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양웅모,김향미,장문석,박완수,김원남,김산웅,최동기,이학철,김윤경,박성규,Yang, Woong-Mo,Kim, Hyang-Mi,Chang, Mun-Seog,Park, Wan-Su,Kim, Won-Nam,Kim, San-Woong,Choi, Dong-Gi,Lee, Hak-Chul,Kim, Yun-Kyung,Park, Seong-Kyu 대한한의학방제학회 2006 大韓韓醫學方劑學會誌 Vol.14 No.2
The natural plants were screened for new cosmetic materials. Many parts of Nelumbo nucifera has been investigated for anti-wrinkling and anti-furrowing effects. The leaves. flowers and stamens of Nelumbo nucifera were extracted with ethanol or distilled water. The effects of cell viability and $H_2O_2-induced$ cytotoxicity were studied on CCD-986sk human fibroblast cell line by MTT assay. We measured various effects related to skin such as the synthesis of MMP-1 and type I procollagen. The ethanol extract of Nelumbo nucifera leaves (ENL) at concentrations of 30 ${\mu}g/ml$ showed maximum cell viability on CCD-986sk cells by 129.70%. and $H_2O_2-induced$ cytotoxicity was blocked by ENL (5. 10. 20. 30. and 40 ${\mu}g/ml$) concentration-dependently. The synthesis of MMP-1 and type I procollagen were positive in ENL. These results suggest that ENL has anti-oxidant and anti-furrowing effects.
전탕방법에 따른 전탕액의 추출률 비교연구 -평위산 전탕액의 Hesperidin HPLC 분석-
양웅모 ( Woong Mo Yang ),정규진 ( Kyu Jin Chung ),김경욱 ( Kyung Wook Kim ),배현수 ( Hyun Su Bae ),장문석 ( Mun Seog Chang ),박성규 ( Seong Kyu Park ) 대한본초학회 2010 大韓本草學會誌 Vol.25 No.1
Objectives: To evaluate the extraction efficiency of water decoction with or without non-woven fabric underlying comparison of the HPLC (high-performance liquid chromatography) profiles of the hesperidin which is the standard of Citri Pericarpium. Methods: Pyungwisan, which is comprised of Atractylodis Rhizoma, Magnoliae Cortex, Citri Pericarpium, Glycyrrhizae Radix, Zingiberis Rhizoma, and Ziziphy Fructus, was extracted in boiling water with non-woven fabric or without non-woven fabric. Then hesperidin content was analyzed by HPLC. Results: The contents of hesperidin in the water extract of Pyungwisan which was decocted without non-woven fabric showed 219.57±2.51 mg/g and 138.90±6.32 mg/g in with non-woven fabric decoction. Conclusions: These results suggest that herbal decoction without non-woven fabric might increase extraction efficiency.
비파엽추출물의 지방분해효소 조절을 통한 국소 지방분해 효능
이운규,양웅모,최유연 한방비만학회 2017 한방비만학회지 Vol.17 No.2
Objectives: In this study, the lipolytic effects of Eriobotrya folium extract (EFE) on local fat wasinvestigated in high fat diet (HFD)-induced obesity mouse and 3T3-L1 adipocytes. Methods: C57BL/6J mice (5 weeks) were fed HFD for 6 weeks to induce obesity. EFE (20 mg/ml,100 l) or saline (100 l) as a normal control was injected into left inguinal fat pad region, 3 timesper a week for last 2 weeks. After sacrifice, body weight, and histological changes of the inguinalfat pad were evaluated. The expressions of hormone-sensitive lipase (HSL) and adiposetriglyceride lipase (ATGL) in inguinal fat pad were analyzed by Western blotting. Also, lipidaccumulation and lipases release were determined in 3T3-L1 adipocytes by oil red o staining. Results: EFE significantly reduced the weight of inguinal fat pad and the size of adipocytes inHFD-induced obesity mice compared to control. The treatment of EFE up-regulated theexpressions of HSL and ATGL in inguinal fat pads of obesity mice, as well as 3T3-L1 adipocytes. In addition, EFE inhibited the lipid accumulation in 3T3-L1 adipocytes in a dose dependentmanner. Conclusions: EFE showed lipolytic effect on local fat of HFD-induced obesity mice byup-regulation of the lipases secretion. This suggests that EFE could be considered as anti-obesesubstance with lipolytic property on local fat.
