호흡기를 통한 약액 전달을 위한 진세노사이드 초미세입자 분무장치 제작

이병철,박진수,양웅모,Byung Chul Lee,Jin Soo Park,Woong Mo Yang 대한융합한의학회 2021 대한융합한의학회지 Vol.2 No.1

Objectives: The purpose of this study is to fabricate an ultra-fine ginsenoside particle atomizer that can provide a new treatment method by delivering ginsenoside components that have a therapeutic effect on respiratory diseases directly to the lungs. Methods: We fabricated the AAO vibrating mesh by using the micromachining process. The starting substrate of an AAO wafer has a 350nm pore diameter with 50㎛ thickness. A photomask having several 5㎛ opening holes with a 100㎛ pitch was used to separate each nanopore nozzle. The photoresist structure was optimized to pattern the nozzle area during the lift-off process precisely. The commercial vibrating mesh was removed from OMRON's NE-U100 product, and the fabricated AAO vibrating mesh was installed. A diluted sample of 20mL with 30% red ginseng concentrate was prepared to atomize from the device. Results: As a result of liquid chromatography analysis before spraying the ginsenoside solution, ginsenoside components such as 20S-Rg3, 20R-Rg3, and Rg5 were detected. After spraying through the AAO vibrating mesh, ginsenosides of the same component could be detected. Conclusion: A nutrient solution containing ginsenosides was successfully sprayed through the AAO vibrating mesh with 350 nm selective pores. In particular, during the atomizing experiment of ginsenoside drug solution having excellent efficacy in respiratory diseases, it was confirmed that atomizing through the AAO vibrating mesh while maintaining most of the active ingredients was carried out.

측만증에 대한 도인 운동요법의 효과 - 체계적 문헌 고찰 -

김은정,성원석,박서현,Eun-Jung Kim,Won-Suk Sung,Seo-Hyun Park 대한융합한의학회 2021 대한융합한의학회지 Vol.2 No.1

Objectives: This study aimed to review the effects of Daoyin exercise on scoliosis in clinical studies. Methods: In this study, we reviewed 9 electronic databases (Pubmed, CAJ, EMBASE, Cochrane Library, CiNii, KISS, KMBASE, OASIS, ScienceON) on May 22, 2021. We included only randomized controlled trials (RCTs) which evaluated the effect of Daoyin exercise on scolisos. The methodological quality of the included studies was checked using the Cochrane risk of bias tool. Results: After screening, eight RCTs fulfilled the inclusion criteria. The results of the meta-analysis showed that Daoyin exercise improves the measurement value of Cobb's angle, SRS-22, pulmoary function, physiological curvature, muscle strength, effective rate in scoliosis patients. Conclusion: We concluded that Daoyin exercise has therapeutic effects in relief of imbalance, improvement of symptoms, improvement of function, muscle strength for scoliosis. However, the quality of the original articles was low and the number of papers included were few. Thus, to confirm these results, further well-designed RCTs should be conducted.

아토피 피부염에서 한약 외용제와 양약 병용 치료의 효과: 체계적 문헌고찰 및 메타분석

이지은,박수진,전서연,김규석,Ji Eun Lee,Su Jin Park,Seo Yeon Jun,Kyuseok Kim 대한융합한의학회 2023 대한융합한의학회지 Vol.5 No.1

Objectives: This study was conducted to evaluate the effect of combination treatment of herbal medicine for external use and western medicine for atopic dermatitis (AD). Methods: We searched randomized controlled trials (RCTs) which assess the effect of combination treatment of herbal and western medicine for AD through 8 electronic databases from the start to December 2022. The data synthesis was conducted by using Review Manager (RevMan, ver.5.4.1) and Cochrane risk-of-bias tool was used to evaluate the risk of bias. Results: 13 RCTs were included. The combination treatment group had significantly higher total efficacy rate(p<0.0001) and lower SCORAD score (p<0.00001) than the western medicine treatment group. The adverse event rate was also significantly lower in the combination treatment than the western medicine treatment group (p<0.0001). But there was no significant difference in recurrence rate (p=0.09). Conclusion: This study demonstrates that the combination treatment of herbal and western medicine could be safe and effective for AD. However, due to limits of included studies such as high heterogeneity between the literature and unclear risk of bias, further studies are warranted.

