우유중에 함유되어있는 잔류 항생물질의 검사법

이희무 ( Hee Moo Lee ) 한국균학회 1983 韓國菌學會誌 Vol.11 No.3

새로운 항균활성을 보이는 토양 분리 세균 Paenibacillus polymyxa DY1의 분류와 동정

신은석,이희무,이복권,김성훈,권순일,유관희,Shin, Eun-Seok,Lee, Hee-Moo,Lee, Bok-Kwon,Kim, Sung-Hoon,Kwon, Sun-Il,Yoo, Kwan-Hee 한국미생물학회 2007 미생물학회지 Vol.43 No.1

항생제 내성 세균의 출현으로 새로운 항생물질의 개발에 대한 필요성이 대두되고 있다. 본 연구에서는 새로운 항균활성물질을 개발하고자 강원도 대암산 용늪 토양으로부터 새로운 항균물질을 생산하는 균을 분리하였고, 이를 동정하였다. 생화학적인 시험과 16S ribosomal DNA 염기서열 분석결과 Paenibacillus polymyxa균과 가장 높은 상동성을 보여주었다. 지방산 조성의 분석에서도 이 균주는 Paenibacillus polymyxa와 가장 가까웠다. 이 균주가 생산하는 항균물질은 1군 법정 전염병을 일으키는 Samonella enterica serovar Typhi와 Shigella dysentery, enterohaemorrhagic Eschelichia coli, 그리고 Vibrio cholera등의 병원성 세균에 성장억제 효과를 나타냈으며, 다른 일반 식중독 장내세균에서도 성장억제 효과를 나타냈다. 이 균주가 생산하는 항균활성 물질은 과거에 보고된 것과 다른 새로운 것으로 보이며, 광범위한 항균활성으로 인하여 새로운 항생물질 개발 후보로 많은 잠재력을 가진 것으로 평가된다. The DY1 strain of Gram-positive, rod-shaped bacteria was isolated from the soil sample collected from Daeam mountain, Korea. The culture filtrate of DY1 strain showed a broad spectrum of antimicrobial activity on various pathogenic and food poisoning enteric bacterial species tested in vitro. It showed significant growth-inhibitory effect on Salmonella enterica sp., Shigella sp., pathogenic Escherichia coli, Vibrio cholerae, Vibrio parahemolyticus, and Yersinia enterocolitica. For the identification of the DY1 strain, morphological, biochemical and molecular phylogenetic approaches were performed. The DY1 strain was found to be a member of the genus Paenibacillus on the basis of morphological and biochemical analyses. The 16S rDNA of DY1 showed the highest pairwise identity with Paenibacillus polymyxa with 99.79% (1,413 bp/1,416 bp). The antimicrobial entity from DY1 looked different from preciously reported ones and seems to have a great potential to be further studied as a candidate of new antibiotics to control multi-drug resistant pathogens.

Bacillus thuringiensis serovar kurstaki 돌연변이균주의 편모항원의 변이성

이형환(Hyung - Han Lee),이영주(Young - Chu Lee),이희무(Hee - Moo Lee) 한국미생물생명공학회 1989 한국미생물·생명공학회지 Vol.17 No.1

경북지역 가축에서 분리된 Salmonella typhimurium과 S enteritidis의 phage typing 및 pulsed-field gel electrophoresis

김상윤 ( Sang Yun Kim ),이희무 ( Hee Moo Lee ),김신 ( Sin Kim ),홍현표 ( Hyon Pyo Hong ),권헌일 ( Heon Il Kwon ) 한국동물위생학회 2001 한국동물위생학회지 (KOJVS) Vol.24 No.3

Forty-five Salmonella typhimurium isolates were encountered 8 phage types in which DT197 and U302 were the predominant types. The DT104 type which was first found from pig in Korea, and was resistant to chloramphenicol, streptomycin, sulfamethoxazole/trimethoprim, tetracycline, gentamicin and nalidixic acid. Twenty-two S enteritidis isolates were encountered 5 phage types in which PT4 were the representative (predominant). S enteritidis isolates were susceptible to all antimicrobial agents. As a result of PFGE analysis for S typhimurium and 5 enteritidis, PFGE patterns was better than phage typing in discriminating of strains. PFGE patterns were not in accord with phage type even though some strain had the same phage types.

