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GM 작물의 내건성 검정과 위해성 평가를 위한 가뭄모의시설
남경희 ( Kyong Hee Nam ),고은미 ( Eun Mi Ko ),김도영 ( Do Young Kim ),백인순 ( In Soon Pack ),박정호 ( Jung Ho Park ),김창기 ( Chang Gi Kim ) 한국국제농업개발학회 2014 韓國國際農業開發學會誌 Vol.26 No.3
We designed and constructed a drought-simulation facility for use in producing droughttolerant transgenic crops and assessing their associated risks. This facility consists of soil storage containers and rainout shelters. Each container is equipped with an efficient drainage system so that proper drought conditions can be maintained by controlling water flow to and from the soil. The polyvinyl roof and sidewalls close automatically during rain events to exclude rainfall from plots within the containers but re-open under dry conditions. Water is supplied via sub-surface drip irrigation. The soil water content is monitored at 30-min intervals by sensors installed at depths of 10, 20, 30, and 40 cm. For testing its performance, the field within the facility was divided into two systems, well-watered and water-deficit. Three cultivars of rice were grown. During the experiment, the soil water content was relatively lower in the water-deficit system. Regardless of cultivar, the mean values for plant height; panicle length; grain number, weight, and width; 1000-grain weight; and overall yield were significantly lower in that system when compared with those parameters in the well-watered system.
남경희(Kyong-Hee Nam),김창기(Chang-Gi Kim) 충남대학교 농업과학연구소 2015 농업과학연구 Vol.42 No.1
Drought is one of the major abiotic constraints limiting quantity and quality of rice grain. In order to elucidate the effects of drought on chemical compositions of rice grain, seedlings were cultivated in a rainout shelter controlled with well-watered or water-deficit conditions. After harvesting, the key components including proximates, amino acids, fatty acids, minerals, and vitamins of rice grains were analyzed. Drought stress increased the amounts of methionine, phenylalanine, linoleic acid, linolenic acid, calcium, potassium, vitamin B1, and vitamin E in rice grains, whereas it decreased the contents of lignoceric acid. Particularly, drought stress caused a marked increase in vitamin E content. These results indicate drought significantly influence the chemical compositions of rice grains.
AtCYP78A7 과발현 환경스트레스 내성 형질전환 벼의 단백질 진단 키트 개발
남경희(Kyong-Hee Nam),박정호(Jung-Ho Park),백인순(In-Soon Pack),김호방(Ho Bang Kim),김창기(Chang-Gi Kim) 한국생명과학회 2018 생명과학회지 Vol.28 No.7
본 연구는 시토크롬 P450 단백질을 암호화하는 애기장대 유래의 AtCYP78A7을 과발현하는 형질전환 식물체로 부터 AtCYP78A7 단백질을 특이적으로 인식하는 단일큰론 항체의 제조와 그 항체를 AtCYP78A7 단백질과 접촉시켜 항원-항체 복합체 형성을 검출함으로써 AtCYP78A7 단백질을 효소면역학적(ELISA) 방법으로 검출하는 진단키트를 개발하기 위하여 수행하였다. 재조합한 GST-AtCYP78A7 단백질을 항원으로 사용하여 단일클론 항체를 분비하는 융합세포주를 제조한 후 비오틴화 및 페어링 테스트를 통해 포획항체와 검출항체를 선정하였으며, GST-AtCYP78A7 정제 단백질을 기준으로 일품벼, 화영벼, AtCYP78A7 과발현 벼(10B-5, 18A-4)의 용해물을 검출항원으로 사용하여 product test를 진행하였다. 그 결과 AtCYP78A7 단백질에 특이적으로 결합하는 4개의 단클론 항체(mAb 6A7, mAb 4C2, mAb 11H6, mAb 7E8)를 생산하였고, 포획항체 mAb 4C2와 검출항체 mAb 7E8-biotin의 조합으로 ELISA 키트를 개발하였다. 개발된 ELISA 키트를 이용한 벼 시료의 분석 결과 AtCYP78A7 과발현 벼는 전체 단백질 대비 AtCYP78A7 단백질의 비율이 0.1% 이상인 양성으로, 일품벼와 화영벼는 0.1% 미만인 음성으로 나타나 키트를 이용한 AtCYP78A7 단백질의 검출이 가능하였으며, 따라서 본 키트는 향후 AtCYP78A7를 과발현하는 형질전환 작물을 대상으로 하는 환경 모니터링 또는 인체 위해성 평가에 유용하게 활용될 수 있을 것으로 사료된다. Quantitative determination of the protein expression levels is one of the most important parts in assessment of the safety of foods derived from genetically modified (GM) crops. Overexpression of AtCYP78A7, a gene encoding cytochrome P450 protein, has been reported to improve tolerance to abiotic stress, such as drought and salt stress, in transgenic rice (Oryza sativa L.). In the present study, an enzyme-linked immunosorbent assay (ELISA) kit for diagnosing AtCYP78A7 protein including AtCYP78A7-specific monoclonal antibody was developed. GST-AtCYP78A7 recombinant protein was induced and purified by affinity column. Four monoclonal antibodies (mAb 6A7, mAb 4C2, mAb 11H6, and mAb 7E8) against recombinant protein were also produced and biotinylated with avidin-HRP. After pairing test using GST-AtCYP78A7 protein and lysate of rice samples, mAb 4C2 and mAb 7E8 were selected as a capture antibody and a detecting antibody, respectively, for ELISA kit. Product test using rice samples indicated that percentages of detected protein in total protein were greater than 0.1% in AtCYP78A7-overexpressing transgenic rice (Line 10B-5 and 18A-4), whereas those in negative control non-transgenic rice (Ilpum and Hwayoung) were less than 0.1%. The ELISA kit developed in this study can be useful for the rapid detection and safety assessment of transgenic rice overexpressing AtCYP78A7.
