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      • KCI등재

        Expression of Fibroblast Growth Factor Receptors atDifferent Stages of Differentiation inChick Embryo Chondrocytes

        서조영*,박종을*,김우택 대한소아청소년과학회 2004 Clinical and Experimental Pediatrics (CEP) Vol.47 No.4

        Purpose:Proliferative chondrocytes and prehypertrophic chondrocytes secrete significant amounts of type II collagen in an extracellular matrix. In contrast, hypertrophic chondrocytes secrete type X collagen. In addition, fibroblast growth factors (FGFs) and fibroblast growth factor receptors (FGFRs) also appear to play an important role during differentiation. Accordingly, the current study identified and characterized the chondrocytes and FGFR mRNA expressed at different stages of differentiation. Methods:Chondrocytes were isolated from the caudal one-third portion (LS) of the sterna, peripheral regions (USP) and central core regions (USC) of the cephalic portion of the sterna, and the lower portion of the proximal tibial growth plate (Ti). Chondrocytes from the LS, USP, USC, and Ti of 17-day-old chick embryo sterna and tibia were cultured and type II and type X collagen mRNA and FGFR1, FGFR2, and FGFR3 mRNA were isolated and analyzed by Northern blotting. Results:Generally, the cells were larger in size after two days of culture than after seven days of culture and the cells from the USC and Ti were larger and more mature than those from the LS and USP. Type II collagen genes were found to be expressed in all the chondrocyte types, while type X collagen was strongly expressed in the USC and Ti. Therefore, the LS was identified as a resting or proliferative zone, the USP as a postproliferative or prehypertrophic zone, and the USC or Ti as a hypertrophic zone. FGFR1 was expressed only in hypertrophic chondrocytes in proportion to the culture time, FGFR2 was not expressed in any of the chondrocyte types, and FGFR3 was expressed in all the chondrocyte types. Conclusion:As such, it is possible that the different receptors play distinct roles during chondrocyte differentiation 병아리 태아 연골세포의 분화단계에서섬유아세포 성장인자 수용체의 발현경북대학교 치과대학 치주학교실*,대구가톨릭대학교 의과대학 소아과학교실서조영*·박종을*·김우택목 적 : 증식기 및 전비후기 연골세포는 세포외 기질에서 상당히 많은 양의 제 2형 콜라젠을 분비하고 비후기 연골세포는 제 10형 콜라젠을 분비한다. 또한 섬유아세포 성장인자와 섬유아세포 성장인자 수용체도 연골세포 분화에 중요한 역할을 한다. 따라서, 본 연구는 연골세포 분화의 각기 다른 상태에서 연골세포의 특징을 규명하고 섬유아세포 성장인자 수용체의 mRNA의 발현을 알아보고자 하였다. 방 법 : 연골세포를 17일된 태병아리의 흉골 하부 1/3에서, 흉골 상부 말단 부위에서, 흉골 상부 중심부위에서, 그리고 경골 근위부 하방에서 채취하였다. 연골세포를 제 2형 및 제 10형 콜라젠 mRNA와 제 1형, 제 2형, 및 제 3형 섬유아세포 성장인자 수용체의 mRNA의 발현을 관찰하였다.결 과 : 일반적으로 연골세포는 7일간 배양한 것보다 2일간 배양한 것이 크고 흉골 상부 중심부위의 연골세포와 경골 근위부 하방의 연골세포가 흉골 하부 1/3의 연골세포와 흉골 상부 말단 부위의 연골세포 보다 더 크고 성숙되어 있었다. 제 2형 콜라젠 mRNA는 모든 연골세포에서 발현되는 반면에 제 10형 콜라제 mRNA는 흉골 상부 중심부위의 연골세포와 경골 근위부 하방의 연골세포에서 강하게 발현되었다. 따라서 흉골 하부 1/3의 연골세포는 휴지기 및 전증식기 연골세포로, 흉골 상부 말단 부위의 연골세포는 증식기 및 전비후기 연골세포로, 흉골 상부 중심부위의 연골세포와 경골 근위부 하방의 연골세포는 비후기 연골세포로 구분할 수 있다. 제 1형 섬유아세포 성장인자 수용체의 mRNA는 배양 기간에 비례하여 비후기 연골세포에서 발현되었고, 제 2형 섬유아세포 성장인자 수용체의 mRNA는 모든 연골세포에서 발현되지 않았으나, 제 3형 섬유아세포 성장인자 수용체의 mRNA는 모든 연골세포에서 발현되었다.

