꿀벌과 서양뒤영벌에 대한 농약의 독성평가

안기수(Ki-Su Ahn),오만균(Mann-Gyun Oh),안희근(Hee-Geun Ahn),윤창만(Changmann Yoon),김길하(Gil-Hah Kim) 한국농약과학회 2008 농약과학회지 Vol.12 No.4

This study was performed to evaluate the acute toxicity and residual toxicity of the 69 kinds of agrochemicals (41 insecticides, 18 fungicides, and 10 acaricides) against honeybee, Apis mellifera and bumblebee, Bombus terrestris. According to the IOBC standard, the toxicity showed below 30 % was classified as non-toxic. Among 41 insecticides, five insecticides (acetamiprid, chlorfenapyr, thiacloprid, milbemectin, and buprofezin+amitraz) against the honeybee; eight insecticides (methomyl, thiodicarb, acetamiprid, chlorfenapyr, thiacloprid, abamectin, spinosad, buprofezin+amitraz) against the bumblebee did not show any toxic effect. Therefore, it thought to being safe. Other 18 fungicides and 10 acaricides were safe against the honeybee and bumblebee. In residual toxicity against the honeybee, eight insecticides (dichlorvos, methomyl, imidachlorprid, emamectin benzoate, spinosad, cartap hydrochloride, chlorfenapyr, and endosulfan) among 41 insecticides tested were safe at three days after treatment; however, sixteen insecticides (dimethoate, fenitrothion, fenthion, methidathion, phenthoate, pyraclofos, fenpropathrin, clothianidin, dinotefuran, thiamethoxam, abamectin, acetamiprid+ethofenprox, acetamiprid+indoxacarb, bifenthrin+imidacloplid, ethofenprox+phenthoate, imidacloprid+methiocarb) stil1 remain high toxicity at eleven days after treatment. Against the bumblebee, residual toxicity showed as safe in seven insecticides (dimethoate, methidation, a-cypermethion, ethofenprox, indoxcarb, chlorpyrifos+a-cypermethrin, esfenvalerate+fenitrochion) at three days after treatment; however, eight insecticides (fenitrothion, pyraclofos, clothianidin, fipronil, acetamiprid+ethofenprox, chlorpyrifos+bifenthrin, ethofenprox+phenthoate, imidacloprid+methiocarb) still showed high toxicity at seven days after treatment. From above results, it will be useful information to select insecticides being safe and effectivc against the honeybee and bumblebee.

난총채벌레의 살충제 감수성

안기수(Ki-Su Ahn),이기열(Ki-Yeol Lee),강효중(Hyu-Jung Kang),박성규(Sung-Kyu Park),김길하(Gil-Hah Kim) 한국농약과학회 2002 농약과학회지 Vol.6 No.4

This study was carried out to investigate the toxicities of 22 insecticides to Dichromothrips smithi. Insecticidal activity was evaluated by testing systemic action and residual effect in laboratory. All insecticides used in this study did not affect on the egg of D. smithi, although organophosphates such as fenitrothion, fenthion, methidathion, phenthoate, and phenthoate+ethofenprox suppressed the egg hatchability completely. On D. smithi larva fenitrothion, fenthion, methidathion, phenthoate, ethofenprox, thiamethoxam, abamectin, chlorfenapyr, cmamectin benzoate, fipronil, spinosad, and phenthoate+ethofenprox showed 100% insecticidal activity. On D. smithi adult fenitrothion, fenthion, methidathion, phenthoate, ethofenprox, abamectin, emamectin benzoate, fipronil, spinosad, and phenthoate+ethofenprox showed 100% insecticidal activity. Root-uptake systemic effects of phenthoate on the larva of D. smithi was 43.3%. Whereas, systemic effect of other insecticides was less than 20%. Insecticide with more than 80% residual effect for 7 days after treatment were fenitrothion, fenthion, methidathion, phenthoate, ethofenprox, emamectin benzoate, fipronil, spinosad, and phenthoate.

