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넙치에 기생하는 쿠도아 점액포자충(Kudoa septempunctata) 의 활성 확인을 위한 염색법의 비교
도정완(Jeong-Wan DO),문선화(Sun Hwa MOON),김민지(Min Ji KIM),조미영(Mi-Young CHO),정승희(Sung Hee JUNG),이남실(Nam-Sil LEE) 한국수산해양교육학회 2016 水産海洋敎育硏究 Vol.28 No.6
Consumption of Paralichthys olivaceus in raw fish have been reported as the cause of outbreaks of food-born illness, and Kudoa septempunctata in muscle of Paralichtys olivaceus was suggested with the causative agent. For this reason, distinguish of vital and dead spore is important to study survivability of Kudoa septempunctata in human intestinal condition and in vitro inactivation of Kudoa septempunctata. In this reports, we suggest NR & MB (Neutral red and Methylene blue) staining method that is easier and simpler than the previously described HO & PI (Hoechst33342 and Propidium iodide) method according to a experimental condition.
In-situ hybridization 법을 사용한 양식 넙치, Paralichthys olivaceus의 바이러스 감염 질병 특성 고찰
도정완 ( Jeong Wan Do ),이남실 ( Nam Sil Lee ),정승희 ( Sung Hee Jung ),김경길 ( Kyung Kil Kim ),최혜승 ( Hye Sung Choi ),박정우 ( Jeong Woo Park ),김이청 ( Yi Cheong Kim ) 한국어병학회 2013 한국어병학회지 Vol.26 No.3
Polymerase chain reaction (PCR) is the most rapid and widely used method to detect viral pathogens. However, this method does not provide histopathologic nature of the virus. In situ hybridization (ISH) with oligonucleotide probes is attractive because it is a rapid method for detection and identification of viral pathogens at sites of tissue infection. In order to understand the histopathologic characterictics of Red sea bream iridovirus (RSIV), viral-hemorrhagic septicemia (VHS) virus and viral nervous necrosis (VNN) virus to cultured olive flounder, we her applied ISH method to various kinds of olive flounder tissues with PCR-positive for these three viruses. We found that these viruses showed different tissue tropism and were detected from different cell types. Our results suggest that ISH is useful not only in rapid detection of viral pathogens but also in understanding the histopathologic characters of specific viral pathogens.
Aeromonas veronii의 실험적 감염에 의한 양식 메기, Silurus asotus의 병리학적 변화
김진도 ( Jin Do Kim ),도정완 ( Jeong Wan Do ),최혜승 ( Hye Sung Choi ),서정수 ( Jung Soo Seo ),정승희 ( Sung Hee Jung ),조혜인 ( Hyae In Jo ),박명애 ( Myung Ae Park ),이남실 ( Nam Sil Lee ),박성우 ( Sung Woo Park ) 한국환경생물학회 2013 환경생물 : 환경생물학회지 Vol.31 No.4
Recently, a new disease showing symptoms such as epidermal exfoliation and muscular necrosis occurred in cultured Korean catfish. Although the mortality of fishes was low but the economic damages owing to loss of commercial value were severe. The authors isolated the causative agent from diseased fish and observed pathological changes both in naturally and artificially infected fish. The causative bacteria was identified as Aeromonas veronii. Subsequently we observed the daily death and pathological symptoms of artificially infected fish with Aeromonas veronii. Symptoms of artificially infected fish were similar to those of naturally infected fish and all fish died within 7 days after infection. Histopathological changes on the naturally infected fish revealed severe congestion and necrotic degeneration in the liver, spleen and kidney. Some bacterial aggregates with inflammatory degeneration were observed in the heart, and congestion and fibrosis in the lamina propria of digestive tube were predominant. In artificially infected fish, skin erosion and necrotic degeneration of muscle tissue around injected region were particularly manifested. Degeneration of hepatocytes in liver and hyalic degeneration around ellipsoids in spleen were partially observed. However, there were no predominant signs in digestive tube in artificially infected fish.
분자학적, 형태학적 방법을 통한 넙치(Paralichthys olivaceus) 근육에서의 Kudoa septempunctata 감염형태의 변화 관찰
이남실(Nam-Sil LEE),도정완(Jeong-Wan DO),김명석(Myoung Sug KIM),원경미(Kyoungmi WON),조미영(Miyoung CHO),정승희(Sung-Hee JUNG) 한국수산해양교육학회 2019 水産海洋敎育硏究 Vol.31 No.1
This is study about trend of infection form of Kudoa septempunctata in cultured olive flounder (Paralichthys olivaceus). We performed two times of survey (from May 2016 to February 2017 and from May 2017 to September 2017) using PCR method and microscopic method. PCR positive samples had been constantly detected during the survey period, and the prevalence season was from July to October in 2016. However, spore was not detected with microscopic method from May to July, and it had been observed from September 2016 in the 1st survey. These trends are similar in the 2nd survey. In the histopathological examination, partial necrosis of muscle bundle and clump of macrophage around vessel were major findings in muscle tissue that revealed positive result at PCR method only. Moreover, various forms of plasmodial cells were examined from ISH (in-situ hybridization) reaction against muscle tissue that had not K. septempunctata spores.