      • KCI등재
      • KCI등재
      • KCI등재후보

        치주인대세포와 치은섬유아세포의 성상에 관한 비교

        서조영,최제용,유현모,박준봉,조준승 대한구강생물학회 1991 International Journal of Oral Biology Vol.15 No.1

        This study described a characterization of cell types derived from explants of human periodontia. Connective tissue cells for this study were obtained from the healthy gingiva (HGF1, HGF2) and periodontal ligament (PDL1, PDL2, PDL3) of first premolar teeth of individuals undergoing tooth extraction for orthodontic reasons. Cells were studied at passage number 3-7 and were characterized by their cellular DNA content, production of the total protein, collagen, noncollagenous protein and relative collagen synthsis and alkaline phosphatase activity. It was also studied that the effect of parathyroid hormone on the alkaline phosphatase activity, collagen production and production of calcium crystal. The results were as follows: In the comparision of cellular DNA content, the periodontal ligament cells was 2.2-fold higher than the gingival fibroblasts. In the synthetic activity, the protein and collagen production was significantly greater in periodontal ligament cells when compared with that of gingival fibroblasts. In the alkaline phosphatase activity assay, the periodontal ligament cells showed an intense alkaline phosphatase activity both histochemically and biochemically, but gingival fibroblasts or skin fibroblasts did not show. In the study on the effect of the parathyroid hormone on the alkaline phosphatase activity and collagen production, the periodontal ligament cells and gingival fibroblasts failed to show significant reaction. In the Alizarin red S stain for calcium determination, the periodontal ligament cells were positive reaction but gingival fibroblasts were negative reaction. So it was concluded that the periodontal ligament cells exhibit some osteoblast-like characteristics. These findings support the hypothesis that after periodontal therapy, the periodontal ligament cells can form new connective tissue attachment with the newly formed cementum.

      • KCI등재
      • SCIESCOPUSKCI등재

        PDGF와 IGF-I 병용 사용시 치주인대세포의 증식과 세포활성에 미치는 영향에 관한 연구

        서조영,신홍인,경희문,Suh, Jo-Young,Shin, Hong-In,Kyung, Hee-Moon 대한치주과학회 1996 Journal of Periodontal & Implant Science Vol.26 No.2

        Current acceptable methods for promoting periodontal regeneration are based on removal of diseased soft tissue. root treatment, guided tissue regeneration, introduction of new graft materials and biological mediators. Insulin-like growth factor-I(IGF-I) and Platelet-derived growth factor-BB(PDGF-BB), the members of the polypeptuyde growth factor family have been reported as the biological mediators which regulate a variety cellular matrix biologic activities of wound healing process including the cell proliferation, migration and extracellular matrix synthesis.The purposes of this study is to evaluate the combination effects of IGF-I and PDGF-BB on the cellular activity of the periodontal ligament cells to act as a regeneration promoting agent of periodontal tissue. Human periodontal ligament cells were prepared from the first premolar tooth extracted for the orthodontic treatment and were cultured in DMEM containing 10% FBS at the $37^{\circ}C$, 5% CO2 incubator. Author measured the DNA synthetic activity, and total protein, collagen and noncollagenous protein synthetic activities according to the concentration of 10,100ng/ml IGF-I and1,10 ng/ml PDGF-BB in combination. The results were as follows: Significantly increased in the 1 ng/ml PDGF-BB alone compared to the 10 ng/ml PDGF-BB alone(P<0.01) and in the 1 ng/ml PDGF-BB and 10, 100ng/ml IGF-I in combination compared to the 1 ng/ml PDGF-BB alone(P<0.05, P<0.0l). The synthetic activity of the total protein and collagen is significantly increased like to the synthetic activity of the DNA(P<0.05). The synthetic activity of the noncollagenous protein is increased according to the concentration of IGF_I, but not statistically statistically significant(P>0.05). The percent of the collagen is significantly in the 1ng/ml PDGF-BB and 10ng/ml IGF-I in combination compared to the 1ng/ml PDGF-BB alone(P<0.05) and in the 10ng/ml IGF-I in combination compared to the 10ng/ml PDGF-BB alone(P<0.05). The synthetic activity of the DNA is In conclusions, the percent study shows that PDGF-BB and IGF-I in combination have a potentiality to enhance the DNA synthesis and the total protein and collagen synthesis of The periodontal ligament cells, especially it is more significant in the low concentration of PDGF-BB compared to the high one. Thus, the PDGF-BB and IGF-I in combination may have important roles in promotion of periodontal litgment healing, and consequently, may useful for clinical application in periodontal regenerative procedures.

