습사료에 첨가한 유용미생물 및 한약재 혼합제(한방천ㆍ어력천) 특성과 혼합 첨가제가 넙치간의 활성에 미치는 효과

여인규,노섬,Yeo, In-Kyu,Rho, Sum 한국양식학회 2004 韓國養殖學會誌 Vol.17 No.2

유용 미생물과 한약재가 포함된 새로운 사료첨가제의 다양한 농도(0.3, 0.6 및 0.9%)에 따른 넙치의 간기능의 활성에 미치는 영향 및 사료첨가제의 특성에 대해서도 조사하였다. 첨가제에 포함된 유용미생물(유산균, 바실러스균, 효모 및 광합성균)의 총수는 한방천이 5.6${\times}$$10^{8}$ CFU/g이었으며, 어력천은 3.0${\times}$$10^{8}$ CFU/g 이었다. 앙식에 일반적으로 사용되는 습사료의 병원미생물(Edwardsiella tarda, Vibrio anguillarum and Streptorwccus sp.)은 모든 첨가군에 있어서 첨가제의 농도의존적으로 유의한 감소를 나타내었다. 간중량 지수는 0.3%의 첨가군에서 유의하게 증가하였다. 혈중의 단백질량은 모든 첨가군이 유의한 증가를 나타내었으나. 간장내의 단백질량은 대조군이 높은 수치를 나타내었다. 생체방어기작을 하는 catalase 및 superoxide dismutase는 0.3% 및 0.6% 첨가군에서 각각 높은 활성을 나타내었다. 이상의 결과로 한약재와 유용미생물 혼합 사료첨가제의 사용으로 넙치의 항산화효소의 활성을 증대시켜 어류스트레스의 면역증대를 기대할 수 있을 것으로 여겨진다. The effects of different concentrations (0, 0.3, 0.6 and 0.9%) of fish feed additives (Hanbangchun and Olyukchun) utilizing effective microorganisms and herb medicine on activity of liver function were examined in olive flounder, Paralichthys olivaceus, Moreover, we investigated the characteristics of the additives. Total number of microorganisms (Lactic acid bacteria, Bacillus subtilis, Saccharomyces cerevisiae, Photosynthetic bacteria and Azotobactor) in the additives was 5.6${\times}$10$^{8}$ CFU/g in the Hanbangchun and 3.0${\times}$10$^{8}$ CFU/g in the Olyukchun. Levels of three typical pathological microorgamisms (Edwardsiella tarda, Vibrio anguillarum and Streptococcus sp.) in moist pellets (MP) were significantly decreased by the additives in a concentration-dependent way. Hepatosomatic index of fish in the 0.3% group was significantly increased. Total serum protein was increased in all the groups containing additives, but the protein content in liver was higher in the control group. Higher activities of catalase and superoxide dismutase which are involved in physiological defense mechanisms were found in the dietary groups containing 0.3% and 0.6%, respectively. These results suggest that the additives, Hanbangchun and Olyukchun, can increase tolerance of olive flounder against stress and hypoxic conditions by increasing activities of body antioxidant enzymes.

넙치 Paralichthys olivaceus 초대 배양 간세포의 난황 전구물질 합성에 미치는 estradiol-$17{\beta}$와 2,4-D의 영향

여인규,최미경,이영돈,임윤규,허문수,이제희,송춘복,Yeo, In-Kyu,Choe, Mi-Kyung,Lee, Young-Don,Lim, Yoon-Kyu,Heo, Moon-Soo,Lee, Je-Hee,Song, Choon-Bok 한국어류학회 2000 韓國魚類學會誌 Vol.12 No.3

