희렴 추출물의 Heme Oxygenase-1 발현을 통한 생쥐 해마 유래 HT22 세포 보호효과

임남경,이동성,김윤철,여선정,정길생,장원영,김기영,고영상,김영리,현진원,강희경,맹영희,현창림 한국생약학회 2012 생약학회지 Vol.43 No.4

Siegesbeckia Herba is known to have anti-oxidant, anti-inflammatory, anti-allergic and anti-tumor. The objective of this study is to explore the neuroprotective effect of Siegesbeckia Herba against glutamate-induced oxidative stress in mouse hippocampal HT22 cells. Siegesbeckia Herba 70% ethanol extract and solvent fractions have the potent neroprotective effects on glutamate-induced nerotoxicity by induced the expression of heme oxygenase (HO)-1 in the mouse hippocampal HT22 cells. Especially, ethyl acetate fraction showed higher protective effect. In HT22 cell, Siegesbeckia Herba ethyl acetate fraction makes the nuclear accumulation of Nrf2. Further, we found that treatment with c-JUN N-terminal kinase (JNK) inhibitor (SP600125)reduced Siegesbeckia Herba ethyl acetate fraction induced HO-1 expression and Siegesbeckia Herba ethyl acetate fraction also increased JNK phosphorylation. In conclusion, the ethyl acetate fraction of 70% ethanol extract of Siegesbeckia Herba significantly protect glutamate-induced oxidative damage by induction of HO-1 via Nrf2 and JNK pathway in mouse hippocampal HT22. Taken together these finding suggest that Siegesbeckia Herba ethyl acetate fraction good source for taking active compounds and may be a potential therapeutic for brain disorder by targeting the oxidative stress of neuronal cell.

Delphinidin Suppresses PMA-Induced MMP-9 Expression by Blocking the NF-κB Activation Through MAPK Signaling Pathways in MCF-7 Human Breast Carcinoma Cells

임남경,장원준,정철호,정길생 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.8

Matrix metalloproteinase-9 (MMP-9) plays an important role in the invasion and metastasis of cancer cells. The synthesis and secretion of MMP-9 can be stimulated by a variety of stimuli, including cytokines and phorbol 12-myristate 13-acetate (PMA), during various pathological processes, such as tumor invasion, atherosclerosis, inflammation, and rheumatoid arthritis, whereas MMP-2 is usually expressed constitutively. Delphinidin, an anthocyanidin present in pigmented fruits and vegetables, possesses potent antioxidant, anti-inflammatory, and antiangiogenic properties. In this study, we investigated the antiproliferative and antiinvasive effects of delphinidin on PMA-induced MMP-9 expression in MCF-7 human breast carcinoma cells using zymography, western blotting, reverse transcription–polymerase chain reaction, and Matrigel invasion assay. Delphinidin significantly suppressed PMA-induced MMP-9 protein expression in MCF-7 human breast carcinoma cells, and it also inhibited the MMP-9 gene transcriptional activity by blocking the activation of NFkappaB (NF-jB) through MAPK signaling pathways. Moreover, the Matrigel invasion assay showed that delphinidin reduces PMA-induced cancer cell invasion. These results suggest that delphinidin is a potential antimetastatic agent that suppresses PMA-induced cancer cell invasion through the specific inhibition of NF-jB-dependent MMP-9 gene expression.

마치현 에틸아세테이트 분획물의 뇌세포 보호효과

임남경,정길생 한국생약학회 2013 생약학회지 Vol.44 No.4

Portulaca oleracea L. is known to have many biological benefits such as anti-oxidant, anti-inflammatory, anti-allergic and anti-tumor. The objective of this study is to explore the neuroprotective effect of P. oleracea L. against glutamateinduced oxidative stress in mouse hippocampal HT22 cells. P. oleracea L. 70% ethanol extract and solvent fractions have the potent neroprotective effects on glutamate-induced nerotoxicity by induced the expression of heme oxygenase (HO)-1 in HT22cells. Especially, ethyl acetate fraction showed higher protective effect. In HT22 cell, P. oleracea L. treatment with ERK inhibitor (PD98059) and c-JUN N-terminal kinase (JNK) inhibitor (SP600125) reduced P. oleracea L. ethyl acetate fraction induced HO-1 expression and P. oleracea L. ethyl acetate fraction also increased ERK and JNK phosphorylation. Furthermore, we found that treatment of P. oleracea L. caused the nuclear accumulation of Nrf2. In conclusion, the ethyl acetate fraction of 70%ethanol extract of P. oleracea L. significantly protect glutamate-induced oxidative damage by induction of HO-1 via Nrf2, ERK and JNK pathway in mouse hippocampal HT22. Taken together these finding suggest that P. oleracea L. ethyl acetate fraction is good source for taking active compounds and may be a potential therapeutic agent for brain disorder that induced by oxidative stress and neuronal damage.