급·만성 기관지염과 알레르기성 비염의 국내 의료 이용 현황 분석
정유진,양웅모 대한한의학회 2019 대한한의학회지 Vol.40 No.3
Objectives: Patients with respiratory diseases are increasing as air pollution due to fine dust gets worse. Diseases that occupy a large proportion of respiratory diseases in medical institutions are acute bronchitis, chronic bronchitis and Allergic rhinitis. The number of patients with all three diseases is gradually increasing. This study was to suggest assignment of medicine policy for improving accessibility to Korean medical treatment of respiratory diseases analyzing the current treatment status of acute bronchitis, Chronic bronchitis and Allergic rhinitis patients in Western medical and Korean medical institutes in this situation. Methods: This study used 2017 National patient sample data from the Korean Health Insurance Review and Assessment Service for research. Acute bronchitis, Chronic bronchitis, Allergic rhinitis was according to KCD code. This research contains Socio-demographic analysis classified by sex and age, the number of three disease's patients. Also the number of medical treatment, the expense of recuperation cost, medical practices were analyzed compare with Western and Korean medicine. Results: The incidence of three diseases is higher among female than male. There are many patients under the age of 10 in the case of acute bronchitis and allergic rhinitis, while there are many patients over 50 years of age in chronic bronchitis. Western medical treatment take up a larger proportion than Korean medical treatment in part of the number of three disease's patients, the number of medical treatment, the expense of recuperation cost. Conclusions: Much more patients of these diseases visit in Western medical clinic and hospital than Korean medical clinic and hospital for treatment. There are many parts of Korean medical treatment that are not covered by Health insurance benefits like herbal decoction, pharmacopuncture, etc. Korean medicine need to do the efforts for expanding medical field in variety. Also it is demanded for institutional support for reduction of the cost burden and improving on accessibility of Korean medical treatment in order to treat with the increase in respiratory diseases due to fine dust.
쇄양(鎖陽)의 Diphenyl-picryl-hydrazyl (DPPH) 소거 활성 및 HepG2 세포에 대한 항산화 효과
장문석,양웅모,김도림,박은화,박수연,박성규,Chang, Mun-Seog,Yang, Woong-Mo,Kim, Do-Rim,Park, Eun-Hwa,Park, Soo-Yeon,Park, Seong-Kyu 대한한의학방제학회 2007 大韓韓醫學方劑學會誌 Vol.15 No.2
The purpose of this study was to investigate the anti-oxidant effect of Cynomorium songaricum. The extract of Cynomorii Herba was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity, HepG2 cell viability and $H_2O_2$-induced cytotoxicity by a modified MTT assay. DPPH radical scavenging activity was measured after 30 minutes. The extract was tested by 1. 5, 10, 50, 100 and 500 ${\mu}g/ml$ concentrations. HepG2 cell viability by a modified MTT assay was measured in the concentrations of 10, 50, 100, 250, 500 ug/ml for 24 h. The results showed that the extract scavenged DPPH radical up to 52.2% with 50 ug/ml concentration. The extract did not reduced the cell viability and $H_2O_2-induced$ cytotoxicity (69.4%) was blocked by the extract in the concentrations of 50, 100, 250 and 500 ${\mu}g/ml$. In conclusion, the extract of Cynomorii Herba has potent antioxidant activity.