Estradiol-$17{\beta}(E_2)$와 2,4-dichlorophenoxy acetic acid (2, 4- D)가 난황 전구물질의 합성에 미치는 영향을 넙치 Paralichthys olivaceus 간세포의 초대 배양을 통하여 조사하였다. 배양간세포의 생존율은 배양온도 $27^{\circ}C$에 서 가장 높게 나타났으며, $15^{\circ}C$에서는 생존율이 급격히 감소하여 약 50%의 생존율을 나타내었다. $E_2$에 의한 VTG 의 합성은 $10^{-6}M$에서 최대치를 나타내었다. 2, 4-D $10^{-7}\sim10^{-5}M$의 첨가에 의해 VTG의 합성은 이루어지지 않았다. 그러나, 저농도인 $10^{-8}M$에서는 VTG의 합성이 증가하였다. $E_2$ 및 2, 4-D에 의해 합성된 VTG는 $10^{-6}M$ tamoxifen의 첨가에 의해 유의하게 억제되었다 (P<0.01). 본 연구 결과에서 $E_2$와 2, 4-D의 동시 첨가는 VTG의 합성을 억제하지 않았다. 이러한 결과는 2, 4-D의 작용이 $E_2$와 유사한 작용을 가지지만, VTG의 합성에 있어서 $E_2$ 수용체에의 작용 양식은 서로 다른 것으로 추정된다. Effects of Estradiol-$17\beta(E_2)$ and 2, 4-dichlorophenoxy acetic acid (2, 4-D) on vitellogenin(VTG) production were investigated in primary hepatocyte culture of olive flounder, Paralichthys olivaceus. Highest survival rate of hepatocyte were observed at $27^{\circ}C$, which markedly declined equal to 50% of those of $15^{\circ}C$. Vitellogenin production peaked at the concentration of $10^{-6}M\;E_2$. No effect was observed on VTG production at various concentrations of 2, 4-D. However, a low concentration of 2, 4-D (ie, $10^{-8}M$) only appeared increased VTG production. $E_2$ or $10^{-8}M$ 2, 4-D-primed VTG production was markedly inhibited by the addition of $10^{-6}M$ tamoxifen to the culture medium(P<0.01). Inhibition was not affected by combinational treatment with $10^{-6}M$ $E_2$ and $10^{-6}M$ 2, 4-D. These results from the current investigation suggest that 2, 4-D mimics $E_2$, but the mechanism of reaction in inducing the $E_2$ receptor are different in VTG production in oliver flounder hepatocytes.

레이저 피닝 시뮬레이션을 통한 금속재료의 물성치 예측

여인규,임현택,임종빈,정성호,Yeo, In Kyu,Lim, Hyun Taek,Lim, Jong Bin,Jeong, Sung Ho 한국레이저가공학회 2013 한국레이저가공학회지 Vol.16 No.1

For numerical simulation of the deformation and residual stress development in metals during laser peening, the constitutive equations describing material behavior at various strain rates and plastic properties at high strain rate impact conditions are prerequisite. However, these parameters are often unknown for various engineering materials. In this study, the parameters of Johnson-Cook constitutive equation of duplex stainless steel were determined by comparing the residual stress profile predicted by numerical simulation using trial values and the measured residual stress profile with x-ray diffraction.

감귤박 및 감태추출물의 사료첨가제 급여에 따른 둥근전복 (Haliotis discus discus)의 성장 및 생리적 변화

좌민석,여인규,Jwa, Min-Seok,Yeo, In-Kyu 한국어병학회 2015 한국어병학회지 Vol.28 No.1

Here, we report the physiological changes and growth in disk abalone, Haliotis discus discus, in relation to dietary supplementation with citrus pomace (CP) 6%, Ecklonia cava residue (ECR) 6%, and CP + ECR (3% + 3%). The composition and nutrient content, survival rate and growth rate were measured 0, 4, 8 and 12 weeks after feeding the supplemented diets of CP and/or ECR. Moreover, the experiment of low salinity stress (25psu) for environmental resistance was examined for a period of 48 hours after feeding the supplemented diets for 12 weeks. The activities of superoxide dismutase (SOD), catalase (CAT), lysozymes, respiratory burst, and phenoloxidase were measured. The moisture content and crude protein condition of the body were increased with the addition of ECR only (P<0.05). We observed higher levels of survival in the experimental group compared with the control group. Moreover, the growth disk abalone that were fed a diet containing ECR was higher compared with the control group. However, the growth of abalone fed a diet containing CP was similar to the control group. With a rearing condition of low salinity stress, survival rate and lysozyme activity were increased in the ECR group compared with the control group. Dietary ECR reduced the level of CAT activity to approximately 30% of the control, however the level of CAT activity in the ECR group was similar to the start level of the previous stress. These results suggest that dietary ECR gives rise to an enhanced immunity in disk abalone, as a result of the decrease in CAT and lysozyme activity in particular. Accordingly, the growth and survival rate were increased by feeding an ECR-supplemented diet in the rearing of disk abalone, Haliotis discus discus.

