원발성 간암환자에서 관찰된 99mTc - DISIDA의 비장 섭취

윤성철(Sung Chul Yun),이예봉(Ye Bong Lee),김성진(Sung Jin Kim),박언휘(Eun Hwi Park),방인숙(In Sook Bang),김홍주(Heung Joo Kim),이원재(Won Jae Lee) 대한핵의학회 1993 핵의학 분자영상 Vol.27 No.1

We report a case of hepatorna which had a suggested splenic metastasis in a 64 year-old male patient. Hepatoma proved by abdominal CT (Cornputed Tomography) and considerable accumulation of 99mTc-DISIDA was noted in a enlarged sp]een. We suggests the splenic uptake of the radionuclide corresponded to the diffuse metasfasis of hepatorna to spleen.

가토 압력과부하 심근비후에서의 Nuclear DNA 의 변화

김도헌(Do Heon Kim),이예봉(Ye Bong Lee),한동선(Dong Sun Han),이학중(Hak Choong Lee),박이태(Yee Tae Park),김성숙(Sung Sook Kim) 대한내과학회 1994 대한내과학회지 Vol.47 No.3

Background: During the development of cardiac hypertrophy, cytoplasmic contents of cardiac myocytes usually increase and nuclei of myocytes change as well. We used flow cytometry to study the changes of nuclear DNA in cardiac hypertrophy which was experimentally produced by aortic banding in New Zealand White Rabbits. This study was aimed to see how early these nuclear changes can occur and whether these changes correlate to the degree of cardiac hypertrophy. Methods: Each two rabbits were sacrificed at 0-,4-,8-,12- hours, and 1-,2-,4-,5-, and 9- days after experimental aortic constriction and their hearts were studied with flow cytometric analysis. Nuclear DNA was analyzed with FACScan (Becton-Dickinson Co.) after tissue preparatian using modified Hedley and Vindelov method, and staining with Krishan staining buffer. Results: The heart weight to body weight ratio (g/kg) increased progressively as a results of time lapsing after aortic banding. In control hearts (Group I ), flow cytometric analysie showed normal Diploidy pattern in all tissues. In hypertrophied hearts, Proliferative Indicies (S+GM Phase) were increased progressively in Group II (4, 8, 12 hours after aortic banding) and Group III (24 hours to 9 days after aortic banding). An Aneuploidy pattern was noticed in a severely hypertrophied heart produced 9 days aortic banding. Conclusions: Changes of nuclear DNA developed even in the early times in the experimental model of pressureoverload cardiac hypertrophy of rabbit after aortic banding and it was roughly correlated with the degree of heart hypertrophy. Abnormal DNA ploidy pattern can be observed in the severely hypertrophied heart.

과인슐린혈증을 보인 Nesidioblastosis 1 예

구정완(Jung Wan Ku),정민영(Min Young Jeong),김종목(Jong Mog Kim),김탁관(Tak Gwan Kim),윤형진(Hyung Jin Yun),박성진(Seong Jin Park),이예봉(Ye Bong Lee),이정호(Jeong Ho Lee),서정욱(Joung Wook Suh),김성숙(Sung Sook Kim),김정란(Jung Ran 대한내과학회 1994 대한내과학회지 Vol.47 No.5

Nesidioblastosis, a condition characterized by diffuse islet cell hyperplasia arising from the ductal epithelium, is often associated with hyperinsulinemic hypoglycemia. This is the most common cause of persistent neonatal hypoglycemia and is extremely rare in adults. A 38-year-old man had suffered from intermittent hypolycemic symptoms for 5 years, and had been relieved by carbohydrate ingestion. Plasma immunoreactive insulin levels and I/G ratio were inappropriately high during prolonged fast. Abdominal CT scan, angiography, and frozen section provided no evidence of a tumor. Pathologic examination of the resected pancreas revealed irregularly sized islets and scattering of small endocrine cell clusters throughout the acinar tissue and ductuloinsular complex and distinct islet cell hypertrophy are present.

비후된 심근에서의 유세포 분석을 통한 Nuclear DNA 의 관찰

김성진(Sung Jin Kim),방인숙(In Sook Bang),박언휘(Eun Hwi Park),김홍주(Heung Joo Kim),김도헌(De Heon Kim),이예봉(Ye Bong Lee),윤성철(Sung Chul Yun),한동선(Dong Sun Han),김성숙(Sung Sook Kim) 대한내과학회 1993 대한내과학회지 Vol.45 No.2

