Uroskinase 에 의한 항응혈반응에 관한 연구 Ⅰ. Urokinase 의 정제와 효소 특성

박진우,이현재,김수자 ( Jin Woo Park,Hyun Jae Lee,Soo Ja kim ) 생화학분자생물학회 1980 BMB Reports Vol.13 No.3

The activation of plasminogen to an active fibrinolytic enzyme, plasmin, was studied withe urokinase(EC 3. 4. 26. 99). A crude form of urokinase obtained from human urine was purified partially by an ion exchange column of ECTEOLA and further by an affinity column employing arginine or agmatine as a ligand on Sepharose 4B matrix. The specific activity of the final enzyme preparation was about 1, 300 units per ㎎ protein based on the caseinolytic assay measuring the rate of casein hydrolysis by plasmin formed. The mode of the enzyme action seems to be similar to that of trypsin in term of the esterase activity, but the enzyme is found to be very specific toward plasminogen as the substrate. Characteristic properties of the enzyme including molecular weight, pH and temderature optimum, and some kinetic properties were studied and compared with that of the other proteolytic enzyme of trypsin and plasmin. In addition, based on its associative properties, the nature and the physiological role in the fibrinolysis were also discussed.

운모기판을 이용한 다결정 Si 전이막 성장 연구

박진우,엄지혜,안병태,정영권,Park Jin Woo,Eom Ji Hye,Ahn Byung Tae,Jun Young Kwon 한국재료학회 2004 한국재료학회지 Vol.14 No.5

We investigated the growth feasibility of polycrystalline Si film on mica substrate for the transfer of the layer to a plastic substrate. The annealing temperature was limited up to $600^{\circ}C$ because of crack development in the mica substrate. Amorphous Si film was deposited on mica substrate by PECVD and was crystallized by furnace annealing. During the annealing, bubbles were formed at the Si/mica interface. The bubble formation was avoided by the Ar-plasma treatment before amorphous Si deposition. A uniform and clean polycrystalline Si film was obtained by coating $NiCl_2$ on the amorphous Si film and annealing at $500^{\circ}C$ for 10 h. The conventional Si lithography was possible on the mica substrate and the devices fabricated on the substrate could be transferred to a plastic substrate.

탈단백 우골의 골이식 대체재로서의 특성에 대한 평가;세 종류의 골 대체재의 기본 특성에 대한 비교분석

박진우,Park, Jin-Woo 대한치주과학회 2005 Journal of Periodontal & Implant Science Vol.35 No.4

Bovine bone-derived bone substitutes are widely used for treatment of bone defects in dental and orthopedic regenerative surgery. The purpose of this study was to evaluate the basic characteristics of deproteinized bovine bone mineral as a bone graft substitute. Commercially available products from three different bovine bone minerals-Bio-Oss(GeistlichPharma, Switzerland), BBP(Oscotec. Korea), Osteograf/N-300(Dentsply Friadent Ceramed, USA) - were investigated. They were evaluated by scanning electron microscopy(SEM), energy dispersive X-ray spectrometer(EDS), surface area analysis(BET), and Kjeldahl protein analysis. Cell viability on different products was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) assay. The results of this study indicated that each bone substitute displayed distinct surface properties. Furthermore, Kjeldahl protein analysis indicated that residual crude proteins are present in deproteinized bovine bone mineral. BBP showed relatively large amount of residual protein, which indicated that the possibility of disease transmission can not be safely ruled out. Based on the results of this study, it is suggested that active quality management is strongly needed in operations that involve processing bovine bone tissue for medical use.