2010년 한국 연근해 자연산과 양식산 넙치, 참돔, 감성돔, 조피볼락의 병원체 비교
박명애 ( Myoung Ae Park ),도정완 ( Jeung Wan Do ),김명석 ( Myoung Sug Kim ),김석렬 ( Seok Ryel Kim ),권문경 ( Mun Gyeong Kwon ),서정수 ( Jung Soo Seo ),송준영 ( Jun Young Song ),최혜승 ( Hye Sung Choi ) 한국어병학회 2012 한국어병학회지 Vol.25 No.3
This study surveyed for the prevalence of parasites, bacteria and viruses in four fish species, olive flounder (Paralichthys olivaceus), red sea bream (Pagrus major), black sea bream (Acathopagrus schlegeli) and black rockfish (Sebastes schlegeli) in 2010. The survey was aimed to compare the pathogens detected from wild and cultured fish for an epidemiological study. Anisakis sp. was predominantly detected from wild olive flounder and red sea bream (58.6% and 41.7% respectively), but not from the cultured fishes, suggesting anisakid infection is rare in cultured fish. The wild fish get in contact with the anisakids through their prey such as small fishes or crustaceans which carry the anisakids; whereas the cultured fish are fed with formulated feed, free of anisakids. Bacterial detection rates from the wild fishes examined in the study were lower than those of cultured fishes. Vibrio sp. dominated among detected bacterial population in cultured olive flounder (18%). Since vibriosis is known as a secondary infection caused by other stressful factors such as parasitic infections, handling and chemical treatment, it seems that cultured olive flounder are exposed to stressful environment. Viruses diagnosed in the study showed difference in distribution between wild and cultured fishes; hirame rhabdovirus (HRV) (0.1%) and lymphocystis disease virus (LCDV) (3.9%) were detected in the cultured olive flounder, but not in the wild fish, and marine birnavirus (MBV) (1.7%) and red sea bream iridovirus (RSIV) (3.2%) were detected from the wild and cultured red sea bream, respectively. From the survey conducted, it can be concluded that even though some pathogens (Trichodina sp., Microcotyle sp., etc.) are detected from both the wild and cultured fish, pathogens such as Anisakis sp., Vibrio sp. and LCDV showed difference in distribution in the wild and cultured host of same fish species and this can be attributed to their environmental condition and feeding.
2007년∼2011년 하절기에 양식 넙치, 조피볼락, 참돔, 새우의 세균 및 기생충 감염 현황
정승희 ( Sung Hee Jung ),최혜승 ( Hye Sung Choi ),도정완 ( Jeung Wan Do ),김명석 ( Myoung Sug Kim ),권문경 ( Mun Gyeong Kwon ),서정수 ( Jung Soo Seo ),황지연 ( Jee Youn Hwang ),김석렬 ( Seok Ryel Kim ),조영록 ( Yeong Rok Cho ) 한국어병학회 2012 한국어병학회지 Vol.25 No.3
Diagnostic monitoring in fish farms with land-based tanks and netpen cases were conducted in eastern, western, southern and Jeju island of Korea during summer of 2007∼2011. In total, 2413-fish samples of 4 marine fish species were tested for the detection of bacteria and parasite. Fish species tested were olive flounder (Paralichthys olivaceus), black rockfish (Sebastes schlegeli), red sea bream (Pagrus major), pacific white shrimp (Litopenaeus vannamei). During the diagnostic monitoring from 2007 to 2011, the infection rates by single infection of bacterial or parasitic pathogens were relatively higher than the mixed infections. The main bacterial pathogens in olive flounder, black rockfish and pacific white shrimp were Vibrio spp. (V. harveyi, V. ichthyoenteri, Vibrio sp.). The main bacterial pathogens in red sea bream were also Vibrio sp. and Photobacterium damselae subsp. damselae. The main parasitic pathogens were both Miamiensis avidus and Trichodina sp. in olive flounder, Microcotyle sebastes in black rockfish, Microcotyle tai in red sea bream and Zoothamnium sp. in pacific white shrimp.
가물치에 대한 Nocardia seriolae의 인위감염과 조직학적 관찰
이남실(Nam-Sil LEE),한현자(Hyun-Ja HAN),김명석(Myoung-Sug KIM),도정완(Jeong-Wan DO),정승희(Sung-Hee JUNG),조혜인(Hyae-In CHO),김진도(Jin-Do KIM) 한국수산해양교육학회 2016 水産海洋敎育硏究 Vol.28 No.3
Snakehead, Channa argus were intraperitoneally infected with Nocardia seriolae. at the concentrations of 1.5×10<SUP>7</SUP> cfu/㎖ or 1.5×10<SUP>8</SUP> cfu/㎖. The infected fish were kept in aquaria at 25℃ and 30℃ for 3 weeks. Clinical signs and mortality were monitored daily to evaluate the virulence. All artificially infected fish showed the same clinical sign found in naturally infected fish. All the fish infected with 1.5×10<SUP>8</SUP> cfu/㎖ of N. seriolae died within 24days. N. seriolae showed higher virulence to snakehead at the temperature 30℃. Internal lesions such as whitish nodules in the infected internal organs were not correlated with mortality but some degenerative changes were observed in all the infected organs within a week. Whitish nodules in the infected organs which are the typical character in nocardial infection was initially found at two weeks after the artificial infection in snakehead.
양식 넙치 치어에서 분리한 birnavirus 의 특성
손상규(Sang Gyu Sohn),박명애(Myoung Ae Park),도정완(Jeong Wan Do),정초록(Cho Rok Jung),박정우(Jeong Woo Park) 한국어병학회 1995 한국어병학회지 Vol.8 No.2
During 1993 and 1994, some mortalities of flounder(Paralichthy olivaceus) fry were recorded in several fish farms and viruses were isolated from 3 of the farms. Electron microscopic examination revealed that the virus particles were hexagonal and unenveloped with an average diameter of 50 to 55nm. Serological and molecular properties of these isolates were examined. The viral RNA and polypeptides patterns on electrophoresis, as well as neutralization test results, showed that these isolates were birnaviruses and two were closely related to infectious pancreatic necrosis virus(IPNV) serotype AB and one was to IPNV serotype SP. This is the first isolation of birnaviruses from marine fish in Korea