Methylophaga aminosulfidovorans SKI bfmo 오페론의 클로닝 및 구조 분석

임현숙,구재환,김리현,김시욱,조은희,Lim Hyun Sook,Goo Jae Whan,Kim Lee Hyun,Kim Si Wouk,Cho Eun Hee 한국미생물학회 2005 미생물학회지 Vol.41 No.1

Methylophaga aminosulfidovorans SK1 (KCTC 10323 BP)은 단일 탄소원, 질소원 그리고 에너지원으로 난분해성 화합물인 트리메틸아민을 이용할 수 있다. M. aminosulfidovorans SK1는 진핵세포의 flavin-containing monooxygenase와 유사한 유전자(bFMO)를 지니고 있으며 대장균에서 발현된 재조합 단백질은 강력한 트리메틸아민 산화활성을 보인다. 본 연구에서는 bEMO의 기능과 조절 메커니즘을 연구하기 위하여 bfmo의 상단부 및 하단부 유전자의 염기서열을 결정하였다. bfmo 상단부의 세 개의 열린해독틀은 잘 보존된 nitrate/nitrite response regulators와 methyl accepting protein 유사단백질을 암호화하였다. 하단부의 두 개의 작은 열린해독틀은 기능은 알려져 있지 않지만 진정세균계에서 잘 보존된 단백질의 일종으로 나타났다. 역전사효소 중합효소증폭반응을 통하여 여섯 개의 유전자는 세 개의 독립된 오페론으로 구성되어 있음을 확인하였다. bfmo의 상단부에 위치하는 세 개의 조절유전자는 두 개의 프로모터에서 전사되었다. 그리고 이와 독립적으로 bfmo와 두 개의 하단부 유전자가 하나의 전사단위를 이루고 있다. Methylophaga aminosulfidovorans SK1 (KCTC 10323 BP) can utilize trimethylamine as a sole carbon, nitrogen, and energy source. The bacterial flavin-containing monooxygenase (bFMO) gene was identified in the strain and the recombinant enzyme expressed in E. coli oxidized trimethylamine. To study the function and regulation of the bfmo, over 8,000 nucleotide sequences of the neighboring regions including the bfmo were determined. Three open reading frames proceeding to the bfmo gene encoded analogues to highly conserved nitrate/nitrite sensing two-component system regulators and a methyl accepting protein. Two small open reading frames just downstream of the bfmo gene showed no similar proteins of known functions but the sequences were conserved among other bacteria. Reverse transcription-polymerase chain reaction analysis showed that the six putative genes consisted of three transcription units. The three regulatory genes located upstream of the bfmo gene formed two separate transcription units. The bfmo and the two downstream genes were transcribed from a single promoter.

Comparison of Radiographic Measurement and Clinical Parameters of Periodontal Condition on Premolars and Molars

임현숙,최성호,조규성,채중규,김종관,Lim, Hyun-Sook,Choi, Seong-Ho,Cho, Kyoo-Sung,Chai, Jung-Kiu,Kim, Chong-Kwan The Korean Academy of Periodontoloy 1998 Journal of Periodontal & Implant Science Vol.28 No.1

