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In-situ hybridization 법을 사용한 양식 넙치, Paralichthys olivaceus의 바이러스 감염 질병 특성 고찰
도정완 ( Jeong Wan Do ),이남실 ( Nam Sil Lee ),정승희 ( Sung Hee Jung ),김경길 ( Kyung Kil Kim ),최혜승 ( Hye Sung Choi ),박정우 ( Jeong Woo Park ),김이청 ( Yi Cheong Kim ) 한국어병학회 2013 한국어병학회지 Vol.26 No.3
Polymerase chain reaction (PCR) is the most rapid and widely used method to detect viral pathogens. However, this method does not provide histopathologic nature of the virus. In situ hybridization (ISH) with oligonucleotide probes is attractive because it is a rapid method for detection and identification of viral pathogens at sites of tissue infection. In order to understand the histopathologic characterictics of Red sea bream iridovirus (RSIV), viral-hemorrhagic septicemia (VHS) virus and viral nervous necrosis (VNN) virus to cultured olive flounder, we her applied ISH method to various kinds of olive flounder tissues with PCR-positive for these three viruses. We found that these viruses showed different tissue tropism and were detected from different cell types. Our results suggest that ISH is useful not only in rapid detection of viral pathogens but also in understanding the histopathologic characters of specific viral pathogens.
넙치에 기생하는 쿠도아 점액포자충(Kudoa septempunctata) 의 활성 확인을 위한 염색법의 비교
도정완(Jeong-Wan DO),문선화(Sun Hwa MOON),김민지(Min Ji KIM),조미영(Mi-Young CHO),정승희(Sung Hee JUNG),이남실(Nam-Sil LEE) 한국수산해양교육학회 2016 水産海洋敎育硏究 Vol.28 No.6
Consumption of Paralichthys olivaceus in raw fish have been reported as the cause of outbreaks of food-born illness, and Kudoa septempunctata in muscle of Paralichtys olivaceus was suggested with the causative agent. For this reason, distinguish of vital and dead spore is important to study survivability of Kudoa septempunctata in human intestinal condition and in vitro inactivation of Kudoa septempunctata. In this reports, we suggest NR & MB (Neutral red and Methylene blue) staining method that is easier and simpler than the previously described HO & PI (Hoechst33342 and Propidium iodide) method according to a experimental condition.
Polymerase Chain Reaction ( PCR ) 을 이용한 Iridovirus 의 검색
도정완(Jeong Wan Do),차승주(Seung Ju Cha),김현주(Hyun Ju Kim),조화자(Wha Ja Cho),문창훈(Chang Hoon Mun),박정민(Jeong Min Park) 한국어병학회 1998 한국어병학회지 Vol.11 No.1
For rapid detection of iridovirus infection, a PCR-based diagnostic method was developed. The genomic DNA from mortality-associated iridovirus was cloned into pUC19 vector. The nucleotide sequences of these clones were compared with sequences of other genes from EMBL/GenBank databank. Based on the nucleotide sequences, PCR primers were prepared and used for PCR. The DNA amplification did not occur from the normal fish cells. In contrast, DNA was amplified from the iridovirus-infected fish cells and purified iridovirus. These results suggest that mortality-associated iridovirus can be detected from virus-infected cells within short time and this PCR-based diagnostic system provides a simple and accurate method for detecting the presence of iridovirus infection.
Aeromonas veronii의 실험적 감염에 의한 양식 메기, Silurus asotus의 병리학적 변화
김진도 ( Jin Do Kim ),도정완 ( Jeong Wan Do ),최혜승 ( Hye Sung Choi ),서정수 ( Jung Soo Seo ),정승희 ( Sung Hee Jung ),조혜인 ( Hyae In Jo ),박명애 ( Myung Ae Park ),이남실 ( Nam Sil Lee ),박성우 ( Sung Woo Park ) 한국환경생물학회 2013 환경생물 : 환경생물학회지 Vol.31 No.4
Recently, a new disease showing symptoms such as epidermal exfoliation and muscular necrosis occurred in cultured Korean catfish. Although the mortality of fishes was low but the economic damages owing to loss of commercial value were severe. The authors isolated the causative agent from diseased fish and observed pathological changes both in naturally and artificially infected fish. The causative bacteria was identified as Aeromonas veronii. Subsequently we observed the daily death and pathological symptoms of artificially infected fish with Aeromonas veronii. Symptoms of artificially infected fish were similar to those of naturally infected fish and all fish died within 7 days after infection. Histopathological changes on the naturally infected fish revealed severe congestion and necrotic degeneration in the liver, spleen and kidney. Some bacterial aggregates with inflammatory degeneration were observed in the heart, and congestion and fibrosis in the lamina propria of digestive tube were predominant. In artificially infected fish, skin erosion and necrotic degeneration of muscle tissue around injected region were particularly manifested. Degeneration of hepatocytes in liver and hyalic degeneration around ellipsoids in spleen were partially observed. However, there were no predominant signs in digestive tube in artificially infected fish.
양식동남아산 뱀장어(Anguilla bicolor pacifica)의 Heterosporis anguillarum 감염
김진도 ( Jin-do Kim ),도정완 ( Jeong-wan Do ),최혜승 ( Hye-sung Choi ),조혜인 ( Hyae-in Jo ),이남실 ( Nam-sil Lee ),김영대 ( Young-dae Kim ) 한국환경생물학회 2014 환경생물 : 환경생물학회지 Vol.32 No.4
Shortfin eel (Anguilla bicolor pacifica) is a species of commercial importance and its production is greatly affected due to the infection by Heterosporis anguillarum. In this study, we evaluated the effect of H. anguillarum infection on the growth of Shortfin eel. A disease that trunk muscle of cultured shortfin eel, Anguilla bicolor pacifica, were irregular and resulted in death, breakout of the commercial eel culture farm. We observed that the trunk muscle of infected eels were irregular and represented white or yellowish externally. Histopathologically, a great numbers of large or small spores and sporophorocysts were also observed in degenerated muscle layer. The cloning of specific gene of H. anguillarum, encoding small subunit ribosomal RNA (SSU-rRNA)was amplified by the polymerase chain reaction(PCR) from the muscle lesion of diseased eel. The size of clone gene is well matched with the size of small subunit ribosomal RNA of H. anguillarum and thus confirming the infection by H. anguillarum.
