Purification and Characterization of Lacticin NK34 Produced by Lactococcus lactis NK34 against Bovine Mastitis

이나경,박여랑,김현욱,박용호,임성렬,김종만,김재명,남향미,정석찬,백현동,Lee, Na-Kyoung,Park, Yeo-Lang,Kim, Hyoun-Wook,Park, Yong-Ho,Rhim, Seong-Lyul,Kim, Jong-Man,Kim, Jae-Myung,Nam, Hyang-Mi,Jung, Suk-Chan,Paik, Hyun-Dong Korean Society for Food Science of Animal Resource 2008 한국축산식품학회지 Vol.28 No.4

Lactococcus lactis NK34, isolated from jeotgal (Korean traditional fermented fish), produces bacteriocin against bovine mastitis pathogens such as Staphylococcus aureus 7, S. aureus 8, Staphylococcus chromogenes 10, S. chromogenes 19, Staphylococcus hominis 9, Streptococcus uberis E290, Enterococcus faecium E372, Streptococcus agalactiae ATCC 13813, Pseudonocardia autotrophia KCTC 9455, and Staphylococcus simulans 78. Lacticin NK34 was inactivated by protease XIV but not by protease IX, protease XIII, proteinase K, $\acute{a}$-chymotrypsin, trypsin, and pepsin. Also, lacticin NK34 was stable over a pH range of 2 to 9 for 4 hr and withstood exposure to temperatures of 30-$100^{\circ}C$ for 30 min. Lacticin NK34 showed bactericidal effects against S. simulans 78. This bacteriocin was purified using ammonium sulfate precipitation, ion exchange chromatography, ultrafiltration, and hydrophobic chromatography. Tricin-SDS-PAGE of purified bacteriocin gave the same molecular weight (3.5 kDa) as nisin. The gene encoding this bacteriocin was amplified by PCR using nisin gene-specific primers. It showed similar sequences to this nisin Z gene. These results indicate that lacticin NK34 is a nisin-like bacteriocin, and could be used as an antimicrobial alternative for livestock.

축산업 분야에서의 박테리오신의 산업적 이용 및 향후 전망

이나경,이주연,곽형근,백현동,Lee, Na-Kyoung,Lee, Joo-Yeon,Kwak, Hyung-Geun,Paik, Hyun-Dong 한국축산식품학회 2008 한국축산식품학회지 Vol.28 No.1

More safe and natural food was recently needed by consumers. Antimicrobials including sodium azide, penicillin, and vancomycin were used for therapeutic agents against pathogens such as Listeria monocytogenes, Staphylococcus aureus, Escherichia coli O157:H7 in dairy and meat industry. These antimicrobials and preservatives were prohibited in stock farm and food because they were caused resistant strain and side effects. Bacteriocins are proteinaceous compounds that may present antimicrobial activity towards important food-borne pathogens and spoilage-related microflora. Therefore, bacteriocins were reported as an alternative of antimicrobials. Due to these properties, bacteriocin-producing strains or purified bacteriocins have a great potential of use in biologically based food preservation systems. Despite the growing number of articles regarding on the isolation of bacteriocinogenic strains, genetic determinants for production, purification and biochemical characterization of these inhibitory substances, there are only limited reports of successful application of bacteriocins to dairy and meats. This review describes bacteriocins related to dairy and meat products for the further use.

여러 분리원으로부터 유방염 원인균에 대한 항균력을 가진 유산균의 분리

이나경,최인애,박용호,김종만,김재명,정석찬,백현동,Lee, Na-Kyoung,Choi, In-Ae,Park, Yong-Ho,Kim, Jong-Man,Kim, Jae-Myung,Jung, Suk-Chan,Paik, Hyun-Dong 한국축산식품학회 2007 한국축산식품학회지 Vol.27 No.4

