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임채신,조정래 한국원예학회 2009 원예과학기술지 Vol.27 No.2
The study was conducted to compare susceptibility of pepper fruits to chilling injury during storage. Sweet ‘Cupra’ and hot ‘Nockgwang’ pepper harvested at the mature-green stage were stored at 1, 5 and 10℃. After a 14-day storage, both cultivars showed surface pitting (SP) symptoms at 1 and 5℃ but not at 10℃. Storing fruits at lower temperature caused a marked increase in SP of both cultivars, but more in ‘Nockgwang’ than in ‘Cupra’. ‘Cupra’ exhibited lower respiration and produced higher ethylene than ‘Nockgwang’ during storage regardless of temperatures. Lower temperature induced higher weight loss (WL) and firmness loss (FL) in both cultivars. Compared with ‘Cupra’, about 4-fold higher WL occurred in ‘Nockgwang’ after a 21-day storage regardless of temperature. During storage, electrolyte leakage (EL) of both cultivars increased at 1 and 5℃, whereas it increased relatively little at 10℃. The SP development at 1 and 5℃ appeared to be associated with higher WL, FL, and EL.
채종 시기 및 GA3 처리가 적수크령 종자의 발아율 향상에 미치는 영향
임채신,오주열,석용철,김도한 경상대학교 농업생명과학연구원 2010 농업생명과학연구 Vol.44 No.6
본 실험은 하천과 벽사면의 녹화에 이용되는 벼과 식물 중 적수크령을 대상으로 채종시기와 식물생장조절물질인 GA3 처리가 발아율 향상에 미치는 영향을 조사하여 적정 채종시기와 GA3 농도 및 침지시간을 구명하고자 수행되었다. 채종 기간은 2008년 10월 2일부터 동년 11월 25일까지 2주 간격으로 5회 채종하였다. 채종된 종자의 저장 및 관리는 수확 후 채종된 순서대로 5±1℃의 항온기에 밀폐하여 보관하였다. 저장된 종자의 후숙은 41±3℃ (R.H.=70%) 항온기에서 96시간 처리하였다. 수크령 종자의 발아율, 평균발아일수 및 GA3 처리에 따른 결과는 침지시간에 따른 효과가 나타났고 채종시기는 10월 중순에서 11월 중순경에 채종되는 수크령 종자가 발아율에 더 효과적이라 판단되었으며, 본 실험을 통해 수크령 종자의 발아성을 향상은 물론 녹화용에 이용되는 수크령의 원예적 이용가치를 높이는데 도움이 될 것으로 보여진다. This experiment was conducted to enhance horticultural utilization value of wild graminaceous species on rooftop gardening purpose. Seeds of Pennisetum were harvested 5 times at an interval of 2 weeks from 2 October to 25 November in 2008. Seeds were soaked into solution of GA3 different concentrations and incubated at the condition of 20℃ and 25℃ each for 12 hrs. Germination rate of the after-ripening seeds was above 80% in the 17 and 31 October and 13 November harvest, but germination rate of seeds harvested in the 2 October and 25 November was below 70%. Fresh seed germination and early growth of shoot and root after germination were promoted by GA3 treatment. Effect of GA3 treatments on germinability of fresh seeds was more prominent in the seeds harvested on 2 October and 25 November.
Rebound Inflammation Associated with Rewarming from Hypothermia in an Endotoxin-Injured Lung
임채만 대한중환자의학회 2013 Acute and Critical Care Vol.28 No.2
Background: Hypothermia is known to suppress inflammation in various experimental and clinical settings. We wanted to investigate how the suppressed inflammation by hypothermia is affected during rewarming. Methods: Mice were being assigned to normothermia (37oC) or hypothermia (32oC). After 30 minutes at the assigned temperature, lipopolysaccharide was administered intratracheally. The mice were then randomly grouped and subjected to 4 hours of normothermia (N), 24 hours of normothermia (NN), 4 hours of hypothermia (H), or 4 hours of hypothermia followed by normothermia for the next 20 hours (HN). In another experiment, other HN mice were treated with varying doses of anti-TNF-α or anti-IL-1β antibodies (0, 6.25, 12.5, 25, and 50 μg/250 μl) immediately prior to rewarming. Results: The neutrophil counts of BAL fluid (×104/ml) were 23.0 ± 13.1 in the N, 6.4 ± 3.1 in the H (p = 0.002 vs N), 20.4 ± 10.2 in the NN, and 49.7 ± 21.0 in the HN (p = 0.005 vs H; p < 0.001 vs NN). Myeloperoxidase activity of the lung (unit/μg) was 6.7 ± 2.9, 7.9 ± 1.9, 17.8 ± 4.0 (p < 0.001 vs N), and 12.9 ± 5.9 (p = 0.034 vs H, p = 0.028 vs NN), respectively. Compared with control HN, total WBC and neutrophil counts of mice treated with anti-TNF-α antibody or anti-IL-1β antibody prior to rewarming were lower at all tested doses. The combination of both anti-TNF-α or anti-IL-1β antibodies was not increasingly reducing the neutrophilic sequestration. Conclusions: Rewarming from induced hypothermia resulted in augmentation of neutrophilic sequestration of endotoxin-injured lung. Treatment with antibodies against TNF-α or IL-1β prevented this rebound of neutrophilic infiltration.
