Furosemide의 가토 신세뇨관 수송에 대한 Cephalexin의 영향

염윤희,용재익,조규철 한림대학교의료원 1981 人間科學 Vol.5 No.3

Acetazolamide가 Furosemide의 이뇨작용에 미치는 영향

염윤희,이종화,김인순,김옥녀,조규철 大韓藥理學會 1981 대한약리학잡지 Vol.17 No.2

Furosemide의 이뇨작용에 대한 acetazolamide의 영향을 알아보고자 토끼를 4군으로 나누어 saline(0.5ml/kg), acetazolamide (10mg/kg), furosemide (0.5mg/kg), acetazolamide (10mg/kg)+furosemide(0.5mg/kg)를 각각 정맥주사하였다. 1) Acetazolamide와 furosemide 병합투여시 furosemide를 단독투여했을 때의 뇨량, 뇨중 전해질 배설량에 비하여 유의한 감소를 보였다. 2) Acetazolamide와 furosemide 병합투여시와 furosemide를 단독투여시의 뇨중 전해질과 뇨량의 fraxtional excretion rate를 비교하면, 병합투여시에 감소되어 나타났으며 뇨중전해질보다 뇨량에서 그 정도가 심하였다. 3) Acetazolamide와 furosemide 병합투여시 뇨 pH가 furosemide 단독투여시보다 높게 나타났다. 4) 뇨중 <TEX>$Cl^-$</TEX>배설량에 대한 <TEX>$Na^+\;+K^+$</TEX>배설량의 비는 acetazolamide + furosemide 병합투여군과 furosemide 단독투여군에서 유의한 차이를 보이지 않았다. Acetazolamide와의 병합투여로 나타나는 furosemide 이뇨작용의 감소는 뇨 pH의 증가 또는 ascending Henle‘s limb에서의 <TEX>$Cl^-$</TEX>재흡수 억제의 감소에 기인하는 것으로 사료된다. Effect of acetazolamide on the diuretic action of furosemide was investigated in rabbits. The rates of urine flow and excretion of salts were significantly reduced when furosemide (0.5 mg/kg) was administered with acetazolamide (10 mg/kg) compared to the diuretic response of the single furosemide (0. 5 mg/kg) administration. Reduction in the fractional excretion rate of urine volume was more pronounced than the fractional excretion rate of salts. The results suggest that reduction of diuretic action on furosemide by combined administration of acetazolamide is probably due elevated urinary pH and interference in the mechanism of inhibition of chloride transport in the ascending Henle's limb.

유기산이 Furosemide 의 흡수 및 신세뇨관 수송에 미치는 영향(Ⅲ) : PAS , PAH 의 영향

염윤희,용재익 한국약제학회 1983 Journal of Pharmaceutical Investigation Vol.13 No.1

유기산 약물중 P-aminosalicylic acid와 P-aminohippuric acid가 furosemide의 흡수 및 신세뇨관 수송에 미치는 영향을 검토하고자 rat소장흡수와 가토에서 furosemide의 정맥주사 후 혈중, 요중농도 및 이뇨효과에 미치는 영향을 검토하였다. 1) furosemide in situ법에 의한 rat 소장 흡수실험에서 PAS 및 PAH의 병용투여 결과 furosemide의 흡수율을 각각 유의있게 감소시켰다. 2) 가토에서 PAS 및 PAH의 병용투여 결과 furosemide의 혈중농도는 furosemide 단독투여군에 비하여 증가하였으며, PAH 병용투여군은 유의성을 나타냈으나 PAS 병용투여군은 유의성이 없었다. 3) furosemide의 요중배설량은 PAS 및 PAH의 병용투여 결과 furosemide 단독투여군에 비하여 각각 유의성 있는 억제를 나타냈다. 4) 요�U, Na^+과 Cl^- 배설량 및 osmolal clearance는 PAS 및 PAH의 병용투여시 furosemide 단독투여군에 비하여 유의한 배설억제를 나타냈으나 K^+ 배설량은 별 영향이 없었다. 5) Na^+ 재흡수율은 PAS 및 PAH 병용투여에 의해 furosemide 단독투여에 비하여 각각 유의한 증가를 나타냈다. 6) urine pH와 GFR은 별 영향이 없었다.

