유물의 보존환경에 대한 생물학적 조사 연구

이명혜,이규식,한성희,안희균,Lee, Myung-Hye,Lee, Kyu-Shik,Han, Sung-Hee,Ahn, Hee-Kyun 국립문화재연구소 1992 保存科學硏究 Vol.13 No.-

We made biological investigation on the conservational environment of collections in the Ho Am museum. Annual average temperature and relative humidity outside the museum were $11. 0∼11.7^{\circ}C$ and 64.8∼74.4% respectivey. On the other hand, average annual temperature and relative humidity inside the main storage were $19.1∼20.1^{\circ}C$ and 53.0∼63.4%. We isolated fungi and classified into 8 genus 13species fungi and selected four fungi having high cellulotic activity such as Alternaria brassicae KCPRI 9202, Aspergillus niger KCPRI 9205, Aspergillusversicolor KCPRI 9206, Penicillium adametzi KCPRI 9208. These fungi were examined on the posibility of collections being damaged under current conservation al environment in the museum. KCPRI 9208 was non-tonophilic fungus and other were facutative tonophilic fungi. These showed maximal cellulotic activity of enzymeshaking culture at pH 5.0∼5.5 for 4 and 5 days. In proprtion to the period damaged, cellulase activity for paper damaged artifically with growing worse of material. As are sult cellulotic activity by fungi increased.

볶음 공정이 결명자의 항산화 활성에 미치는 영향

이명혜(Myung-Hye Lee),조진호(Jin-Ho Cho),김범근(Bum-Keun Kim) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.5

본 연구에서는 결명자의 볶음 온도 및 볶음 시간에 따른 색도변화를 살펴보고, 이들의 물 추출물에 대한 총 폴리페놀 함량, 전자공여능을 측정하였다. 결명자의 볶음 온도는 175, 200, 225 및 250℃로 하였으며 볶음시간은 각각의 온도에서 5분 및 10분 동안 수행하였다. 총 폴리페놀 함량의 경우 250℃를 제외하고는 모든 볶음 온도에서 시간에 따라 증가하는 경향을 나타내었으나, 250℃의 경우 10분 동안 볶음처리를 할 경우 그 함량이 크게 감소하였다. 항산화능 측정 결과 200℃ 이하의 온도에서 볶음처리를 할 경우 볶음시간에 따라 그 활성이 증가되거나 유지되는 것을 볼 수 있었으나, 225℃ 및 250℃의 경우 5분간 볶음처리하였을 때 그 값이 오히려 200℃의 것보다 낮게 나타났으며, 시간에 따라 증가폭이 적거나 오히려 감소하는 경향을 나타내었다. 색도측정 결과 175℃ 및 200℃에서는 볶음시간에 관계없이 높은 값을 나타내었으나, 225℃ 및 250℃에서는 10분간 볶음처리할 경우 탄화되어버리는 것을 확인할 수 있었다. 이와 같은 결과를 통해 볶음 온도와 시간이 결명자차 혹은 결명자 가공제품의 제조에 있어서 그 품질을 결정하는 중요한 요인인 것을 확인하였으며, 이들이 제품에 미치는 영향을 규명하는 것이 새로운 소재에 대한 적용뿐만 아니라 품질의 고급화를 위하여 반드시 필요한 연구라고 판단된다. The effects of roasting temperature and time on the antioxidant activities of Cassia tora L. were investigated. In comparison with raw seeds (7.15 ㎎ TAE/g), roasted seeds contained a significantly higher total polyphenol content (p<0.05). However, seeds roasted at a higher temperature (250℃) for 10 min showed a significantly lower total polyphenol content (2.30 mg TAE/g; p<0.05). The electron donating abilities of Cassia tora L. seeds increased with an increase in roasting time; further, seeds roasted for 5 min at lower temperatures showed higher electron donating abilities (80.61% at 175℃; 80.75% at 200℃) than did seeds roasted for 5 min at higher temperatures (76.26% at 225℃; 77.35% at 250℃). Seeds roasted at lower temperatures showed adequate L values, regardless of roasting time; by contrast, seeds roasted for 10 min at higher temperatures, showed markedly lower L values. Our results indicate that roasting temperature and time must be controlled to produce high-quality Cassia tora L. products.

