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박수윤,이소영,양정욱,이준설,오성덕,오선우,이시명,임명호,박순기,장재선,조현석,여윤수 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.1
We determined the phytochemical diversity, including carotenoids, flavonoids, anthocyanins, and phenolic acids, in sweet potatoes (Ipomoea batatas L.) with distinctive flesh colors (white, orange, and purple) and identified hydrophilic primary metabolites. Carotenoid content was considerably higher in orange-fleshed sweet potatoes, wherein β-carotene was the most plentiful, and anthocyanins were detected only in purple-fleshed sweet potatoes. The levels of phenolic acids and flavonoids were relatively higher in purple-fleshed sweet potatoes than those in the other two varieties. Forty-one primary and 18 secondary metabolite profiles were subjected to multivariate statistical analyses, which fully distinguished among the varieties and separated orange- and purple-fleshed sweet potatoes from white-fleshed sweet potatoes based on the high levels of sugars, sugar alcohols, and secondary metabolites. This is the first study to determine comprehensive metabolic differences among different color-fleshed sweet potatoes and provides useful information for genetic manipulation of sweet potatoes to influence primary and secondary metabolism.
박수윤,김재광,장재선,이소영,오선우,이시명,양창인,여윤수 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.1
The disease-resistant OsCK1 (Oryza sativa L. cv. Nakdong) was developed in Korea by inserting a choline kinase (CK1) gene into the rice genome. The key nutrients and anti-nutrients of OsCK1 were analyzed and compared with those of its non-transgenic counterpart and four commercial cultivars grown together at two different locations. The levels of proximates, amino acids, fatty acids, minerals, vitamins, trypsin inhibitors, and phytic acid in OsCK1 were comparable to those of its parent rice and the commercial rice varieties. The results of principal component analysis performed using quantification data from 47 nutritional components revealed differences among the rice samples according to their growing locations rather than by their genotypes. These results confirm that the nutritional quality of rice grains was not affected by the insertion of the CK1 gene and suggest that the quality is more affected by environmental factors such as growing conditions than by genetic factors.
박수윤,김재광,김은혜,김승현,프라바카란 마야크리쉬난,정일민 한국작물학회 2018 한국작물학회지 Vol.63 No.4
The levels of 12 isoflavones were measured in soybean (Glycine max (L.) Merrill) sprouts of 68 genetic varieties from three countries (China, Japan, and Korea). The isoflavone profile differences were analyzed using data mining methods. A principal component analysis (PCA) revealed that the CSRV021 variety was separated from the others by the first two principal components. This variety appears to be most suited for functional food production due to its high isoflavone levels. Partial least squares discriminant analysis (PLS-DA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA) showed that there are meaningful isoflavone compositional differences in samples that have different countries of origin. Hierarchical clustering analysis (HCA) of these phytochemicals resulted in clusters derived from closely related biochemical pathways. These results indicate the usefulness of metabolite profiling combined with chemometrics as a tool for assessing the quality of foods and identifying metabolic links in biological systems.