치주상태의 진단은 일반적으로 치주탐침과 같은 임상적 검사와 보조적으로 방사선 검사를 이용한다. 방사선학적인 검사가 치주조직의 파괴정도와 골 흡수를 진단하는데 유용하지만 실제적인 골 흡수를 정확하게 나타나는데는 한계가 있다고 하였다. 그리고 임상적 측정과 방사선 사진을 비교연구하여 이들간의 관계를 알아보고자 하는 노력이 있었다. 방사선이 임상에서 참고사항으로는 중요하지만 절대적 신뢰를 주는데는 문제가 있는 것으로 생각되어왔다. 방사선학적 측정과 임상지수 측정간의 상관관계에대하여 계속적인 연구를 통해 규명하는 것이 중요하다고 생각되어 저자는 연세대학교 치과 대학병원 치주과를 내원한환자중 연령 및 성별에 관계없이 전신질환이 없는 환자 130명을 대상으로 소구치의 원.근심부, 대구치의 원.중.근심부에서 치주낭의 깊이와 (P.D), 부착상실 (A.L)을 측정하였다. 방사선 사진상의 원.근심부에서 백악 법랑질 경계부에서 치조골능 상부까지의 치조골 수준 (Rx.B.L)을 측정하였고 이개부 부위에서는 이개부의 천정으로부터 나타나는 골 흡수의 방사선 투과상을 측정하였다. 이를 기초로 임상지수와 방사선학적 측정간에 상관관계를 연구하여 다음과 같은 결론을 얻었다. 1. 원·근심부의 P.D와 Rx.B.L을 비교 했을 때 상관관계는 모든 치아에서 높게 나타났다(r=0.897$\sim$r=0.725, p<0.01). 2. 원·근심부의 A.L와 Rx.B.L을 비교 했을 때 상관관계는 모든 치아에서 높게 나타났다 (r=0.915$\sim$r=0.732, p<0.01 ). 3. 대구치 769개가 실험 대상인었다. 상악 대구치중(356개) 40%가 이개부 병소를 나타냈고 (146개), 하악 대구치중(413개) 41%가 이개부 병소를 나타냈다(170개). 4. 대구치의 이개부 병소의 중앙부에서 측정한 P.D와 Rx.B.L을 비교 하였을 때 상관관계는 상악 제1대구치를 제외한 모든 치아에서 높게 나타 났다(r=0.811$\sim$r=0.435, p<0.01). 5. 대구치의 이개부 병소의 중앙부에서 측정한 A.L와 Rx.B.L을 비교 하였을 때 상관관계는 상악 제1대구치를 제외한 모든 치아에서 높게 나타 났다(r=0.906$\sim$r=0.479, p<0.01). 이상의 결과를 종합하여 볼 때 임상지수와 방사선학적 측정을 비교한 바, 이들의 상관관계는 모든 치아에서 밀접하게 나타났다. 따라서 방사선학적 측정은 임상지수와 더불어 치주질환의 진단과 치료계획에 크게 기여할 수 있을 것으로 사료된다.

영양교육이 당뇨병환자의 식사요법에 대한 지식과 실천 및 인지도, 혈당관리에 미치는 영향

임현숙(Lim Hyun Sook),천종희(Chyun Jong Hee),김용성(Kim Yong Seong),남문석(Nam Moon Suk) 韓國營養學會 2001 Journal of Nutrition and Health Vol.34 No.1

연구논문 : 감과피 분말을 첨가한 쿠키의 품질특성

임현숙 ( Hyun Sook Lim ),차경희 ( Gyung Hee Cha ) 한국식품조리과학회(구 한국조리과학회) 2014 한국식품조리과학회지 Vol.30 No.5

Traditionally, the persimmon Gojongsi (Diospyros kaki Thunb) is peeled to make dried persimmons and the skins are thrown away. In this study, the quality characteristics of cookies containing persimmon peel powder were tested for recycling of the persimmon peels. The amounts of persimmon peel powder added to the cookies were about 0, 5, 7, 9 and 11%. The densities of the cookies of the control group and the persimmon peel powder containing experimental group were 1.25 and 1.25∼1.37 respectively. The pH was 6.02 for the control group and 5.95~6.01 for the experimental group. Significant differences in the moisture content were observed between groups at 3.34 and 2.16~3.31 for the control and experimental groups, respectively(p<0.05). The spreadabilities and loss rates of the cookies increased with increasing amounts of persimmon peel powder(p<0.05). In contrast, the loss rates and leavening rate of the cookies decreased significantly with decreasing amounts of persimmon peel powder(p<0.05). The lightness of the cookies showed significant decrease(p<0.05), while the redness and yellowness increased with increasing amounts of persimmon peel powder. The hardness(kg) of the control group was 0.847 while that of the experimental group was 0.904~1.110 In the QDA, the results of sensory characteristic analysis showed that the experimental group earned 11% higher scores for color, flavor, taste, and bitterness, 7% higher for appearance and 9% higher for after taste. the consumer acceptance test revealed the experimental group to have a 7% more positive evaluation for color, favor, taste and texture than the control group. On the grounds of the experiment above, the optimal mixture ratio from the sensory test was found to be 7% persimmon peel powder in the cookies.