최근 한국에서 유행하는 장염비브리오균의 분자 역학적 특성

김상숙(Sang-Sook Kim),이희무(Hee-moo Lee),이중복(Jung-Bok Lee) 한국생물공학회 2003 KSBB Journal Vol.18 No.6

경북지역 가축에서 Salmonella속 균 감염증에 대한 역학적 특성

김상윤 ( Sang Yun Kim ),이희무 ( Hee Moo Lee ),김신 ( Sin Kim ),홍현표 ( Hyon Pyo Hong ),권헌일 ( Heon Il Kwon ) 한국동물위생학회 2001 한국동물위생학회지 (KOJVS) Vol.24 No.1

The result of studying the epidemiological characteristics of Salmonella strains which have been isolated from the domestic animals in Gyeongbuk province from February 1998 to August 2000 were summarized as follows. The isolation rates of Salmonella strains were 2.0% from cattle feces, 6.3% from cattle lymph node, 9.5% from pig feces, and 25.1% from pig lymph node. In poultry, the isolation rates were 30.3%. The isolates of Salmonella showed positive reaction for MUCAP test, methyl red test, but showed negative reaction for urea test, indole test, Voges Proskauer test. On TSI agar, the isolates showed acid butt, alkaline slant. Also, the isolates were identified as Salmonella strain by API 20E kit. Non H2S production Salmonella strains isolated from poultry were identified as S gallinarum. As a result of serotyping, B group were the most common in cattle and pig, D1 in chickens. 21 serovars were found. The common serovar from the domestic animals was S typhimurium, S derby, S agona, S schwarzenground, S enteritidis and S gallinarum. The most commonly encountered serovars in cattle were S agona and S typhimurium in pig, S gallinarum in chicken. As a result of antimicrobial susceptibility test, all Salmonella isolates were susceptible to amikacin, ciprofloxacin. Norfloxacin, cefotaxime and polymycin B. The resistance rates to tetracycline and streptomycin was 58% and 56% respectively. 69.3% of all isolates were resistant to more than one antimicrobial agent. Out of the resistant isolates, the isolates resistant to streptomycin and tetracycline was 36%. There were 24 strains of multiresistant isolates resistant to more than 5 antimicrobial agents. S typhimurium were resistant to all antimicrobial agents, also had a lot of multiresistant strains. Therefore, S typhimurium was considered as a major agent of antimicrobial resistance.

경북지역 가축에서 분리된 Salmonella typhimurium과 S enteritidis의 병원성 시험

김상윤 ( Sang Yun Kim ),이희무 ( Hee Moo Lee ),김신 ( Sin Kim ),홍현표 ( Hyon Pyo Hong ),권헌일 ( Heon Il Kwon ) 한국동물위생학회 2001 한국동물위생학회지 (KOJVS) Vol.24 No.1

The result of studying the pathogenicity of Salmonella typhimuriwn and S enteritidis isolated from domestic animals in Gyeongbuk province were summarized as follows. In Congo-red binding test, S typhimurium had much more rough types than S enteritidis. In colicin production test, 4 strains of S typhimurium were positive but all of S enteritidis were negative. In hemolysin production test, all of S typhimurium and S enteritidis were negative. In Guinea pig serum resistant test, all of S typhimurium and S enteritidis were positive. As a result of pathogenicity test to mice, 54.4% of mice were died. Therefore, S typhimurium and S enteritidis were considered as highly pathogenic. S typhimurium DT104 and S enteritidis PT4 were more pathogenic to mice than other phage types of same serovar. S typhimurium and S enteritidis were considered not so pathogenic for 6-day-old chickens. The recovery rates of Salmonella strains from mice and chickens inoculated were 96.8%, and 54%, respectively. In chickens, proportional to the time From 2 weeks after challenge inoculation. The recovery rates were noticeably decreased.

간 및 위 , 장관을 침범한 특발성 과호산구 증후군 치험

정을순(Eul Soon Chung),이상종(Sang Jong Lee),김향(Hyang Kim),김병익(Byung Ik Kim),황기은(Kie Eun Hwang),성기철(Ki Chul Sung),조용균(Yong Kyun Cho),이희무(Hee Moo Lee),금주섭(Joo Seob Keum) 대한소화기학회 1997 대한소화기학회지 Vol.30 No.3

Marked eosinophilia is found in many kinds of disease state, It usually occur in allergic and parasitic conditions and occ assionally in autoimmune disease, blood dyscrasias, or certain malignancics. Idio- pathic hypereosinophilic syndrome is characterized by markedly elevated peripheral blood eosinophil count without explainable etiology. Eosinophilopoiesis is related with T-lymphocyte activation and cytokine cascade controlling eosinophilic production. It shows tissue infiltration in many organs including endomyocardium, lung, liver, gastrointestinal tract. We have recently experienced a case with idiopathic hypereosinophilic syndrome of a 65-year-old woman who had of complained anorexia, weight loss and diarrhea during the past month and half. Persistantly increased eosinophil count was shown in her peripheral blood, the count averaging 3480/mm'(41% in differential count). Abdominal computer tomography disclosed multiple small relatively ill-defined hypoechoic lesions in the liver. Microscopically, the needle biopsy of the liver revealed heavy infiltration by eosinophilis especially in the portal tracts. On reviewing, previously perfomed endoscopic biopsies from the stomach and terminal ileum also had mild to moderate eosinophilic infiltration, respectively. No evidence of parasitic infection was present on the clinical history and laboratory test. Corticosteroid was administered ard brought marked improvement of clinical manifestations including both hematologic and radiologic abnormalities. (Korean J Gastroenterol 1997; 30:397- 403)