Inhibitory Effect of Fractions from Skullcap (Scutellaria baicalensis Georgi) on Osteoclast Differentiation and Bone Resorption

임남경,이효정,유미희,김현정,이인선 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.5

This study was performed to evaluate the effects of skullcap (Scutellaria baicalensis Georgi, SBG)on differentiation of RAW 264.7 cell into osteoclast-like cells and bone resorption of mature osteoclast to propose candidates for the prevention or treatment of osteoporosis. In the screening of 140 natural products, the selected SBG methanol extract dramatically reduced tartrate-resistant acid phosphatase (TRAP) activity to 21% at 10 μg/mL. The proliferation was evaluated using the MTT assay, and the survival rates of RAW 264.7 cells treated with 10-100μg/mL extract were over 80% with most concentrations. The chloroform fraction from SBG (SBG-C) decreased TRAP activity. The SBG-C increased the expression of the pro-apoptotic protein Bax and reduced the expression of the anti-apoptotic protein Bcl-2. It also stimulated caspase-3activity in osteoclasts. In addition, the actin rings were loose and fuzzy in the presence of these fractions. A resorption pit was formed by multinucleated osteoclastic cells, but was not observed in the presence of the SBG-C (10 μg/mL). In conclusion, we found SBG suppressed osteoclast differentiation and bone resorption. It also inhibited osteoclast survival. Therefore SBG is expected to be a natural source for the development of functional food and medical agents to prevent or treat osteoporosis.

차대차 추돌사고 재현시험을 통한 경추염좌 상해 위험도 연구

임남경(Namkyoung Lim),심상우(Sangwoo Shim),정현철(Hyuncheol Jung) 한국자동차공학회 2016 한국 자동차공학회논문집 Vol.24 No.4

Whiplash injuries in low-speed rear-end collisions are the most common injuries and has been a social issue in insurance industry, such as excessive medical claim costs along with exaggerated injuries of victims and treatments from hospitals. According to the Korea Insurance Development Institute reports, the number of claims by rear-end collision was approximately 703,000, which accounts for 53.6 % of the total car-to-car collisions in 2014. Part of the neck injury claims in the Korea car insurance was approximately 28.3 %. Furthermore, approximately 98.4% of the injured persons in rear-end collisions sustained minor injuries under AIS2. In order to improve this situation as well as find out the severity of neck injuries from rear-end collision, the Korea Automobile Insurance Repair Research and Training Center conducted car-to-car rear-end crash tests that striking vehicles(SUV) collided into different sizes of struck-vehicles(small, middle, and large sedan) at the impact speeds of 8 km/h ~ 16 km/h. In order to analyze the whiplash injury, the BioRID-II was seated in each struck-vehicles, and the neck injury criteria(NIC), head contact time, maximum vehicle accelerations, and mean vehicle accelerations were calculated from values from the accelerations of the dummy and the struck-vehicles.

SUV 추돌사고 형태에 따른 탑승자 경추염좌 상해 위험도 연구

임남경(Namkyoung Lim),심상우(Sangwoo Shim),정현철(Hyuncheol Jung) 한국자동차공학회 2015 한국자동차공학회 학술대회 및 전시회 Vol.2015 No.11

Whiplash injuries in low speed rear-end accidents cause many social problems such as excessive medical claim costs and moral hazards. According to KIDI reports, the number of claims by rear-end collision was about 708 thousand, which account for 52% of total car to car accidents in 2013. And the portion of neck injuries claims in Korea car insurance was about 37.8%. About 99.4% of injured person by rear-end collision accidents had minor injuries under AIS2. As the new car sales of SUV is increasing every year, KART conducted car to car rear-end crash tests with SUV(striking-vehicles) to different size of struck-vehicles(small, middle, large) and impact speed(8~16km/h). And to analyze the whiplash injury, BioRID-II was seated in each struck-vehicles and Neck injury criteria(NIC), head contact time and max & mean vehicle accelerations were calculated from the values like accelerations of Dummy and struck-vehicles.