긴병꽃풀 추출물의 oxazolone-유도 아토피 피부염 개선 효과

김대용 대한융합한의학회 2023 대한융합한의학회지 Vol.5 No.2

Objectives: This study aimed to investigate the anti-atopic properties of Glechoma hederacea var. longituba extracts (GHE) in murine atopic dermatitis model. Methods: BALB/c mouse ear stimulated with oxazolone (OX) for 4 weeks, then 1% GHE was topically applied every two days for 3 weeks to mouse. Ear thickness was measured by a digital thickness gauge. The ears tissues were collected and subjected to hematoxylin-eosin (H&E) and toluidine blue (TB) staining. Results: Treatment with GHE successfully alleviated the symptoms of atopic dermatitis, such as erythema, horny substance, and swelling. The infiltration of lymphocytes and mast cells were significantly reduced following GHE treatment. Conclusion: Taken together, our results showed that GHE possessed the potential to be a novel immunomodulatory drug against atopic dermatitis.

가자(訶子)의 급성 췌장염 보호 효과

권빛나,김동욱,배기상 대한융합한의학회 2023 대한융합한의학회지 Vol.5 No.2

Objectives: Terminalia chebula Retzins (TC) has been used as a traditional remedy to treat gastrointestinal infectious and inflammatory diseases. However, the protective effect of TC on acute pancreatitis (AP) has not been studied. In this study, we tried to investigate the protective effect of TC water extract on cerulein-induced AP. Methods: To measure the protective effect of TC on AP, mice were injected with cerulein (50 μg/kg) hourly for 6 times. TC water extract (200 or 400 mg/kg) or saline was administered orally 1 h before the first injection of cerulein. The mice were sacrificed at 6 h after the last injection of cerulein. Pancreas tissues were taken for further analysis. Results: The administration of TC water extract showed an inhibitory effect on the increase of pancreas weight/body weight ratio and mitigated pancreatic damage in mice. Also, mRNA level of pro-inflammatory cytokines was inhibited by administration of TC water extract. Conclusion: Taken together, we found that administration of TC water extract ameliorates the severity of cerulein-induced AP, which suggests the potential to be an effective treatment on AP.

난소 절제 및 고지방식이 투여로 유도된 갱년기 비만 쥐에서의 음양곽(淫羊藿)추출물의 효능 평가

김미혜 대한융합한의학회 2023 대한융합한의학회지 Vol.5 No.2

Objectives: The purpose of this study was to investigate the ameliorative effects of the Epimedium koreanum Nakai (EK) on menopausal obesity in mice. Methods: To induce the menopausal obesity, female C57BL/6J mice were ovariectomized and fed with high fat diet for 12 weeks. 17β-estradiol was injected as a positive control and the extract of EK was orally administered 5 times per week for 6 weeks. Body weight, uterine weight and visceral fat weight were measured. The size of the adipocyte in visceral fat tissues was estimated by Hematoxylin and eosin staining. Fasting glucose level was estimated in serum. Results: Body weight and visceral fat weight were significantly decreased by EK treatment, while the uterine weight/body weight was increased in high fat diet-fed ovariectomized mice. The diameter of adipocyte in the visceral fat tissues was markedly reduced in EK-treated menopausal obese mice. In addition, the fasting blood glucose level was inhibited by oral EK administration. Conclusion: In conclusion, these results showed that EK has ameliorative effects on overweight after menopause. EK could be one of the alternative therapy for treating menopausal obesity.

소아청소년의 게임장애와 중독

이주엽 대한융합한의학회 2023 대한융합한의학회지 Vol.5 No.1

Objectives: Gaming disorder has been viewed as a disease in the DSM-5 and ICD-11. Its essential symptoms are loss of control over gaming, gaming becoming a markedly prioritized activity over other activities of daily living, and continued and excessive use of gaming despite negative problems occurring. Methods: Children and adolescents are especially vulnerable to gaming disorder because the striatal pathways related to reward develop earlier than the control regions of the prefrontal cortex. It is also associated with decreased dopamine D2 receptors. Addiction is related to ‘want’ and is explained by incentive-sensitization. In addition, allostasis, in which homeostasis is continuously achieved at a new target value, is also related to gaming disorder. In addition, personality causes, unchangeable factors, and external factors can influence on the onset of gaming disorder. Results: Prevention is the best solution for gaming disorder, and the role of parents is important. For gaming disorder, bupropion is used, cognitive-behavioral therapy and family-based therapy are also beneficial. Herbal medicine treatment such as Antler velvet and ginseng can be effective. Electroacupuncture and acupuncture using PC6, SP6, and LR3 has a correlation with relieving Internet craving. Ear-acupuncture was also effective in treating addiction. Conclusion: Psychologically, 'want' is an intense longing for reward and motivation, and is related to addiction. This ‘want’ may rather be related to avoidance, and game addiction in children and adolescents may be due to wanting to escape from academic stress or avoidance of comparison. Therefore, the importance of ‘like’, which gives pleasure in itself, increases. It can also be explained with Sasang Constitutional Medicine.