볶음 공정에 따른 타타리 메밀의 항산화 활성 측정

이명혜(Myung-Hye Lee),조진호(Jin-Ho Cho),김종찬(Jong-Chan Kim),김범근(Bum-Keun Kim) 한국식품과학회 2014 한국식품과학회지 Vol.46 No.3

본 연구에서는 타타리 메밀의 볶음 온도 및 볶음 시간에 따른 색도변화를 살펴보고, 이들의 물 추출물에 대한 총 페놀성 화합물 함량, 전자공여능을 측정하였다. 총 페놀성 화합물 함량 측정결과, 볶음 처리를 하지 않은 타타리 메밀에 비하여 10분 처리구의 경우 225℃와 250℃에서는 총 페놀성 화합물 함량이 유의적으로 크게 감소하였다. 전자공여능 측정 결과 175℃와 200℃의 경우 볶음시간에 따라 증가하는 경향을 나타낸 반면, 225℃와 250℃의 경우 볶음시간이 길어질수록 전자공여능 값이 감소하는 경향을 나타내었다. 색도 측정 결과, 볶음 처리를 하지 않은 타타리 메밀의 L값과 비교하여 200℃와 250℃에서 10분동안 볶음 처리를 한 경우 탄화되어 크게 감소하는 것을 확인할 수 있었다. 이와 같은 결과를 통해 타타리 메밀을 이용한 가공제품의 품질 고급화를 위하여 최적의 볶음 조건 설정은 매우 중요한 연구라고 판단된다. The effects of roasting temperature and time on the antioxidant activities of tartary buckwheat were investigated. Compared to raw seeds (2.05 mg TAE/g), seeds roasted at 175℃ for 5 min showed significantly higher total polyphenol content (p<0.05), while those roasted at 250℃ for 10 min showed significantly lower total polyphenol content (2.77 and 2.56 mg TAE/g, respectively). The electron-donating abilities of tartary buckwheat seeds increased with an increase in the roasting time at lower temperatures (p<0.05). However, seeds roasted at a higher temperatures (250℃) for 10 min showed significantly lower electron-donating abilities (p<0.05). Seeds roasted at 175℃ showed adequate L values, regardless of the roasting time. In contrast, seeds roasted for 10 min at 250℃, showed markedly lower L values. Our results suggest that the roasting temperature and time must be controlled to produce high-quality tartary buckwheat products.

Xanthomonas campestris M12에 의한 포화 탄화수소의 생분해

최순영,이명혜,황문옥,민경희,Choi, Soon-Young,Lee, Myung-Hye,Hwang, Moon-Ok,Min, Kyung-Hee The Microbiological Society of Korea 1994 미생물학회지 Vol.32 No.2

Xanthomonas campestris M12는 octane을 분해할 수 있는 OCT 플라스미드를 갖고 있으며, 이 플라스미드의 분해 능력으로 $C_8$에서 $C_{16}$까지의 n-alkane을 이용할 수 있었다. X. campestris M12 균주는 n-alkane을 이용하여 alcohol, aldehyde, fatty acid를 거쳐서 분해할 수 있으나 heanoic acid, adipic acid, pimelic acid 그리고 heptanal은 이용할 수 없었다. 또한 이 균주는 straight fatty acids의 monoterminal oxidation과 diterminal oxidation에 의하여 n-alkane을 분해할 수 있으며, branched-chain alkane도 이용할 수 있었다. Xanthomonas campestris M12 carrying OCT plasmid which could dissimilate octane was able to utilize n-alkanes of eight to sixteen carbon atoms via the capacity of this plasmid. M12 strain could utilize terminal oxidation products of these primary, alkanes, alcohols, aldehydes and fatty acids but not hexanoic acid, adipic acid, pimelic acid and heptanal. This strain also biodegraded n-alkanes by monoterminal or diterminal oxdation of straight-chain fatty acids, and branched-chain alkane.