DADI/MIKE Spectrometry를 이용한 Peptides의 아미노산 배열결정

박진우,Park, Jeen-Woo 생화학분자생물학회 1982 한국생화학회지 Vol.15 No.3

Metastable transition studies로 peptides의 아미노산 배열을 추적하였다. 본 연구에서는 metastable transitions를 확인하기 위해 DADI/MIKE spectrometry를 적용하였다. pentapeptide인 enkephalin 유도체, oligopeptide들의 mixture 및 isomeric peptides mixture에 stepwise DADI/MIKE spectrometry를 적용한 결과 배열결정에 주요한 metastable ionpeaks를 얻을 수 있었다. 이러한 방법으로 얻어진 DADI/MIKE spectra로 용이하게 아미노산 배열을 결정할 수 있었다. Sequence analysis of peptides was investigated by metastable transition studies. In the present work, metastable transitions of peptides were detected by direct analysis of daughter ions (DADI) or mass analyzed ion kinetic energy (MIKE) spectrometry. Stepwise DADI/MIKE spectrometry of enkephalin derivative, oligopeptides mixture, and isomeric peptides mixture were examined. Since prominent metastable ion peaks were observed, it was possible to elucidate peptide sequences from the DADI/MIKE spectra.

국민건강영양조사 표본설계를 위한 추출틀 구축

박진우,변종석,박민규,Park, Jin-Woo,Byun, Jong-Seok,Park, Min-Kyu 한국통계학회 2010 응용통계연구 Vol.23 No.5

제 5기 국민건강영양조사(2010년.2012년)를 위한 표본설계의 여건은 과거에 비해 불리한데, 무엇보다 과거와 같은 추출틀 마련이 여의치 않다는 점이 두드러진다. 제5기 조사를 위한 표본설계 시기가 인구주택총조사 주기와 맞지 않음으로 인해 과거의 총조사 자료를 그대로 추출틀로 사용한다면 어쩔 수 없이 추출틀오차의 문제가 발생하게 될 것이기 때문이다. 이를 극복하기 위한 현실적인 방안을 마련하는 것은 실용적인 면에서 매우 절실한 문제라고 할 수 있다. 본 연구의 목적은 인구주택총조사 자료가 낡아 현재의 모집단 상황을 제대로 대표할 수 없는 상황에서 이를 극복할 수 있는 효과적인 추출틀을 마련하는 데 있다. 본 연구에서는 추출틀의 포함률을 높이기 위한 방안으로 아파트 가구와 일반가구(단독주택, 연립주택, 다세대주택 등)를 분리하여 각각에 대해 서로 다른 자료를 활용하는 방안을 도입한다. 다시 말해 표본설계를 위해 여러 개의 추출틀을 사용하는 방안을 제시한다. One of the problems in designing the fifth Korean National Health and Nutrition Examination Survey(KNHNES) is the lack of an appropriate sampling frame. Due to the significant time difference, we expect eight severe sampling frame errors if we use the sampling frame obtained from the latest population and housing census that was conducted in 2005. Thus, the construction of an appropriate sampling frame for the fifth KNHNES is crucial for a successful survey. We considered the construction of a sampling frame that overcomes the limitations of the 2005 population and housing census based frame. For the construction of eight new sampling frames, we considered the use of multiple sampling frames in which the frame for the apartment households and the frame for the general households are obtained from different sources.