정영윤,최유연,양웅모,안광석 대한융합한의학회 2023 대한융합한의학회지 Vol.5 No.1
Objectives : Alcohol-induced liver disease advances as to reactive oxygen species (ROS) and cellular lipid peroxidation increase. We examined the hepatoprotective effects of Zingiber officinale Roscoe rhizome extract (ZR), Pueraria lobata Ohwi flower extracts (PF), and a newly developed herbal juice (HJ), which was a combination of ZR and PF extracts, against ethanol-induced hepatotoxicity. Methods: The study utilized the human hepatoma cell line HepG2 cells to validate the hepatoprotective effect of HJ (50~200 μg/mL) against ethanol (EtOH, 700 mM)-induced liver damage. Results: HJ effectively reduced the protein expression of sterol regulatory element-binding transcription factor 1, adiponectin, and AMP-activated protein kinase in EtOH-induced HepG2 cells. The levels of ROS, total cholesterol, and triglycerides, which are the result of various synthesis and lipogenesis processes induced by EtOH in the liver, were reduced by HJ. Furthermore, the activities of alcohol dehydrogenase and aldehyde dehydrogenase, enzymes linked to alcohol degradation, were more effectively downregulated by HJ treatment compared to treatment with ZR and PF alone, all without causing cytotoxic effects. Conclusions: HJ protects the liver by inhibiting EtOH-induced lipogenesis, lowering ROS generation, and improving alcohol degradation, which is more effective than ZR and PF alone. Further, in vivo experiments can offer additional evidence regarding the effectiveness, safety, and underlying mechanism of action of HJ.
김미혜,최태양,양웅모,Mi Hye Kim,Tae Yang Choi,Woong Mo Yang 대한융합한의학회 2021 대한융합한의학회지 Vol.1 No.1
Objectives: Excessive accumulation of fat on specific region, regarded as localized fat, is one of the serious problems and well-known risk factors of health. Recently, an interest in health and aesthetics is growing by treating lipolytic injection. Polygonatum sibiricum Rehd (PS) has been known to have anti-oxidant, -aging and -atherosclerotic effects. In this study, we investigated the lipolytic effects of PS pharmacopuncture in obese mice. Methods: Male C57BL/6J mice was fed with high fat diet to induce obesity for 12 weeks. PS pharmacopuncture was dissolved in saline by adjusting pH 7. 100 μL of PS pharmacopunture was injected subcutaneously into the left side inguinal fat pad, while saline was injected into the right side inguinal fat pad in mice as self-control. Samples were treated 3 times per weeks for 2 weeks. Results: PS pharmacopunture significantly decreased the inguinal fat weight compared to left side inguinal fat pad. Decrease rate of PS pharmacopuncture was about 21%. In addition, the diameter of adipocyte in inguinal fat tissues was significantly reduced by 17% compared to saline-injected side. There was no sign of toxicity through whole experiments. Conclusion: The present study indiates that PS pharmacopunture could be a material derived from natural herb as a lipolytic injection for decreasing localized fat.
호흡기를 통한 약액 전달을 위한 진세노사이드 초미세입자 분무장치 제작
이병철,박진수,양웅모,Byung Chul Lee,Jin Soo Park,Woong Mo Yang 대한융합한의학회 2021 대한융합한의학회지 Vol.2 No.1
Objectives: The purpose of this study is to fabricate an ultra-fine ginsenoside particle atomizer that can provide a new treatment method by delivering ginsenoside components that have a therapeutic effect on respiratory diseases directly to the lungs. Methods: We fabricated the AAO vibrating mesh by using the micromachining process. The starting substrate of an AAO wafer has a 350nm pore diameter with 50㎛ thickness. A photomask having several 5㎛ opening holes with a 100㎛ pitch was used to separate each nanopore nozzle. The photoresist structure was optimized to pattern the nozzle area during the lift-off process precisely. The commercial vibrating mesh was removed from OMRON's NE-U100 product, and the fabricated AAO vibrating mesh was installed. A diluted sample of 20mL with 30% red ginseng concentrate was prepared to atomize from the device. Results: As a result of liquid chromatography analysis before spraying the ginsenoside solution, ginsenoside components such as 20S-Rg3, 20R-Rg3, and Rg5 were detected. After spraying through the AAO vibrating mesh, ginsenosides of the same component could be detected. Conclusion: A nutrient solution containing ginsenosides was successfully sprayed through the AAO vibrating mesh with 350 nm selective pores. In particular, during the atomizing experiment of ginsenoside drug solution having excellent efficacy in respiratory diseases, it was confirmed that atomizing through the AAO vibrating mesh while maintaining most of the active ingredients was carried out.