Background: During the development of the cardiac hypertrophy, cytoplasmic contents of cardiac myocyte usually increase, but the changes in the nucleus of myocytes are not well understood. Therefore, we used flow cytometry to study the changes of nuclear DNA in cardiac hypertrophy from human autopsy heart tissue. Besides, we also tried to see the DNA ploidy of developing heart of fetus. Method: Nine hypertrophied human hearts ranging from 350-620 g (Group II: 350-500 g, Group III: > 500 g) and 9 control hearts (Group I: 250-350 g) which we could obtain after the legal autopsy were studied with flow cytometric analysis. And 3 fetal hearts of 20-24 gestational weeks (Group IV) were also studied. Nuclear DNA was analyzed with FACScan (Becton-Dickinson Co.) after tissue preparation using Modified Hedley and Vindelov Method and staining with Krishan staining buffer. Results: In control hearts (Group I), Flow cytometric analysis showed normal Diploidy pattern in all tissue. However, in cardiac hypertrophy, Non-Diploidy pattern was predominant (3 out of 5 specimen in Group II, and all 4 specimen in Group III) and Tetraploidy was usually observed in this abnormal ploidy pattern, but Aneuploidy was also seen in 2 cases of severe hypertrophy of Group III. Proliferative indexes increased progressively in Group I, II, and III (17.8±5.55, 24.8±7.4%, and 36.4±5.2%, respectively) (p<0.05 between Group I and II, and p<0.01 between Group I k III). Normal growing heart tissue of fetus showed normal Diploidy pattern. Conclusions: In cardiac hypertrophy, abnormal nuclear DNA ploidy pattern can be obseved. This finding might say that, during the genesis of cardiac hyper- trophy, not only cytoplasmic change but also DNA synthesis in the nucleus occurs, but G2 phase arrest happens without further mitosis.

혈액투석요법 시행 중인 만성 신부전환자에서 혈청 갑상선 홀몬농도에 관한 연구

이정호,이예봉,윤성철 동국대학교 경주대학 1993 東國論集 Vol.12 No.-

To investigate the thyroid hormonal changes and their mechanisms in patients with chronic renal failure, we measured serum levels of the thyroid hormones using rediommunoassay and calculated each ratio of serum thyroid hormones in 20 patients with chronic renal failure who were on hemodialysis therapy. The following results were obtained. 1) The serum T_3 concentrations was significantly lower in patients with chronic renal failure(0.75±0.23㎍/l) than in normal subjects(1.18±0.37㎍/l). 2) The serum T_4 concentrations was significantly lower in patients with CRF(4.96±1.20㎍/l) than in normal subjects(8.45±1.55㎍/dl). 3) The serum FT_4 concentrations was significantly lower in patients with CRF(0.69±0.22ηg/ml) than in normal subjects(1.18±0.15ηg/ml). 4) The serum TSH concentrations in patients with CRF (2.08±2.38μμ/ml) tends to be greater than normal subjects(1.65±0.79μu/ml). But it was not significant statistically. 5) The serum rT_3 concentrations in patients with CRF (21.95±14.79ηg/ml) tends to be greater than normal subjects(18.5±2.80ηg/ml). But it was not significant statistically. 6) The calculated serum concentration ratios of T_3/T_4 and rT_3/T_3 in patients with CRF(0.17±0.09 and 38.30±43.31 : presented as Mean±SEM) dis not differ significantly that of normal subjects(0.14±0.04 and 17.04±5.71). But the serum concentration ratio of rT_3, T_3 in patients with CRF(4.53±2.73) was significantly greater than in normal subjects(2.25±0.50) 7) There was no significant difference in thyroid functions between male & female patients with CRF. 8) The serum T_3, T_4, TSH and rT_3 concentrations not related to the duration of hemodialysis, but serum FT_4 concentrations was lower in patients treated with hemodialysis for more than 36monts(0.49±0.10ηg/ml) than in patients treated with hemodialysis for less than 36months(0.74±0.22ηg/ml). It was concluded that the serum T_3, T_4 & FT_4 concentrations were significantly decreased, while calculated serum concentration ratios of rT_3/T_4 was significantly increased in patients with CRF. Thus we estimates that this decreased thyroid function in patients with CRF may reflect the alteration of serum rT_3 metabolism or increased peripheral conversion of T_4 to rT_3. It is also estimated that chronic hemodialysis leads to a propotional decline of thyroid hormone.

