IgE/BSA가 자극한 골수유래 비만 세포에 대한 감태 Ethyl Acetate 분획물의 항알러지 효능

안긴내,김현수,신은지,김민주,한희진,전유진,지영흔,한의정 한국키틴키토산학회 2018 한국키틴키토산학회지 Vol.23 No.4

In this study, we investigated the anti-allergic effect of the ethyl acetate fraction of Ecklonia cava (EC-EtoAc) on the immunoglobulin E (IgE)/bovine serum albumin (BSA)-mediated activation of bone marrow-derived cultured mast cells (BMCMCs). We revealed that the 62.5 μg/ml of EC-fractions (EC-CHCl3, EC-Hexane and EC-EtoAc) inhibited IgE/BSAactivated β-hexosaminidase release from BMCMCs without cytotoxicity. Especially, EC-EtoAc showed the higher β- hexosaminidase release than the others. Also, EC-EtoAc reduced the expression levels of cytokines such as interleukin (IL)-1β, IL-4, IL-5, IL-6, IL-10, IL-13, interferon (IFN)-γ and tumor necrosis factor (TNF)-α and a chemokine, thymus- and activationregulated chemokine (TARC), compared to the only IgE/BSA-treated BMCMCs. Furthermore, EC-EtoAc significantly prevented the binding of IgE to Fc epsilon receptor (FcεR)I and reduced the FcεRI expression on the sensitized BMCMCs. Taken together, these results suggest that E. cava may be the natural agent with beneficial potentials for the treatment of type I allergic diseases induced by mast cell activation.

Comet Assay 를 이용한 제주 자생 녹조류 추출물의 세포내 DNA 손상 억제 활성 효과

안긴내,이승홍,차선희,고석천,전유진 제주대학교 방사능이용연구소 2006 방사선응용과학연구소논문집 Vol.20 No.-

UVB protective effect of hydrolysate derived Hermetia illucens in human keratinocytes

안긴내,박서영,Yoo Si-won,이서희,Choi Jong Min,Song Jin Woo,Han Eui Jeong 한국키틴키토산학회 2022 한국키틴키토산학회지 Vol.27 No.2

In this study, we prepared enzymatic extracts using trypsin and pepsin from Hermetia illucens (THI and PHI) and evaluated their effects on oxidative stress in ultraviolet B (UVB)-stimulated keratinocytes. PHI showed a higher extraction yield than THI, but the protein content of THI was higher. When the antioxidant activity was assessed in vitro, both indicated an effect of 25% or more compared to the positive control (L-ascorbic acid). In addition, both THI and PHI decreased the generation of intracellular reactive oxygen species (ROS) in UVB-stimulated keratinocytes, without cytotoxicity at all concentrations used. We explored the efficacy of THI. Results demonstrated that THI effectively reduced apoptosis and sub-G1 DNA content. In addition, it was found that UVB-stimulated keratinocytes protect skin keratinocytes from UVB by regulating the apoptotic pathway, MAPKs, and NF-kB signaling pathways. In conclusion, these results show that trypsin hydrolysate of H. illucens protects cells from oxidative stress in UVB-irradiated human keratinocytes.

넙치로부터 분리된 유산균에 미치는 키토산 분자량에 따른 효과와 항균효과

안긴내 ( Gin Nae Ahn ),이원우 ( Won Woo Lee ),안창범 ( Chang Bum Ahn ),전유진 ( You Jin Jeon ) 한국키틴키토산학회 2014 한국키틴키토산학회지 Vol.19 No.4

Chitosan and its oligosaccharides have shown antimicrobial effects against pathogenic bacteria inducing fish diseases. Lactic acid bacterium (LABs) known as probiotics play important roles in the regulation or maintenance of normal flora and pathogenic bacteria. In this study, we investigated the potential of chitosan oligosaccharides (COSs) as natural prebiotics by evaluating their capacities on the growth of both lactic acid bacterium (LAB) and Vibrio anguillarum (V. anguillarum, Listonella anguillarum). First of all, we prepared three COSs (COS1; molecular weight (MW) 1 KDa>, COS2; MW 1~5 KDa, COS3; MW 5~10 KDa) by enzymatic hydrolysis of chitosan and isolated lactobacillus brevis (L. brevis) BK4 from flounder fish intestine. Among the chitosan and its three MW fractions, the two lower MW fractions, COS1 and COS2 markedly decreased pH by increasing the production of organic acids, especially lactic acid in the BK4-cultured mediums. In contrast, the higher MW fraction, COS3 showed no significant difference in pH. Also, COS1 and COS2 significantly increased the growth of BK4 at all the concentrations according to the inoculation times, whereas COS3 and chitosan inhibited them. Furthermore, COS2 showed the strong antibacterial activities as markedly enhancing the inhibitory effect against the growth of V. anguillarum in co-cultured system of BK4 and V. anguillarum compared to that of pure-cultured system. In conclusion, the relatively lower COSs led to the growth of BK4 with the production of lactic acid and improved the anti-bacterial activity against V. anguillarum. This study suggests that the lower MW fractions of COSs can be used as a useful prebiotic candidate to improve fish diseases as the animal feed additives.