패혈증환자에서 APACHE III Scoring System의 예후적 가치
임채만,이재균,이성순,고윤석,김우성,김동순,김원동,박평환,최종무,Lim, Chae-Man,Lee, Jae-Kyun,Lee, Sung-Soon,Koh, Youn-Suck,Kim, Woo-Sung,Kim, Dong-Soon,Kim, Won-Dong,Park, Pyung-Hwan,Choi, Jong-Moo 대한결핵및호흡기학회 1995 Tuberculosis and Respiratory Diseases Vol.42 No.6
연구배경: 중환자의 예후를 정량화하는 체점체계 중 APACHE III system은 중환자실 제 1병일 점수는 물론 일갱신 점수도 환자의 위험도를 갱신하는 것이 알려진 바 있어 패혈증 환자들에서 APACHE III score의 예후 지표로서의 가치를 알아 보고자 하였다. 방법: 48명의 패혈증 환자들에서 후향적으로 중환자실 제 1병일, 2병일 및 3병일의 APACHE III score를 조사하여 패혈증 생존자와 비생존자 간의 차이를 분석하였다. 결과: 패혈증 생존자는 비생존자에 비해 APACHE III system에 따른 중환자실 제 1병일 정수가 유의하게 낮았으며, 제 2일 및 제 3일의 점수도 각각의 전날 점수에 비해 유의하게 감소하였다. 폐혈증 비생존자의 경우 제 1병일 점수가 생존자에 비해 높았고, 제 2병일 및 제 3병일 점수 역시 각각의 전날 점수에 비해 증가하는 경향을 보였다. 결론: APACHE III system에 따른 제 1병일 점수 및 제 3병일까지의 점수의 일변동은 패혈증 환자의 예후를 반영하는 것으로 사려된다. Background: The index which could predict the prognosis of critically ill patients is needed to find out high risk patients and to individualize their treatment. The APACHE III scoring system was established in 1991, but there has been only a few studies concerning its prognostic value. We wanted to know whether the APACHE III scores have prognostic value in discriminating survivors from nonsurvivors in sepsis. Methods: In 48 patients meeting the Bones criteria for sepsis, we retrospectively surveyed the day 1(D1), day 2(D2) and day 3(D3) scores of patients who were admitted to intensive care unit. The scores of the sepsis survivors and nonsurvivors were compared in respect to the D1 score, and also in respect to the changes of the updated D2 and D3 scores. Results: 1) Of the 48 sepsis patients, 21(43.5%) survived and 27(56.5%) died. The nonsurvivors were older($62.7{\pm}12.6$ vs $51.1{\pm}18.1$ yrs), presented with lower mean arterial pressure($56.9{\pm}26.2$ vs $67.7{\pm}14.2\;mmHg$) and showed greater number of multisystem organ failure($1.2{\pm}0.8$ vs $0.2{\pm}0.4$) than the survivors(p<0.05, respectively). There were no significant differences in sex and initial body temperature between the two groups. 2) The D1 score was lower in the survivors (n=21) than in the nonsurvivors ($44.1{\pm}14.6$, $78.5{\pm}18.6$, p=0.0001). The D2 and D3 scores significantly decreased in the survivors (D1 vs D2, $44.1{\pm}14.6$ : $37.9{\pm}15.0$, p=0.035; D2 vs D3, $37.9{\pm}15.0$ : $30.1{\pm}9.3$, p=0.0001) but showed a tendency to increase in the nonsurvivors (D1 vs D2 (n=21), $78.5{\pm}18.6$ : $81.3{\pm}23.0$, p=0.1337; D2 vs D3 (n=11), $68.2{\pm}19.3$ : $75.3{\pm}18.8$, p=0.0078). 3) The D1 scores of 12 survivors and 6 nonsurvivors were in the same range of 42~67 (mean D1 score, $53.8{\pm}10.0$ in the survivors, $55.3{\pm}10.3$ in the nonsurvivors). The age, sex, initial body temperature, and mean arterial pressure were not different between the two groups. In this group, however, D2 and D3 was significantly decreased in the survivors(D1 vs D2, $53.3{\pm}10.0$ : $43.6{\pm}16.4$, p=0.0278; D2 vs D3, $43.6{\pm}16.4$ : $31.2{\pm}10.3$, p=0.0005), but showed a tendency to increase in the nonsurvivors(D1 vs D2 (n=6), $55.3{\pm}10.3:66.7{\pm}13.9$, p=0.1562; D2 vs D3 (n=4), $64.0{\pm}16.4:74.3{\pm}18.6$, p=0.1250). Among the individual items of the first day APACHE III score, only the score of respiratory rate was capable of discriminating the nonsurvivors from the survivors ($5.5{\pm}2.9$ vs $1.9{\pm}3.7$, p=0.046) in this group. Conclusion: In sepsis, nonsurvivors had higher first day APACHE III score and their updated scores on the following days failed to decline but showed a tendency to increase. Survivors, on the other hand, had lower first day score and showed decline in the updated APACHE scores. These results suggest that the first day and daily updated APACHE III scores are useful in predicting the outcome and assessing the response to management in patients with sepsis.