유기산성약물이 푸로세미드의 흡수, 배설및 이뇨효과에 미치는 영향

염윤희,용재익 대한약학회 1987 약학회지 Vol.31 No.5

The effects of organic acidic drugs on the absorption, excretion and diuretic action of furosemide were studied. Cefalexin, p-aminohippuric acid (PAH), ibuprofen and p-amino salicylic acid (PAS) were selected as organic acidic drugs. The in situ absorption rate and absorption rate constant of furosemide (30$\mu{M}$) were significantly (p<0.05) decreased by 30$\mu{M}$ of cefalexin, PAH, ibuprofen and PAS in rat small intestine. The plasma concentration of furosemide was significantly (p<0.01) increased by cefalexin, PAH and ibuprofen in rabbits. But the urinary excretion rate, renal clearance and diuretic action of furosemide were significantly (p<0.05) decreased by cefalexin, PAH, ibuprofen and PAS in rabbits.

Effect of Acetazolamide on the Diuretic Action of Furosemide in Rabbits

염윤희,이종화,김인순,김옥녀,조규철,Yeom, Yoon-Hee,Lee, Jong-Hwa,Kim, In-Soon,Kim, Ock-Nyu,Cho, Kyu-Chul The Korean Society of Pharmacology 1981 대한약리학잡지 Vol.17 No.2

Furosemide의 이뇨작용에 대한 acetazolamide의 영향을 알아보고자 토끼를 4군으로 나누어 saline(0.5ml/kg), acetazolamide (10mg/kg), furosemide (0.5mg/kg), acetazolamide (10mg/kg)+furosemide(0.5mg/kg)를 각각 정맥주사하였다. 1) Acetazolamide와 furosemide 병합투여시 furosemide를 단독투여했을 때의 뇨량, 뇨중 전해질 배설량에 비하여 유의한 감소를 보였다. 2) Acetazolamide와 furosemide 병합투여시와 furosemide를 단독투여시의 뇨중 전해질과 뇨량의 fraxtional excretion rate를 비교하면, 병합투여시에 감소되어 나타났으며 뇨중전해질보다 뇨량에서 그 정도가 심하였다. 3) Acetazolamide와 furosemide 병합투여시 뇨 pH가 furosemide 단독투여시보다 높게 나타났다. 4) 뇨중 $Cl^-$배설량에 대한 $Na^+\;+K^+$배설량의 비는 acetazolamide + furosemide 병합투여군과 furosemide 단독투여군에서 유의한 차이를 보이지 않았다. Acetazolamide와의 병합투여로 나타나는 furosemide 이뇨작용의 감소는 뇨 pH의 증가 또는 ascending Henle‘s limb에서의 $Cl^-$재흡수 억제의 감소에 기인하는 것으로 사료된다. Effect of acetazolamide on the diuretic action of furosemide was investigated in rabbits. The rates of urine flow and excretion of salts were significantly reduced when furosemide (0.5 mg/kg) was administered with acetazolamide (10 mg/kg) compared to the diuretic response of the single furosemide (0. 5 mg/kg) administration. Reduction in the fractional excretion rate of urine volume was more pronounced than the fractional excretion rate of salts. The results suggest that reduction of diuretic action on furosemide by combined administration of acetazolamide is probably due elevated urinary pH and interference in the mechanism of inhibition of chloride transport in the ascending Henle's limb.