전통발효식품으로부터 분리된 유산균의 생육에 미치는 키토산의 분자량에 따른 영향

안긴내 ( Gin Nae Ahn ),이원우 ( Wonwoo Lee ),전유진 ( You Jin Jeon ) 한국키틴키토산학회 2014 한국키틴키토산학회지 Vol.19 No.3

한국 전통 식품인 김치와 젓갈, 탁주로부터 6종의 유산균을 분리하여 동정하였고, 분리된 유산균의 생육에 대한 chitosan 및 분자량별로 분리된 그것의 COSs의 효능을 검증하기 위해 유산균 배양액의 pH 변화 및 유기산 분석과 유산균의 성장을 확인하였다. 본 연구결과에서는 chitosan과 고분자량의 COS3가 분리된 대부분의 유산균에 대한 현저한 성장 저해 효과를 보였으나, 분자량이 낮은 COS1과 COS2는 모든 유산균의 성장을 저해시키지 않았고, 현저하게 촉진시킴으로서 유기산의 생성을 유도하여 pH를 저하시켰다는 것을 확인했다. 이 모든 결과를 미루어볼 때, 본 연구는 유산균의 성장을 저해하는 chitosan을 저분자화 시켜 제조한 COS1과 COS2가 장내 유용 세균으로 알려진 유산균의 성장을 촉진시켜 어류 양식 산업에 있어 유산균제제와 함께 첨가 시 장내 면역을 증강시키는 유용한 사료첨가제로서 사용될 수 있을 것으로 사료되며, 더 나아가 인간 장내의 대량으로 존재하는 유용균에 대한 prebiotics로서 작용될 수 있음을 제시한다. Chitosan and its oligosaccharides have shown antimicrobial effects against pathogenic bacteria inducing fish diseases. Lactic acid bacterium (LABs) known as probiotics play important roles in the regulation or maintenance of normal flora and pathogenic bacteria. In this study, we elucidated the potential of chitosan and its oligosaccharides as natural prebiotics inducing the growth of lactic acid bacterium (LABs). First of all, we prepared three chitosan oligosaccharides (COSs, COS1; molecular weight (MW)1 KDa>, COS2; MW 1~5 KDa, COS3; MW 5~10 KDa) by enzymatic hydrolysis of chitosan and isolated the 6 kinds of proteolytic LABs (BK1, BK2, BK14, BK18, BK19, and BK20) from Korean traditional foods such as Kimchi, fish fermented foods and rice wine. Among the chitosan and its three MW fractions, the two low MW fractions, COS1 and COS2 markedly decreased pH by increasing the production of organic acids, especially lactic acid and formic acid in the all LABs-cultured mediums. In contrast, the high MW fraction, COS3 significantly decreased pH with the increment of the lactic acid produced in the only BK19-cultured medium and did not affect to the others. Also, COS1 and COS2 significantly induced the growth ofLABs at all the concentrations, whereas COS3 and chitosan inhibited them. In particular, COS1 showed the highest growth effects for the 6 kinds of LABs from fish fermented foods (Jeotgal), compared to those of two other fractions and chitosan. Itwas better than them of LABs isolated from Kimchi (BK1 and BK2). Taken together, the low MW fractions of chitosan led to the growth of six LABs with the change of pH and the production of organic acids. This study suggests that the low MW fractions of COSs can be used as useful prebiotics to improve fish diseases as the animal feeds.

