http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
옥수수 - 대두박 사료내 미생물 Phytase 의 첨가가 브로일러의 생산능력에 미치는 영향
손광수,권관,권찬호,장재익,주종철,유문일,최양웅 한국축산학회 1999 한국축산학회지 Vol.41 No.5
This experiment was conducted to evaluate the effects of a domestic phytase originated from bacterial source on performance of starter and Bower broiler chicks. It was basically designed to meet NRC requirement for the contents of calcium and phosphorus when the contents of Ca and P in the feeds were met to 100%(Control; C) and 80%(P0) of the NRC requirement. Three different bacterial phytase(0, 300, 500PU) were supplemented to basal diet and compared it's effect on growth performance, nutritional digestibility, fecal N and P excretion, tibial Ca and P content. A total 200 broiler chicks were assigned to 4 treatments with 5 replications per treatment, 10 chicks of each replication. The weight gain(1,581.6g) in control treatment(C) was slightly higher than that of P0(1,557.7g). Although phytase treatments(P300 and P500) tended to show higher weight gain as compared to treatments without phytase, there were no significant differences among the treatments. No differences were observed among dietary treatments for feed efficiency although broiler chicks fed control diet (1.94) tended to show slightly better feed efficiency than those fed P0 diet(1.99). The excretion of P per kg weight gain tended to be decreased by 17% when 300PU of bacterial phytase was fed to starter and by 10% when 500PU was fed to Bower. However, there were no statistical significances among the treatments. It was concluded that the addition of phytase had neither affects in improving of utilization efficiencies of dry matter, crude protein, and crude fat, nor efficiencies of Ca and P. The supplemental of phytase at the level of 500 ppm for both stages of broiler chick diets was assumed to appropriate.
허태영,Quan Truong Hoang,Thuy Giang Nguyen Cao,오승환,유문철,심민석,최수형 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.6
RNAi-based therapeutics utilizing small interfering RNAs (siRNAs) are of significance in the clinic as it serves great potentials for gene-based treatment of human diseases. Currently, siRNA-based RNAi efficiency has been limited by facile degradation, poor cell membrane penetration, and short half-life time of siRNA. In this study, block copolyelectrolytes containing a poly(ethylene oxide) (PEO) neutral block and a cationic block were synthesized by anionic polymerization and post-polymerization modification. In the cationic block, guanidinium and ammonium groups were randomly incorporated with various fractions to achieve micelleplexes for safe and efficient siRNA delivery. Compared to traditional polyethylenimine-based polyplexes, all micelleplexes exhibited enhanced cellular internalization and better gene silencing efficiency with higher stability. As the fraction of guanidinium groups increased, the uptake level and siRNA transfection were enhanced due to stronger binding of guanidinium groups with siRNA. However, the trade-off between cellular internalization and toxicity was inevitable with increasing guanidinium fraction. The fraction of guanidinium group in block copolyelectrolytes was optimized by the systemic evaluation of cytotoxicity and gene silencing efficiency of the micelleplexes.