3차원 구조광 스캐너를 이용한 식물의 잎 면적 측정 방법
남경희 ( Kyong Hee Nam ),고은미 ( Eun Mi Ko ),문새로미 ( Saeromi Mun ),김창기 ( Chang Gi Kim ) 한국하천호수학회 2014 생태와 환경 Vol.47 No.3
We have developed a non-destructive, touch-free method for estimating leaf areas with a structuredlight three-dimensional (3D) scanner. When the surfaces of soybean leaves were analyzed with both the 3D scanner and a leaf area meter, the results were linearly related (R2=0.90). The strong correlation (R2=0.98) was calculated between shoot fresh weights and leaf areas when the scanner was employed during growth stages V1 to V4. We also found that leaf areas measured by the scanner could be used to detect changes in growth responses to abiotic stress. Whereas under control conditions the areas increased over time, salt and drought treatments were associated with reductions in those values after 14 d and 12 d, respectively. Based on our findings, we propose that a structured-light 3D scanner can be used to obtain reliable estimates of leaf area and plant biomass.
유전자변형 양배추로부터 비유전자변형 모본으로의 유전자 이동성
김영중(Young-Joong Kim),남경희(Kyong Hee Nam),백인순(In Soon Pack),박정호(Jung-Ho Park),정순천(Soon-Chun Jeong),한지학(Chee Hark Harn),김창기(Chang-Gi Kim) 충남대학교 농업과학연구소 2014 농업과학연구 Vol.41 No.3
Understanding the gene flow from genetically modified (GM) crops to conventional crops is important to prevent and mitigate seed contamination caused by pollen-mediated gene flow. We conducted a field test to investigate the gene flow from diamondback moth resistant GM cabbage (Brassica oleracea var. capitata) containing cry1Ac1 gene, to a non-GM control line AD126. GM and non-GM cabbage plants were cultivated in the field and pollinated using Bombus terrestris under the nets during the flowering periods. After seeds were collected from non-GM plants, hybrids between them and the GM cabbages were screened by multiplex PCR targeting cry1Ac1 gene. Out of 878 germinated seedlings, 168 hybrids were found and the average gene flow frequency was 19.7%. Because cabbage is mainly pollinated by insect pollinators, large-scale field tests are needed to study gene flow of GM cabbage.
Bt 양배추가 복숭아혹진딧물의 발육과 기주선택에 미치는 영향
남기정,김영중,문두범,남경희,백인순,박정호,정순천,한지학,김창기,Nam, Ki Jung,Kim, Young-Joong,Moon, Doo-Bum,Nam, Kyong-Hee,Pack, In Soon,Park, Jung-Ho,Jeong, Soon-Chun,Harn, Chee Hark,Kim, Chang-Gi 한국응용곤충학회 2014 한국응용곤충학회지 Vol.53 No.2
해충저항성 유전자변형 작물은 대상 해충에 대한 방제 효과를 증진시킬 수 있는 효과적인 수단이 될 수 있지만, 생태계 내의 다른 비표적 생물에 부정적인 영향을 줄 가능성 또한 있기 때문에 상업적인 재배에 앞서 이에 대한 충분한 연구가 이루어져야 한다. 본 연구에서는 배추좀나방내성 유전자변형 Bt (Cry1Ac1)양배추가 비표적 곤충인 복숭아혹진딧물에 미치는 영향을 알아보기 위한 기초적인 실험으로서 기주선택실험 및 성장실험을 실시하였다. 기주선택실험에서, 두 개체씩의 Bt 양배추와 모본 양배추에 각각 10마리의 유시 성충(alate virginoparae)을 접종하고, 사흘간 생식(reproduction) 기회를 제공한 후 생산한 약충의 마리수를 측정한 결과, Bt 양배추와 모본 양배추에 낳은 약충의 수가 각각 $21.9{\pm}1.8$와 $22.5{\pm}2.2$로 비슷하였다. 성장실험에서, 갓 태어난 무시 약충(apterous virginoparae)을 Bt 양배추 및 모본 양배추를 기주로 하여 키웠을 때, 성충이 될 때까지 걸린 시간은 Bt 양배추와 모본 양배추에서 각각 $5.8{\pm}0.2$, $5.9{\pm}0.1$ 일(day)로 통계적으로 유의한 차이가 없었고, 개체군 생장률(rm) 또한 $0.7{\pm}0.1$, $0.8{\pm}0.1$로 유사하였다. 이상의 결과들로 볼때 Bt 양배추가 복숭아혹진딧물에 미치는 영향이 없거나 미미함을 알 수 있으나, 다양한 관점에서의 보완 연구가 더 필요하다. Transgenic crops that produce insecticidal toxins have a great potential for controlling target pest insects, but there is a growing concern about unintended influences on non-target species. In the present study, the preferences and performance of non-target green peach aphid, Myzus persicae (Sulzer), on transgenic cabbages (Brassica oleracea) that produce Bt toxin (Cry1Ac1) and untransformed control plants were investigated as a part of risk assessment. In a free-choice situation, the number of nymphs larviposited by 10 winged adults over 3 days was $21.9{\pm}1.8$ and $22.5{\pm}2.2$ on transgenic and the control plants, respectively, indicating that the aphids did not discriminate between the two types of plants. In a performance assay, the development time (D) and intrinsic rate of increase (rm) of wingless aphids reared on transgenic and control plants were also similar (D, $5.8{\pm}0.2$ and $5.9{\pm}0.1$ (days) and rm, $0.7{\pm}0.1$ and $0.8{\pm}0.1$, for transgenic and control plants, respectively). These results suggest that M. persicae is not significantly affected by transgenic Bt cabbage.