통계기반 정책사례 연구: 주택가격지수 통계의 구축, 개선, 활용을 중심으로

박진우,Park, Jin-Woo 한국통계학회 2009 응용통계연구 Vol.22 No.3

사회가 민주화, 선진화되어감에 따라 합리적인 정책과정의 중요성이 강조되어 이른바, 증거기반 정책(evidence-based policy) 이 정책 분야에서의 중요한 화두로 떠오르게 되었다. 증거기반 정책, 그 중에서도 통계에 기반을 둔 정책에 관한 관심이 고조되고 있기는 하지만 구체적인 정책분야에서 통계가 어떻게 사용되고 있는지에 대한 구체적인 사례들이 소개된 것은 그다지 많지 않다. 본 연구의 목적은, 현재 국민은행이 작성하여 공표하는 주택가격지수가 구축, 발전되어 온 과정을 더듬으면서 구체적으로 통계가 어떻게 주택정책의 기반으로 활용되어 왔는지를 조명하는데 있다. 시기별 주택가격지수의 통계적 특징 및 문제점들을 지적하고, 이러한 문제점들이 개선되는 과정을 살펴본다. 아울러 주택가격지수가 구체적인 부동산 관련 정책과정에서 어떻게 활용 되는지를 소개한다. Democratization and advancement of a society requires the Government's commitment to evidence-based policy. Though statistic is known as one of the best available evidence, there has been only a few case studies to tell real stories about using statistics for policy making. The object of this study is to suggest some real stories about using the Housing Purchase Price Survey for some property policies. By reviewing the origin and development of the survey, we evaluate the design and analysis strategies adopted in the survey. In addition, we describe how the Housing Purchase Price Indices have been used by the Government for some property policies.

깜짝인터뷰-부산 동주대 도서관 김연희 사서

박진우,Park Jin-U 한국도서관협회 2002 圖協會報 Vol.43 No.4

계면 활성제 첨가한 암모니아수의 소수성 실리콘 웨이퍼와의 반응 세정 효과

박진우,박진구,김기섭,송형수,Park, Jin-U,Park, Jin-Gu,Kim, Gi-Seop,Song, Hyeong-Su 한국재료학회 1999 한국재료학회지 Vol.9 No.9

본 연구에서는 NH(sub)4OH에 계면 활성제를 첨가한 세정액의 특성 및 세정 효과를 H(sub)2O(sub)2가 첨가된 NH(sub)4OH 세정액과 비교 연구하였다. NH(sub)4OH에 계면 활성제를 첨가한 경우 용액의 pH 및 산화 환원전위(Eh) 는 NH(sub)4OH의 값과 거의 유사하고 H(sub)2O(sub)2를 첨가한 경우에 pH는 감소하고 Eh는 증가하는 경향을 보였다. 표면장력은 계면 활성제를 첨가한 경우 약 72 dynes/cm에서 약 38dynes/cm로 감소하였으나 H(sub)2O(sub)2를 첨가한 경우에는 NH\ulcornerOH 용액과 비교 거의 변화가 없었다. 실리콘 웨이퍼의 식각 속도를 측정한 결과 H(sub)2O(sub)2나 계면 활성제를 첨가하지 않은 초순수와 1 : 5(NH(sub)4OH : H(sub)2O(sub)2)의 부피비의 NH(sub)4OH용액이 첨가한 용액에 비해 최소 50배 이상의 높은 식각 속도를 보였다. 파티클 제거 실험에서 H(sub)2O(sub)2가 첨가된 NH(sub)4OH 세정액은 실험에 사용한 실리콘 표면위의 직경 0.67$\mu\textrm{m}$의 PSL파티클을 세정액의 온도와 무관하게 잘 제거하였으나 계면 활성제를 첨가한 NH\ulcornerOH경우에 상온에서는 파티클을 제거할 수 없었으나 온도를 5$0^{\circ}C$와 8$0^{\circ}C$로 증가시킨 경우 H(sub)2O(sub)2가 첨가된 NH(sub)4OH와 유사한 세정 효과를 나타내었다. The purpose of this research was to investigate the characteristics and the cleaning efficiency on HN(sub)4OH solutions added with H(sub)2O(sub)2 and surfactants. NH(sub)4OH solutions added with surfactants did not show much changes in pH and redox potential (Eh) as a function of NH(sub)4OH concentration compared with NH(sub)4OH solution. However H(sub)2O(sub)2 added NH\ulcornerOH solutions showed the decrease of pH and the increase of Eh as the concentration of NH(sub)4OH increased. The decrease of surface tension from 72 dynes/cm to 38 dynes/cm was observed in solutions added with surfactant but not in H(sub)2O(sub)2. The etch rates of silicon in NH(sub)4OH solutions(NH(sub)4OH:H(sub)2O= 1 : 5) showed at least 50 times higher than those in H(sub)2O(sub)2 and surfactant added NH(sub)4OH solutions(NH(sub)4OH:H(sub)2O(sub)2= 1 : 1 : 5) solution removed the PSL particles (0.67$\mu\textrm{m}$ in diameter) on Si wafers effectively at all temperatures investigated. NH(sub)4OH solution added with a surfactant could not remove particles at room temperature, however it was possible to remove particles at higher temperatures, 5$0^{\circ}C$ and 8$0^{\circ}C$.