흰쥐에서 기원한 전립선 상피세포인 NRP-152에서 Tamsulosin에 의해 유도되는 세포고사와 Clusterin의 발현에 관한 연구

염윤희,유탁근,윤용달,우제형 대한배뇨장애요실금학회 2006 International Neurourology Journal Vol.10 No.2

Purpose: The aim of this study was to know whether and how tamsulosin induces apoptosis of normal rat prostate cells, and the relationship between apoptosis and clusterin expression. Materials and Methods: We used a prostate cell line, NRP-152 cells which are the basal epithelium cell originated from rat prostate. The NRP-152 cells were treated with various concentrations (50, 100, 200, 400 uM) of tamsulosin for 24 h. To evaluate apoptosis, the cultured NRP-152 cells were stained with Heochst 33258 and Propidium Iodide (PI) without fixation. We also examined DNA fragmentation analysis to confirm apoptosis. In addition, to elucidate the signal transduction pathway by which apoptosis is induced, we examined Bcl-2 family proteins such as Bcl-2, Bax, Bad, Bcl-xL, and Bim by real-time RT-PCR. Results: After tamsulosin treatment, the rate of apoptosis was 25% at 100μM, 50% at 200μM, and 63% at 400μM, whereas the rate of necrosis was 10% at 100μM, 38% at 200μM, and 56% at 400μM. DNA fragmentation was also gradually increased and the highest at 400μM, similar to apoptotic cell rates. As a result of real-time RT-PCR, there was significant difference of Bcl-2 and Bim mRNA expression among the groups. Expression of clusterin protein was significantly increased after treatment of tamsulosin, even as low as 50μM concentration. Conclusion: These results demonstrate that tamsulosin causes the cell death of NRP-152 cells, displaying low concentration of tamsulosin induces apoptosis, but high concentration occurs necrosis. Bim, a proapoptotic factor of the Bcl-2 family, expression was increased in the cells treated with tamsulosin, whereas Bcl-2 expression was decreased. The present study suggests that clusterin may play a role in the process of apoptosis induced by tamsulosin and Bim could be involved in the apoptosis. (J. Korean Continence Society 2006;10:132-139)

Hydrocortisone이 Furosemide의 신세뇨관 수송에 미치는 영향

염윤희,용재익,이종화,조규철 한림대학교의료원 1983 人間科學 Vol.7 No.1

사람 유방암 세포주 MCF-7에서 Tamoxifen이 세포사멸과 세포증식에 미치는 영향

염윤희,김상수,양현원,윤용달 한국발생생물학회 2003 한국발생생물학회 학술발표대회 Vol.2003 No.1

GnRH-agonist에 의한 인간 과립-황체화 세포의 세포사멸과 PBR 단백질의 발현

김세광,염윤희,윤정미,배상욱,양현원,조동제,윤용달,송찬호,Kim, Sei-Kwang,Youm, Yun-Hee,Yoon, Jeong-Mi,Bai, Sang-Wook,Yang, Hyun-Won,Cho, Dong-Jae,Yoon, Yong-Dal,Song, Chan-Ho 대한생식의학회 2004 Clinical and Experimental Reproductive Medicine Vol.31 No.2

Objective: To investigate whether GnRH-agonist (GnRH-Ag) using in IVF-ET affects apoptosis of human granulosa-luteal cells and expression of peripheral benzodiazepine receptor (PBR) protein involved in the apoptosis of the cells. Methods: Granulosa-luteal cells obtained during oocyte retrieval were cultured and treated with $10^{-5}M$ GnRH-Ag. Apoptosis of the cells by the treatment was confirmed using DNA fragmentation analysis 24 h after culture. The presence of PBR protein within the cells was examined by immunofluorescence staining and the expression of the protein was analyzed by Western blotting. In addition, it was measured for progesterone and nitric oxide (NO) produced by granulosa-luteal cells after GnRH-Ag treatment. To evaluate the relationship between NO production and PBR expression, sodium nitroprusside (SNP) as a NO donor was added in media and investigated the expression of PBR protein by Western blotting. Results: Apoptosis increased in the granulosa-luteal cells 24 h after GnRH-Ag treatment, whereas the expression of PBR protein significantly decreased. Furthermore, the production of progesterone and nitric oxide (NO) by the cells significantly fell from 12 h after the treatment. In the results of Western blotting after SNP treatment, the expression of PBR protein increased in the treatment with SNP alone to the granulosa-luteal cells, but was suppressed in the treatment with GnRH-Ag and SNP. Additionally, the staining result of PBR protein in the cells showed the even distribution of it through the cell. Conclusion: These results demonstrate that GnRH-Ag treatment induces apoptosis, decreasing expression of PBR protein and NO production in human granulosa-luteal cells. The present study suggests that one of the apoptosis mechanism of human granulosa-luteal cells by GnRH-Ag might be a signal transduction pathway via NO and PBR.

인간 피부조직에서 멜라닌 줄기세포 확인 및 분리방법에 관한 연구

김난형,염윤희,최애진,양현원,이애영 한국발생생물학회 2003 한국발생생물학회 학술발표대회 Vol.2003 No.1