딸기에 등록된 살충제의 꿀벌에 대한 급성 및 엽상잔류독성

안기수(Ki-Su Ahn),윤창만(Changmann Yoon),김기현(Ki-Hyun Kim),남상영(Sang-Young Nam),오만균(Man-Gyun Oh),김길하(Gil-Hah Kim) 한국농약과학회 2013 농약과학회지 Vol.17 No.3

This study was performed to evaluate the spray toxicity and leaf residual toxicity of 52 kinds of insecticides registered for strawberry against adult honeybee Apis mellifera. According to the IOBC standard, the acute toxicity by spraying showed below 30% was classified as non-toxic. Among tested insecticides, 32 insecticides (flonicamid, lufenuron, novaluron, three kinds of acetamiprid, thiacloprid, milbemectin, acequinocyl, TBI-1, two kinds of chlorfenapyr, chlorfluazuron, cyenopyrafen, cyfumetofen, etoxazole, fenpyroximate, flubendiamide, flufenoxuron, hexythiazox, metaflumizone, two kinds of methoxyfenozide, DBB-2032, pyridalyl, spiromesifen, tebufenpyrad, teflubenzuron, acetamiprid + methoxyfenozide, acrinathrin + spiromesifen, bifenazate + spiromesifen, cyenopyrafen + flufenoxuron) did not show any toxic effect, it is thought to be safe. And the others (20 insecticides) showed higher toxicity to honeybee. Insecticides which showed acute toxicity higher than 90% was selected and tested the residual toxicity. All insecticides except emamectin benzoate EC, and indoxacarb SC showed 100% mortality at one day after treatment (DAT). However, the toxicities of emamectin benzoate, indoxacarb SC, and abamectin did not show until 3, 7, 14 DAT, respectively. Nine insecticides such as indoxacarb WP, thiamethoxam WG, abamectin + chlorantraniliprole SC, acetamiprid + etofenprox WP, acetamiprid + indoxacarb WP, bifenthrin + clothianidin SC, bifenthrin + imidacloprid WP, bifenazate + pyridaben SC, chlorfenapyr + clothianidin SC showed over 90% residual toxicity until 31 Day. In pouring treatment, thiamethoxam WG showed 76.9% mortality at 28 DAT and 50.0% mortality at 31 DAT. After 35 days, thiamethoxam WG showed no effect to honeybee. Bifenthrin + clothianidin SC and tefluthrin + thiamethoxam GR showed 57.1 and 80.0% mortality at 24 DAT, respectively. In spraying treatment, thiamethoxam WG and bifenthrin+clothianidin SC showed very high residual toxicity with 100% mortality in thirty-five DAT. After spraying treatment with thiamethoxam WG, bifenthrin+clothianidin SC, bifenthrin + imidacloprid WP, thiamethoxam WG showed 100% residual toxicity until 21 DAT and there was no activity after 28 DAT. Bifenthrin+clothianidin SC and bifenthrin+imidacloprid WP showed very high residual toxicity until 49 DAT.

국내시판 살충제에 대한 미국선녀벌레의 감수성

안기수(Ki-Su Ahn),이관석(Gwan-Seok Lee),이경희(Kyeong-Hee Lee),송명규(Myung-Kyu Song),임상철(Sang-Cheol Lim),김길하(Gil-Hah Kim) 한국농약과학회 2011 농약과학회지 Vol.15 No.3

Insecticidal activity of 31 registered insecticides was tested against Metcalfa pruinosa adults. All experiments were conducted at the recommended concentration (ppm) of each insecticide. Among them, 16 insecticides from organophosphates (dichlorvos, fenitrothion, fenthion, methidathion, phenthoate), carbamates (methomyl), pyrethroids (α-cypemethrin, deltamethrin, fenpropathrin, γ-cyhalothrin), neonicotinoids (acetamiprid, clothianidin, dinotefuran, imidacloprid, thiarnethoxam), and other (endosulfan) showed 100% mortality by spraying on the body of M. pruinosa adults. Dichlorvos, fenitrothion, fenthion, methidathion, phenthoate and endosulfan showed 100% mortality by plant-dipping method. The residual effect was showed 100% mortality in four insecticides (fenitrothion, fenthion, methidathion, phenthoate) at one day after treatment, and three insecticides (fenitrothion, methidathion, phenthoate) were showed the mortality of 90% at three days after treatment.

갈색여치에 대한 살충제의 감수성

안기수(Ki-Su Ahn),양정오(Jeong-Oh Yang),노두진(Doo-Jin Noh),윤창만(Changmann Yoon),김길하(Gil-Hah Kim) 한국농약과학회 2007 농약과학회지 Vol.11 No.3

Insecticidal activity of 33 registered insecticides was tested against last nymphal instars and adults of ussur brown katydid (Paratlanticus ussuriensis). All experiments were tested at the recommended concentration of each insecticides by producer. Acephate, chlorpyrifos, diazinon, EPN and fenitrothion which were organophates, and a mixture combined with chlorpyrifos+α-cypemethrin showed 100% mortality of P. ussuriensis. But fipronil showed only 100% mortality in leaf-dipping method. Carbamates insecticidal groups, benfuracarb and furathiocarb were showed over 80% and phenthoate was 60~80% in mortality of P. ussuriensis. Among the mixture, etofenprox+diazinon and esfenvalerate+fenitrothion were showed 60-80% against last nymphal instars of P. ussuriensis. Otherwise, acephate, chlorpyrifos, diazinon, EPN and fenitrothion were showed 100% mortality of P. ussuriensis within only 24 hours after treatment, but there was no effective after then in residual tests with leaves.