      • 수종의 구강 함수제가 치은 섬유아세포에 미치는 영향

        서조영,박준봉 慶北大學校 齒科大學 1990 慶北齒大論文集 Vol.7 No.1

        세균성 치태의 축적을 경감시키는 목적으로 구강함수제로 사용되는 0.1%와 1% Chlorhexidine, Cetylpyridinium Chloride과 Listerine이 치은 섬유아세포에 미치는 영향을 규명하여 이들 약제들의 생물학적 평가를 도모하고자 시험관적 실험을 실시하였다. 위 약제성분들이 함유된 구강함수제를 구입하여 소독후 100배 희석액을 만들었다. 치은 조직으로부터 분리 배양한 섬유아세포를 60mm 배양접시에 넣어 24시간 전 배양후 상충액을 제거한 다음 준비된 배양액을 재 주입히킨후 24, 48, 72시간동안 37℃ 100 습도 5% Co^2공기 혼합 배양기에서 배양하였다. 배양접시 기저부에 부착된 세포를 trypsin으로 분리하여 그 수를 측정하여 다음과 같은 결과를 얻었다. 시간이 경과함에 따라 세포수의 변화는 Listerine에서는 약간의 감소를 보였으며, 0.1% CHX, CPC, 1% CHX에서는 현저히 감소하였다. 치은 섬유아세포에 대한 독성은 Listerine, 0.1% CHX, CPC, 1% CHX 순으로 강하게 나타났다. 순수 배양액만으로 배양한 경우 24시간까지는 세포가 증식하지 않았으며, 72시간까지는 약2배의 세포 증식을 보였다. The purpose of this study was to evaluate the effect of the four gargling solutions-Listerine, Cetylpyridium chloride(CPC), 0.1% % 1% Chlorhexidine(CHX)-on the human gingival fibroblasts in vitro. The 3×10 exp (5) cells of human gingival fibrobalsts were seeded in each 60mm Petri dish with Dulbecco's Modified Eagle's Medium containing 100u/ml penicillin, 100㎍. ml streptomycin, 1㎍/ml amphotericin-B and 10% fetal bovine serum and incubated for 24 hours. After discard of the supernatant of old medium, those cells cultured with fresh prepared medium at 37℃ 5% Co2/air incubator. The number of cells in the each dish were counted on the hemocytometer after 24, 48, 72 hours incubation. The results were as follows; 1. In the course of time, Listerine was the weak decrease of cell counts and 0.1% CHX, CPC & 1%CHX were the most significant decrease of cell counts in comparision to control cultures. 2. To the cytotoxic effect on the human gingival fibroblast, Listerine, 0.1%CHX, CPC, 1%CHX in order showed more cytotoxic. 3. In the case of culture medium only used, the number of cells were increased two times at 72 hours after seeding. J. Kyungpook Univ. Sch. Dent. Vol. 7, No. 1, 1∼8, 1990.

      • KCI등재후보
      • PDGF와 IGF-I 병용 사용시 치주인대세표의 증식과 세포활성에 미치는 영향에 관한 연구

        서조영,신홍인,경희문 경북대학교 병원 1997 경북대학교병원의학연구소논문집 Vol.1 No.1

        Current acceptable methods for promoting periodontal regeneration are based on removal of diseased soft tissue, root treatment, guided tissue regeneration, introduction of new graft materials and biological mediators. Insulin-like growth factor-I(IGF-I) and Platelet-derived growth factor-BB(PDGF-BB), the members of the polypeptuyde growth factor family have been reported as the biological mediators which regulate a variety cellular matrix biologic activities of wound healing process including the cell proliferation, migration and extracellular matrix synthesis. The purposes of this study is to evaluate the combination effects of IGF-I and PDGF-BB on the cellular activity of the periodontal ligament cells to act as a regeneration promoting agent of periodontal tissue. Human periodontal ligament cells were prepared from the first permolar tooth extracted for the orthodontic treatment and were cultured in DMEM containing 10% FBS at the 37。C, 5% CO2 incubator. Author measured the DNA synthetic activity, and total protein, collagen and noncollagenous protein synthetic activities according to the concentration of 10, 100ng/ml IGF-I and 1, 10 ng/ml PDGF-BB in combination. The results were as follows: significantly increased in the 1 ng/ml PDGF-BB alone compared to the 10 ng/ml PDGF-BB alone(P<0.01)and in the 1 ng/ml PDGF-BB AND 10, 100NG/ML IGF-I in combination compared to the 1ng/ml PDGF-BB alone (P<0.05,P<0.01). The synthetic activity of the noncollagenous protein is increased according to the concentration of IGF_I, but not statistically significant(P>0.05). The percent of collagen is significantly in the 1ng/ml PDGF-BB and 10ng/ml IGF-I in combination compared to the 1ng/ml PDGF-BB alone(P0.05) and in the 10ng/ml IGF-I in combination compared to the 10ng/ml PDGF-BB alone(P<0.05). The synthetic activity of the DNA is in conclusions, the percent study shows that PDGF-BB and IGF-I in combination have a potentiality to enhance the DNA synthesis and the total protein and collagen synthesis of the periodontal ligament cells, especially it is more significant in the low concentration PDGF-BB compared to the high one. Thus, the PDGF-BB and IGF-I in combination may have important roles in promotion of periodontal ligament healing, and consequently, may useful for clinical application in periodontal regenerative procedures.

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