가지이 모토지로 (梶井基次郞) 연구 - 『 레몬 ( 영몽 ) 』을 중심으로 -

임현숙(Hyun Sook Lim) 한국일본어문학회 1998 日本語文學 Vol.5 No.-

N/A N/A

국내 요양병원 간호사의 직무 역할

임현숙(Hyun Sook Lim),정향인(Hyang In Chung) 한국건강간호융합학회 2024 한국건강간호융합학회지 Vol.1 No.1

Purpose : The purpose of this qualitative study was to identify the detailed job roles of nurses working in long term care hospitals in Korea. Methods : Data were collected using individual in-depth interviews and analysed with a content analysis method. Participants were 11 nurses working more than one year in long term care hospitals. Results : A total of 4 categories, 33 subcategories, and 105 codes were generated from 465 meaningful statements (job roles). Then, 33 subcategories were classified into 4 categories (nursing roles, nursing roles/physician’s roles, physician’s roles and nursing staff roles). Among 105 of the long-term care hospital nurse job roles, thirty-eight(36.2%) was nursing role, eleven(10.5%) were nursing or physician’s roles, twenty- five(23.8%) were physician’s roles and thirty-one(29.5%) were nursing staff roles. In addition to pure nursing work, other roles also showed an high rate(63.8%). Conclusion : Guidelines for the job roles of nurses in long-term care hospitals, as well as appropriate qualifications and compensation standards, must be established.

도축우의 장내용물에서 Listeria속균의 분포도 조사

임현숙 ( Hyun Sook Lim ),서동균 ( Dong Kyun Suh ) 한국동물위생학회 2005 한국동물위생학회지 (KOJVS) Vol.28 No.2

We surveyed the distribution of Listeria spp in intestinal contents of slaughtered cattle from Daegu between March and October 2003. Fourteen Listeria spp were isolated from a total of 100 samples. Two samples contained only L innocua and other six samples contained both L monocytogenes and L innocua. Of the 99 samples positive to esculin reaction in Fraser broth, Listeria spp were isolated only from 8% of the samples. Three selective plating medium were examined for detection of Listeria species including Enhanced hemolysis agar, Oxford agar and Palcam agar. It was found that Enhanced hemolysis agar was more effective than Oxford agar and Palcam agar, and that L monocytogenes needed 48 hour growth to give positive reaction.

대구지역 도축장 출하가축의 도체에서 분리한 Staphylococcus aureus의 항생제 내성과 MRSA 검출에 관한 연구

임현숙 ( Hyun-sook Lim ),서동균 ( Dong-keun Suh ),김환득 ( Hwan-deuk Kim ),이혜화 ( Hye-hwa Lee ),김정미 ( Jeong-mi Kim ),임미하 ( Miha Im ),조재근 ( Jae-keun Cho ) 한국동물위생학회 2024 한국동물위생학회지 (KOJVS) Vol.47 No.1

