단문 : 한국에서 분리한 Bacillus thuringiensis 균주의 cry형 유전자의 동정

박수일 ( Soo Il Park ),이광용 ( Kwang Yong Lee ),강은영 ( Eun Young Kang ),김의나 ( Eui Na Kim ),권혁한 ( Hyuk Han Kwon ),안성규 ( Seong Kyu Ahn ),이형화 ( Hyung Hoan Lee ) 한국미생물생명공학회 2005 한국미생물·생명공학회지 Vol.33 No.2

수온이 조피볼락에 기생하는 아가미흡충의 산란과 부화에 미치는 영향

박수일(Soo Il Park),김기홍(Ki Hong Kim),최은석(Eun Seok Choi),황윤정(Yoon Jung Hwang),조재범(Jae Bum Cho) 한국어병학회 1998 한국어병학회지 Vol.11 No.2

The influence of temperature on the rate of egg production and embryonic development of Microcotyle sebastis was investigated to determine the precise time of a second treatment. The survival time of the adults of M. sebastis was inversely proportional to temperature. The number of laid eggs per each replicate during the first 24 h was 39.3±4.0 at 10℃, 62.7±14.2 at 15℃, 101.0±5.6 at 20℃ and 89.0±11.0 at 25℃. The time required for egg hatching of M. sebastis was 31.30±4.88, 17.52±3.24, 11.59±3.02 and 10.76±3.10 days at 10, 15, 20 and 25℃, respectively. The regression models of the time required for the beginning and 50% point of hatching according to the different temperatures were as follows; Beginning of hatch: D = 58.2000 - 4.2067 × Temp+0.0867 x (Temp)² (P≤0.01), 50% of hatch: D = 91.3833 - 7.5767 × Temp+ 0.1767 × (Temp)² (P≤0.01).

대합 ( Meretrix lusoria Roding ) 의 간췌장에 기생하는 Rickettsia - like organisms 에 대한 연구

박수일(Soo Il Park),조미영(Mi Young Cho),송재희(Jae Hee Song),양원석(Won Seok Yang) 한국어병학회 2001 한국어병학회지 Vol.14 No.3

The histopathological survey was performed to search for the cause of mass mortality of the hard clam Meretris lusoria in 1999. Histological section showed spherical or elongated intracellular basophilic inclusions in the gill filaments and digestive diverticula of the moribund clams. The hypertrophy and lysis of epithelial cells and multifocal necrosis were observed in affected tissues. TEM demonctrated two different rickettsia-like organisms; the one was large, coccoidal (1.2~2.0㎛ in diamenter) organisms observed in the nera nuclei and the other was polymorphic, rod-shaped (0.27~0.58×1.18㎛)organisms tightly packed within cytoplasmic vacuoles or scattered throughout the cytoplasms. Moreover, the smallest, mineralized, cell walled nanobacterias (146~600×261~625nm)were found in various tissues of the moribund hard clams. In nanobacteria-infected tissues cytoplasmic vacuolization and numerous crystalline deposits were found.

인체이동에 관한 운동역학적 분석 1

박수일 ( Sue Il Park ),변범수 ( Beom Soo Beun ) 한국운동역학회 1994 한국운동역학회지 Vol.4 No.2

During walking, jogging and sprinting, the support time, stride time of both legs and maximal ground reaction forces was investigated using dynamic strain amplifier and repicorder with strain gages for six male college students(3 nonathletes:3 athletes) in the department of physical education. The results this study seem to be the followings: 1. The greater the speed, the more the support time, nonsupport time and stride time was decreased. But it was shown that the nonsupport time during jogging was longer rather than one during walking. 2. Difference between the support time and nonsupport time of right and left leg shown a significant relationship during walking and jogging. whereas it was not shown significantly during sprinting in most subjects. 3. Maximal reaction forces of heel and forefoot were 1.34 BW: 1.38 BW(heel : forefoot) during walking and 1.32 BW: 2.04 BW during jogging and also were 2.49 BW(forefoot) during sprinting, respectively. 4. The % time of maximal ground reaction forces of heel and forefoot were 19.44% time : 79.83% time(heel : forefoot) during walking and 9.96% time : 43.92% time during jogging and also 53.70% time(forefoot) during sprinting.

