기능 관P를 규명하기 위해 수행되었다. Saccharomyces cerevisiase의 ACE 저해 펩타이드 유전자를 함유하고 있는 재조합 pGEX-4T-3을 대장균 BL21(DE3)로 형질전환 시켰다. 재조합 pGEX-4T-3을 갖고 있는 대장균 BL21(DE3)로부터 생산된 Glutathione-s 전이효소 (GST) 융합 단백질을 얻어서 그중 ACE저해 펩타이드를 Sephadex G-25 컬럼 크로마토그래피로 정제하였다. 정제된 ACE 저해 펩타이드는 타이로신-아스파틱엑시드-그리신-글리신-발린-패닐알라린-아르기닌-발린-타이로신-트레오닌의 서열을 가진 새로운 decapeptide이었고 ACE에 대하여 경쟁적으로 저해하였다. The angiotensin I-converting enzyme (ACE) inhibitor has anti-hypertensive effects and has long been used as prevention or remedy of hypertension. This study were carried out to produce and purify a new ACE inhibitor from recombinant E. coli and further elucidate its structure-function relationship. Recombinant pGEX-4T-3 containing ACE inhibitory peptide gene of Saccharomyces cerevisiae was transformed into E. coli BL21(DE3). Glutathione-S transferase (GST) fusion protein from E. coli BL21(DE3) harboring the recombinant pGEX-4T-3 was obtained and the ACE inhibitory peptide was purified with Sephadex G-25 column chromatography. The purified ACE inhibitory peptide was a novel decapeptide with sequence Tyr-Asp-Gly-Gly-Val-Phe -Arg-Val-Tyr-Thr which shows very low similarity to the other ACE inhibitory peptide sequence. The purified ACE inhibitor competitively inhibited ACE.

열성요로감염 환아에서 Top-Down 접근과 Down-Top 접근의 비교분석

안재현,정승찬,이상돈 대한요로생식기감염학회 2013 Urogenital Tract Infection Vol.8 No.1

Purpose: We performed a comparative analysis of the usefulness of top-down and down-top approach in children with febrile urinary tract infection (UTI). Materials and Methods: Among 79 children, 42 children had undergone voiding cystourethrography (VCUG) and dimercaptosuccinic acid (DMSA) scintigraphy. High grade vesicoureteral reflux (VUR) was defined as grade VI and V. We analyzed the detection rate of VUR between down-top (group I) and top-down approach (group II). Results: The mean age of 42 children (Boys 25, girls 17) was 30.3±33.2 years. Among 42 children, 22 (52.4%) were diagnosed as VUR. Detection rate of VUR in groups I and II was 52.4% and 33.3%, respectively (p=0.078). Detection rate of VUR according to sex and age was 56.0% and 36.0% in boys (p=0.156), 47.1% and 29.4% in girls (p=0.290), and 38.9% and 16.7% in children younger than one year of age (p=0.041), and 62.5% and 45.8% in children older than one year of age (p=0.247), respectively. In high grade VUR, there was a similar detection rate of VUR, regardless of sex or age. Sensitivity, specificity, and positive prediction rate of DMSA scintigraphy in all patients and children with high grade VUR were 63.6%, 80.0%, 77.8%, and 84.6%, 80.0%, 73.3%, respectively. Conclusions: In children with febrile UTI who were younger one year of age, down-top approach was more useful for diagnosis of VUR. Therefore, VCUG may initially be needed for diagnosis of VUR in children with febrile UTI who were younger than one year of age.

요로감염 환자에서 소아 및 성인의 원인균주 및 항생제내성의 차이점

남형종,정승찬,이종윤,이상돈 대한요로생식기감염학회 2012 Urogenital Tract Infection Vol.7 No.2

Purpose: Urinary tract infection (UTI) is one of the most common infectious diseases in children and adults. It is widely known that most UTI is caused by E. coli. In most cases for those who underwent UTIs,empirical antibiotics were treatment of choice. But resistance of empirical antibiotics is increasing gradually. The proper use of antibiotics is essential in the clinical field. We evaluated the antibiotic resistance of organisms causing UTIs in children and adults patients to provide the proper use of empirical antibiotics. Materials and Methods: From January 2011 to December 2011, 749 cases (mean age was 27.9±30.2years) who underwent positive urinary culture tests were evaluated. The age, sex, results of urine dipstick and microscopic tests, urine culture, and antibiotics resistance were reviewed. Results: 460 men and 289 women were evaluated. Adults were 310 cases (41.4%) and children were 439 cases (58.6%). Pathogens from the results of urine culture were E. coli (35.7%), Enterococcus species (25.8%), Klepsiella (12.9%). Antibiotics resistance rate of all pathogens was 29.8% and Pseudomonas (36.1%), with E. coli (32.8%) having the highest resistance rate of all. Ampicillin (61.8%), trimethoprim/sulfamethoxazole (42.6%), and penicillin (39.4%) showed higher resistance rates than other antibiotics. The multi-drug resistance rate was 17.8% in total, adults were 17.4%, and children were 18.1%. Female urine RBC and urine WBC counts were associated with UTIs from a pathogen which has resistance at to at least one of the antibiotics in adults. On the other hand, older age, urine leukocyte esterase and urine RBC count was associated with antibiotics resistance in children. Conclusions: Gram positive microorganisms including E. coli were the most common pathogen of UTIs both in adults and children. Therefore, quinolones and cephalosporins are widely used in UTIs, however antibiotic sensitivity was reduced in this study and there was no difference in adults and children. The sensitivity and drug resistance were changing steadily, affecting many factors and various pathogens gave rise to UTIs. Therefore considerations of many factors of sensitivity to antibiotics are needed.