추돌사고 시 탑승자세 변화에 따른 목상해 위험도 시뮬레이션 연구

임남경(Namkyoung Lim),이재원(Jaewon Lee),임종현(·Jonghyun Yim),심상우(Sangwoo Shim) 한국자동차공학회 2016 한국자동차공학회 학술대회 및 전시회 Vol.2016 No.11

한약재 추출물의 조골세포 분화 및 파골세포 형성에 미치는 영향

임남경(Nam-Kyung Im),김현정(Hyun-Jeong Kim),김미진(Mi-Jin Kim),이은주(Eun-Ju Lee),김혁일(Hyuk-Il Kim),이인선(In-Seon Lee) 한국식품과학회 2010 한국식품과학회지 Vol.42 No.5

천연물 유래의 생약에서 조골세포 증식을 높이면서, 파골세포의 분화 억제에 효과가 있는 시료를 검색하고자 15종의 한약재 추출물의 효과를 검토해 보았다. Mouse calvaria 유래의 osteoblastic cells를 이용하여 세포 생존률 및 ALP 활성을 측정하였으며, 또한 마우스 골수 세포를 이용하여 M-CSF와 RANKL을 처리하여 파골세포의 분화를 유도한 후, 세포 생존율과 TRAP효소활성을 측정하였다. 그 결과, 두충, 곽향, 개다래, 형개, 정공등 추출물은 조골세포 증식 및 ALP 활성를 증가시켰으며, 파골세포 활성을 나타내는 TRAP활성이 억제되는 것으로 확인되었다. 따라서 이 추출물들은 조골세포의 기능을 향상시키는 동시에 파골세포의 기능을 억제하여 골 흡수와 관련된 질환과 함께 골 질환 예방에 효과가 있을 것으로 생각되어진다. Bone is continuously remodeled by osteoblasts and osteoclasts. We investigated the effects of medicinal herbs, which act on bone metabolism. Fifteen kinds of medicinal herb extracts were screened for bone formation activity with osteoblastic cells, and MC3T3-E1 and bone resorption were screened with osteoclasts derived from mouse bone marrow macrophages. Among these samples, Actinidia polygama, Eucommia ulmoides Oliv., Schizonepeta tenuifolia, Sorbus commixta, and Zingiber officinale Rosc. extracts showed strong bone-forming activity accompanied with osteoblast proliferation and alkaline phosphatase activity. In addition, these extracts decreased tartrate-resistant acid phosphatase activity against osteoclast differentiation. The results indicate that these medicinal herb extracts can potentially prevent bone-related diseases such as osteoporosis by increasing osteoblast differentiation and reducing osteoclast activity.

추돌사고 형태에 따른 탑승자 경추염좌 상해 위험도 연구

임남경(Namkyoung Lim),심상우(Sangwoo Shim),이재원(Sangwoo Shim) 한국자동차공학회 2014 한국자동차공학회 학술대회 및 전시회 Vol.2014 No.11

Whiplash injuries in low speed rear-end accidents cause many social problems such as excessive medical claim costs and moral hazards. According to KIDI reports, the number of claims by rear-end collision was about 770 thousand, which account for 48% of total car to car accidents in 2012. And the portion of neck injuries claims in Korea car insurance was about 37.8%. And about 99.4% of injured person by rear-end collision accidents had minor injuries under AIS2. To study the relations among whiplash injury risk, vehicle damage, vehicle size and mass, KART conducted car to car rear-end crash tests with different size of struck-vehicles(small, middle, large) and impact speed(8~16km/h) of striking-vehicles(middle). And to analyze the whiplash injury, BioRID-II was seated in each struck-vehicles and Neck injury criteria(NIC), head contact time and max & mean vehicle accelerations were calculated from the values like accelerations of Dummy and struck-vehicles.

NIDDM 환자에서 경구당부하검사와 공복시 혈청 유리 지방산 농도와의 상관관계

남경제,임경호 인제대학교 1999 仁濟醫學 Vol.20 No.2

Background: NIDDM may have more to do with abnormalities in fat than carbohydrate metabolism. One possible mechanism would be elevation of free fatty acids. This study was conducted to evaluate the correlation between insulin secretory pattern in oral glucose tolerance test and serum free fatty acid concentrations in NIDDM patients. Methods: We performed 75 g oral GTT in 310 NIDDM patients. During oral GTT, we measured serum glucose, insulin, c-peptide, lipid concentrations (total cholesterol, triglycerride, HDL-cholesterol, Lp(a), free fatty acid) Results: 1) There was significant correlation between serum FFA concentrations and body mass index, serum total cholesterol concentrations and triglyceride concentrations. But we cannot find correlation between serum FFA concentrations and ages of patients, duration of disease, HbA1c, serum HDL-cholesterol concentrations and serum Lp(a) concentrations. 2) There was significant correlation between serum FFA concentrations and serum glucose levels on fasting, 30min, 60min. But there was no correlation between serum FFA concentrations and serum insulin concentrations and c-peptide concentrations. 3) There was significant correlation between serum FFA concentrations and serum glucose response area. But there was no correlation between serum FFA concentrations and insulin response area and C -peptide response area. 4) There was no correlation between serum FFA concentrations and fasting glucose/insulin ratio and glucose/C-peptide ratio. Conclusion: These results suggest that there was inadequate interrelation between serum FFA concentrations and parameters of insulin sensitivity derived from oral glucose tolerance test of NIDDM patients.