Plasminogen 활성제로서의 Urokinase 의 활성화 부위내 작용기들에 대한 연구

박진우,김수자,이현재 ( Jin Woo Park,Soo Ja Kim,Hyun Jae Lee ) 생화학분자생물학회 1983 BMB Reports Vol.16 No.2

In order to elucidate the mechanism of the urokinase- induced plasminogen activetion, catalytically active functional groups which involved in the active site of urokinase molecule were investigated by a series of kinetic and chemical modification experiments. Inhibition study with several lysine and arginine derivatives indicates there involves hydrophobic interaction between urokinase and plasminogen molecules. 6-Amino hexanoic acid shows an exceptional high degree inhibition. The results from a set of pH-activity profiles and several chamical modification experiments suggest there involve at lease one serine hydroxyl moiety and one histidine imidazole group in the active site of the activator urokinase for the catalytic conversion of plaminogen into plasmin. Based on the results obtained, a minimal three step mechanism of the UK-induced plasminogen activation process was proposed as a working model.

PDGF-BB 적용시간이 decalcified dentin에서의 치은섬유아세포의 증식에 대한 효과

박진우,이재목,서조영,Park, Jin-Woo,Lee, Jae-Mok,Sun, Jo-Young 대한치주과학회 1996 Journal of Periodontal & Implant Science Vol.26 No.4

Platelet-derived growth factor(PDGF) is one of the polypeptide growth fators. PDGF has been reported as a biological mediator which regulates activities of wound healing process including the cell proliferation, migration and metabolism. Recent studies indicated that demineralized root surface as the primary site for growth factor application has advantages over other application method, especially due to binding capacity of growth factor for exposed matrix component of deminera1ized dentin surface. The purpose of this study is to evaluate optimal application time of PDGF-BB on proliferation of human gingival fibroblasts using deminera1ized dentin surface as primary application site. Human gingival fibroblasts and dentin slabs were prepared from the first premolar tooth extracted for the orthodontic treatment, cells were cultured in DMEM/I0% FBS at the $37^{\circ}C$, 5% CO2 incubator. All of the dentin slabs were preconditioned with Tetracycline HCI(100mg/ml) solution and rinsed in PBS. In the cell proliferation experiment, experimental group was immersed in DMEM containing 10% FBS, 50ng/rnl PDGF-BB during different time(30sec, 1, 2, 4, 8 minutes) and dried. Cells at concentration of $1{\times}10^5$cells/ml were seeded in each culture well which contained dentin slabs and incubated for 6 hours. Then, all of the dentin slabs were moved into new 24 well culture dish and incubated for 24, 48, 72 hours. The cell counting was done by hemocytometer with inverted phase contrast microscope after trypsinization. The results were as follows : The application of PDGF-BB for 1, 2 min slightly increased the number of gingival fibroblasts, and the application of PDGF-BB for 4, 8 min prominently increased the number of gingival fibroblasts. The application of PDGF-BB for 4 min showed maximum proliferation rate of gingival fibroblasts at 24, 48, 72 hours, and the application of PDGF-BB for 8 min showed less proliferation rate of gingival fibroblasts compared to the application of PDGF-BB for 4 min at 24, 48, 72 hours. In conclusion, the application of PDGF-BB for 4 min appeared to be optimal to obtain maximum proliferation of gingival fibroblasts using demineralized dentin surface as primary applicaton site of PDGF-BB.