Sargassum horneri ethanol extract improves the type-1 allergic responses in IgE-activated mast cells and house dust mite (HDM)/2,4-dinitrochlorobenzene (DNCB)-stimulated NC/Nga mice model

Ginnae Ahn(안긴내),Eui Jeong Han,Min Ju Kim,Disanayaka Mudiyanselage Dines,Mawalle Kankanamge Hasitha Mad,Ilekuttige Priyan Shanura Fern,Hyun-Soo Kim,Seo-Young Kim,You-Jin Jeon,Youngheun Jee 한국수산과학회 양식분과 2020 한국수산과학회 양식분과 학술대회 Vol.2020 No.7

Hydrogen peroxide로 유도된 산화적 손상에 대한 물레고둥 효소가수분해물의 간 보호 효능 평가

한의정,안긴내 한국키틴키토산학회 2022 한국키틴키토산학회지 Vol.27 No.1

The purpose of this study was to investigate the cytoprotective effects of a neutrase enzymatic hydrolysate derived from Buccinum striatissumum (BSMN) against hydrogen peroxide (H2O2)-induced oxidative damage in hepatocytes. BSMN effectively scavenged the 2,2-azino-bis (3-ethylbenzthiazoline)-6-sulfonic acid radical (ABTS) and increased the oxygen radical absorbance capacity (ORAC) value by incresing the reducing power. Also, BSMN markedly increased the cell viabiltiy by inhibiting the intracellualr reactive oxygen species (ROS) production in H2O2-stimulated hepatocytes. In addition, BSMN decreased the sub-G1 DNA contents and apoptotic body formation increased by regulating the apoptosis related proteins (Bcl-2, Bax and p53). Moreover, BSMN activated the Nrf2/HO-1 signaling pathway by suppressing the activation of NF-κB and ERK/p38 signaling in H2O2-stimulated hepatocytes. These results suggest BSMN protects hepatocytes against oxidative stress caused by H2O2 stimulation

해조류를 이용한 효소적 추출물의 기능성

전유진,안긴내 동아시아식생활학회 2007 동아시아식생활학회 학술발표대회논문집 Vol.- No.-

피부각질형성세포에서 TSLP 조절에 의한 감태의 항염증 효과

강나래,안긴내,한의정,신은지,한희진,전유진,안창범 한국키틴키토산학회 2017 한국키틴키토산학회지 Vol.22 No.4

Human thymic stromal lymphopoietin (TSLP), secreted from keratinocytes, activates the inflammatory cytokines and directly stimulates the sensory neurons triggering scratching behavior, and finally leads to the development of skin inflammatory diseases such as atopic dermatitis. In this study, we verified the anti-inflammatory effects of Ecklonia cava ethanol extracts (ECE) through TSLP down-regulation in tumor necrosis factor (TNF)-α/interferon (IFN)-γ-induced inflammation in HaCaT, a human keratinocytes. Also, we observed the change of scratching behavior of mice with atopic dermatitis after the oral administration of ECE. First, ECE reduced the mRNA expression and secretion levels of TSLP in TNF-α/IFN-γ-stimulated HaCaT keratinocytes. Also, ECE inhibited the mRNA expression or secretion levels of inflammatory cytokines such as Interleukin (IL)-4, -5, -13, and TNF- α as well as the mRNA expression level of thymus and activation regulated chemokine (TARC), a chemokine. Moreover, ECE significantly reduced the scratching frequency in NC/Nga mice with spontaneous atopy diseases. According to these results, ECE has the anti-inflammatory effect and a probability as a natural materials for the prevention of skin diseases.

Ultraviolet B를 조사한 HaCaT keratinocytes에서 다시마 부산물 열수 추출물의 염증 억제 효과

유시원,안긴내 한국키틴키토산학회 2023 한국키틴키토산학회지 Vol.28 No.3

The results of this study confirmed the inhibitory effect of Saccharina japonica hot water extract (SJH) on the inflammatory response when HaCaT keratinocytes were irradiated with UV-B. SJH significantly improved cell viability without cytotoxicity and also reduced the NO content increased by UV-B irradiation. In addition, SJH effectively reduced the mRNA expression of cytokines and chemokines increased by UV-B irradiation, and also reduced the protein expression levels of related factors iNOS and COX-2 through inhibition of Nuclear Factor Kappa B (NF-κB) signaling pathway activation. Additionally, it effectively inhibited the expression of proteins (NLR family pyrin domain containing 3 (NLRP3), Apoptosis-associated speck-like protein containing a C-terminal caspase recruitment domain (ASC), interleukin 1-beta-converting enzyme (Caspase-1), Interleukin(IL)-18, and IL-1β)) related to the NLRP3 signaling pathway, an inflammation-related protein. These research results suggest that SJH can effectively suppress the inflammatory response that can occur due to UV-B irradiation.