필터의 개발을 통한 해수 육상수조식 양식장의 환경개선에 관한 연구 II. 필터 운행에 따른 유입수 및 배출수의 정화효과

강주찬,박수일,김성근,KANG Ju-Chan,PARK Soo-Il,KIM Seoung-Gun 한국수산과학회 1998 한국수산과학회지 Vol.31 No.6

This study was conducted to evaluate the purification efficacy by filter and ultra violet (UV) irradiation against inlet and outlet seawater of the land based fish farm. Purification efficacies for inlet seawater (50$\ell$/min) has been examined with filter of 60$\mu$m pore size and UV irradiation at an average dose 0.5 mWS/$cm^2$ for 5 months. For outlet seawater filter of 90 $\mu$m pore size only was used. Temperature, salinity, pH, $NO_3-N,\;NO_2-N,\;NH_4-N,\;PO_4-P$, DO and COD of inlet and outlet seawater in the system were not influenced. However, the removing rate of suspended solid and turbidity of inlet seawater were appeared to be $48.7\~65.6\%$ (average $51.9\%$) and $33/3\~42/5\%$ (average $34.8\%$) after passing through screen filter respectively. Also, germicidal efficiency to the total bacteris and Vibrio species were $16.7\~20.2\%$ (average $19.2\%$) $20.0\~21.9\%$ (average $20.9\%$) respectively after passing through UV irradiation. After passing through drum filter of 90$\mu$m pore size, suspended solid and turbidity of outlet seawater were appeared to be $42.7\~52.6\%$ (average $46.9\%$), $27.7\~29.9\%$ (average $28.3\%$), respectively.

필터의 개발을 통한 해수 육상수조식 양식장의 환경개선에 관한 연구 III. 스크린필터 및 자외선 등의 운행에 따른 사육수의 정화효과

강주찬,박수일,김성근,KANG Ju-Chan,PARK Soo-Il,KIM Seoung-Gun 한국수산과학회 1999 한국수산과학회지 Vol.32 No.4

This study was conducted to evaluate the purification efficiency in rearing water of the land based fish farm by screen filter and ultra violet (UV) irradiation. Purification efficiency for rearing seawater has been examined with screen filter of 60 $\mu$m pore size and UV irradiation at dose of 0.5 $mWS/cm^2$ for 5 months. Purification efficiency by changing of temperature, salinity, pH, DO, total bacteria and Vibrio species in rearing seawater by filtering and UV irradiation were not significant during 5 months, However, the removing rate of suspended solid and turbidity of rearing seawater were $43.8\~45.6\%$ (average, $44,7\%$) and $29.2\~33.2\%$ (average, $31,3\%$) by filtering, respectively. Also, Purification efficiency for the $NO_3^{-}-N,\;NO_2^{-}-N,\;NH_4^{+}-N$ and $PO_4^{3-}-P$ were $21.3\~21.9\%$ (average, $21.6\%$), $24.1\~25.2\%$ (average, $24.7\%$), $17.6\~17.8\%$ (average, $17.7\%$) and $19.0\~20.4\%$ (average, $19.7\%$) respectively by the system used on this study.

면역효소항체법에 의한 $\beta$-용혈성 연쇄구균 Streptococcus sp.의 신속진단에 대하여

전세규,최동림,박수일,Chun, Seh-Kyu,Choi, Dong-Lim,Park, Soo-Il 한국어병학회 1988 한국어병학회지 Vol.1 No.2

For the rapid diagnosis of bacterial diseases of cultured fishes, the immunoperoxidase method was applied to the detection of $\beta$-haemolitic Streptococcus sp. strain KST-2 isolated from tilapia(Oreochromis niloticus). The suitability of field analysis and the sensitivity of the immunoperoxidase method was compared with those of the counterimmunoelectrophoresis(CIE) and the immunodiffusion(ID). Results of testing cross-reactivity which did not indicate any cross reactivity with other fish pathogens, this method was specific to Streptococcus sp. The sensitivity of this method was $1{\times}10^3CFU/ml$ which was at least $10^2$times greater than the CIE and $10^4$times greater than the ID. The immunoperoxidase method was more suitable for field application and more sensitive than other diagnostic techniques tested on this study. 양식어류의 세균성 질병의 신속한 진단을 위하여 틸라피아(O. niloticus)로부터 분리된 $\beta$용혈성 연쇄구균인 Streptococcus sp. KST-2균주를 사용하여 면역효소법으로 검출실험을 하였으며, 면역효소법의 현장사용의 용이함과 검출의 민감도를 알아보기 위하여 counterimmunoelectrophoresis(CIE), 및 이중면역확산법등과 비교하여 보았다. 다른 어류 병원균과의 교차반응 결과 교차반응이 전혀 일어나지 않는 것으로 보아 이 방법은 $\beta$용혈성 연쇄구균(KST-2균주)에 매우 특이적임을 알 수 있었다. 연쇄구균의 검출실험한 결과 면역효소법은 $1{\times}10^3CFU/ml$의 농도까지 검출가능하였으며, 검출의 민감성이 CIE에 비해 $10^2$배, 이중면역확산법에 비해 $10^4$배나 높은 것으로 나타났다. 면역효소법은 이번 연구에서 사용된 진단법 중에서 현장사용이 가장 용이하며, 민감성이 가장 뛰어난 것으로 나타났다.