해상가두리양식장에서 사육한 교잡종 (참돔♀ × 감성돔♂), 긴키돔, 참돔 및 감성돔의 질병 특성
최혜승 ( Hye Sung Choi ),도정완 ( Jeong Wan Do ),박명애 ( Myoung Ae Park ),안철민 ( Chul Min Ahn ) 한국어병학회 2012 한국어병학회지 Vol.25 No.3
Hybrid of red seabream (Pagrus major) and Japanese red seabream (Pagrus major) grew rapidly from to August (WT 19.7℃∼24.0℃) while black seabream (Acanthopagrus major) did from September to October (WT 19.9∼23. 0℃). Survival rate of hybrids (85.5%) was a significantly higher than those of the other fish species (20%). Green liver syndrome were observed in black sea bream and Japanease red seabream but did not in hybrids. Microcotyle tai infected 0∼90% in hybrid, 20∼100% in red seabream, 10% in black sea bream and 20∼100% in Japanese red seabream but no significant difference was found among each fish species.
분자학적, 형태학적 방법을 통한 넙치(Paralichthys olivaceus) 근육에서의 Kudoa septempunctata 감염형태의 변화 관찰
이남실(Nam-Sil LEE),도정완(Jeong-Wan DO),김명석(Myoung Sug KIM),원경미(Kyoungmi WON),조미영(Miyoung CHO),정승희(Sung-Hee JUNG) 한국수산해양교육학회 2019 水産海洋敎育硏究 Vol.31 No.1
This is study about trend of infection form of Kudoa septempunctata in cultured olive flounder (Paralichthys olivaceus). We performed two times of survey (from May 2016 to February 2017 and from May 2017 to September 2017) using PCR method and microscopic method. PCR positive samples had been constantly detected during the survey period, and the prevalence season was from July to October in 2016. However, spore was not detected with microscopic method from May to July, and it had been observed from September 2016 in the 1st survey. These trends are similar in the 2nd survey. In the histopathological examination, partial necrosis of muscle bundle and clump of macrophage around vessel were major findings in muscle tissue that revealed positive result at PCR method only. Moreover, various forms of plasmodial cells were examined from ISH (in-situ hybridization) reaction against muscle tissue that had not K. septempunctata spores.
Amprolium hydrochloride가 넙치 Paralichthys olivaceus의 약물대사 유전자 발현에 미치는 영향
박상협 ( Sang Hyup Park ),김창환 ( Chang Han Kim ),도정완 ( Jeong-wan Do ),최혜승 ( Hye-sung Choi ),김이경 ( Yi Kyung Kim ) 한국어병학회 2023 한국어병학회지 Vol.36 No.2
This study was undertaken to evaluate the effect of amprolium hydrochloride on detoxification process of olive flounder Paralichthys olivaceus. A series of two experiment was performed based on the LD<sub>50</sub> value obtained for amprolium. First, thirty flounder (average weight 230.27 g; average length 27.99 cm) was randomly allocated into five groups. Treatment was carried out using intramuscular injection of amprolium at the dose levels of 4, 8, 16, and 32 mg/kg body weight. At 8, 24 and 48 h post injection, liver and kidney were collected for expression assay of drug metabolizing enzymes and pro-inflammatory cytokine genes. We found that the interleukin-1β (IL-1β) mRNA level were induced at 32 mg/kg and CYP1A genes showed the opposite pattern, while UDP-glucuronosyl-transferase (UGT1A7) and GST were significantly reduced in the liver. Moreover, the suppression of drug metabolizing enzymes and cytokine gene in the kidney was observed after treatment. Another treatment was carried out using intramuscular injection with 4, 8, 16, and 32 mg/kg and 60, 80, 100, 120 mg/kg body weight. At 6 days post injection, liver was collected. The IL-1β expression was markedly induced in the experimental group treated with 4 mg/kg. In addition, glutathione S-transferase (GST) mRNA level was higher in the group with 4 mg/kg. In conclusion, our data suggests that amprolium seem to cause direct or indirect physical, or biological toxicity of flounders, although this drug is considered one of the safest synthetic anticoccidial drugs of the livestock industry.
양식 넙치 치어에서 분리한 birnavirus 의 특성
손상규(Sang Gyu Sohn),박명애(Myoung Ae Park),도정완(Jeong Wan Do),정초록(Cho Rok Jung),박정우(Jeong Woo Park) 한국어병학회 1995 한국어병학회지 Vol.8 No.2
During 1993 and 1994, some mortalities of flounder(Paralichthy olivaceus) fry were recorded in several fish farms and viruses were isolated from 3 of the farms. Electron microscopic examination revealed that the virus particles were hexagonal and unenveloped with an average diameter of 50 to 55nm. Serological and molecular properties of these isolates were examined. The viral RNA and polypeptides patterns on electrophoresis, as well as neutralization test results, showed that these isolates were birnaviruses and two were closely related to infectious pancreatic necrosis virus(IPNV) serotype AB and one was to IPNV serotype SP. This is the first isolation of birnaviruses from marine fish in Korea