본 연구에서는 원유와 사일리지, 전통발효식품 등으로부터 유방염 원인균에 대한 항균성을 가지는 유산균을 분리하였고, 이들 중 원유에서는 79, 91-3 균주와 전통발효식품에서는 SA131, NK24, NK34, 32, 44-1 균주, 사일리지에서는 253, 261, 262, 263, 265 균주, 닭분변에서 B32, C23 균주에서 유방염 원인균에 대한 비교적 높은 항균력을 확인할 수 있었다. 이를 통해 새로운 항생제 대체물질의 가능성을 확인할 수 있었다. Bovine mastitis is costly infectious disease of dairy cattle, being responsible for significant economic losses all over the world. Also, mastitis has troubled about resistance to antibiotics. The purpose of this study was to screen a novel antimicrobial strain from various sources (raw milk and feeds (from farm of Paju, Dangjin, and Hwasung), commercial milk, Korean traditional fermented foods, and chicken feces). The isolate was screened using triple agar layer method and deferred method was used for confirmation of antimicrobial effect. Seventy six of isolates were screened using triple agar layer method. In these strain, 42 isolates were shown a broad spectrum of autimicrobial activity against mastitis pathogens. Especially, fourteen isolates were shown over 20 mm inhibition zone against S. aureous ATCC 25923. These results suggest that these novel antimicrobial strains could be used for the alternative of antibiotics.

Ovotransferrin 가수분해물의 Angiotensin-converting Enzyme 활성억제 효과 및 생산 최적화

이나경,안동욱,박근규,백현동,Lee, Na-Kyoung,Ahn, Dong-Uk,Park, Keun-Kyu,Paik, Hyun-Dong 한국축산식품학회 2010 한국축산식품학회지 Vol.30 No.2

Angiotensin-converting enzyme (ACE) inhibitory activity and production optimization of ovotransferrin hydrolysates were studied. Ovotransferrin was hydrolyzed by several enzymes (protamex, alcalase, trypsin, pepsin, neutrase, and flavorzyme) and acid (0.03 N HCl). Ovotransferrin hydrolysate reduced ACE activity by 60.2%, 55.8%, and 42.6% when treated with trypsin, acid, and pepsin, respectively. Trypsin was selected for production of peptide having maximum AC inhibitory effect, which was greatest with 7 h hydrolysis. Central composite design determined that optimum composition of ACE inhibitory substances using substrate concentration of 20-35%, temperature of $35-55^{\circ}C$, and pH of 6.0-8.0. The optimum composition was 1% trypsin, substrate concentration of 26.32%, $51.29^{\circ}C$, and pH 6.32. Under this conditions, a maximum ACE inhibitory effect of 69.1% was evident, similar to the predicted value.

일본에서의 외국인노동자의 현황과 법 정책 – 외국인노동자에 대한 노동안전위생법을 중심으로 –

이나경(Lee, Na-kyoung) 한국비교노동법학회 2021 노동법논총 Vol.53 No.-

감정지능을 활용한 간호학에서의 내러티브 문학수업

이나경(Na Kyoung Lee) 한국질적탐구학회 2018 질적탐구 Vol.4 No.2

급변하는 4차 산업혁명의 도래와 더불어 산업체제 전반이 미래 위기 대응을 위한 전략을 모색하고 있다. 이러한 시대의 흐름과 발맞추어, 간호학계에서도 미래 불확실성에 대처하려는 노력을 하고 있다. 도제방식 중심의 과거의 커리큘럼을 탈피하여 보다 인간적 측면을 강조하는 학문적 개선을 새로이 시도하고 있다. 그러한 측면에서 감정지능은 인간 고유의 정서를 강조하 는 역할로 간호학이 추구하는 돌봄의 본질과 맞닿아 있다. 돌봄의 맥락에서 인간의 생에 대한 총체적이며 연속성 있는 관리와 개개인의 다양한 감정 경험을 학습체계로 구현할 수 있는 방법중에 하나가 바로 문학 수업이다. 내러티브를 통한 문학수업은 일상적인 인간의 삶부터 위기상황의 비 일상성 모두를 아우를 수 있는 간접경험의 산실이다. 이 연구에서는 감정지능을 활용하여 내러티브를 통해 문학수업을 설계하고, 간호학과 학생들 5명을 대상으로 3월에서 5월까지 파일럿 연구를 진행하고 그들의 수업 경험을 통하여 질적 인터뷰와 포커스 인터뷰를 통해 자료를 수집하였다. 이 연구결과 도출된 주제는 ‘간호에 대한 인간다움으로의 인식 변화’였으며 하위 범주로는 ‘간호 대상자에 대한 사람 중심의 인식 변화’와 ‘공감능력이 높아지는 것을 느 낌’, ‘자기 성찰을 통한 깊이 있는 자기이해’, ‘의사소통의 맥락을 깊이 있게 이해함’이었다. 이 연구의 결과를 바탕으로 감정의 다양한 활용을 통해 전인간호의 기틀을 마련하고자 한다. With the rapid changes in the advent of the fourth industrial revolution, the entire industrial system is seeking a strategy to cope with the future crisis. Keeping with these trends, the nursing academy is also making efforts to cope with uncertainty by drawing up various future blueprints to address the concerns over substitution of human labor with robot. As a countermeasure to such problem, attempt for new academic improvement is made to put more emphasis on aspects of humanity by breaking away from the past curriculum that is based on the technical tradition of apprenticeship. Emotional intelligence, which comes from the possibility of being human, also plays a role in emphasizing human emotions, and it is in touch with the nature of caring that nursing is genuinely pursuing. This is one of the ways that we can realize continuous management of human life as a whole and various emotional experiences of individuals in a learning system. Narrative-based literature classes are the result of indirect experiences that can cover both everyday human life and the crisis situations out of the routines. In this study, we designed the literature class, based on the narrative in story, using the emotional intelligence, and conducted the pilot study with 5 students who agreed to the experiment to see if they could use it in the actual nursing studies to check the possibility of literacy classes with their experiences in class.