담수산 백점충(Ichthyophthirius multifiliis)에 관한 연구 II. 백점충의 인위 감염 및 어체내 충체 발달상

지보영,김기홍,박수일,김이청,Ji, Bo-Young,Kim, Ki-Hong,Park, Soo-Il,Kim, Yi-Cheong 한국어병학회 1998 한국어병학회지 Vol.11 No.1

Concerned to the lyfe cycle of Ichthyophthirius multifiliis, the experimental infection and development of the parasites were studied in the several freshwater cultured fishes. Opitimum conditions for the propagation of the parasite by serial passage with the rainbow trout fry was observed. Visiable white spots were examined in the body surface, fins and gills of the healthy fries, and a stable infection has been maintained for 2 months in the experimental system (Temperature: $18{\pm}1^{\circ}C$ DO: 7-7.5 ppm; pH: $7{\pm}0.2$). Induction conditions for artificial infection of the parasite by interms of the host fishes, stages of the parasites, and rearing temperature regimes was investigated. Rainbow trout fries showed a positive infection which was resulted from exposure of theront at $18^{\circ}C$. The rainbow trout fries induced white spots on the body surface at 3-7 days exposure to the theronts at $18^{\circ}C$. It was found that exposure of the rainbow trout fries exposed to 1,000 theronts per fish (10 theront/ml) for 45-60 minutes at $18^{\circ}C$ would consistently produce infection. Perfect infection (100%) was induced when the fries were exposed to 1,500 theront per fish (15 theront/ml) under laboratory condition. Development of I. multifiliis in the rainbow trout was observed for 7 days postexposure (PE). The parasite increased in average diameter from $54{\mu}m$ on the 1st day PE to $426{\mu}m$ on the 7th day PE. In the initial infestation period, the parasites were found on the gill epithelium, and on the 3rd day PE they invaded into the basal part of the gill filament adjacent to the major blood vessels, particularly the afferent vessels. Morphological change of buccal apparatus were observed on the 2nd day PE. Contractile vacuoles were more prominent on the 4th day PE, and they had notable changes on the 7th day PE.

필터의 개발을 통한 해수 육상수조식 양식장의 환경개선에 관한 연구 I. 스크린 및 드럼 필터의 개발

김성근,강주찬,박수일,KIM Seoung-Gun,KANG Ju-Chan,PARK Soo-Il 한국수산과학회 1998 한국수산과학회지 Vol.31 No.6

The productivity of land based seawater fish farm has been decreased because of unexpected outbreaks of diseases caused by the contaminated inlet seawater. Sometimes unfiltered/untreated outlet seawater from the land based seawater fish farm has created serious environmental problem. In the needs of treatment systems for the inlet and outlet seawater, the researchers have developed two different systems, The purpose of this study is to design and test two treatment systems, the screen filter for inlet seawater and drum filter for outlet seawater, on the basis of concept of system design and automatization. After developing two systems, an experiment has been conducted with two systems and collected data to improve design and efficiency of the system. In this study, detailed design and efficiency of the system could be improved by the programmable logic controller (PLC).

틸라피아의 에드와드병에 대한 백신과 Adjuvant 의 효과

이주석(Joo Seok Lee),박수일(Soo Il Park) 한국어병학회 1992 한국어병학회지 Vol.5 No.1

A formalized bacterin(FKC) of Edwardsiella tarda, conjugated with/without Freund`s complete adjuvant(FCA), Freund`s incomplete adjuvant(FIA) and potassium aluminium sulfate(PAS), was administered by intraperitoneal injection to tilapia respectively. The tilapia were reared under indoor recirculating filter system. There were agglutinin titer increment in the tilapia which were administered not only FKC bacterin but also adjuvanted vaccines. But the producing time of the highest level of antibody in the immunized tilapia was different among vaccine preparation, i. e. FKC + FCA group and PAS + FKC group are between the 2nd and the 4th week, and FKC alone group is the 2nd week. In the challenge experiment with 2.5× 10^7 CFU/㎖ or 2.5×10^8 CFU/㎖ of E. tarda T1123 to the tilapia immunized with vaccines, RPS above sixty was recorded both challenge dose in the tilapia which were 3 weeks after immunization with FKC+FCA, PAS+FKC and FKC alone, and the former dose 8 weeks after immunization with FCA+FKC and PAS+FKC. There were some resistance enhancement against E. tarda in the tilapia which were injected with adjuvant alone than that of control. As the results, the FCA or PAS adjuvanted vaccine is effective to sustain the defensible period against edwardsiellosis.