감귤박 및 감태추출물의 사료첨가제 급여에 따른 둥근전복 (Haliotis discus discus)의 성장 및 생리적 변화

좌민석 ( Min Seok Jwa ),여인규 ( In Kyu Yeo ) 한국어병학회 2014 한국어병학회지 Vol.28 No.1

Here, we report the physiological changes and growth in disk abalone, Haliotis discus discus, in relation to dietary supplementation with citrus pomace (CP) 6%, Ecklonia cava residue (ECR) 6%, and CP + ECR (3% + 3%). The composition and nutrient content, survival rate and growth rate were measured 0, 4, 8 and 12 weeks after feeding the supplemented diets of CP and/or ECR. Moreover, the experiment of low salinity stress (25psu) for environmental resistance was examined for a period of 48 hours after feeding the supplemented diets for 12 weeks. The activities of superoxide dismutase (SOD), catalase (CAT), lysozymes, respiratory burst, and phenoloxidase were measured. The moisture content and crude protein condition of the body were increased with the addition of ECR only (P<0.05). We observed higher levels of survival in the experimental group compared with the control group. Moreover, the growth disk abalone that were fed a diet containing ECR was higher compared with the control group. However, the growth of abalone fed a diet containing CP was similar to the control group. With a rearing condition of low salinity stress, survival rate and lysozyme activity were increased in the ECR group compared with the control group. Dietary ECR reduced the level of CAT activity to approximately 30% of the control, however the level of CAT activity in the ECR group was similar to the start level of the previous stress. These results suggest that dietary ECR gives rise to an enhanced immunity in disk abalone, as a result of the decrease in CAT and lysozyme activity in particular. Accordingly, the growth and survival rate were increased by feeding an ECR-supplemented diet in the rearing of disk abalone, Haliotis discus discus.

사육수의 pH변화가 복해마(Hippocampus kuda)에 미치는 생리적 영향

박천만(Cheonman Park),김기혁(Ki-hyuk Kim),문혜나(Hye-Na Moon),여인규(In-Kyu Yeo) 한국생명과학회 2017 생명과학회지 Vol.27 No.7

대기의 이산화탄소의 농도 증가는 해양산성화와 지구온난화를 유발하는 것으로 알려져 있다. 해마는 해양생태계 및 수산자원생물로서 중요한 종으로 알려져 있지만, 최근 해양산성화로 인하여 개체수가 감소되고 있는 실정이다. 따라서 본 연구에서는 멸종 위기 종인 복해마(Hippocampus kuda)에 미치는 생리적 영향을 조사하기 위해서 사육수의 산성조건인 pH 6.0, 6.5, 7.0 및 자연해수(pH 8.0)의 환경에서 복해마(H. kuda)를 15일 동안 사육 후 체내조성 변화 및 항산화 효소 활성 변화에 대하여 조사를 실시하였다. 복해마(H. kuda)의 크기 및 성장은 대조군인 pH 8.0을 제외한 실험군에서는 pH가 저하함에 따라 감소하는 경향을 나타내었다. 체내 조성성분인 회분, 조지방 및 조단백 또한 pH 저하에 따라 농도의존적으로 감소하는 것이 관찰되었다. SOD, CAT 및 GSH와 같은 항산화 효소의 분석 결과, SOD활성의 경우, pH 저하에 따라 농도의존적으로 감소하지만, 이와 상반되게 CAT 및 GSH에서는 pH저하에 따라 활성이 농도의존적으로 증가하는 결과가 나타내었다. 이것은 복해마(H. kuda)가 사육수의 pH 저하에 따른 체내 항상성을 유지하는 과정 중 스트레스가 야기되어 에너지 대사가 손상된 것으로 추정된다. 항산화효소는 일반적으로 산성화 스트레스에 민감하게 작용하는데 본 연구에서도 사육수의 pH 변화에 따라 항산화 효소작용이 유의하게 변화하였다. 이러한 결과로 복해마(H. kuda)에 있어서 산성화 노출을 통한 생리학적 스트레스가 항산화 반응 및 체내 성분과 성장을 저해하는 것으로 여겨진다. The rising concentration of atmospheric carbon dioxide is causing ocean acidification and global warming. The seahorse is an important species in marine ecosystems and fishery markets, however, their populations have recently decreased due to ocean acidification. As a result, we examined changes in the physiological responses of the spotted seahorse Hippocampus kuda when it was exposed to acidic sea water (pH 6.0, 6.5, and 7.0) and normal seawater (pH 8.0 as the control) over a period of 15 days. As the pH decreased, the seahorses’ body weight and length also decreased. Components in body of ash, the crude lipids and crude proteins also differed significantly with changes in pH, due to stress caused by the seahorses’ exposure to the acidic water conditions. The superoxide dismutase levels were significantly lower in the pH 6.0 and 6.5 groups than they were in the pH 7.0 and pH 8.0 groups. However, the catalase and glutathione levels were significantly higher in the acidic sea water groups. We suggest that decreasing the pH level of rearing water induces a stress response in H. kuda, damaging their ability to maintain their homeostasis and energy metabolism. Antioxidant enzymes are generally sensitive to acidic stress; in this study, the antioxidant activity was significantly affected by the pH level of the rearing water. These results indicate that physiological stress, induced by exposure to acidification, induces an antioxidant reaction, which can reduce general components in the body and the growth of H. kuda.

