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고현석,정재성,손산호,이영선,고영진,송장훈 한국식물병리학회 2013 Plant Pathology Journal Vol.29 No.4
The fungus Venturia nashicola is the causal agent of scab on Asian pears. For the rapid and reliable identification as well as sensitive detection of V. nashicola, a PCR-based technique was developed. DNA fingerprints of three closely related species, V. nashicola, V. pirina,and V. inaequalis, were obtained by random amplified polymorphic DNA (RAPD) analysis. Two RAPD markers specific to V. nashicola were identified by PCR, after which two pairs of sequence characterized amplified region (SCAR) primers were designed from the nucleotide sequences of the markers. The SCAR primer pairs,designated as D12F/D12R and E11F/E11R, amplified 535-bp and 525-bp DNA fragments, respectively, only from genomic DNA of V. nashicola. The specificity of the primer sets was tested on strains representing three species of Venturia and 20 fungal plant pathogens. The nested PCR primer pair specific to V. nashicola was developed based on the sequence of the species-specific 525-bp DNA fragment amplified by primer set E11F/E11R. The internal primer pair Na11F/Na11R amplified a 235-bp fragment from V. nashicola, but not from any other fungal species tested. The nested PCR assay was sensitive enough to detect the specific fragment in 50 fg of V. nashicola DNA.
이영선,Hyun Seok Koh,손산호,고영진,정재성 한국식물병리학회 2012 Plant Pathology Journal Vol.28 No.3
Genetic diversity among Pseudomonas syringae pv. morsprunorum isolates from Prunus mume in Korea and Japan was investigated by comparative sequence analysis of the 16S rRNA gene. The strains included 24field isolates recovered from P. mume in Korea along with seven Japanese strains. Two strains isolated from P. salicina in Japan, one strain from P. avium in the United Kingdom, and the pathotype strain were also used for comparison with their 16S rRNA gene sequences. Nearly complete 16S rRNA gene sequences were sequenced in all 35 strains, and three sequence types, designated types I, II and III, were identified. Eleven strains consisting of five Korean isolates, five Japanese strains, and one strain from the United Kingdom belonged to type I, whereas the pathotype strain and another 19 Korean isolates belonged to type III. Another four Japanese strains belonged to type II. Type I showed 98.9% sequence homology with type III. Type I and II had only two heterogeneous bases. The 16S rRNA sequence types were correlated with the races of P. syringae pv. morsprunorum. Type I and II strains belonged to race 1, whereas type III isolates were included in race 2. Sequence analyses of the 16S rRNA gene from P. syringae pv. morsprunorum were useful in identifying the races and can further be used for epidemiological surveillance of this pathogen.
국내에서 개발된 참다래 신품종의 과실무름병 발생률과 병원균 검출 빈도
권신영,김경희,고영진,이영선,손산호,김성철,정재성 한국자원식물학회 2011 한국자원식물학회지 Vol.24 No.5
The incidence rates of postharvest fruit rots of four kiwifruit cultivars which were cultivated under rain-proof tunnel house at a same orchard were examined. Among them, ‘Halla-Gold’, ‘Jecy-Gold’ and ‘Jecy-Sweet’ were new cultivars bred in Korea. The disease incidence was varied with cultivars; 74.8%, 65.3%, 57.1% and 16.2% for ‘Hayward’, ‘Halla-Gold’, ‘Jecy-Sweet’ and ‘Jecy-Gold’ cultivars, respectively. Two hundred and eighteen isolates were obtained from diseased fruits and identified by mycological and molecular biological methods. Three fungi, Botryspheria dothidea, Diaphorthe actinidiae and Botrytis cinerea, were identified as pathogens of the postharvest fruit rots with detection rates of 95.4%, 4.6% and 2.3%, respectively. 참다래 품종 ‘헤이워드’와 우리나라에서 육성된 품종인 ‘한라골드’, ‘제시골드’, ‘제시스위트’를 동일한 조건에서 재배한 후 후숙 과정에서 발생하는 과실무름병의 발병률을 조사하였다. 발병률은 품종에 따라 큰 차이를 보였다. 품종별 발병률은 ‘헤이워드’ 74.8%, ‘한라골드’ 65.3%, ‘제시스위트’ 57.1%, ‘제시골드’ 16.2% 순으로 조사된 품종 중 ‘제시골드’가 과실무름병에 대한 저항성이 가장 컸다. 병반 부위에서 218개 균주를 분리하여 동정한 결과 과실무름병을 일으키는 병원균은 Botryspheria dothidea, Diaphorthe actinidiae, Botrytis cinerea의 세 종류로 밝혀졌다. 각 병원균의 검출률은 각각 95.4%, 4.6%, 2.3%로 B. dothidea가 과실무름병의 주요 병원균이었다.