간호대학생이 지각한 자아존중감과 간호사 이미지가 임상실습 만족도에 미치는 영향: COVID-19 시기에 임상실습교육 대상자를 중심으로

이나경(Na Kyoung, Lee),안나원(Nawon, An) 한국간호연구학회 2021 한국간호연구학회지 Vol.5 No.2

Purpose : This study was conducted to identify the influence of self-esteem and the image of a nurse and its impact on satisfaction with clinical practice among nursing students who experienced clinical practice during the COVID-19 outbreak. Methods : Between September 30 to October 15, 2020, 128 nursing students in their third and fourth grade of K and C colleges completed a self-report structured questionnaire. The data were analyzed using descriptive statistics, t-test, ANOVA, Pearson’s correlation coefficients, and hierarchical regression analysis using the SPSS/WIN 22.0 program. Results : The mean score on the total clinical practice satisfaction was 3.52±0.43. Clinical practice satisfaction was higher in the fourth grade students than in the third grade students(p=.014) and showed a statistically significant positive correlation with self-esteem and the image of a nurse(p<.001). The variables explained the satisfaction with clinical practice by 29.3%. Conclusion : In order to increase the satisfaction with clinical practice of nursing students during the COVID-19 outbreak, it is necessary to develop educational interventions that strengthen positive images of nurses and increase self-esteem.

시공간 중심 서비스디자인 평가 모델 연구

이나경(Na-Kyoung Lee),반영환(Young-Hwan Pan) 한국디자인학회 2013 디자인학연구 Vol.26 No.1

한국전통건축 ‘채나눔’의 현대건축 적용 특성 분석 - 전통건축 개념의 형태적 변형을 중심으로 -

이나경(Lee, Na-Kyoung),정석연(Jung, Suk-Yon) 한국실내디자인학회 2024 한국실내디자인학회 학술대회논문집 Vol.26 No.1

Korean modern architecture is following the Western architectural style due to rapid modernization due to the influence of modernism based on functionalism. Therefore, efforts are needed to secure Korean tradition, such as modern reinterpretation of traditional architecture, and we must move in the direction of modernizing traditional architecture. In this study, we attempted to find out the direction of modernization by applying elements of traditional Korean architecture to modern architecture. A case study of modern architecture was conducted through theoretical consideration through previous research and the characteristics of chae-dividing among the elements of traditional architecture. Through the characteristics of Chae-dividing, we were able to find expressions of various elements through applicability to modern architecture and the ambiguity unique to Korean traditional architecture. Future research will need to add not only analysis, but also more accurate analysis of interior space and measures that can suggest the direction of modernization.

개성화를 잉태하고 있는 모성 상징으로서의 산

이나경(Lee Na Kyoung) 한국임상모래놀이치료학회 2015 상징과 모래놀이치료 Vol.6 No.1

In this article, maternal symbolism of the mountain conceiving individuation was examined in the context of analytical psychology. Three significant symbolic meanings were identified: the mountain as maternity, the mountain as the one that brings about ego development, and which induces individuation. The lexical definition and general characteristics of the mountain, the significance of the mountain in various cultures and myths, including Korea, as well as the meanings that were drawn from client s personal experience were analyzed. As a result of analysis, it was concluded that the mountain was an important symbol that encouraged ego development and helped one discover the mother complexes that were hidden in the deep abyss within the unconscious, so that it could lead to individuation.