유방암 유즙에서 분리한 포도구균의 분자생물학적 typing과 multiplex PCR을 이용한 장독소의 검출

김신 ( Shin Kim ),홍현표 ( Hyon Pyo Hong ),김상윤 ( Sang Yun Kim ),권헌일 ( Heon Il Kwon ),이희무 ( Hee Moo Lee ) 한국동물위생학회 2002 한국동물위생학회지 (KOJVS) Vol.25 No.3

Forty strains of Staphylococcus aureus were isolated from mastitic milk. As a result of antimicrobial susceptibility test, the strains of S aureus revealed 47.5% were resistant to ampicillin and penicillin, and 7.5% to gentamicin. But 45% of isolates were sensitive to antimicrobial agents tested. In case of enterotoxin production, 56.3% of 16 strains produced enterotoxin D. Two strain of enterotoxin D producers produced both enterotoxin B and D. According to isolation date, 15 representative strains were selected. As a results of pulsed field gel eletrophoresis analysis of the 15 representative strains, 14 strains were identical. Therefore we consider the identical strains of S aureus have caused continuously bovine mastitis in this dairy farm. If autogenous vaccine can be made by the strains, it will work well for the prevention of bovine mastitis caused by S aureus.

토양미생물에 의한 Crude oil 분해능 및 활성측정

이희무,권기석,이중복,이장순 안동대학교 환경연구소 2001 환경연구 Vol.1 No.-

유류(油類)를 탄소원으로 하는 토양 미생물의 순수 분리 및 분해 활성에 따른 결과는 다음과 같다. 1. 토양 미생물 중 원유 성분 중에 PAHs 물질을 탄소원으로 이용하는 균주를 혼합 균주로 분리하여 유일 탄소원으로 crude oil 29%가 포함된 평판배와 액체배지에서 단일 균주로 성장을 확인하여 분리된 35번 균주를 동정한 결과 Acinetobacter lwoffii 근연종로 동정되었다. 2. 35번 균주의 원유 분해능은 GC 측정결과를 보면 배양시간이 지날수록 원유 성분이 분해되어 peak가 감소되었으며, crude oil내의 화합물의 분해양상을 보면 탄소수가 많은 물질은 시간이 지남에 따라 서서히 감소하는 경향을 보였다. 탄소수가 적은 화합물질은 배양시간이 지남에 따라 감소하다 증가하는 경우도 생기며, 다시 감소하는 경향을 보이는데, 이는 탄소수가 많은 물질이 분해가 되어 탄소구가 적은 화합물질이나 전 물질의 중간 유도체 물질로 축적되었을 경우로 추정된다. 3. 원유성분 중 탄소수가 적은 화합물질 (저분자 PAHs)을 미생물이 쉽게 분해한다. 따라서 탄소구가 많은 화합물질 (고분자 PAHs)을 쉽게 분해하는 미생물을 복합적으로 이용하여 탄소수가 적은 물질로 만든다면 PAHs 물질을 분해하는 효과가 더욱 클 것으로 사료된다. 4. 본 연구를 통해 토양 시료로부터 분리된 미생물이 탄소원으로 이용되는 물질 중, PAHs 물질을 이용하여 증식할 수 있다는 것을 알게 되었다. 이와 같이 유류계 유사물질을 탄소원으로 이용하는 Acinetobacter sp.와 유사한 미생물을 자연계로부터 탐색하여 응용함으로써 오염된 환경의 정화 및 환경오염 방제를 유도할 수 있을 것으로 사료되며, 환경학적으로 안정된 생물학적 처리 기술 개발을 위한 기초연구로서 활용 가치가 높다. This study was carried out to isolate soil microorganisms, which can emulsify crude oil by using liquid culture including crude oil (2% v/v). The strain 35 of them was screened to have the most significant biodegradable activity and oil conversion rate. Strain 35 was identified morphological, biological and physiological characteristics with 35, as Acinetobacter lwoffii. The strain 35 showed the crude oil emulsified activity, crude oil degradable activity, respectively. As a result of cell growth and emulsifying activity as a function of time were also determined. Crude oil degradation and the reduction of product peaks were identified by the analysis of resident oil by gas chromatograph and mass spectrometry. Thus it is belived that Acinetobacter lwoffii 35 use a variety of hydrogen carbonate as carbon resource.