연구논문 : 자연과학 ; 유전자변형 유채의 환경방출실험에서 격리포장 외부로의 도입유전자 이동가능성 모니터링
김도영 ( Do Young Kim ),남기정 ( Ki Jung Nam ),문예슬 ( Ye Seul Moon ),김대인 ( Dae In Kim ),김영중 ( Young Joong Kim ),문두범 ( Doo Bum Moon ),천영진 ( Young Jin Chun ),남경희 ( Kyong Hee Nam ),안주희 ( Joo Hee An ),이범규 ( Bum 한국국제농업개발학회 2012 韓國國際農業開發學會誌 Vol.24 No.2
1. 본 연구에서는 GM 유채(Brassica napus)의 환경방출실험시의 안전관리를 위한 모니터링을 수행하였다. 2. 문헌조사를 통해 유채의 품종 순도 유지를 위한 격리거리와 교잡가능한 종을 확인함으로써 모니터링의 범위와 모니터링 대상 식물종을 결정하였다. 3. GM 유채의 격리포장으로부터 1.3 km 거리 범위 내에서 발견된 B. napus, B. juncea 및 B. oleracea를 대상으로 모니터링을 실시한 결과, 교잡을 통한 유전자의 유출은 발견되지 않았다. One of the main concerns in field trials of GM crops is transgene escape from GM crops to conventional crops or weedy (wild) relatives via pollen dispersal. The management of field trials is therefore aimed at the detection and prevention of hybridization between GM crops and their closely related species around the field. This study monitored the escape of introduced genes of GM oilseed rapes (Brassica napus) outside the trial site during a small-scale field trial. First, a monitoring area (up to 1.3 km from the isolated field site) was determined according to recommended isolation distances for maintaining varietal purities of oilseed rapes. Then, target species (B. napus, B. juncea and B. oleracea) for monitoring were chosen using a literature search of plant species reproductively compatible with oilseed rapes. Hybrids between GM oilseed rapes and sexually compatible species were not detected and this result indicated that transgene escape outside the trial site did not occur during a field trial.
연구논문 : 한국의 외래생물 관리 현황 및 개선방향: 일본 외래종 법제와의 비교 검토를 중심으로
문새로미 ( Sae Ro Mi Mun ),남경희 ( Kyong Hee Nam ),김창기 ( Chang Gi Kim ),천영진 ( Young Jin Chun ),이현우 ( Hyun Woo Lee ),길지현 ( Ji Hyun Kil ),이종천 ( Jong Chun Lee ) 한국환경정책학회 2013 環境政策 Vol.21 No.2
Introduction, establishment and invasion of alien species have caused worldwide environmental losses. To prevent the adverse effects of invasive alien species on ecosystems, human safety and agriculture, forestry and fisheries, Japan has enacted the Invasive Alien Species Act since 2005. Invasive Alien Species (IAS) and Uncategorized Alien Species (UAS) have been designated based on the potential adverse risks of alien species on ecosystems in Japan. The regulation on IAS and UAS have greatly reduced the importation of live wildlife in Japan, and these regulation system have been considered effective for reducing the introduction of alien species into Japan. In Korea, the harmful non-indigenous species has been regulated by the Natural Environment Conservation Act since 1998. According to the Act on Preservation and Use of Biodiversity Act (Biodiversity Act) that has been entered into force in February 2013, ecological risk assessment should be conducted for the import of potentially harmful alien species. This Act also covers the regulation of the alien species that seriously damage ecosystems (ecosystem-disturbing species), previously designated as harmful non-indigenous species. The procedures for the designation, import approval of the potentially harmful alien species and communication with stakeholder need to be improved for the efficient enforcement of the newBiodiversity Act in Korea.