흡연이 폐환기능에 미치는 급성효과에 관한 연구

이영현 ( Young Hyun Lee ),이명혜 ( Myung Hye Lee ),최병휘 ( Byoung Whyui Choi ),박춘식 ( Chun Sik Park ),심영수 ( Young Soo Shim ),김건열 ( Keun Youl Kim ),한용철 ( Young Chul Han ) 대한결핵 및 호흡기학회 1982 Tuberculosis and Respiratory Diseases Vol.29 No.4

이종이식된 인체대장암에서 항 태아성암항원 단세포군항체 IgG 및 F ( ab` ) 2 분절을 이용한 방사면역 검출법

고창순(Chang Soon Koh),신성해(Sung Hae Shin),문대혁(Dae Hyuk Moon),이명혜(Myung Hye Lee),이동수(Dong Soo Lee),정준기(June Key Chung),이명철(Myung Chul Lee) 대한내과학회 1991 대한내과학회지 Vol.40 No.2

N/A This study was designed to investigate whether specific monoclonal antibody against carcinoembryonic antigen (CEA-92) and F (ab`)2 fragments of CEA-92 can be used for the scintigraphic localization of human colon cancer xenograft on nude mice models. Intact CEA-92 IgG and F (ab`)2 fragments of CEA-92 were radiolabeled with Iodine-131, then injected intraperitoneally into nude mice bearing human colon cancer (SNU-C4). the scintigrams were obtained at intervals of one day. The digital computer images were also obtained to analyze quantitatively the tumor contrast and biological half life of radiolabeled antibody in tumor tissue. The paired labeling experiments, in which I-131-labeled anti-CEA antibody or it's fragments and I-125-labeled control IgG or it's fragments were injected simultaneously, were undertaken to determine the specific localization of CEA-92 IgG and F (ab`)2 fragments of CEA-92. Tumor uptake of intact CEA-92 expressed in % injected dose per gram tissue (% ID/g tissue) was 2.95 at day 2 and 1.57 at day 5 which were significantly higher than those in other tissues (p<0.05). However % injected dose in blood also showed high uptake (3.08 at day 2, 1.29 at day 5) and there was no significant difference between blood and tumor tissue. In case of F (ab`) 2 fragments, tumor uptake was 2.52 and 0.70 at day 2 and 5, respectively. In contrast to intact CEA-92, all other tissues including blood had significantly lower antibody uptakes than tumor. The antibody specific localization ratios of F (ab`) 2 fragments of CEA-92 at day 2 were ranged from 4.77 to 8.40 which were significantly higher than those at day 5 (1.89-5.15, p<0.05) and localization ratios of intact CEA-92 IgG at day 2 (1.37-2.98, p<0.01). The scintigrams clearly demonstrated selective localization of radioactivity over transplanted tumor with-out and additional image processings. With F(ab`)2 fragments, more rapid and clear tumor images were obtained. Best tumor contrast as quantitated by analyzing digital computer scans was obtained between day 4 and 5 after the antibody injection. Biologic half life of the intact CEA-92 in tumor tissue was significantly longer than that of F (ab`)2 fragments (3.89 day vs 2.19 day, p<0.005). We concluded that the monoclonal antibody CEA-92 was localized selectively and specifically in human colon carcinoma tissue and could be used in radiommunodetection in xenograft model. The F(ab`)2 fragments were found to give better and more rapid specific tumor localization than intact CEA-92.