At the present study, it was aimed to explore the states of antimicrobial resistant Staphylococcus aureus isolates from 1,360 chickens, pigs and cattle carcass (400 chickens, 480 pigs and 480 cattle) in Daegu province from January 2022 to December 2022. Among 1,360 samples, 81 of S. aureus were isolated cattle (1.4%), pigs (7.7%) and chickens (9.2%). In antimicrobial susceptibility test, all of the isolates were demonstrated susceptibility to rifampin. But the isolates were showed resistance other antibiotics in order of tetracycline (62.9%), ciprofloxacin (62.9%), tobramycin (58.0%), gentamicin (51.8%), amikacin (40.7%), penicillin (39.5%), clindamycin (35.8%), enrofloxacin (33.3%), trimethoprim/sulfamethoxazole (30.8%), oxacillin (30.8%), minocycline (29.6%), erythromycin (25.9%), quinupristin/dalfopristin (20.9%), chloramphenicol (12.3%), cefoxitin (9.8%). Among the 81 S. aureus isolates, 25 (30.8%) methicillin-resistant staphylococcus aureus (MRSA) were observed. Seven (28.0%) of 25 MRSA harbored mecA gene. About 96% of MRSA were multidrug resistance to at least 3 more drugs. A continuous monitoring and surveillance program to prevent antimicrobial resistance in livestock products is demanded.

마황 또는 에페드린 성분의 비만치료 적용의 안전성 검토

임현숙(Hyun-Sook Lim),부유경(Yoo-Kyung Boo),송시현(Si-Hyun Song) 한국의료정보교육협회 2024 보건의료생명과학논문지 Vol.12 No.1

마황 및 에페드린은 최근 기존의 기능 외에 비만 치료의 목적으로 많이 사용되고 있으나, 지속적으로 안전성에 대한 논란이 제기되어 비만 목적으로 사용하는데 주의를 필요로 한다. 따라서 본 연구는 체중감량을 목적으로 마황 또는 에페드린을 활용한 연구 결과의 타당성을 평가하였다. 또한 비만 치료에 사용될 때 발생할 수 있는 부작용 사례를 검토하였으며, 이에 대한 안전성을 평가하였다. 이를 위해 본 연구에서는 비만 한의임상진료지침에 대해 학술적으로 검토하였으며, 마황 부작용에 대한 사례를 연구하였다. 연구 결과, 비만 관련 마황 사용에 대한 지침 마련에 기여할 수 있을 것으로 기대된다. Ephedra and ephedrine have recently been widely used for obesity treatment purposes in addition to their existing functions, but safety controversy has been continuously raised and caution is required when using them for obesity purposes. Therefore, this study evaluated the validity of the research results using ephedra and ephedrine for the purpose of weight loss. Cases of side effects that may occur when used to treat obesity were reviewed, and their safety was evaluated. To the end, in this study, the obesity korean medicine clinical practice guideline was reviewed academically, and cases of side effects of ephedra was studied. As a result of the study, it is excepted to contribute to the preparation of guideline for the use of obesity-related ephedra.

대구지역 개의 심장사상충 감염실태

임현숙 ( Hyun Sook Lim ),조유정 ( Yoo Joung Cho ),서동균 ( Dong Kyun Suh ),송동준 ( Dong Jun Song ),이춘식 ( Chun Sik Lee ),배영찬 ( Young Chan Bae ) 한국동물위생학회 2003 한국동물위생학회지 (KOJVS) Vol.26 No.2

A survey of canine heartworm(Dirofilaria immitis) infections among dogs in Daegu area was performed from February 2000 to October 2002 using a microfilarial test and an antigen test(AGENtm Canine heartworm). The infection rate of 220 dogs(96 males, 124 females) was 23.2%(51/220) by the microfilarial test, but was 23.6%(52/220) by the antigen test, revealing that 1 of 52 antigen-positive dogs were microfilaria-negative in the peripheral blood. All dogs that were microfilaria-positive were also antigen-positive. The infection rates of heartworm in dogs at the age of <1, 1-3, 4-6, 7-11 and 12-15 years were 2.7%, 17.4%, 41.0%, 42.9% and 57.1%, respectively. Based on the fact that the antigen test is more accurate than the microfilaria test. The infection rates of heartworm in dogs by housing of indoor and outdoor were 6.9% and 46.4%, respectively. Based on the fact that outdoor housing is more infectious than indoor housing.