여윔증 넙치, Paralichthys olivaceus의 증상에 대한 병태생리학적 고찰

김이경 ( Yi Kyung Kim ),정준범 ( Joon Bum Jeong ),이무근 ( Mu Kun Lee ),박수일 ( Soo Il Park ),박명애 ( Myeong Ae Park ),최미경 ( Mi Kyung Choe ),여인규 ( In Kyu Yeo ) 한국어병학회 2011 한국어병학회지 Vol.24 No.1

This study was aimed to investigate the pathophysiological changes of olive flounder, Paralichthys olivaceus suffering from emaciation. A plasma osmolality was higher in the emaciated and control flounders than that of normal teleost, suggesting osmoregulatory failure in both of them. Also, the control in the same stock with emaciated flounder seem to be classified into a primary degree of emaciation. According to microscopic observations, the inflammatory responses were observed in the submucosal layer of anterior intestine, although the some of mucosal intestinal epithelium still remained. It was suggested that the pathological changes of the anterior part give rise to malabsorption of nutrients through the mucosa. In the posterior intestine and rectum, the mucosal epithelium were almostly sloughed off and severe inflammatory responses were observed in the submucosa. Immunoreaction for NKCC was not detected in the mucosal epithelial cells in intestine because of sloughing of epithelium. These changes would lead to functional disorder in the intestine, such as malabsorption of nutrients and osmoregulatory failure. Also important is to investigate the recovery phase.

사료 내 마늘 추출액 첨가가 넙치(Paralichthys olivaceus)의 비특이적 면역반응 및 질병저항성에 미치는 영향

김승민 ( Seung Min Kim ),전려진 ( Lyu Jin Jun ),여인규 ( In Kyu Yeo ),전유진 ( You Jin Jeon ),이경준 ( Kyeong Jun Lee ),정현도 ( Hyun Do Jeong ),정준범 ( Joon Bum Jeong ) 한국어병학회 2014 한국어병학회지 Vol.27 No.1

In this study, we investigated the effects of dietary 1% garlic extract on nonspecific immune responses and fish diseases (Viral Hemorrhagic Septicemia Virus, Vibrio anguillarum, Streptococcus iniae and Edwardsiella tarda) resistance in olive flounder Paralichthys olivaceus. Fish were fed a commercial diets supplemented with 1% garlic extract for 4 weeks. After the 4 weeks feeding experiment, the artificial infection was made by V. anguillarum, S. iniae, E. tarda and VHSV. And the cumulative mortality was monitored for 2 weeks after artificial infection. The cumulative mortalities decreased in all experiments except for group of E. tarda compared to control group. We observed significantly higher levels of the hematocrit, glucose, total protein, lysozyme activity and the macrophage activity in the experimental group compared to the control group. In the experiments of drug sensitivity and MIC using the three bacteria (V. anguillarum, S. iniae and E. tarda), 1% garlic extract was more effective than the previously reported fermented garlic powder. These results suggested that garlic extract can increase the disease resistance of olive flounder against V. anguillarum, S. iniae and VHSV and the ability of nonspecific immune responses.

습사료에 첨가한 유용미생물 및 한약재 혼합제(한방천ㆍ어력천)의 특성과 혼합 첨가제가 넙치 간의 활성에 미치는 효과

여인규(In-Kyu Yeo),노섬(Sum Rho) 한국수산과학회 양식분과 2004 韓國養殖學會誌 Vol.17 No.2

사료공급 횟수가 방류용 연어(Oncorhynchus keta) 치어 성장에 미치는 영향

여인규(In-Kyu Yeo),최미경(Mi-Kyung Choe) 한국수산과학회 양식분과 1998 韓國養殖學會誌 Vol.11 No.3