포도원에 발생하는 이슬애매미충과 이마점애매미충의 생태적 특징

안기수,김황용,이기열,황종택,김길하,Ahn, Ki-Su,Kim, Hwang-Yong,Lee, Ki-Yeol,Hwang, Jong-Tack,Kim, Gil-Hah 한국응용곤충학회 2005 한국응용곤충학회지 Vol.44 No.3

충북지역을 중심으로 이슬애매미충과 이마점애매미충의 발생생태 및 기주식물을 조사한 결과, 이들은 5월 초순부터 포도원에 들어와서 포도 잎을 가해하기 시작하며, 대체로 6월 하순과 8월 중순 경에 높은 밀도를 보였다. 이후 이슬애매미충은 10월 초순부터 월동하기 적당한 수피를 찾아서 인근 숲으로 이동하였고, 이마점애매미충은 9월 하순부터 포도원 주변의 잡초로 이동하였다. 충청북도 5개군의 발생조사에서, 두 종 모두 옥천지역에서 많이 발생하였다. 발육기간은 이마점애매미충이 이슬애매미충보다 짧았다. The occurrence and the host plants of Arboridia kakogawana and A. maculifrons in the province of Chungcheonbuk-Do were observed. They started to infest grapevine in a vineyard in early May and reached peak population two times once in late June and once in mid August in general. In preparation for overwinter, A. kakogawana moved to the nearby forest in search of a tree with bark from early October. A. maculifrons also moved to the weeds on the ridge of vineyard circumferences from the end of September. Population density of the two species were found to be the highest in Okcheon county among the five counties of Chungbuk province. Developmental period of A. kakogawana was shorter than that of A. maculifrons.

고도근시에서 스무딩을 병용한 고등각막표면연마굴절수술(Advanced Surface Ablation)의 임상성적

안기수,이승욱,이기헌,최봉준,Ki Su Ahn,MD,Seung Wuk Lee,MD,Gi Heon Lee,MD,Bong Joon Choi,MD,PhD 대한안과학회 2012 대한안과학회지 Vol.53 No.3

Purpose: To investigate the results of Advanced Surface Ablation (ASA) coupled with `smoothing` to smooth the ablation surface after covering masking fluid. Methods: ASA was performed in 61 eyes with ablation depth of more than 75 μm. The mean refractive error was -5.88 ± 1.27 D and mean ablation depth was 102.93 ± 12.06 μm. Smoothing was performed in all patients (mean depth 16.79 ± 2.43 μm, mean diameter 8.77 ± 0.16 mm). Customized Aspheric Transition zone (CATz) was used in the laser algorithm. Results: The mean refractive error was -0.29 ± 0.41 D at postoperative 6 months and 97% of patients had an UCVA of 1.0 or better at postoperative 6 months. There was no statistically significant difference in magnitude of high-order aberrations at postoperative 6 months. The magnitude of total spherical aberrations increase was 0.084 μm at postoperative 6 months (p<0.05, paired t-test). The cornea was maintained clear in the majority of eyes. Conclusions: Excellent results were obtained by ASA coupled with `smoothing` in high myopia patients with an ablation depth greater than 75 μm. J Korean Ophthalmol Soc 2012;53(3):365-371

총채벌레의 천적인 으뜸애꽃노린재에 대한 농약 독성

안기수,이기열,강효중,박성규,김길하,Ahn Ki-Su,Lee Ki-Yeol,Kang Hyu-Jung,Park Sung-Kyu,Kim Gil-Hah 한국응용곤충학회 2004 한국응용곤충학회지 Vol.43 No.3

