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A Study on the Decondensation and Pronucleus Formation of Sperm Nucleus in the Mouse Oocyte
강희규,김태전,배형준,문희주,이호준,양혜영,김문규 대한의생명과학회 2001 Biomedical Science Letters Vol.7 No.4
To investigate the ability to decondense sperm head penetrated into cytoplasm of the oocytes and the relationship between this ability and the level of glutatione (GSH) in mouse oocyte at various maturing stages. The fertilizability of oocytes at various stages of maturation, the decondensation of sperm nucleus and the formation of male pronucleus, were observed and the levels of GSH were measured in oocyte at same stages. Besides, the relation between fertilizability and level of GSH in oocyte cytoplasm treated with L-buthionine-S, R-sulfoxmine (L-BSO), the inbitor of biosynthesis of GSH, was determined. The decondensation of sperm head was not found in GV stage and L-BSO treated oocytes. In maturing oocytes (GVBD, MI), the decondensation was found, but the formation of male pronucleus was not. The levels of GSH in oocyte cytoplasm were measured; 2.2 pmol per oocyte in the ovulated and the matured in vitro each, 1.0 pmol in GV intact oocyte, 1.3 pmol in GVBD, and 1.5 pmol in MI phase oocyte. In L-BSO treated oocytes the levels of GSH were measured 0.08∼0.09 pmol per oocyte, slightly lower than GV stage oocyte. In conclusion, GSH in oocyte is supposed to be synthesized and storaged in cytoplasm during maturation. The failure of decondensation in the cytoplasm of GV stage and L-BSO treated is suggested that GSH is an essential factor in decondensing the sperm head and that the a certain level of GSH, more than in GV oocyte cytoplasm, is required in de condensation.
조경진,이창규,이승관,정소웅,김태운,문희주,김태전,배형준,최승구,김신무,김종호,진복희 한국보건의료인국가시험원 2004 보건의료교육평가 Vol.1 No.1
Reviewing and comparing the certifying systems for clinical laboratory scientists in some developed countries would be meaningful for us in the renovation of our current systems. Through the homepages of relevant organizations in five countries we got the information on education and certifying systems for clinical laboratory scientists. And we also e-mailed or made phone calls directly to the chairpersons for more additional data. The ladders systems in both educational and certifying were well developed in the United States compared to other countries. In Japan and Korea, it seemed that too many competencies are required for the entry-level medical technologist requiring even competencies in the fields of histo-pathology, cytology, clinical physiology in the current medical technologist' s job descriptions. Now we have to evaluate the applicants' competencies at the entry-level and establish the content outlines as a guideline for the applicants. For more reliable and efficient evaluations we might consider adopting the computerized testing like CBT or CAT.