굴곡성 기관지내시경검사에 의한 폐질환의 진단

이영현,이예봉,김종목 東國大學校醫學硏究所 1993 東國醫學 Vol.1 No.-

저자들은 1989년 12월부터 1991년 6월까지 1년 7개월 동안 동국대학교 포항병원 내과와, 1991년 11월부터 1992년 12월까지 1년 1개월간에 동국대학교 의과대학 경주병원 내과에 내원한 폐질환 환자 247명을 대상으로 굴곡성기관지내시경을 사용하여 검사한 바 다음과 같은 성적을 얻었다. 1) 기관지내시경술을 받은 247명의 환자중 폐암 59례, 폐결핵 및 기관지결핵 58례, 폐염 35례, 기관지염 33례, 우측중엽증후군 11례, 농흉 및 늑막염이 9례, 미만성간질성질환 6례, 기관지 확장 증 5례, 폐농양 4례 순이었다. 2) 총 59예의 폐암 환자중 기관지내시경상에서 이상소견을 보였던 50례중 조직학적으로 확진된 경우는 38례(64.4%)였다. 폐암의 조직학적 분류를 보면 편평상피암이 36례(61%)로 가장 많았고, 남녀비는 5 : 1이었으며 호발연령은 50∼60대가 가장 많았고 호발부위는 양쪽 상엽기관지였다. 3) 폐암을 제외한 다른 염증성 폐질환에서는 기관지염과 폐염에서의 기관지 내벽은 부종과 충혈을 내공은 정상소견을 보이고, 폐결핵과 기관지확장증에서는 벽은 부종, 충혈, 삼출액 및 출혈이 있었고 내공은 협착과 변형을 보였다. 이중 결핵성 병변을 보면 폐결핵이 40례, 기관지결핵이 18례였는데 객담의 항산균도말은 39.5% 양성이었고 항산균 배양은 50% 양성이었으며 조직생검으로는 31,3%가 진단되었다. It is well known that flexible fiberoptic bronchoscopic examination is one of the best available method for the diagnosis of various pulmonary diseases. For the assessment of diagnostic yield, we analyzed 247 patients of various pulmonary diseases who had taken flexible bronchofiberoscopy in the Department of Internal Medicine, DongGuk University Medical School for the 36 months from December, 1989 to December, 1992. The results are summarized as follows: 1. Final diagnosis of the 247 cases was classified as follows: lung cancer 59 cases, tuberculosis 58 cases, pneumonia 35 cases, bronchitis 33 cases, RML syndrome 11 cases, empyema and pleurisy 9 cases, diffuse interstitial lung disease 6 cases, bronchiectasis 5 cases and lung abscess 4 cases. 2. The classification of primary lung cancer confirmed histologically was squamous cell carcinoma 36 cases(61%), adenocarcinoma 5 cases(8.5%), small cell carcinoma 12 cases (20.3%), undifferentiated carcinoma 4 cases(6.8%) and unditermined carcinoma 2 cases(3.4%). 3. The diagnostic yield of fiberoptic bronchoscopy for lung cancer was 86.7% and the histological confirm rate was 64.4%. 4. In lung cancer cases, the most prevalent ages was 5th∼6th decades and male to female ratio 5 : 1, the localization of chest film, right(39 cases, 66.1%) was more than left(18 cases, 30.5%) and the most frequent site was right upper lung field(16 cases, 27.1%). 5. We could not differentiated the various inflammatory lung diseases by bronchofiberscopy only. But in the bronchofiberscopic examination of various inflammatory lung diseases, we observed the nature of the wall and lumen of bronchus resulting as follows : in bronchitis and pneumonia, the bronchial wall disclosed edema and hyperemia, and the bronchial lumen showed normal finding; in pulmonary tuberculosis and bronchiectasis, the bronchial wall showed edema, hyperemia, exudate, and the bronchial lumen revealed stenosis and deformity, especially luminal widening was a characteristic finding in bronchiectasis; Lung abscess had purulent discharge on the bronchial wall and showed normal lumen of the bronchus. 6. Of the total tuberculosis 58 cases, 40 cases were pulmonary tuberculosis and 18 cases were endobronchial tuberculosis. The tuberculosis was confirmed by sputum AFB smear in 39.5%, AFB culture in 50%, biopsy in 31.3%. In our country with higher prevalence rate of tuberculosis it is important to find out the cases with endobronchial tuberculosis early for prevention of permanent bronchial stenosis.

유체의 耐寒性 및 低 Glucosinolate 形質 改良

강광희,김재환,이예봉 영남대학교 자원문제연구소 1993 資源問題硏究 Vol.12 No.-

The purpose of this paper is to improve new lines of cold damage tolerance and low glucosinolate(GSL) content from four combination AX B, AX C, AX D and AXE. Domestic line A was tolerant to cold damage and high GSL(78.3 umol/g dry meal) , while introduced lines B, C, D and I were susceptible to winter cold and low GSL(13.6-31.3 umol/g dry meal)content. In F2 generation, the variance of cold damage tolerance and GSL content were not stable. Thus, it was possible to select some lines which had cold damage tolerance and low GSL content. From the field experiment, selected lines turned out to have tolerant winter hardiness and low GSL content less than 30 fmol/g dry meal, and other characteristics for yield were desirable. The new rapeseed varities which were selected by this procedure could be expanded to the higher latitude area.

경북지역 알레르기 환자에서 피부단자검사,혈청 총 면역 글로부린 E치 및 말초혈액 호산구수의 측정

김규회,박기흠,이예봉,양창헌,이창우,이영현 대한가정의학회 1993 Korean Journal of Family Medicine Vol.14 No.3