병해충 방제약제로 등록된 51종의 농약(살충제 25종, 살비제 11종, 살균제 11종 그리고 농약보조제 4종)에 대한 으뜸애꽃노린재 약충과 성충의 독성을 조사하였다. 살충제 중에서는 flpronil, lufenuron, acetamiprid+fipronil, $\alpha$-cypermethrin+flufenoxuron, buprofezin+amitraz of 독성이 낮았고, 살비제 중에서는 acequinocyl, bifenazate, chlorfenapyr, etoxazole, fenpyroximate, flufenoxuron, milbemectin, spirodiclofen, tebufenpyrad가 독성이 낮거나 없었다. 살균제와 농약보조제는 모두 낮은 독성을 보였다. 이상의 결과로 보아 시설 병해충의 종합관리체계 에서 으뜸애꽃노린재에 독성이 적은 약제와 으뜸애꽃노린재를 함께 이용할 수 있을 것이다. Toxicities of 51 pesticides (25 insecticides, 11, acaricides, 11 fungicides and 4 adjuvants) commonly used to control greenhouse insect, mite, and disease pests were evaluated to minute pirate bug, Orius strigicollis nymphs and adults at the recommended concentration. Among 25 insecticides tested, fipronil, lufenuron, acetamiprid+fipronil, $\alpha$-cypermethrin+flufenoxuron and buprofezin + amitraz showed low toxicity to O. strigicollis. Among acaricides, acequinocyl, bifenazate, chlorfenapyr, etoxazole, fenpyroximate, flufenoxuron, milbemectin, spirodiclofen and tebufenpyrad showed low toxicity to O. strigicollis. All fungicides and adjuvants tested were very low toxicity. It may be suggested from these results that five insecticides, nine acaricides, eleven fungicides and four adjuvants could be incorporated into the integrated thrips management system with O. strigicollis in greenhouses.

꽃노랑총채벌레에 대한 Pennyroyal oil과 Spearmint oil의 훈증효과

한종빈,안기수,이총규,김길하,Han Jong-Been,Ahn Ki-Su,Lee Chong-Kyu,Kim Gil-Hah 한국응용곤충학회 2006 한국응용곤충학회지 Vol.45 No.1

꽃노랑총채벌레 성충에 대한 4종 식물정유(muguet flower, patchouli, pennyroyal, spearmint)의 훈증효과를 조사하였다. 그들 중 100%의 훈증효과를 나타낸 정유는 pennyroyal oil과 spearmint oil 이었다. 두 정유에 대한 주요 구성성분을 GC와 GC/MS로 분석한 결과, pennyroyal oil은 pulegone(100.0%), spearmint oil은 ${\beta}-myrcene(3.5%)$, limonene(12.1%), carvone(85.4%)이 주요 구성성분으로 나타났다. 이 성분의 훈증독성 시험결과 carvone과 pulegone은 $1{\mu}{\ell}/45m{\ell}$ air의 약량에서 100%의 높은 훈증독성을 나타내었다. Fumigant toxicity of four plant essential oils (muguet flower, patchouli, pennyroyal, spearmint) were tested against the adults of western flower thrips, Frankliniella occidentalis. Among them, pennyroyal and spearmint oils showed 100% mortality at $1{\mu}{\ell}/45m{\ell}$ air dose. Through the constituent analysis using GC and GC-MS, we confirmed that main constituents of pennyroyal oil were pulegone(100.0%), and spearmint oil were $\beta-myrcene(3.5%)$, limonene(12.1%), carvone(85.4%). Among them, carvone and pulegone showed 100% fumigation at $1{\mu}{\ell}/45m{\ell}$ air dose, respectively. It can concluded that two oils are potential control agents against F. occidentalis.

배추좀나방에 대한 Indoxacarb의 작용특성 및 기작

최유미,안기수,황인천,김길하,Choi Yu-Mi,Ahn Ki-Su,Hwang In-Cheon,Kim Gil-Hah 한국응용곤충학회 2004 한국응용곤충학회지 Vol.43 No.4

배추좀나방의 발육단계별 indoxacarb의 살충활성, 침투이행성 및 잔효성을 조사하였고, esterase, acetylcholinesterase, glutathione S-transferase 등의 효소활성에 미치는 영향을 검토하였다. 배추좀나방 유충에 대해서 높은 살충효과를 나타내었으나, 알과 번데기에 대해서는 살충율이 $10\%$이하이었다. 엽면침투이행성과 근부침투이행성의 효과는 없었으며, 잔효성은 10일째까지 $80\%$의 살충효과를 유지하였다. Indoxacarb는 esterase와 glutathione S-transferase의 활성은 저해하지 않았지만 acetylcholinesterase의 활성을 저해하였다. Insecticidal activity, systemic and residual effects, and effects on enzyme activities (esterase, acetylcholinesterase, glutathione S-transferase) of indoxcarb were evaluated against Plutella xylostella. The insecticide was very effective against larvae of P. xylostella. Also, indoxacarb showed only residual effect to P. xylostella when applied to vegetable leaves. It inhibited acetylcholinesterase activity, but didn't do esterase and glutathione S-transferase activities.