Protective effects against alcoholic liver damage: potential of herbal juice (HJ), blend of Zingiber officinale Roscoe and Pueraria lobata Ohwi extracts

정영윤,최유연,양웅모,안광석 대한융합한의학회 2023 대한융합한의학회지 Vol.5 No.1

Objectives : Alcohol-induced liver disease advances as to reactive oxygen species (ROS) and cellular lipid peroxidation increase. We examined the hepatoprotective effects of Zingiber officinale Roscoe rhizome extract (ZR), Pueraria lobata Ohwi flower extracts (PF), and a newly developed herbal juice (HJ), which was a combination of ZR and PF extracts, against ethanol-induced hepatotoxicity. Methods: The study utilized the human hepatoma cell line HepG2 cells to validate the hepatoprotective effect of HJ (50~200 μg/mL) against ethanol (EtOH, 700 mM)-induced liver damage. Results: HJ effectively reduced the protein expression of sterol regulatory element-binding transcription factor 1, adiponectin, and AMP-activated protein kinase in EtOH-induced HepG2 cells. The levels of ROS, total cholesterol, and triglycerides, which are the result of various synthesis and lipogenesis processes induced by EtOH in the liver, were reduced by HJ. Furthermore, the activities of alcohol dehydrogenase and aldehyde dehydrogenase, enzymes linked to alcohol degradation, were more effectively downregulated by HJ treatment compared to treatment with ZR and PF alone, all without causing cytotoxic effects. Conclusions: HJ protects the liver by inhibiting EtOH-induced lipogenesis, lowering ROS generation, and improving alcohol degradation, which is more effective than ZR and PF alone. Further, in vivo experiments can offer additional evidence regarding the effectiveness, safety, and underlying mechanism of action of HJ.

천연물 외용제를 적용한 아토피피부염 증례 및 임상 연구 고찰

서광일,김규석,Gwang-Yeel Seo,Kyuseok Kim 대한융합한의학회 2021 대한융합한의학회지 Vol.2 No.1

Objectives: To investigate the characteristics of topical agents including natural products using for atopic dermatitis treatment via a review on the cases and clinical trials. Methods: Through a search on Pubmed, EMBASE, Cochrane library, DBPIA, NDSL, and OASIS, we selected papers that applied natural products externally for atopic dermatitis and analyzed them. Results: Finally, Seventeen papers were selected. As the primary outcome, changes in the SCORAD and EASI, were the most used. The most frequent type of external formulation was cream. In terms of the frequency of use, it was used at least twice a day in all studies. When evaluated based on SCORAD, EASI, SH and TEWL, the improvement effect on atopic dermatitis was highest when the ointment formulation and the moisturizer formulation were used. A few side effects of topical agents including natural products were observed, but most were mild symptoms and no serious side effects occurred. Conclusion: This study is meaningful in that it has drawn a significant trend for studies that have been published in the last 5 years that contain a single intervention for topical agents including natural products, and that it presents the direction and evidence for the application of external therapy to increase the therapeutic effect of atopic dermatitis.

고지방식이를 급여한 마우스의 간과 HepG2 세포에서 TJGB의 효과에 대한 연구

김희영,박예진,안효진 대한융합한의학회 2023 대한융합한의학회지 Vol.5 No.1

Objectives: This study was performed to investigate the effect of TJGB on the liver of high-fat diet (HFD)-fed mice and the cell viability of HepG2 cells. Methods: After a week adaptation, 8-week-old C57BL/6N mice were fed with a 45% HFD or normal diet for 3 weeks. For the next 9 weeks, the mice were divided into 6 groups: normal diet group; HFD group; HFD plus orlistat group; HFD plus Ephedra sinica Stapf (ES) group; HFD plus low dose of TJGB group; HFD plus high dose of TJGB group. To estimate the effect of TJGB in the liver of HFD-fed mice, the protein expressions of phospho-acetyl-CoA carboxylase (p-ACC) and liver X Receptor (LXR) were determined by Western blot assay. The cell viability of ES and TJG was also evaluated in HepG2 cells. Results: The administration of TJGB had little effect on the protein expressions of p-ACC and LXR in the liver of HFD-fed mice. And the cytotoxicity was showed above 7.8 μg/mL in HepG2 cells. Conclusion: Further research is needed to evaluate the mechanism of TJGB on hepatic steatosis and cytotoxicity in HepG2 cells.