OCT 플라스미드를 갖는 원유 분해세균에 의한 Octane 분해능

최순영,김창숙,이명혜,황문옥,민경희 숙명여자대학교 자연과학연구소 1991 자연과학논문집 Vol.- No.2

원유 분해세균에 의한 원유 분해능을 조사한 결과, Xanthomonas campestris M12. Xanthomonas sp. M28, Acinetobacter lwoffi G1, and Klebsiella pneumoniae L25, 그리고 Pseudomonas maltophilia N246 등의 순서로 나타났다. Xanthomonas campestris M12, Xanthomonas sp. M28, 그리고 Pseudomonas maltophilia N246 균주 모두 octane 분해시의 온도는 30℃에서 최적이었으며, 최적 pH는 X. campestris M12와 Xanthomonas sp. M28이 7.0∼7.5이었고, P. maltophilia N246이 7.5∼9.0이었다. N246 균주의 최적 NaCl 농도는 3.0∼3.5%이었다. P. maltophilia N246와 X. campestris M12는 모두 플라스미드를 갖고 있음을 확인하였고, N246 균주로부터 플라스미드를 제거하였을 경우 octane 분해능이 소실되었으므로 이 플라스미드 위에 octane 분해 유전자가 있음이 확인되었다. 이 균주의 OCT 플라스미드의 크기는 118kb이었다. 또한, N246 균주는 ampicillin 항생제에 내성을 나타내었다. Xanthomonas campestris M12. Xanthomonas sp. M28. Acinetobacter lwoffi G1, and Klebsiella pneumoniae L25, Pseudomonas maltophilia N246 were screened to increase the ability for crude oil utilization. All of these could utilize hexadecane and octane with the exception of N 246 strain for only octane biodegradation. Thus N246, M12, and M28, strains were specially examined for octane oxidation. Octane biodegradation by three strains showed the optimal conditions at 30C, pH7.0∼9.0 and 0.2∼0.3% octane concentration as a substrate. It was found that P. maltophila N246 and X. campestris M12 had plasmid and the cured plasmid from N246 strain lost octane utilization. Therefore, it was confirmed that certain genes for octane utilization were located on OCT plasmid in N246 strain. The size of OCT plasmid in N246 strain was 118 kb. The N246 strain was resistant to ampicillin.

Xanthomonas campestris 에 의한 원유분해에 미치는 원유성분과 온도의 영향

최순영,이명혜,황문옥,이창섭,민경희 한국물환경학회 1991 한국물환경학회지 Vol.7 No.1

Four crude oils (Arabian light, Iran light, Oman, Libya) of different chemical composition were tested as to their biodegradability under mesophilic condition. Changes of the chemical composition of the crude oils by Xanthomonas campestris M12 were monitored using the gas-liquid chromatography. The ability of X. campestris M12 to use crude oil as a sole carbon source was not dependent on the composition of the n-saturate or aromatic fraction, but on that of the asphaltic fraction. The biodegradability of the Arabian light crude oil was found to be highly dependent on an incubation temperature. It was shown that addition of 0.1 % tween 80 to the incubation medium increased bacterial growth and biodegradability at 30℃ rather than that at 37℃.

OCT 플라스미드를 갖는 원유 분해세균에 의한 Octane 분해능

최순영,김창숙,이명혜,황문옥,민경희 한국산업미생물학회 1991 한국미생물·생명공학회지 Vol.19 No.1

원유 분해세균에 의한 원유 분해능을 조사한 결과, Xanthomonas campestris M12, Xanthomonas sp. M28, Acintobacter lwoffi G1, Klebsiella pneumoniae L25, 그리고 Pseudomonas maltophilia N246 등의 순서로 나타났다. Xanthomonas campestris M12, Xanthomonas sp. M28, 그리고 Pseudomonas maltophilia N246 균주 모두 octane 분해시의 온도는 30℃에서 최적이었으며, 최적 pH는 X. campestris M12와 Xanthomonas sp. M28이 7.0∼7.5이었고, P. maltophilia N246이 7.5∼9.0이었다. N246 균주의 최적 NaCl 농도는 3.0∼3.5%이었다. P. maltophilia N246와 X. campestris M12는 모두 플라스미드를 갖고 있음을 확인하였고, N246 균주로부터 플라스미드를 제거하였을 경우 octane 분해능이 소실되었으므로 이 플라스미드 위에 octane 분해 유전자가 있음이 확인되었다. 이 균주의 OCT 플라스미드의 크기는 118kb이었다. 또한, N246 균주는 ampicillin 항생제에 내성을 나타내었다. Xanthomonas campestris M12, Xanthomonas sp. M28, Acinetobacter lwoffi G1, and Klebsiella pneumoniae L25, Pseudomonas maltophilia N246 were screened to increase the ability for crude oil utilization. All of these could utilize hexadecane and octane with the exception of N246 strain for only octane biodegradation. Thus N246, M12, and M28, strains were specially examined for octane oxidation. Octane biodegradation by three strains showed the optimal conditions at 30℃, pH 7.0∼9.0, and 0.2∼0.3% octane concentration as a substrate. It was found that P. maltophila N246 and X. campestris M12 had plasmid and the cured plasmid from N246 strain lost octane uitilization. Therefore, it was confirmed that certain genes for octane utilization were located on OCT plasmid in N246 strain. The size of OCT plasmid in N246 strain was 118 kb. The N246 strain was resistant to ampicillin.